NEK7, CPTAC-5680 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
NEK7	N/A

Sequence Length (AA)	Molecular Weight (Da)
302	34551

Protein Name
Serine/threonine-protein kinase Nek7

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MDEQSQGMQG	PPVPQFQPQK	ALRPDMGYNT	LANFRIEKKI	GRGQFSEVYR
60	70	80	90	100
AACLLDGVPV	ALKKVQIFDL	MDAKARADCI	KEIDLLKQLN	HPNVIKYYAS
110	120	130	140	150
FIEDNELNIV	LELADAGDLS	RMIKHFKKQK	RLIPERTVWK	YFVQLCSALE
160	170	180	190	200
HMHSRRVMHR	DIKPANVFIT	ATGVVKLGDL	GLGRFFSSKT	TAAHSLVGTP
210	220	230	240	250
YYMSPERIHE	NGYNFKSDIW	SLGCLLYEMA	ALQSPFYGDK	MNLYSLCKKI
260	270	280	290	300
EQCDYPPLPS	DHYSEELRQL	VNMCINPDPE	KRPDVTYVYD	VAKRMHACTA
302
SS

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-1621 Collapse assay details

Data source: Panorama

Official Gene Symbol: NEK7
Peptide Sequence: VQIFDLMDAK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 65
Peptide End: 74

CPTAC ID: CPTAC-1621
Peptide Molecular Mass: 1,178.6005
Species: Homo sapiens (Human)
Assay Type: Direct PRM
Matrix: Ovarian cancer tumor tissue lysate
Submitting Laboratory: Pacific Northwest National Laboratory
Submitting Lab PI: Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: TSQ Vantage
Internal Standard: peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Waters nanoACQUITY (Part Number 176016000)
Column Packing: Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions: 100 um x 100 mm
Flow Rate: 0.5 uL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y6 (1+)	29.6	6.5	4.6	35.3	7.9	6.3	46.1	10.2	7.8	15	15	15
y7 (1+)	7.6	2.6	1.8	17.7	7.3	4.4	19.3	7.7	4.8	15	15	15
y8 (1+)	8	3.3	2.3	13.5	6.4	4	15.7	7.2	4.6	15	15	15
sum	5.4	2.9	1.7	13.3	6.6	4.1	14.4	7.2	4.4	15	15	15

Additional Resources and Comments

Resources

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View Peptide in Panorama

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Comments

Assay Details for non-CPTAC-5680 Collapse assay details

Data source: Panorama

Official Gene Symbol: NEK7
Peptide Modified Sequence: AAC[+57.021464]LLDGVPVALK
Modification Type: Carbamidomethyl (C)
Protein - Site of Modification: N/A
Peptide - Site of Modification: 3
Peptide Start: 51
Peptide End: 63

CPTAC ID: non-CPTAC-5680
Peptide Molecular Mass: 1,325.7377
Species: Homo sapiens (Human)
Assay Type: Direct PRM
Matrix: SUM159 tryptic digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend