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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

CDK11B

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
CDK11B CDC2L1, CDK11, PITSLREA, PK58
Sequence Length (AA) Molecular Weight (Da)
795 92620
Protein Name
Cyclin-dependent kinase 11B
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MGDEKDSWKV KTLDEILQEK KRRKEQEEKA EIKRLKNSDD RDSKRDSLEE
60 70 80 90 100
GELRDHRMEI TIRNSPYRRE DSMEDRGEED DSLAIKPPQQ MSRKEKAHHR
110 120 130 140 150
KDEKRKEKRR HRSHSAEGGK HARVKEKERE HERRKRHREE QDKARREWER
160 170 180 190 200
QKRREMAREH SRRERDRLEQ LERKRERERK MREQQKEQRE QKERERRAEE
210 220 230 240 250
RRKEREARRE VSAHHRTMRE DYSDKVKASH WSRSPPRPPR ERFELGDGRK
260 270 280 290 300
PGEARPAPAQ KPAQLKEEKM EERDLLSDLQ DISDSERKTS SAESSSAESG
310 320 330 340 350
SGSEEEEEEE EEEEEEGSTS EESEEEEEEE EEEEEETGSN SEEASEQSAE
360 370 380 390 400
EVSEEEMSED EERENENHLL VVPESRFDRD SGESEEAEEE VGEGTPQSSA
410 420 430 440 450
LTEGDYVPDS PALSPIELKQ ELPKYLPALQ GCRSVEEFQC LNRIEEGTYG
460 470 480 490 500
VVYRAKDKKT DEIVALKRLK MEKEKEGFPI TSLREINTIL KAQHPNIVTV
510 520 530 540 550
REIVVGSNMD KIYIVMNYVE HDLKSLMETM KQPFLPGEVK TLMIQLLRGV
560 570 580 590 600
KHLHDNWILH RDLKTSNLLL SHAGILKVGD FGLAREYGSP LKAYTPVVVT
610 620 630 640 650
LWYRAPELLL GAKEYSTAVD MWSVGCIFGE LLTQKPLFPG KSEIDQINKV
660 670 680 690 700
FKDLGTPSEK IWPGYSELPA VKKMTFSEHP YNNLRKRFGA LLSDQGFDLM
710 720 730 740 750
NKFLTYFPGR RISAEDGLKH EYFRETPLPI DPSMFPTWPA KSEQQRVKRG
760 770 780 790 795
TSPRPPEGGL GYSQLGDDDL KETGFHLTTT NQGASAAGPG FSLKF

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-6016 Collapse assay details

Data source: Panorama

Official Gene Symbol
CDK11B
Peptide Sequence
DLGTPSEK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
653
Peptide End
660
CPTAC ID
non-CPTAC-6016
Peptide Molecular Mass
845.4131
Species
Human
Assay Type
Direct PRM
Matrix
SUM159 tryptic digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Q-Exactive
Internal Standard
25fmol
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
EASY-nLC 1000
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 3.8 2.3 2.5 7.2 2.6 3.1 8.1 3.5 4 15 15 15
b3 (1+) 6.2 4.2 1.4 15.2 5.6 3 16.4 7 3.3 15 15 15
y4 (1+) 2.7 1.3 1.1 5.9 2.1 2.5 6.5 2.5 2.7 15 15 15
sum 2.9 1 0.9 6.6 1.8 2.4 7.2 2.1 2.6 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments