VRK3, CPTAC-6010 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
VRK3	N/A

Sequence Length (AA)	Molecular Weight (Da)
474	52881

Protein Name
Inactive serine/threonine-protein kinase VRK3

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MISFCPDCGK	SIQAAFKFCP	YCGNSLPVEE	HVGSQTFVNP	HVSSFQGSKR
60	70	80	90	100
GLNSSFETSP	KKVKWSSTVT	SPRLSLFSDG	DSSESEDTLS	SSERSKGSGS
110	120	130	140	150
RPPTPKSSPQ	KTRKSPQVTR	GSPQKTSCSP	QKTRQSPQTL	KRSRVTTSLE
160	170	180	190	200
ALPTGTVLTD	KSGRQWKLKS	FQTRDNQGIL	YEAAPTSTLT	CDSGPQKQKF
210	220	230	240	250
SLKLDAKDGR	LFNEQNFFQR	AAKPLQVNKW	KKLYSTPLLA	IPTCMGFGVH
260	270	280	290	300
QDKYRFLVLP	SLGRSLQSAL	DVSPKHVLSE	RSVLQVACRL	LDALEFLHEN
310	320	330	340	350
EYVHGNVTAE	NIFVDPEDQS	QVTLAGYGFA	FRYCPSGKHV	AYVEGSRSPH
360	370	380	390	400
EGDLEFISMD	LHKGCGPSRR	SDLQSLGYCM	LKWLYGFLPW	TNCLPNTEDI
410	420	430	440	450
MKQKQKFVDK	PGPFVGPCGH	WIRPSETLQK	YLKVVMALTY	EEKPPYAMLR
460	470	474
NNLEALLQDL	RVSPYDPIGL	PMVP

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for non-CPTAC-6011 Collapse assay details

Data source: Panorama

Official Gene Symbol: VRK3
Peptide Sequence: LFNEQNFFQR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 211
Peptide End: 220

CPTAC ID: non-CPTAC-6011
Peptide Molecular Mass: 1,341.6466
Species: Human
Assay Type: Direct PRM
Matrix: SUM159 tryptic digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Q-Exactive
Internal Standard: 25fmol
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: EASY-nLC 1000
Column Packing: C18
Column Dimensions: 75 µm x 50 cm
Flow Rate: 300nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y6 (1+)	3.6	3.6	6	5.6	3.5	7.3	6.7	5	9.4	15	15	15
y5 (1+)	6.7	3.2	4.8	6.2	3.5	6.8	9.1	4.7	8.3	15	15	15
y8 (1+)	5.6	3.6	6.1	6.4	3.8	7	8.5	5.2	9.3	15	15	15
sum	4.8	3.1	5.5	5.6	3.2	6.8	7.4	4.5	8.7	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

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Comments

Assay Details for non-CPTAC-6010 Collapse assay details

Data source: Panorama

Official Gene Symbol: VRK3
Peptide Sequence: VTTSLEALPTGTVLTDK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 145
Peptide End: 161

CPTAC ID: non-CPTAC-6010
Peptide Molecular Mass: 1,744.9458
Species: Human
Assay Type: Direct PRM
Matrix: SUM159 tryptic digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend