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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

PKN3

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
PKN3 PKNBETA
Sequence Length (AA) Molecular Weight (Da)
889 99421
Protein Name
Serine/threonine-protein kinase N3
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MEEGAPRQPG PSQWPPEDEK EVIRRAIQKE LKIKEGVENL RRVATDRRHL
60 70 80 90 100
GHVQQLLRSS NRRLEQLHGE LRELHARILL PGPGPGPAEP VASGPRPWAE
110 120 130 140 150
QLRARHLEAL RRQLHVELKV KQGAENMTHT CASGTPKERK LLAAAQQMLR
160 170 180 190 200
DSQLKVALLR MKISSLEASG SPEPGPELLA EELQHRLHVE AAVAEGAKNV
210 220 230 240 250
VKLLSSRRTQ DRKALAEAQA QLQESSQKLD LLRLALEQLL EQLPPAHPLR
260 270 280 290 300
SRVTRELRAA VPGYPQPSGT PVKPTALTGT LQVRLLGCEQ LLTAVPGRSP
310 320 330 340 350
AAALASSPSE GWLRTKAKHQ RGRGELASEV LAVLKVDNRV VGQTGWGQVA
360 370 380 390 400
EQSWDQTFVI PLERARELEI GVHWRDWRQL CGVAFLRLED FLDNACHQLS
410 420 430 440 450
LSLVPQGLLF AQVTFCDPVI ERRPRLQRQE RIFSKRRGQD FLRASQMNLG
460 470 480 490 500
MAAWGRLVMN LLPPCSSPST ISPPKGCPRT PTTLREASDP ATPSNFLPKK
510 520 530 540 550
TPLGEEMTPP PKPPRLYLPQ EPTSEETPRT KRPHMEPRTR RGPSPPASPT
560 570 580 590 600
RKPPRLQDFR CLAVLGRGHF GKVLLVQFKG TGKYYAIKAL KKQEVLSRDE
610 620 630 640 650
IESLYCEKRI LEAVGCTGHP FLLSLLACFQ TSSHACFVTE FVPGGDLMMQ
660 670 680 690 700
IHEDVFPEPQ ARFYVACVVL GLQFLHEKKI IYRDLKLDNL LLDAQGFLKI
710 720 730 740 750
ADFGLCKEGI GFGDRTSTFC GTPEFLAPEV LTQEAYTRAV DWWGLGVLLY
760 770 780 790 800
EMLVGECPFP GDTEEEVFDC IVNMDAPYPG FLSVQGLEFI QKLLQKCPEK
810 820 830 840 850
RLGAGEQDAE EIKVQPFFRT TNWQALLART IQPPFVPTLC GPADLRYFEG
860 870 880 889
EFTGLPPALT PPAPHSLLTA RQQAAFRDFD FVSERFLEP

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-5995 Collapse assay details

Data source: Panorama

Official Gene Symbol
PKN3
Peptide Sequence
GELASEVLAVLK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
324
Peptide End
335
CPTAC ID
non-CPTAC-5995
Peptide Molecular Mass
1,227.7075
Species
Human
Assay Type
Direct PRM
Matrix
SUM159 tryptic digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Q-Exactive
Internal Standard
25fmol
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
EASY-nLC 1000
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y9 (1+) 4.5 3 3.8 5 3.3 5.2 6.7 4.5 6.4 15 15 15
y4 (1+) 4.1 3.9 3.4 5.4 3.7 5.6 6.8 5.4 6.6 15 15 15
y8 (1+) 5.2 2.8 3.8 6.8 3.7 6.1 8.6 4.6 7.2 15 15 15
sum 4.5 3.1 3.6 5.7 3.4 5.7 7.3 4.6 6.7 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments

Assay Details for non-CPTAC-5996 Collapse assay details

Data source: Panorama

Official Gene Symbol
PKN3
Peptide Sequence
VLLVQFK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
573
Peptide End
579
CPTAC ID
non-CPTAC-5996
Peptide Molecular Mass
845.5375
Species
Human
Assay Type
Direct PRM
Matrix
SUM159 tryptic digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Q-Exactive
Internal Standard
25fmol
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
EASY-nLC 1000
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300nL/min

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (1+) 5.4 7.5 5.8 5.8 8.5 6.1 7.9 11.3 8.4 15 15 15
y3 (1+) 5.7 9.6 7.2 5.7 10.2 7.6 8.1 14 10.5 15 15 15
y4 (1+) 5.8 9.5 6.3 6.2 10.1 7.2 8.5 13.9 9.6 15 15 15
sum 4.6 8.6 6.1 4.9 9.3 6.4 6.7 12.7 8.8 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments