ADCK5, CPTAC-5660 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
ADCK5	N/A

Sequence Length (AA)	Molecular Weight (Da)
580	65828

Protein Name
Uncharacterized aarF domain-containing protein kinase 5

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MWRPVQLCHF	HSALLHSRQK	PWPSPAVFFR	RNVRGLPPRF	SSPTPLWRKV
60	70	80	90	100
LSTAVVGAPL	LLGARYVMAE	AREKRRMRLV	VDGMGRFGRS	LKVGLQISLD
110	120	130	140	150
YWWCTNVVLR	GVEENSPGYL	EVMSACHQRA	ADALVAGAIS	NGGLYVKLGQ
160	170	180	190	200
GLCSFNHLLP	PEYTRTLRVL	EDRALKRGFQ	EVDELFLEDF	QALPHELFQE
210	220	230	240	250
FDYQPIAAAS	LAQVHRAKLH	DGTSVAVKVQ	YIDLRDRFDG	DIHTLELLLR
260	270	280	290	300
LVEVMHPSFG	FSWVLQDLKG	TLAQELDFEN	EGRNAERCAR	ELAHFPYVVV
310	320	330	340	350
PRVHWDKSSK	RVLTADFCAG	CKVNDVEAIR	SQGLAVHDIA	EKLIKAFAEQ
360	370	380	390	400
IFYTGFIHSD	PHPGNVLVRK	GPDGKAELVL	LDHGLYQFLE	EKDRAALCQL
410	420	430	440	450
WRAIILRDDA	AMRAHAAALG	VQDYLLFAEM	LMQRPVRLGQ	LWGSHLLSRE
460	470	480	490	500
EAAYMVDMAR	ERFEAVMAVL	RELPRPMLLV	LRNINTVRAI	NVALGAPVDR
510	520	530	540	550
YFLMAKRAVR	GWSRLAGATY	RGVYGTSLLR	HAKVVWEMLK	FEVALRLETL
560	570	580
AMRLTALLAR	ALVHLSLVPP	AEELYQYLET

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for non-CPTAC-5660 Collapse assay details

Data source: Panorama

Official Gene Symbol: ADCK5
Peptide Sequence: AINVALGAPVDR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: 3
Peptide Start: 489
Peptide End: 500

CPTAC ID: non-CPTAC-5660
Peptide Molecular Mass: 1,194.6721
Species: Homo sapiens (Human)
Assay Type: Direct PRM
Matrix: SUM159 tryptic digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Q-Exactive
Internal Standard: 25fmol
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: EASY-nLC 1000
Column Packing: C18
Column Dimensions: 75 µm x 50 cm
Flow Rate: 300nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y8 (1+)	2.1	4.4	3.9	3.3	5.4	4.7	3.9	7	6.1	15	15	15
y7 (1+)	1.6	3.7	5.5	4.5	5.8	5.3	4.8	6.9	7.6	15	15	15
y6 (1+)	2.3	5.3	4.8	4.1	5.1	5.2	4.7	7.4	7.1	15	15	15
sum	1.4	4.4	3.7	3.4	5	3.7	3.7	6.7	5.2	15	15	15

ADCK5

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for non-CPTAC-5660 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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ADCK5

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for non-CPTAC-5660 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments