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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

PIP5K1A

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
PIP5K1A N/A
Sequence Length (AA) Molecular Weight (Da)
562 62633
Protein Name
Phosphatidylinositol 4-phosphate 5-kinase type-1 alpha
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MASASSGPSS SVGFSSFDPA VPSCTLSSAA SGIKRPMASE VLEARQDSYI
60 70 80 90 100
SLVPYASGMP IKKIGHRSVD SSGETTYKKT TSSALKGAIQ LGITHTVGSL
110 120 130 140 150
STKPERDVLM QDFYVVESIF FPSEGSNLTP AHHYNDFRFK TYAPVAFRYF
160 170 180 190 200
RELFGIRPDD YLYSLCSEPL IELCSSGASG SLFYVSSDDE FIIKTVQHKE
210 220 230 240 250
AEFLQKLLPG YYMNLNQNPR TLLPKFYGLY CVQAGGKNIR IVVMNNLLPR
260 270 280 290 300
SVKMHIKYDL KGSTYKRRAS QKEREKPLPT FKDLDFLQDI PDGLFLDADM
310 320 330 340 350
YNALCKTLQR DCLVLQSFKI MDYSLLMSIH NIDHAQREPL SSETQYSVDT
360 370 380 390 400
RRPAPQKALY STAMESIQGE ARRGGTMETD DHMGGIPARN SKGERLLLYI
410 420 430 440 450
GIIDILQSYR FVKKLEHSWK ALVHDGDTVS VHRPGFYAER FQRFMCNTVF
460 470 480 490 500
KKIPLKPSPS KKFRSGSSFS RRAGSSGNSC ITYQPSVSGE HKAQVTTKAE
510 520 530 540 550
VEPGVHLGRP DVLPQTPPLE EISEGSPIPD PSFSPLVGET LQMLTTSTTL
560 562
EKLEVAESEF TH

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-5676 Collapse assay details

Data source: Panorama

Official Gene Symbol
PIP5K1A
Peptide Sequence
SVDSSGETTYK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
3
Peptide Start
68
Peptide End
78
CPTAC ID
non-CPTAC-5676
Peptide Molecular Mass
1,172.5197
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
SUM159 tryptic digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Q-Exactive
Internal Standard
25fmol
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
EASY-nLC 1000
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (1+) 1.5 1.6 5.1 1.5 1.5 6.8 2.1 2.2 8.5 15 15 15
y9 (1+) 1.1 1.1 2.4 2.4 3.9 4.2 2.6 4.1 4.8 15 15 15
y4 (1+) 2.3 1.9 4.4 2.3 2 5.8 3.3 2.8 7.3 15 15 15
sum 0.9 1.2 2 1.6 2.3 4 1.8 2.6 4.5 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments