TP53RK, CPTAC-5998 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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Overview Data source: UniProt

Official Gene Symbol	Other Aliases
TP53RK	C20orf64, PRPK

Sequence Length (AA)	Molecular Weight (Da)
253	28160

Protein Name
EKC/KEOPS complex subunit TP53RK

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MAAARATTPA	DGEEPAPEAE	ALAAARERSS	RFLSGLELVK	QGAEARVFRG
60	70	80	90	100
RFQGRAAVIK	HRFPKGYRHP	ALEARLGRRR	TVQEARALLR	CRRAGISAPV
110	120	130	140	150
VFFVDYASNC	LYMEEIEGSV	TVRDYIQSTM	ETEKTPQGLS	NLAKTIGQVL
160	170	180	190	200
ARMHDEDLIH	GDLTTSNMLL	KPPLEQLNIV	LIDFGLSFIS	ALPEDKGVDL
210	220	230	240	250
YVLEKAFLST	HPNTETVFEA	FLKSYSTSSK	KARPVLKKLD	EVRLRGRKRS
253
MVG

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for non-CPTAC-5997 Collapse assay details

Data source: Panorama

Official Gene Symbol: TP53RK
Peptide Sequence: FLSGLELVK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 32
Peptide End: 40

CPTAC ID: non-CPTAC-5997
Peptide Molecular Mass: 1,004.5906
Species: Human
Assay Type: Direct PRM
Matrix: SUM159 tryptic digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Q-Exactive
Internal Standard: 25fmol
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: EASY-nLC 1000
Column Packing: C18
Column Dimensions: 75 µm x 50 cm
Flow Rate: 300nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y6 (1+)	7.7	8.5	6.7	8.7	8.1	7.2	11.6	11.7	9.8	15	15	15
y3 (1+)	5.8	9.7	4.8	6.4	9.1	5.7	8.6	13.3	7.5	15	15	15
y7 (1+)	8.2	7.9	4.8	8	7.7	5.6	11.5	11	7.4	15	15	15
sum	7.5	8	4.7	7.5	7.6	5.5	10.6	11	7.2	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

Comments

Assay Details for non-CPTAC-5998 Collapse assay details

Data source: Panorama

Official Gene Symbol: TP53RK
Peptide Sequence: GVDLYVLEK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 197
Peptide End: 205

CPTAC ID: non-CPTAC-5998
Peptide Molecular Mass: 1,034.5648
Species: Human
Assay Type: Direct PRM
Matrix: SUM159 tryptic digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend