BRSK2, CPTAC-6015 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
BRSK2	C11orf7, PEN11B, SADA, STK29

Sequence Length (AA)	Molecular Weight (Da)
736	81633

Protein Name
Serine/threonine-protein kinase BRSK2

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MTSTGKDGGA	QHAQYVGPYR	LEKTLGKGQT	GLVKLGVHCV	TCQKVAIKIV
60	70	80	90	100
NREKLSESVL	MKVEREIAIL	KLIEHPHVLK	LHDVYENKKY	LYLVLEHVSG
110	120	130	140	150
GELFDYLVKK	GRLTPKEARK	FFRQIISALD	FCHSHSICHR	DLKPENLLLD
160	170	180	190	200
EKNNIRIADF	GMASLQVGDS	LLETSCGSPH	YACPEVIRGE	KYDGRKADVW
210	220	230	240	250
SCGVILFALL	VGALPFDDDN	LRQLLEKVKR	GVFHMPHFIP	PDCQSLLRGM
260	270	280	290	300
IEVDAARRLT	LEHIQKHIWY	IGGKNEPEPE	QPIPRKVQIR	SLPSLEDIDP
310	320	330	340	350
DVLDSMHSLG	CFRDRNKLLQ	DLLSEEENQE	KMIYFLLLDR	KERYPSQEDE
360	370	380	390	400
DLPPRNEIDP	PRKRVDSPML	NRHGKRRPER	KSMEVLSVTD	GGSPVPARRA
410	420	430	440	450
IEMAQHGQRS	RSISGASSGL	STSPLSSPRV	TPHPSPRGSP	LPTPKGTPVH
460	470	480	490	500
TPKESPAGTP	NPTPPSSPSV	GGVPWRARLN	SIKNSFLGSP	RFHRRKLQVP
510	520	530	540	550
TPEEMSNLTP	ESSPELAKKS	WFGNFISLEK	EEQIFVVIKD	KPLSSIKADI
560	570	580	590	600
VHAFLSIPSL	SHSVISQTSF	RAEYKATGGP	AVFQKPVKFQ	VDITYTEGGE
610	620	630	640	650
AQKENGIYSV	TFTLLSGPSR	RFKRVVETIQ	AQLLSTHDPP	AAQHLSDTTN
660	670	680	690	700
CMEMMTGRLS	KCGSPLSNFF	DVIKQLFSDE	KNGQAAQAPS	TPAKRSAHGP
710	720	730	736
LGDSAAAGPG	PGGDAEYPTG	KDTAKMGPPT	ARREQP

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for non-CPTAC-6015 Collapse assay details

Data source: Panorama

Official Gene Symbol: BRSK2
Peptide Sequence: EEQIFVVIK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 531
Peptide End: 539

CPTAC ID: non-CPTAC-6015
Peptide Molecular Mass: 1,103.6227
Species: Human
Assay Type: Direct PRM
Matrix: SUM159 tryptic digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Q-Exactive
Internal Standard: 25fmol
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: EASY-nLC 1000
Column Packing: C18
Column Dimensions: 75 µm x 50 cm
Flow Rate: 300nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y5 (1+)	5.2	4.8	3.9	5.2	7.6	4	7.4	9	5.6	15	15	15
y3 (1+)	4.5	5.6	3.9	5.5	8	3.6	7.1	9.8	5.3	15	15	15
y4 (1+)	5.1	5.8	3.9	6.2	7.1	3.5	8	9.2	5.2	15	15	15
sum	4.5	5.2	3.6	4.9	7.4	3.4	6.7	9	5	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

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Comments

Assay Details for non-CPTAC-6014 Collapse assay details

Data source: Panorama

Official Gene Symbol: BRSK2
Peptide Sequence: LLQDLLSEEENQEK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 318
Peptide End: 331

CPTAC ID: non-CPTAC-6014
Peptide Molecular Mass: 1,686.8312
Species: Human
Assay Type: Direct PRM
Matrix: SUM159 tryptic digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend