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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
LMTK2 AATYK2, BREK, KIAA1079, KPI2, LMR2
Sequence Length (AA) Molecular Weight (Da)
1503 164900
Protein Name
Serine/threonine-protein kinase LMTK2
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MPGPPALRRR LLLLLLVLLI AGSAGAAPLP QTGAGEAPPA AEVSSSFVIL
60 70 80 90 100
CVCSLIILIV LIANCVSCCK DPEIDFKEFE DNFDDEIDFT PPAEDTPSVQ
110 120 130 140 150
SPAEVFTLSV PNISLPAPSQ FQPSVEGLKS QVARHSLNYI QEIGNGWFGK
160 170 180 190 200
VLLGEIYTGT SVARVIVKEL KASANPKEQD TFLKNGEPYY ILQHPNILQC
210 220 230 240 250
VGQCVEAIPY LLVFEFCDLG DLKAYLRSEQ EHMRGDSQTM LLQRMACEVA
260 270 280 290 300
AGLAAMHKLH FLHSDLALRN CFLTSDLNVK VGDYGIGFSR YKEDYIETDD
310 320 330 340 350
KKVFPLRWTA PELVTSFQDR LLTADQTKYS NIWSLGVTLW ELFDNAAQPY
360 370 380 390 400
SNLSNLDVLN QVIRERDTKL PKPQLEQPYS DRWYEVLQFC WLSPEKRPAA
410 420 430 440 450
EDVHRLLTYL RLQSQRDSEV DFEQQWNALK PNTNSRDSSN NAAFPILDHF
460 470 480 490 500
ARDRLGREME EVLTVTETSQ GLSFEYVWEA AKHDHFDERS RGHLDEGLSY
510 520 530 540 550
TSIFYPVEVF ESSLSDPGPG KQDDSGQDVP LRVPGVVPVF DAHNLSVGSD
560 570 580 590 600
YYIQLEEKSG SNLELDYPPA LLTTDMDNPE RTGPELSQLT ALRSVELEES
610 620 630 640 650
STDEDFFQSS TDPKDSSLPG DLHVTSGPES PFNNIFNDVD KSEDLPSHQK
660 670 680 690 700
IFDLMELNGV QADFKPATLS SSLDNPKESV ITGHFEKEKP RKIFDSEPLC
710 720 730 740 750
LSDNLMHQDN FDPLNVQELS ENFLFLQEKN LLKGSLSSKE HINDLQTELK
760 770 780 790 800
NAGFTEAMLE TSCRNSLDTE LQFAENKPGL SLLQENVSTK GDDTDVMLTG
810 820 830 840 850
DTLSTSLQSS PEVQVPPTSF ETEETPRRVP PDSLPTQGET QPTCLDVIVP
860 870 880 890 900
EDCLHQDISP DAVTVPVEIL STDARTHSLD NRSQDSPGES EETLRLTESD
910 920 930 940 950
SVLADDILAS RVSVGSSLPE LGQELHNKPF SEDHHSHRRL EKNLEAVETL
960 970 980 990 1000
NQLNSKDAAK EAGLVSALSS DSTSQDSLLE DSLSAPFPAS EPSLETPDSL
1010 1020 1030 1040 1050
ESVDVHEALL DSLGSHTPQK LVPPDKPADS GYETENLESP EWTLHPAPEG
1060 1070 1080 1090 1100
TADSEPATTG DGGHSGLPPN PVIVISDAGD GHRGTEVTPE TFTAGSQGSY
1110 1120 1130 1140 1150
RDSAYFSDND SEPEKRSEEV PGTSPSALVL VQEQPLPEPV LPEQSPAAQD
1160 1170 1180 1190 1200
SCLEARKSQP DESCLSALHN SSDLELRATP EPAQTGVPQQ VHPTEDEASS
1210 1220 1230 1240 1250
PWSVLNAELS SGDDFETQDD RPCTLASTGT NTNELLAYTN SALDKSLSSH
1260 1270 1280 1290 1300
SEGPKLKEPD IEGKYLGKLG VSGMLDLSED GMDADEEDEN SDDSDEDLRA
1310 1320 1330 1340 1350
FNLHSLSSES EDETEHPVPI ILSNEDGRHL RSLLKPTAAN APDPLPEDWK
1360 1370 1380 1390 1400
KEKKAVTFFD DVTVYLFDQE TPTKELGPCG GEACGPDLSG PAPASGSPYL
1410 1420 1430 1440 1450
SRCINSESST DEEGGGFEWD DDFSPDPFMS KTTSNLLSSK PSLQTSKYFS
1460 1470 1480 1490 1500
PPPPARSTEQ SWPHSAPYSR FSISPANIAS FSLTHLTDSD IEQGGSSEDG
1503
EKD

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-6031 Collapse assay details

Data source: Panorama

Official Gene Symbol
LMTK2
Peptide Sequence
VGDYGIGFSR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
281
Peptide End
290
CPTAC ID
non-CPTAC-6031
Peptide Molecular Mass
1,069.5193
Species
Human
Assay Type
Direct PRM
Matrix
SUM159 tryptic digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Q-Exactive
Internal Standard
25fmol
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
EASY-nLC 1000
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 2.9 2.7 2 4.9 2.4 2.7 5.7 3.6 3.4 15 15 15
y6 (1+) 3.7 1.6 2.1 5.2 1.9 3.5 6.4 2.5 4.1 15 15 15
y4 (1+) 2.1 2.2 2.2 3.4 2.5 4.4 4 3.3 4.9 15 15 15
sum 2 1.8 0.9 3.9 1.9 2.9 4.4 2.6 3 15 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-6030 Collapse assay details

Data source: Panorama

Official Gene Symbol
LMTK2
Peptide Sequence
VLLGEIYTGTSVAR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
151
Peptide End
164
CPTAC ID
non-CPTAC-6030
Peptide Molecular Mass
1,477.8140
Species
Human
Assay Type
Direct PRM
Matrix
SUM159 tryptic digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Q-Exactive
Internal Standard
25fmol
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
EASY-nLC 1000
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (1+) 6.4 6.7 4.8 7.5 7.7 4.4 9.9 10.2 6.5 15 15 15
y9 (1+) 5.4 7.3 4.2 7.7 8 4.1 9.4 10.8 5.9 15 15 15
y11 (1+) 6.4 6.4 4.3 8.8 8.3 3.1 10.9 10.5 5.3 15 15 15
sum 6 6.6 4.3 7.7 7.9 3.7 9.8 10.3 5.7 15 15 15


Additional Resources and Comments