STK3, CPTAC-5686 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
STK3	KRS1, MST2

Sequence Length (AA)	Molecular Weight (Da)
491	56301

Protein Name
Serine/threonine-protein kinase 3

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MEQPPAPKSK	LKKLSEDSLT	KQPEEVFDVL	EKLGEGSYGS	VFKAIHKESG
60	70	80	90	100
QVVAIKQVPV	ESDLQEIIKE	ISIMQQCDSP	YVVKYYGSYF	KNTDLWIVME
110	120	130	140	150
YCGAGSVSDI	IRLRNKTLIE	DEIATILKST	LKGLEYLHFM	RKIHRDIKAG
160	170	180	190	200
NILLNTEGHA	KLADFGVAGQ	LTDTMAKRNT	VIGTPFWMAP	EVIQEIGYNC
210	220	230	240	250
VADIWSLGIT	SIEMAEGKPP	YADIHPMRAI	FMIPTNPPPT	FRKPELWSDD
260	270	280	290	300
FTDFVKKCLV	KNPEQRATAT	QLLQHPFIKN	AKPVSILRDL	ITEAMEIKAK
310	320	330	340	350
RHEEQQRELE	EEEENSDEDE	LDSHTMVKTS	VESVGTMRAT	STMSEGAQTM
360	370	380	390	400
IEHNSTMLES	DLGTMVINSE	DEEEEDGTMK	RNATSPQVQR	PSFMDYFDKQ
410	420	430	440	450
DFKNKSHENC	NQNMHEPFPM	SKNVFPDNWK	VPQDGDFDFL	KNLSLEELQM
460	470	480	490	491
RLKALDPMME	REIEELRQRY	TAKRQPILDA	MDAKKRRQQN	F

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for non-CPTAC-5686 Collapse assay details

Data source: Panorama

Official Gene Symbol: STK3
Peptide Sequence: LSEDSLTK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: 3
Peptide Start: 14
Peptide End: 21

CPTAC ID: non-CPTAC-5686
Peptide Molecular Mass: 891.4549
Species: Homo sapiens (Human)
Assay Type: Direct PRM
Matrix: SUM159 tryptic digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Q-Exactive
Internal Standard: 25fmol
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: EASY-nLC 1000
Column Packing: C18
Column Dimensions: 75 µm x 50 cm
Flow Rate: 300nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y6 (1+)	2.7	2.2	6.2	2.2	2	8.1	3.5	3	10.2	15	15	15
y5 (1+)	1.4	2.5	11.8	2.1	2.7	11.6	2.5	3.7	16.5	15	15	15
y4 (1+)	2.3	2.2	12.5	2.1	3.2	8.1	3.1	3.9	14.9	15	15	15
sum	1.6	1.8	6.6	1.1	2.1	5.6	1.9	2.8	8.7	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

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Comments

Assay Details for non-CPTAC-2873 Collapse assay details

Data source: Panorama

Official Gene Symbol: STK3
Peptide Sequence: TLIEDEIATILK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 117
Peptide End: 128

CPTAC ID: non-CPTAC-2873
Peptide Molecular Mass: 1,357.7704
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend