RIOK1, CPTAC-5999 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
RIOK1	RIO1

Sequence Length (AA)	Molecular Weight (Da)
568	65583

Protein Name
Serine/threonine-protein kinase RIO1

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MDYRRLLMSR	VVPGQFDDAD	SSDSENRDLK	TVKEKDDILF	EDLQDNVNEN
60	70	80	90	100
GEGEIEDEEE	EGYDDDDDDW	DWDEGVGKLA	KGYVWNGGSN	PQANRQTSDS
110	120	130	140	150
SSAKMSTPAD	KVLRKFENKI	NLDKLNVTDS	VINKVTEKSR	QKEADMYRIK
160	170	180	190	200
DKADRATVEQ	VLDPRTRMIL	FKMLTRGIIT	EINGCISTGK	EANVYHASTA
210	220	230	240	250
NGESRAIKIY	KTSILVFKDR	DKYVSGEFRF	RHGYCKGNPR	KMVKTWAEKE
260	270	280	290	300
MRNLIRLNTA	EIPCPEPIML	RSHVLVMSFI	GKDDMPAPLL	KNVQLSESKA
310	320	330	340	350
RELYLQVIQY	MRRMYQDARL	VHADLSEFNM	LYHGGGVYII	DVSQSVEHDH
360	370	380	390	400
PHALEFLRKD	CANVNDFFMR	HSVAVMTVRE	LFEFVTDPSI	THENMDAYLS
410	420	430	440	450
KAMEIASQRT	KEERSSQDHV	DEEVFKRAYI	PRTLNEVKNY	ERDMDIIMKL
460	470	480	490	500
KEEDMAMNAQ	QDNILYQTVT	GLKKDLSGVQ	KVPALLENQV	EERTCSDSED
510	520	530	540	550
IGSSECSDTD	SEEQGDHARP	KKHTTDPDID	KKERKKMVKE	AQREKRKNKI
560	568
PKHVKKRKEK	TAKTKKGK

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for non-CPTAC-5999 Collapse assay details

Data source: Panorama

Official Gene Symbol: RIOK1
Peptide Sequence: LNVTDSVINK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 125
Peptide End: 134

CPTAC ID: non-CPTAC-5999
Peptide Molecular Mass: 1,101.6030
Species: Human
Assay Type: Direct PRM
Matrix: SUM159 tryptic digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Q-Exactive
Internal Standard: 25fmol
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: EASY-nLC 1000
Column Packing: C18
Column Dimensions: 75 µm x 50 cm
Flow Rate: 300nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y6 (1+)	2.9	2.3	1.2	3.4	1.8	2.4	4.5	2.9	2.7	15	15	15
y3 (1+)	2.6	1.1	1.5	3	1.9	2.2	4	2.2	2.7	15	15	15
y7 (1+)	1.4	1.1	1.6	2.9	1.4	2.4	3.2	1.8	2.9	15	15	15
sum	1.3	1.1	1.3	2.3	1.5	2.2	2.6	1.9	2.6	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

Comments

Assay Details for non-CPTAC-6000 Collapse assay details

Data source: Panorama

Official Gene Symbol: RIOK1
Peptide Sequence: VPALLENQVEER
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 482
Peptide End: 493

CPTAC ID: non-CPTAC-6000
Peptide Molecular Mass: 1,395.7358
Species: Human
Assay Type: Direct PRM
Matrix: SUM159 tryptic digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend