LMTK2, CPTAC-6031 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
LMTK2	AATYK2, BREK, KIAA1079, KPI2, LMR2

Sequence Length (AA)	Molecular Weight (Da)
1503	164900

Protein Name
Serine/threonine-protein kinase LMTK2

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MPGPPALRRR	LLLLLLVLLI	AGSAGAAPLP	QTGAGEAPPA	AEVSSSFVIL
60	70	80	90	100
CVCSLIILIV	LIANCVSCCK	DPEIDFKEFE	DNFDDEIDFT	PPAEDTPSVQ
110	120	130	140	150
SPAEVFTLSV	PNISLPAPSQ	FQPSVEGLKS	QVARHSLNYI	QEIGNGWFGK
160	170	180	190	200
VLLGEIYTGT	SVARVIVKEL	KASANPKEQD	TFLKNGEPYY	ILQHPNILQC
210	220	230	240	250
VGQCVEAIPY	LLVFEFCDLG	DLKAYLRSEQ	EHMRGDSQTM	LLQRMACEVA
260	270	280	290	300
AGLAAMHKLH	FLHSDLALRN	CFLTSDLNVK	VGDYGIGFSR	YKEDYIETDD
310	320	330	340	350
KKVFPLRWTA	PELVTSFQDR	LLTADQTKYS	NIWSLGVTLW	ELFDNAAQPY
360	370	380	390	400
SNLSNLDVLN	QVIRERDTKL	PKPQLEQPYS	DRWYEVLQFC	WLSPEKRPAA
410	420	430	440	450
EDVHRLLTYL	RLQSQRDSEV	DFEQQWNALK	PNTNSRDSSN	NAAFPILDHF
460	470	480	490	500
ARDRLGREME	EVLTVTETSQ	GLSFEYVWEA	AKHDHFDERS	RGHLDEGLSY
510	520	530	540	550
TSIFYPVEVF	ESSLSDPGPG	KQDDSGQDVP	LRVPGVVPVF	DAHNLSVGSD
560	570	580	590	600
YYIQLEEKSG	SNLELDYPPA	LLTTDMDNPE	RTGPELSQLT	ALRSVELEES
610	620	630	640	650
STDEDFFQSS	TDPKDSSLPG	DLHVTSGPES	PFNNIFNDVD	KSEDLPSHQK
660	670	680	690	700
IFDLMELNGV	QADFKPATLS	SSLDNPKESV	ITGHFEKEKP	RKIFDSEPLC
710	720	730	740	750
LSDNLMHQDN	FDPLNVQELS	ENFLFLQEKN	LLKGSLSSKE	HINDLQTELK
760	770	780	790	800
NAGFTEAMLE	TSCRNSLDTE	LQFAENKPGL	SLLQENVSTK	GDDTDVMLTG
810	820	830	840	850
DTLSTSLQSS	PEVQVPPTSF	ETEETPRRVP	PDSLPTQGET	QPTCLDVIVP
860	870	880	890	900
EDCLHQDISP	DAVTVPVEIL	STDARTHSLD	NRSQDSPGES	EETLRLTESD
910	920	930	940	950
SVLADDILAS	RVSVGSSLPE	LGQELHNKPF	SEDHHSHRRL	EKNLEAVETL
960	970	980	990	1000
NQLNSKDAAK	EAGLVSALSS	DSTSQDSLLE	DSLSAPFPAS	EPSLETPDSL
1010	1020	1030	1040	1050
ESVDVHEALL	DSLGSHTPQK	LVPPDKPADS	GYETENLESP	EWTLHPAPEG
1060	1070	1080	1090	1100
TADSEPATTG	DGGHSGLPPN	PVIVISDAGD	GHRGTEVTPE	TFTAGSQGSY
1110	1120	1130	1140	1150
RDSAYFSDND	SEPEKRSEEV	PGTSPSALVL	VQEQPLPEPV	LPEQSPAAQD
1160	1170	1180	1190	1200
SCLEARKSQP	DESCLSALHN	SSDLELRATP	EPAQTGVPQQ	VHPTEDEASS
1210	1220	1230	1240	1250
PWSVLNAELS	SGDDFETQDD	RPCTLASTGT	NTNELLAYTN	SALDKSLSSH
1260	1270	1280	1290	1300
SEGPKLKEPD	IEGKYLGKLG	VSGMLDLSED	GMDADEEDEN	SDDSDEDLRA
1310	1320	1330	1340	1350
FNLHSLSSES	EDETEHPVPI	ILSNEDGRHL	RSLLKPTAAN	APDPLPEDWK
1360	1370	1380	1390	1400
KEKKAVTFFD	DVTVYLFDQE	TPTKELGPCG	GEACGPDLSG	PAPASGSPYL
1410	1420	1430	1440	1450
SRCINSESST	DEEGGGFEWD	DDFSPDPFMS	KTTSNLLSSK	PSLQTSKYFS
1460	1470	1480	1490	1500
PPPPARSTEQ	SWPHSAPYSR	FSISPANIAS	FSLTHLTDSD	IEQGGSSEDG
1503
EKD

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for non-CPTAC-6031 Collapse assay details

Data source: Panorama

Official Gene Symbol: LMTK2
Peptide Sequence: VGDYGIGFSR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 281
Peptide End: 290

CPTAC ID: non-CPTAC-6031
Peptide Molecular Mass: 1,069.5193
Species: Human
Assay Type: Direct PRM
Matrix: SUM159 tryptic digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Q-Exactive
Internal Standard: 25fmol
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: EASY-nLC 1000
Column Packing: C18
Column Dimensions: 75 µm x 50 cm
Flow Rate: 300nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y7 (1+)	2.9	2.7	2	4.9	2.4	2.7	5.7	3.6	3.4	15	15	15
y6 (1+)	3.7	1.6	2.1	5.2	1.9	3.5	6.4	2.5	4.1	15	15	15
y4 (1+)	2.1	2.2	2.2	3.4	2.5	4.4	4	3.3	4.9	15	15	15
sum	2	1.8	0.9	3.9	1.9	2.9	4.4	2.6	3	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

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Comments

Assay Details for non-CPTAC-6030 Collapse assay details

Data source: Panorama

Official Gene Symbol: LMTK2
Peptide Sequence: VLLGEIYTGTSVAR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 151
Peptide End: 164

CPTAC ID: non-CPTAC-6030
Peptide Molecular Mass: 1,477.8140
Species: Human
Assay Type: Direct PRM
Matrix: SUM159 tryptic digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend