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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
MAST2 KIAA0807, MAST205
Sequence Length (AA) Molecular Weight (Da)
1798 196436
Protein Name
Microtubule-associated serine/threonine-protein kinase 2
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MKRSRCRDRP QPPPPDRRED GVQRAAELSQ SLPPRRRAPP GRQRLEERTG
60 70 80 90 100
PAGPEGKEQD VVTGVSPLLF RKLSNPDIFS STGKVKLQRQ LSQDDCKLWR
110 120 130 140 150
GNLASSLSGK QLLPLSSSVH SSVGQVTWQS SGEASNLVRM RNQSLGQSAP
160 170 180 190 200
SLTAGLKELS LPRRGSFCRT SNRKSLIVTS STSPTLPRPH SPLHGHTGNS
210 220 230 240 250
PLDSPRNFSP NAPAHFSFVP ARRTDGRRWS LASLPSSGYG TNTPSSTVSS
260 270 280 290 300
SCSSQEKLHQ LPFQPTADEL HFLTKHFSTE SVPDEEGRQS PAMRPRSRSL
310 320 330 340 350
SPGRSPVSFD SEIIMMNHVY KERFPKATAQ MEERLAEFIS SNTPDSVLPL
360 370 380 390 400
ADGALSFIHH QVIEMARDCL DKSRSGLITS QYFYELQDNL EKLLQDAHER
410 420 430 440 450
SESSEVAFVM QLVKKLMIII ARPARLLECL EFDPEEFYHL LEAAEGHAKE
460 470 480 490 500
GQGIKCDIPR YIVSQLGLTR DPLEEMAQLS SCDSPDTPET DDSIEGHGAS
510 520 530 540 550
LPSKKTPSEE DFETIKLISN GAYGAVFLVR HKSTRQRFAM KKINKQNLIL
560 570 580 590 600
RNQIQQAFVE RDILTFAENP FVVSMFCSFD TKRHLCMVME YVEGGDCATL
610 620 630 640 650
LKNIGALPVD MVRLYFAETV LALEYLHNYG IVHRDLKPDN LLITSMGHIK
660 670 680 690 700
LTDFGLSKIG LMSLTTNLYE GHIEKDAREF LDKQVCGTPE YIAPEVILRQ
710 720 730 740 750
GYGKPVDWWA MGIILYEFLV GCVPFFGDTP EELFGQVISD EIVWPEGDEA
760 770 780 790 800
LPPDAQDLTS KLLHQNPLER LGTGSAYEVK QHPFFTGLDW TGLLRQKAEF
810 820 830 840 850
IPQLESEDDT SYFDTRSERY HHMDSEDEEE VSEDGCLEIR QFSSCSPRFN
860 870 880 890 900
KVYSSMERLS LLEERRTPPP TKRSLSEEKE DHSDGLAGLK GRDRSWVIGS
910 920 930 940 950
PEILRKRLSV SESSHTESDS SPPMTVRRRC SGLLDAPRFP EGPEEASSTL
960 970 980 990 1000
RRQPQEGIWV LTPPSGEGVS GPVTEHSGEQ RPKLDEEAVG RSSGSSPAME
1010 1020 1030 1040 1050
TRGRGTSQLA EGATAKAISD LAVRRARHRL LSGDSTEKRT ARPVNKVIKS
1060 1070 1080 1090 1100
ASATALSLLI PSEHHTCSPL ASPMSPHSQS SNPSSRDSSP SRDFLPALGS
1110 1120 1130 1140 1150
MRPPIIIHRA GKKYGFTLRA IRVYMGDSDV YTVHHMVWHV EDGGPASEAG
1160 1170 1180 1190 1200
LRQGDLITHV NGEPVHGLVH TEVVELILKS GNKVAISTTP LENTSIKVGP
1210 1220 1230 1240 1250
ARKGSYKAKM ARRSKRSRGK DGQESRKRSS LFRKITKQAS LLHTSRSLSS
1260 1270 1280 1290 1300
LNRSLSSGES GPGSPTHSHS LSPRSPTQGY RVTPDAVHSV GGNSSQSSSP
1310 1320 1330 1340 1350
SSSVPSSPAG SGHTRPSSLH GLAPKLQRQY RSPRRKSAGS IPLSPLAHTP
1360 1370 1380 1390 1400
SPPPPTASPQ RSPSPLSGHV AQAFPTKLHL SPPLGRQLSR PKSAEPPRSP
1410 1420 1430 1440 1450
LLKRVQSAEK LAAALAASEK KLATSRKHSL DLPHSELKKE LPPREVSPLE
1460 1470 1480 1490 1500
VVGARSVLSG KGALPGKGVL QPAPSRALGT LRQDRAERRE SLQKQEAIRE
1510 1520 1530 1540 1550
VDSSEDDTEE GPENSQGAQE LSLAPHPEVS QSVAPKGAGE SGEEDPFPSR
1560 1570 1580 1590 1600
DPRSLGPMVP SLLTGITLGP PRMESPSGPH RRLGSPQAIE EAASSSSAGP
1610 1620 1630 1640 1650
NLGQSGATDP IPPEGCWKAQ HLHTQALTAL SPSTSGLTPT SSCSPPSSTS
1660 1670 1680 1690 1700
GKLSMWSWKS LIEGPDRASP SRKATMAGGL ANLQDLENTT PAQPKNLSPR
1710 1720 1730 1740 1750
EQGKTQPPSA PRLAHPSYED PSQGWLWESE CAQAVKEDPA LSITQVPDAS
1760 1770 1780 1790 1798
GDRRQDVPCR GCPLTQKSEP SLRRGQEPGG HQKHRDLALV PDELLKQT

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node
to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-5658 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAST2
Peptide Sequence
EDPALSITQVPDASGDR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
3
Peptide Start
1737
Peptide End
1753
CPTAC ID
non-CPTAC-5658
Peptide Molecular Mass
1,769.8432
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
SUM159 tryptic digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Q-Exactive
Internal Standard
25fmol
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
EASY-nLC 1000
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 0.9 2.1 5.2 2.2 2.1 5.4 2.4 3 7.5 15 15 15
y10 (1+) 1.5 1.4 4.1 2.4 2 6.6 2.8 2.4 7.8 15 15 15
y12 (1+) 1.8 1.4 3.9 3 1.7 5.1 3.5 2.2 6.4 15 15 15
sum 0.9 1 3.3 2.3 1.5 3.8 2.5 1.8 5 15 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-5659 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAST2
Peptide Sequence
VAISTTPLENTSIK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
3
Peptide Start
1184
Peptide End
1197
CPTAC ID
non-CPTAC-5659
Peptide Molecular Mass
1,472.8086
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
SUM159 tryptic digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Q-Exactive
Internal Standard
25fmol
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
EASY-nLC 1000
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (1+) 5 2.5 4 6 2.5 4 7.8 3.5 5.7 15 15 15
y10 (1+) 4.9 5.1 9.2 6.2 5.3 9.8 7.9 7.4 13.4 15 15 15
y11 (1+) 5.6 2.4 6.6 7 2.9 8.2 9 3.8 10.5 15 15 15
sum 5 2.3 3.3 6.1 2.6 3.5 7.9 3.5 4.8 15 15 15


Additional Resources and Comments