CLK3, CPTAC-6020 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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Overview Data source: UniProt

Official Gene Symbol	Other Aliases
CLK3	N/A

Sequence Length (AA)	Molecular Weight (Da)
638	73515

Protein Name
Dual specificity protein kinase CLK3

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MPVLSARRRE	LADHAGSGRR	SGPSPTARSG	PHLSALRAQP	ARAAHLSGRG
60	70	80	90	100
TYVRRDTAGG	GPGQARPLGP	PGTSLLGRGA	RRSGEGWCPG	AFESGARAAR
110	120	130	140	150
PPSRVEPRLA	TAASREGAGL	PRAEVAAGSG	RGARSGEWGL	AAAGAWETMH
160	170	180	190	200
HCKRYRSPEP	DPYLSYRWKR	RRSYSREHEG	RLRYPSRREP	PPRRSRSRSH
210	220	230	240	250
DRLPYQRRYR	ERRDSDTYRC	EERSPSFGED	YYGPSRSRHR	RRSRERGPYR
260	270	280	290	300
TRKHAHHCHK	RRTRSCSSAS	SRSQQSSKRS	SRSVEDDKEG	HLVCRIGDWL
310	320	330	340	350
QERYEIVGNL	GEGTFGKVVE	CLDHARGKSQ	VALKIIRNVG	KYREAARLEI
360	370	380	390	400
NVLKKIKEKD	KENKFLCVLM	SDWFNFHGHM	CIAFELLGKN	TFEFLKENNF
410	420	430	440	450
QPYPLPHVRH	MAYQLCHALR	FLHENQLTHT	DLKPENILFV	NSEFETLYNE
460	470	480	490	500
HKSCEEKSVK	NTSIRVADFG	SATFDHEHHT	TIVATRHYRP	PEVILELGWA
510	520	530	540	550
QPCDVWSIGC	ILFEYYRGFT	LFQTHENREH	LVMMEKILGP	IPSHMIHRTR
560	570	580	590	600
KQKYFYKGGL	VWDENSSDGR	YVKENCKPLK	SYMLQDSLEH	VQLFDLMRRM
610	620	630	638
LEFDPAQRIT	LAEALLHPFF	AGLTPEERSF	HTSRNPSR

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for non-CPTAC-6021 Collapse assay details

Data source: Panorama

Official Gene Symbol: CLK3
Peptide Sequence: IGDWLQER
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 296
Peptide End: 303

CPTAC ID: non-CPTAC-6021
Peptide Molecular Mass: 1,015.5087
Species: Human
Assay Type: Direct PRM
Matrix: SUM159 tryptic digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Q-Exactive
Internal Standard: 25fmol
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: EASY-nLC 1000
Column Packing: C18
Column Dimensions: 75 µm x 50 cm
Flow Rate: 300nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y5 (1+)	3.5	2.1	2.4	5	2.7	3.5	6.1	3.4	4.2	15	15	15
y4 (1+)	4.6	2.7	2.5	5	3.1	3.5	6.8	4.1	4.3	15	15	15
y3 (1+)	6.2	2.2	1.9	6.8	3.4	3.3	9.2	4	3.8	15	15	15
sum	4.3	1.2	1.8	4.1	2.5	3.2	5.9	2.8	3.7	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

Comments

Assay Details for non-CPTAC-6020 Collapse assay details

Data source: Panorama

Official Gene Symbol: CLK3
Peptide Sequence: SPSFGEDYYGPSR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 224
Peptide End: 236

CPTAC ID: non-CPTAC-6020
Peptide Molecular Mass: 1,460.6208
Species: Human
Assay Type: Direct PRM
Matrix: SUM159 tryptic digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend