PKMYT1, CPTAC-5677 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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Overview Data source: UniProt

Official Gene Symbol	Other Aliases
PKMYT1	MYT1

Sequence Length (AA)	Molecular Weight (Da)
499	54521

Protein Name
Membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MLERPPALAM	PMPTEGTPPP	LSGTPIPVPA	YFRHAEPGFS	LKRPRGLSRS
60	70	80	90	100
LPPPPPAKGS	IPISRLFPPR	TPGWHQLQPR	RVSFRGEASE	TLQSPGYDPS
110	120	130	140	150
RPESFFQQSF	QRLSRLGHGS	YGEVFKVRSK	EDGRLYAVKR	SMSPFRGPKD
160	170	180	190	200
RARKLAEVGS	HEKVGQHPCC	VRLEQAWEEG	GILYLQTELC	GPSLQQHCEA
210	220	230	240	250
WGASLPEAQV	WGYLRDTLLA	LAHLHSQGLV	HLDVKPANIF	LGPRGRCKLG
260	270	280	290	300
DFGLLVELGT	AGAGEVQEGD	PRYMAPELLQ	GSYGTAADVF	SLGLTILEVA
310	320	330	340	350
CNMELPHGGE	GWQQLRQGYL	PPEFTAGLSS	ELRSVLVMML	EPDPKLRATA
360	370	380	390	400
EALLALPVLR	QPRAWGVLWC	MAAEALSRGW	ALWQALLALL	CWLWHGLAHP
410	420	430	440	450
ASWLQPLGPP	ATPPGSPPCS	LLLDSSLSSN	WDDDSLGPSL	SPEAVLARTV
460	470	480	490	499
GSTSTPRSRC	TPRDALDLSD	INSEPPRGSF	PSFEPRNLLS	LFEDTLDPT

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for non-CPTAC-5677 Collapse assay details

Data source: Panorama

Official Gene Symbol: PKMYT1
Peptide Sequence: GSFPSFEPR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: 3
Peptide Start: 478
Peptide End: 486

CPTAC ID: non-CPTAC-5677
Peptide Molecular Mass: 1,022.4821
Species: Homo sapiens (Human)
Assay Type: Direct PRM
Matrix: SUM159 tryptic digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Q-Exactive
Internal Standard: 25fmol
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: EASY-nLC 1000
Column Packing: C18
Column Dimensions: 75 µm x 50 cm
Flow Rate: 300nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y5 (1+)	2.2	1.4	3.5	2.3	2	3.8	3.2	2.4	5.2	15	15	15
y4 (1+)	1.9	2.2	6.6	2.1	2.4	6	2.8	3.3	8.9	15	15	15
y6 (1+)	2.1	1.6	2.7	3.2	3.2	3	3.8	3.6	4	15	15	15
sum	1.8	1	2.5	2.2	2.2	2.7	2.8	2.4	3.7	15	15	15

PKMYT1

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for non-CPTAC-5677 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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PKMYT1

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for non-CPTAC-5677 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments