CDK19, CPTAC-6017 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

Please include the following statement when referencing the CPTAC Assay Portal

We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
CDK19	CDC2L6, CDK11, KIAA1028

Sequence Length (AA)	Molecular Weight (Da)
502	56802

Protein Name
Cyclin-dependent kinase 19

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MDYDFKAKLA	AERERVEDLF	EYEGCKVGRG	TYGHVYKARR	KDGKDEKEYA
60	70	80	90	100
LKQIEGTGIS	MSACREIALL	RELKHPNVIA	LQKVFLSHSD	RKVWLLFDYA
110	120	130	140	150
EHDLWHIIKF	HRASKANKKP	MQLPRSMVKS	LLYQILDGIH	YLHANWVLHR
160	170	180	190	200
DLKPANILVM	GEGPERGRVK	IADMGFARLF	NSPLKPLADL	DPVVVTFWYR
210	220	230	240	250
APELLLGARH	YTKAIDIWAI	GCIFAELLTS	EPIFHCRQED	IKTSNPFHHD
260	270	280	290	300
QLDRIFSVMG	FPADKDWEDI	RKMPEYPTLQ	KDFRRTTYAN	SSLIKYMEKH
310	320	330	340	350
KVKPDSKVFL	LLQKLLTMDP	TKRITSEQAL	QDPYFQEDPL	PTLDVFAGCQ
360	370	380	390	400
IPYPKREFLN	EDDPEEKGDK	NQQQQQNQHQ	QPTAPPQQAA	APPQAPPPQQ
410	420	430	440	450
NSTQTNGTAG	GAGAGVGGTG	AGLQHSQDSS	LNQVPPNKKP	RLGPSGANSG
460	470	480	490	500
GPVMPSDYQH	SSSRLNYQSS	VQGSSQSQST	LGYSSSSQQS	SQYHPSHQAH
502
RY

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node

to view detailed assay information below

All other points link out to UniProt

Assay Details for non-CPTAC-6017 Collapse assay details

Data source: Panorama

Official Gene Symbol: CDK19
Peptide Sequence: VFLLLQK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 308
Peptide End: 314

CPTAC ID: non-CPTAC-6017
Peptide Molecular Mass: 859.5531
Species: Human
Assay Type: Direct PRM
Matrix: SUM159 tryptic digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Q-Exactive
Internal Standard: 25fmol
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: EASY-nLC 1000
Column Packing: C18
Column Dimensions: 75 µm x 50 cm
Flow Rate: 300nL/min

Chromatograms

Data source: Panorama

view larger image

Response Curves

Data source: Panorama

view larger image

Retrieving Data

Repeatability

Data source: Panorama

view larger image

	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y5 (1+)	5	5	4.9	6.2	7.9	7.2	8	9.3	8.7	15	15	15
y4 (1+)	6.1	5.1	4.3	6.6	7.8	6.4	9	9.3	7.7	15	15	15
y3 (1+)	5.2	5.9	4.7	7.2	9.3	6.3	8.9	11	7.9	15	15	15
sum	4.8	5	4.5	6.1	8.3	6.4	7.8	9.7	7.8	15	15	15

CDK19

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for non-CPTAC-6017 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

Search form

CDK19

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for non-CPTAC-6017 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments