PI4KA, CPTAC-5994 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
PI4KA	PIK4, PIK4CA

Sequence Length (AA)	Molecular Weight (Da)
2102	236830

Protein Name
Phosphatidylinositol 4-kinase alpha

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MAAAPARGGG	GGGGGGGGCS	GSGSSASRGF	YFNTVLSLAR	SLAVQRPASL
60	70	80	90	100
EKVQKLLCMC	PVDFHGIFQL	DERRRDAVIA	LGIFLIESDL	QHKDCVVPYL
110	120	130	140	150
LRLLKGLPKV	YWVEESTARK	GRGALPVAES	FSFCLVTLLS	DVAYRDPSLR
160	170	180	190	200
DEILEVLLQV	LHVLLGMCQA	LEIQDKEYLC	KYAIPCLIGI	SRAFGRYSNM
210	220	230	240	250
EESLLSKLFP	KIPPHSLRVL	EELEGVRRRS	FNDFRSILPS	NLLTVCQEGT
260	270	280	290	300
LKRKTSSVSS	ISQVSPERGM	PPPSSPGGSA	FHYFEASCLP	DGTALEPEYY
310	320	330	340	350
FSTISSSFSV	SPLFNGVTYK	EFNIPLEMLR	ELLNLVKKIV	EEAVLKSLDA
360	370	380	390	400
IVASVMEANP	SADLYYTSFS	DPLYLTMFKM	LRDTLYYMKD	LPTSFVKEIH
410	420	430	440	450
DFVLEQFNTS	QGELQKILHD	ADRIHNELSP	LKLRCQANAA	CVDLMVWAVK
460	470	480	490	500
DEQGAENLCI	KLSEKLQSKT	SSKVIIAHLP	LLICCLQGLG	RLCERFPVVV
510	520	530	540	550
HSVTPSLRDF	LVIPSPVLVK	LYKYHSQYHT	VAGNDIKISV	TNEHSESTLN
560	570	580	590	600
VMSGKKSQPS	MYEQLRDIAI	DNICRCLKAG	LTVDPVIVEA	FLASLSNRLY
610	620	630	640	650
ISQESDKDAH	LIPDHTIRAL	GHIAVALRDT	PKVMEPILQI	LQQKFCQPPS
660	670	680	690	700
PLDVLIIDQL	GCLVITGNQY	IYQEVWNLFQ	QISVKASSVV	YSATKDYKDH
710	720	730	740	750
GYRHCSLAVI	NALANIAANI	QDEHLVDELL	MNLLELFVQL	GLEGKRASER
760	770	780	790	800
ASEKGPALKA	SSSAGNLGVL	IPVIAVLTRR	LPPIKEAKPR	LQKLFRDFWL
810	820	830	840	850
YSVLMGFAVE	GSGLWPEEWY	EGVCEIATKS	PLLTFPSKEP	LRSVLQYNSA
860	870	880	890	900
MKNDTVTPAE	LSELRSTIIN	LLDPPPEVSA	LINKLDFAMS	TYLLSVYRLE
910	920	930	940	950
YMRVLRSTDP	DRFQVMFCYF	EDKAIQKDKS	GMMQCVIAVA	DKVFDAFLNM
960	970	980	990	1000
MADKAKTKEN	EEELERHAQF	LLVNFNHIHK	RIRRVADKYL	SGLVDKFPHL
1010	1020	1030	1040	1050
LWSGTVLKTM	LDILQTLSLS	LSADIHKDQP	YYDIPDAPYR	ITVPDTYEAR
1060	1070	1080	1090	1100
ESIVKDFAAR	CGMILQEAMK	WAPTVTKSHL	QEYLNKHQNW	VSGLSQHTGL
1110	1120	1130	1140	1150
AMATESILHF	AGYNKQNTTL	GATQLSERPA	CVKKDYSNFM	ASLNLRNRYA
1160	1170	1180	1190	1200
GEVYGMIRFS	GTTGQMSDLN	KMMVQDLHSA	LDRSHPQHYT	QAMFKLTAML
1210	1220	1230	1240	1250
ISSKDCDPQL	LHHLCWGPLR	MFNEHGMETA	LACWEWLLAG	KDGVEVPFMR
1260	1270	1280	1290	1300
EMAGAWHMTV	EQKFGLFSAE	IKEADPLAAS	EASQPKPCPP	EVTPHYIWID
1310	1320	1330	1340	1350
FLVQRFEIAK	YCSSDQVEIF	SSLLQRSMSL	NIGGAKGSMN	RHVAAIGPRF
1360	1370	1380	1390	1400
KLLTLGLSLL	HADVVPNATI	RNVLREKIYS	TAFDYFSCPP	KFPTQGEKRL
1410	1420	1430	1440	1450
REDISIMIKF	WTAMFSDKKY	LTASQLVPPD	NQDTRSNLDI	TVGSRQQATQ
1460	1470	1480	1490	1500
GWINTYPLSS	GMSTISKKSG	MSKKTNRGSQ	LHKYYMKRRT	LLLSLLATEI
1510	1520	1530	1540	1550
ERLITWYNPL	SAPELELDQA	GENSVANWRS	KYISLSEKQW	KDNVNLAWSI
1560	1570	1580	1590	1600
SPYLAVQLPA	RFKNTEAIGN	EVTRLVRLDP	GAVSDVPEAI	KFLVTWHTID
1610	1620	1630	1640	1650
ADAPELSHVL	CWAPTDPPTG	LSYFSSMYPP	HPLTAQYGVK	VLRSFPPDAI
1660	1670	1680	1690	1700
LFYIPQIVQA	LRYDKMGYVR	EYILWAASKS	QLLAHQFIWN	MKTNIYLDEE
1710	1720	1730	1740	1750
GHQKDPDIGD	LLDQLVEEIT	GSLSGPAKDF	YQREFDFFNK	ITNVSAIIKP
1760	1770	1780	1790	1800
YPKGDERKKA	CLSALSEVKV	QPGCYLPSNP	EAIVLDIDYK	SGTPMQSAAK
1810	1820	1830	1840	1850
APYLAKFKVK	RCGVSELEKE	GLRCRSDSED	ECSTQEADGQ	KISWQAAIFK
1860	1870	1880	1890	1900
VGDDCRQDML	ALQIIDLFKN	IFQLVGLDLF	VFPYRVVATA	PGCGVIECIP
1910	1920	1930	1940	1950
DCTSRDQLGR	QTDFGMYDYF	TRQYGDESTL	AFQQARYNFI	RSMAAYSLLL
1960	1970	1980	1990	2000
FLLQIKDRHN	GNIMLDKKGH	IIHIDFGFMF	ESSPGGNLGW	EPDIKLTDEM
2010	2020	2030	2040	2050
VMIMGGKMEA	TPFKWFMEMC	VRGYLAVRPY	MDAVVSLVTL	MLDTGLPCFR
2060	2070	2080	2090	2100
GQTIKLLKHR	FSPNMTEREA	ANFIMKVIQS	CFLSNRSRTY	DMIQYYQNDI
2102
PY

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below

All other points link out to UniProt

Assay Details for non-CPTAC-5993 Collapse assay details

Data source: Panorama

Official Gene Symbol: PI4KA
Peptide Sequence: LYISQESDK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 599
Peptide End: 607

CPTAC ID: non-CPTAC-5993
Peptide Molecular Mass: 1,081.5292
Species: Human
Assay Type: Direct PRM
Matrix: SUM159 tryptic digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Q-Exactive
Internal Standard: 25fmol
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: EASY-nLC 1000
Column Packing: C18
Column Dimensions: 75 µm x 50 cm
Flow Rate: 300nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y3 (1+)	5.1	2	1.6	5.8	3.9	3.5	7.7	4.4	3.8	15	15	15
y6 (1+)	1.3	1.4	1.6	4	2.2	3.5	4.2	2.6	3.8	15	15	15
y7 (1+)	2.5	1.2	1.6	5.1	3	4.5	5.7	3.2	4.8	15	15	15
sum	2	1.1	1.5	3.9	2.5	3.7	4.4	2.7	4	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

Comments

Assay Details for non-CPTAC-5994 Collapse assay details

Data source: Panorama

Official Gene Symbol: PI4KA
Peptide Sequence: SPLLTFPSK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 830
Peptide End: 838

CPTAC ID: non-CPTAC-5994
Peptide Molecular Mass: 988.5593
Species: Human
Assay Type: Direct PRM
Matrix: SUM159 tryptic digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend