CDKL1, CPTAC-6019 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
CDKL1	N/A

Sequence Length (AA)	Molecular Weight (Da)
358	41803

Protein Name
Cyclin-dependent kinase-like 1

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MMEKYEKIGK	IGEGSYGVVF	KCRNRDTGQI	VAIKKFLESE	DDPVIKKIAL
60	70	80	90	100
REIRMLKQLK	HPNLVNLLEV	FRRKRRLHLV	FEYCDHTVLH	ELDRYQRGVP
110	120	130	140	150
EHLVKSITWQ	TLQAVNFCHK	HNCIHRDVKP	ENILITKHSV	IKLCDFGFAR
160	170	180	190	200
LLAGPSDYYT	DYVATRWYRS	PELLVGDTQY	GPPVDVWAIG	CVFAELLSGV
210	220	230	240	250
PLWPGKSDVD	QLYLIRKTLG	DLIPRHQQVF	STNQYFSGVK	IPDPEDMEPL
260	270	280	290	300
ELKFPNISYP	ALGLLKGCLH	MDPTQRLTCE	QLLHHPYFEN	IREIEDLAKE
310	320	330	340	350
HNKPTRKTLR	KSRKHHCFTE	TSKLQYLPQL	TGSSILPALD	NKKYYCDTKK
358
LNYRFPNI

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for non-CPTAC-6019 Collapse assay details

Data source: Panorama

Official Gene Symbol: CDKL1
Peptide Sequence: FLESEDDPVIK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 36
Peptide End: 46

CPTAC ID: non-CPTAC-6019
Peptide Molecular Mass: 1,290.6343
Species: Human
Assay Type: Direct PRM
Matrix: SUM159 tryptic digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Q-Exactive
Internal Standard: 25fmol
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: EASY-nLC 1000
Column Packing: C18
Column Dimensions: 75 µm x 50 cm
Flow Rate: 300nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y9 (1+)	4.4	2.4	2	4.9	3.9	2.4	6.6	4.6	3.1	15	15	15
y8 (1+)	3.9	2.8	1.6	4.7	4.5	2.7	6.1	5.3	3.1	15	15	15
y4 (1+)	2.4	2.2	0.9	2.4	4.5	3.1	3.4	5	3.2	15	15	15
sum	2	2.1	0.9	1.9	4.2	2.5	2.8	4.7	2.7	15	15	15

Additional Resources and Comments

Resources

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View Peptide in Panorama

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Comments

Assay Details for non-CPTAC-6018 Collapse assay details

Data source: Panorama

Official Gene Symbol: CDKL1
Peptide Sequence: IGEGSYGVVFK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 11
Peptide End: 21

CPTAC ID: non-CPTAC-6018
Peptide Molecular Mass: 1,154.5972
Species: Human
Assay Type: Direct PRM
Matrix: SUM159 tryptic digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend