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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

MAP3K10

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
MAP3K10 MLK2, MST
Sequence Length (AA) Molecular Weight (Da)
954 103694
Protein Name
Mitogen-activated protein kinase kinase kinase 10
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MEEEEGAVAK EWGTTPAGPV WTAVFDYEAA GDEELTLRRG DRVQVLSQDC
60 70 80 90 100
AVSGDEGWWT GQLPSGRVGV FPSNYVAPGA PAAPAGLQLP QEIPFHELQL
110 120 130 140 150
EEIIGVGGFG KVYRALWRGE EVAVKAARLD PEKDPAVTAE QVCQEARLFG
160 170 180 190 200
ALQHPNIIAL RGACLNPPHL CLVMEYARGG ALSRVLAGRR VPPHVLVNWA
210 220 230 240 250
VQVARGMNYL HNDAPVPIIH RDLKSINILI LEAIENHNLA DTVLKITDFG
260 270 280 290 300
LAREWHKTTK MSAAGTYAWM APEVIRLSLF SKSSDVWSFG VLLWELLTGE
310 320 330 340 350
VPYREIDALA VAYGVAMNKL TLPIPSTCPE PFARLLEECW DPDPHGRPDF
360 370 380 390 400
GSILKRLEVI EQSALFQMPL ESFHSLQEDW KLEIQHMFDD LRTKEKELRS
410 420 430 440 450
REEELLRAAQ EQRFQEEQLR RREQELAERE MDIVERELHL LMCQLSQEKP
460 470 480 490 500
RVRKRKGNFK RSRLLKLREG GSHISLPSGF EHKITVQASP TLDKRKGSDG
510 520 530 540 550
ASPPASPSII PRLRAIRLTP VDCGGSSSGS SSGGSGTWSR GGPPKKEELV
560 570 580 590 600
GGKKKGRTWG PSSTLQKERV GGEERLKGLG EGSKQWSSSA PNLGKSPKHT
610 620 630 640 650
PIAPGFASLN EMEEFAEAED GGSSVPPSPY STPSYLSVPL PAEPSPGARA
660 670 680 690 700
PWEPTPSAPP ARWGHGARRR CDLALLGCAT LLGAVGLGAD VAEARAADGE
710 720 730 740 750
EQRRWLDGLF FPRAGRFPRG LSPPARPHGR REDVGPGLGL APSATLVSLS
760 770 780 790 800
SVSDCNSTRS LLRSDSDEAA PAAPSPPPSP PAPTPTPSPS TNPLVDLELE
810 820 830 840 850
SFKKDPRQSL TPTHVTAACA VSRGHRRTPS DGALGQRGPP EPAGHGPGPR
860 870 880 890 900
DLLDFPRLPD PQALFPARRR PPEFPGRPTT LTFAPRPRPA ASRPRLDPWK
910 920 930 940 950
LVSFGRTLTI SPPSRPDTPE SPGPPSVQPT LLDMDMEGQN QDSTVPLCGA
954
HGSH

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt


Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-6032 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAP3K10
Peptide Sequence
ITVQASPTLDK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
484
Peptide End
494
CPTAC ID
non-CPTAC-6032
Peptide Molecular Mass
1,171.6449
Species
Human
Assay Type
Direct PRM
Matrix
SUM159 tryptic digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Q-Exactive
Internal Standard
25fmol
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
EASY-nLC 1000
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (1+) 1.7 2.4 1.8 1.9 5.1 4.2 2.5 5.6 4.6 15 15 15
y6 (1+) 1.8 2.1 1.5 2 4.4 4.2 2.7 4.9 4.5 15 15 15
y7 (1+) 2 2.1 1.1 2.9 4.7 5 3.5 5.1 5.1 15 15 15
sum 1 1.8 1.2 1.6 4.6 4.4 1.9 4.9 4.6 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments

Assay Details for non-CPTAC-6033 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAP3K10
Peptide Sequence
LPDPQALFPAR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
858
Peptide End
868
CPTAC ID
non-CPTAC-6033
Peptide Molecular Mass
1,223.6663
Species
Human
Assay Type
Direct PRM
Matrix
SUM159 tryptic digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Q-Exactive
Internal Standard
25fmol
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
EASY-nLC 1000
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300nL/min

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 3.5 3.1 2 4.1 3.8 3.1 5.4 4.9 3.7 15 15 15
y5 (1+) 3.6 3.6 2.4 4.1 3.7 4.3 5.5 5.2 4.9 15 15 15
y8 (1+) 2 3.7 1.8 2.8 4 2.9 3.4 5.4 3.4 15 15 15
sum 2.3 3.4 1.8 3 3.8 3.1 3.8 5.1 3.6 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments