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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

ULK4

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
ULK4 N/A
Sequence Length (AA) Molecular Weight (Da)
1275 142442
Protein Name
Serine/threonine-protein kinase ULK4
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MENFILYEEI GRGSKTVVYK GRRKGTINFV AILCTDKCKR PEITNWVRLT
60 70 80 90 100
REIKHKNIVT FHEWYETSNH LWLVVELCTG GSLKTVIAQD ENLPEDVVRE
110 120 130 140 150
FGIDLISGLH HLHKLGILFC DISPRKILLE GPGTLKFSNF CLAKVEGENL
160 170 180 190 200
EEFFALVAAE EGGGDNGENV LKKSMKSRVK GSPVYTAPEV VRGADFSISS
210 220 230 240 250
DLWSLGCLLY EMFSGKPPFF SESISELTEK ILCEDPLPPI PKDSSRPKAS
260 270 280 290 300
SDFINLLDGL LQRDPQKRLT WTRLLQHSFW KKAFAGADQE SSVEDLSLSR
310 320 330 340 350
NTMECSGPQD SKELLQNSQS RQAKGHKSGQ PLGHSFRLEN PTEFRPKSTL
360 370 380 390 400
EGQLNESMFL LSSRPTPRTS TAVEVSPGED MTHCSPQKTS PLTKITSGHL
410 420 430 440 450
SQQDLESQMR ELIYTDSDLV VTPIIDNPKI MKQPPVKFDA KILHLPTYSV
460 470 480 490 500
DKLLFLKDQD WNDFLQQVCS QIDSTEKSMG ASRAKLNLLC YLCVVAGHQE
510 520 530 540 550
VATRLLHSPL FQLLIQHLRI APNWDIRAKV AHVIGLLASH TAELQENTPV
560 570 580 590 600
VEAIVLLTEL IRENFRNSKL KQCLLPTLGE LIYLVATQEE KKKNPRECWA
610 620 630 640 650
VPLAAYTVLM RCLREGEERV VNHMAAKIIE NVCTTFSAQS QGFITGEIGP
660 670 680 690 700
ILWYLFRHST ADSLRITAVS ALCRITRHSP TAFQNVIEKV GLNSVINSLA
710 720 730 740 750
SAICKVQQYM LTLFAAMLSC GIHLQRLIQE KGFVSTIIRL LDSPSTCIRA
760 770 780 790 800
KAFLVLLYIL IYNREMLLLS CQARLVMYIE RDSRKTTPGK EQQSGNEYLS
810 820 830 840 850
KCLDLLICHI VQELPRILGD ILNSLANVSG RKHPSTVQVK QLKLCLPLMP
860 870 880 890 900
VVLHLVTSQV FRPQVVTEEF LFSYGTILSH IKSVDSGETN IDGAIGLTAS
910 920 930 940 950
EEFIKITLSA FEAIIQYPIL LKDYRSTVVD YILPPLVSLV QSQNVEWRLF
960 970 980 990 1000
SLRLLSETTS LLVNQEFGDG KEKASVDSDS NLLALIRDVL LPQYEHILLE
1010 1020 1030 1040 1050
PDPVPAYALK LLVAMTEHNP TFTRLVEESK LIPLIFEVTL EHQESILGNT
1060 1070 1080 1090 1100
MQSVIALLSN LVACKDSNME LLYEQGLVSH ICNLLTETAT LCLDVDNKNN
1110 1120 1130 1140 1150
NEMAAPLLFS LLDILHSMLT YTSGIVRLAL QAQKSGSGED PQAAEDLLLL
1160 1170 1180 1190 1200
NRPLTDLISL LIPLLPNEDP EIFDVSSKCL SILVQLYGGE NPDSLSPENV
1210 1220 1230 1240 1250
EIFAHLLTSK EDPKEQKLLL RILRRMITSN EKHLESLKNA GSLLRALERL
1260 1270 1275
APGSGSFADS AVAPLALEIL QAVGH

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt


Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-6009 Collapse assay details

Data source: Panorama

Official Gene Symbol
ULK4
Peptide Sequence
ASVDSDSNLLALIR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
974
Peptide End
987
CPTAC ID
non-CPTAC-6009
Peptide Molecular Mass
1,472.7835
Species
Human
Assay Type
Direct PRM
Matrix
SUM159 tryptic digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Q-Exactive
Internal Standard
25fmol
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
EASY-nLC 1000
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y11 (1+) 11.8 3.5 3 14.5 4.7 3.5 18.7 5.9 4.6 15 15 15
y10 (1+) 13.6 3.8 3.5 14.3 4.8 3.6 19.7 6.1 5 15 15 15
y4 (1+) 12.5 3.6 3.8 13.9 4.5 4.3 18.7 5.8 5.7 15 15 15
sum 12.4 3.4 3.1 14.1 4.5 3.6 18.8 5.6 4.8 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments

Assay Details for non-CPTAC-6008 Collapse assay details

Data source: Panorama

Official Gene Symbol
ULK4
Peptide Sequence
ILLEGPGTLK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
127
Peptide End
136
CPTAC ID
non-CPTAC-6008
Peptide Molecular Mass
1,039.6277
Species
Human
Assay Type
Direct PRM
Matrix
SUM159 tryptic digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Q-Exactive
Internal Standard
25fmol
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
EASY-nLC 1000
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300nL/min

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 4.2 5 2.9 6.4 4.3 3 7.7 6.6 4.2 15 15 15
y5 (1+) 5.2 5.2 4.1 6.8 3.6 4.2 8.6 6.3 5.9 15 15 15
y8 (1+) 2.8 6.5 3.6 6.5 5.3 3.8 7.1 8.4 5.2 15 15 15
sum 2.9 5.1 3.1 5.6 4.1 3.3 6.3 6.5 4.5 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments