LRRK1, CPTAC-5661 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
LRRK1	KIAA1790

Sequence Length (AA)	Molecular Weight (Da)
2015	225393

Protein Name
Leucine-rich repeat serine/threonine-protein kinase 1

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MAGMSQRPPS	MYWCVGPEES	AVCPERAMET	LNGAGDTGGK	PSTRGGDPAA
60	70	80	90	100
RSRRTEGIRA	AYRRGDRGGA	RDLLEEACDQ	CASQLEKGQL	LSIPAAYGDL
110	120	130	140	150
EMVRYLLSKR	LVELPTEPTD	DNPAVVAAYF	GHTAVVQELL	ESLPGPCSPQ
160	170	180	190	200
RLLNWMLALA	CQRGHLGVVK	LLVLTHGADP	ESYAVRKNEF	PVIVRLPLYA
210	220	230	240	250
AIKSGNEDIA	IFLLRHGAYF	CSYILLDSPD	PSKHLLRKYF	IEASPLPSSY
260	270	280	290	300
PGKTALRVKW	SHLRLPWVDL	DWLIDISCQI	TELDLSANCL	ATLPSVIPWG
310	320	330	340	350
LINLRKLNLS	DNHLGELPGV	QSSDEIICSR	LLEIDISSNK	LSHLPPGFLH
360	370	380	390	400
LSKLQKLTAS	KNCLEKLFEE	ENATNWIGLR	KLQELDISDN	KLTELPALFL
410	420	430	440	450
HSFKSLNSLN	VSRNNLKVFP	DPWACPLKCC	KASRNALECL	PDKMAVFWKN
460	470	480	490	500
HLKDVDFSEN	ALKEVPLGLF	QLDALMFLRL	QGNQLAALPP	QEKWTCRQLK
510	520	530	540	550
TLDLSRNQLG	KNEDGLKTKR	IAFFTTRGRQ	RSGTEAASVL	EFPAFLSESL
560	570	580	590	600
EVLCLNDNHL	DTVPPSVCLL	KSLSELYLGN	NPGLRELPPE	LGQLGNLWQL
610	620	630	640	650
DTEDLTISNV	PAEIQKEGPK	AMLSYLRAQL	RKAEKCKLMK	MIIVGPPRQG
660	670	680	690	700
KSTLLEILQT	GRAPQVVHGE	ATIRTTKWEL	QRPAGSRAKV	ESVEFNVWDI
710	720	730	740	750
GGPASMATVN	QCFFTDKALY	VVVWNLALGE	EAVANLQFWL	LNIEAKAPNA
760	770	780	790	800
VVLVVGTHLD	LIEAKFRVER	IATLRAYVLA	LCRSPSGSRA	TGFPDITFKH
810	820	830	840	850
LHEISCKSLE	GQEGLRQLIF	HVTCSMKDVG	STIGCQRLAG	RLIPRSYLSL
860	870	880	890	900
QEAVLAEQQR	RSRDDDVQYL	TDRQLEQLVE	QTPDNDIKDY	EDLQSAISFL
910	920	930	940	950
IETGTLLHFP	DTSHGLRNLY	FLDPIWLSEC	LQRIFNIKGS	RSVAKNGVIR
960	970	980	990	1000
AEDLRMLLVG	TGFTQQTEEQ	YFQFLAKFEI	ALPVANDSYL	LPHLLPSKPG
1010	1020	1030	1040	1050
LDTHGMRHPT	ANTIQRVFKM	SFVPVGFWQR	FIARMLISLA	EMDLQLFENK
1060	1070	1080	1090	1100
KNTKSRNRKV	TIYSFTGNQR	NRCSTFRVKR	NQTIYWQEGL	LVTFDGGYLS
1110	1120	1130	1140	1150
VESSDVNWKK	KKSGGMKIVC	QSEVRDFSAM	AFITDHVNSL	IDQWFPALTA
1160	1170	1180	1190	1200
TESDGTPLME	QYVPCPVCET	AWAQHTDPSE	KSEDVQYFDM	EDCVLTAIER
1210	1220	1230	1240	1250
DFISCPRHPD	LPVPLQELVP	ELFMTDFPAR	LFLENSKLEH	SEDEGSVLGQ
1260	1270	1280	1290	1300
GGSGTVIYRA	RYQGQPVAVK	RFHIKKFKNF	ANVPADTMLR	HLRATDAMKN
1310	1320	1330	1340	1350
FSEFRQEASM	LHALQHPCIV	ALIGISIHPL	CFALELAPLS	SLNTVLSENA
1360	1370	1380	1390	1400
RDSSFIPLGH	MLTQKIAYQI	ASGLAYLHKK	NIIFCDLKSD	NILVWSLDVK
1410	1420	1430	1440	1450
EHINIKLSDY	GISRQSFHEG	ALGVEGTPGY	QAPEIRPRIV	YDEKVDMFSY
1460	1470	1480	1490	1500
GMVLYELLSG	QRPALGHHQL	QIAKKLSKGI	RPVLGQPEEV	QFRRLQALMM
1510	1520	1530	1540	1550
ECWDTKPEKR	PLALSVVSQM	KDPTFATFMY	ELCCGKQTAF	FSSQGQEYTV
1560	1570	1580	1590	1600
VFWDGKEESR	NYTVVNTEKG	LMEVQRMCCP	GMKVSCQLQV	QRSLWTATED
1610	1620	1630	1640	1650
QKIYIYTLKG	MCPLNTPQQA	LDTPAVVTCF	LAVPVIKKNS	YLVLAGLADG
1660	1670	1680	1690	1700
LVAVFPVVRG	TPKDSCSYLC	SHTANRSKFS	IADEDARQNP	YPVKAMEVVN
1710	1720	1730	1740	1750
SGSEVWYSNG	PGLLVIDCAS	LEICRRLEPY	MAPSMVTSVV	CSSEGRGEEV
1760	1770	1780	1790	1800
VWCLDDKANS	LVMYHSTTYQ	LCARYFCGVP	SPLRDMFPVR	PLDTEPPAAS
1810	1820	1830	1840	1850
HTANPKVPEG	DSIADVSIMY	SEELGTQILI	HQESLTDYCS	MSSYSSSPPR
1860	1870	1880	1890	1900
QAARSPSSLP	SSPASSSSVP	FSTDCEDSDM	LHTPGAASDR	SEHDLTPMDG
1910	1920	1930	1940	1950
ETFSQHLQAV	KILAVRDLIW	VPRRGGDVIV	IGLEKDSGAQ	RGRVIAVLKA
1960	1970	1980	1990	2000
RELTPHGVLV	DAAVVAKDTV	VCTFENENTE	WCLAVWRGWG	AREFDIFYQS
2010	2015
YEELGRLEAC	TRKRR

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below

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Assay Details for non-CPTAC-5626 Collapse assay details

Data source: Panorama

Official Gene Symbol: LRRK1
Peptide Sequence: VTIYSFTGNQR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: 3
Peptide Start: 1060
Peptide End: 1070

CPTAC ID: non-CPTAC-5626
Peptide Molecular Mass: 1,284.6463
Species: Homo sapiens (Human)
Assay Type: Direct PRM
Matrix: SUM159 tryptic digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Q-Exactive
Internal Standard: 25fmol
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: EASY-nLC 1000
Column Packing: C18
Column Dimensions: 75 µm x 50 cm
Flow Rate: 300nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y6 (1+)	12.2	4.1	4.4	12.7	3.8	4.4	17.6	5.6	6.2	15	15	15
y7 (1+)	5.2	1.9	3.5	5.1	3.8	3.6	7.3	4.2	5	15	15	15
y8 (1+)	5.1	3.8	2.9	6.8	4.5	3.4	8.5	5.9	4.5	15	15	15
sum	5.3	2.9	3.3	6	3.7	3.4	8	4.7	4.7	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

Comments

Assay Details for non-CPTAC-5661 Collapse assay details

Data source: Panorama

Official Gene Symbol: LRRK1
Peptide Sequence: YFIEASPLPSSYPGK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: 3
Peptide Start: 239
Peptide End: 253

CPTAC ID: non-CPTAC-5661
Peptide Molecular Mass: 1,654.8243
Species: Homo sapiens (Human)
Assay Type: Direct PRM
Matrix: SUM159 tryptic digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend