Overview Data source: UniProt

Official Gene Symbol Other Aliases
MAPK3 ERK1,PRKM3
Sequence Length (AA) Molecular Weight (Da)
379 43136
Protein Name
Mitogen-activated protein kinase 3
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MAAAAAQGGG GGEPRRTEGV GPGVPGEVEM VKGQPFDVGP RYTQLQYIGE
60 70 80 90 100
GAYGMVSSAY DHVRKTRVAI KKISPFEHQT YCQRTLREIQ ILLRFRHENV
110 120 130 140 150
IGIRDILRAS TLEAMRDVYI VQDLMETDLY KLLKSQQLSN DHICYFLYQI
160 170 180 190 200
LRGLKYIHSA NVLHRDLKPS NLLINTTCDL KICDFGLARI ADPEHDHTGF
210 220 230 240 250
LTEYVATRWY RAPEIMLNSK GYTKSIDIWS VGCILAEMLS NRPIFPGKHY
260 270 280 290 300
LDQLNHILGI LGSPSQEDLN CIINMKARNY LQSLPSKTKV AWAKLFPKSD
310 320 330 340 350
SKALDLLDRM LTFNPNKRIT VEEALAHPYL EQYYDPTDEP VAEEPFTFAM
360 370 379
ELDDLPKERL KELIFQETAR FQPGVLEAP

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-1357 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAPK3
Peptide Modified Sequence
IADPEHDHTGFLT[+80.0]EY[+80.0]VATR
Modification Type
Phospho (ST), Phospho (Y)
Protein - Site of Modification
202, 204
Peptide - Site of Modification
13, 15
Peptide Start
190
Peptide End
208
CPTAC ID
CPTAC-1357
Peptide Molecular Mass
2,330.9610
Species
Homo sapiens (Human)
Assay Type
Enrichment PRM
Enrichment Method
N/A
Matrix
digested cell lysate
Submitting Laboratory
Broad Institute
Submitting Lab PI
Steven A. Carr

Publication

View Details (opens in a new window)

Mol Cell Proteomics. 2016 May;15(5):1622-41. doi: 10.1074/mcp.M116.058354. Epub 2016 Feb 24. Reduced-representation Phosphosignatures Measured by Quantitative Targeted MS Capture Cellular States and Enable Large-scale Comparison of Drug-induced Phenotypes. Abelin JG1, Patel J1, Lu X1, Feeney CM1, Fagbami L1, Creech AL1, Hu R1, Lam D1, Davison D1, Pino L1, Qiao JW1, Kuhn E1, Officer A1, Li J2, Abbatiello S1, Subramanian A1, Sidman R2, Snyder E3, Carr SA1, Jaffe JD4.

View Details (opens in a new window)

Mol Cell Proteomics. 2014 Jul;13(7):1690-704. doi: 10.1074/mcp.M113.036392. Epub 2014 Apr 9. Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mertins P1, Yang F2, Liu T2, Mani DR3, Petyuk VA2, Gillette MA3, Clauser KR3, Qiao JW3, Gritsenko MA2, Moore RJ2, Levine DA4, Townsend R5, Erdmann-Gilmore P5, Snider JE5, Davies SR5, Ruggles KV6, Fenyo D6, Kitchens RT5, Li S5, Olvera N4, Dao F4, Rodriguez H7, Chan DW8, Liebler D9, White F10, Rodland KD2, Mills GB11, Smith RD2, Paulovich AG12, Ellis M5, Carr SA1


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Quantiva
Internal Standard
light stable isotope peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Easy NanoLC1000
Column Packing
Reprosil C18, 1.9um, 200A
Column Dimensions
0.075 x 150 mm
Flow Rate
200 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y16-98 (2+) 10.6 6 7.2 10 12.5 10.3 14.6 13.9 12.6 15 15 15
y5 (1+) 6.6 7.8 6.5 7.8 11.3 10.4 10.2 13.7 12.3 15 15 15
y7-98 (1+) 5.6 5.7 5 6.9 11.5 10.5 8.9 12.8 11.6 15 15 15
sum 5.6 5.5 4.3 6.9 11.1 9.8 8.9 12.4 10.7 15 15 15

Additional Resources and Comments


Assay Details for CPTAC-1358 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAPK3
Peptide Modified Sequence
IADPEHDHTGFLT[+80.0]EYVATR
Modification Type
Phospho (ST)
Protein - Site of Modification
202
Peptide - Site of Modification
13
Peptide Start
190
Peptide End
208
CPTAC ID
CPTAC-1358
Peptide Molecular Mass
2,250.9947
Species
Homo sapiens (Human)
Assay Type
Enrichment PRM
Enrichment Method
N/A
Matrix
digested cell lysate
Submitting Laboratory
Broad Institute
Submitting Lab PI
Steven A. Carr

Publication

View Details (opens in a new window)

Mol Cell Proteomics. 2016 May;15(5):1622-41. doi: 10.1074/mcp.M116.058354. Epub 2016 Feb 24. Reduced-representation Phosphosignatures Measured by Quantitative Targeted MS Capture Cellular States and Enable Large-scale Comparison of Drug-induced Phenotypes. Abelin JG1, Patel J1, Lu X1, Feeney CM1, Fagbami L1, Creech AL1, Hu R1, Lam D1, Davison D1, Pino L1, Qiao JW1, Kuhn E1, Officer A1, Li J2, Abbatiello S1, Subramanian A1, Sidman R2, Snyder E3, Carr SA1, Jaffe JD4.

View Details (opens in a new window)

Mol Cell Proteomics. 2014 Jul;13(7):1690-704. doi: 10.1074/mcp.M113.036392. Epub 2014 Apr 9. Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mertins P1, Yang F2, Liu T2, Mani DR3, Petyuk VA2, Gillette MA3, Clauser KR3, Qiao JW3, Gritsenko MA2, Moore RJ2, Levine DA4, Townsend R5, Erdmann-Gilmore P5, Snider JE5, Davies SR5, Ruggles KV6, Fenyo D6, Kitchens RT5, Li S5, Olvera N4, Dao F4, Rodriguez H7, Chan DW8, Liebler D9, White F10, Rodland KD2, Mills GB11, Smith RD2, Paulovich AG12, Ellis M5, Carr SA1


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Quantiva
Internal Standard
light stable isotope peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Easy NanoLC1000
Column Packing
Reprosil C18, 1.9um, 200A
Column Dimensions
0.075 x 150 mm
Flow Rate
200 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y16-98 (2+) 17.5 15.7 10.4 26.3 18.8 18.7 31.6 24.5 21.4 15 14 15
y6 (1+) 8.9 10.9 12.9 15.2 12.1 13.5 17.6 16.3 18.7 14 14 15
y7-98 (1+) 11.6 9.4 3.8 16.1 12.8 10.5 19.8 15.9 11.2 15 15 15
sum 6.7 10.1 5.1 12.1 13 7.9 13.8 16.5 9.4 15 15 15

Additional Resources and Comments


Assay Details for CPTAC-1359 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAPK3
Peptide Modified Sequence
IADPEHDHTGFLTEY[+80.0]VATR
Modification Type
Phospho (Y)
Protein - Site of Modification
204
Peptide - Site of Modification
15
Peptide Start
190
Peptide End
208
CPTAC ID
CPTAC-1359
Peptide Molecular Mass
2,250.9947
Species
Homo sapiens (Human)
Assay Type
Enrichment PRM
Enrichment Method
N/A
Matrix
digested cell lysate
Submitting Laboratory
Broad Institute
Submitting Lab PI
Steven A. Carr

Publication

View Details (opens in a new window)

Mol Cell Proteomics. 2016 May;15(5):1622-41. doi: 10.1074/mcp.M116.058354. Epub 2016 Feb 24. Reduced-representation Phosphosignatures Measured by Quantitative Targeted MS Capture Cellular States and Enable Large-scale Comparison of Drug-induced Phenotypes. Abelin JG1, Patel J1, Lu X1, Feeney CM1, Fagbami L1, Creech AL1, Hu R1, Lam D1, Davison D1, Pino L1, Qiao JW1, Kuhn E1, Officer A1, Li J2, Abbatiello S1, Subramanian A1, Sidman R2, Snyder E3, Carr SA1, Jaffe JD4.

View Details (opens in a new window)

Mol Cell Proteomics. 2014 Jul;13(7):1690-704. doi: 10.1074/mcp.M113.036392. Epub 2014 Apr 9. Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mertins P1, Yang F2, Liu T2, Mani DR3, Petyuk VA2, Gillette MA3, Clauser KR3, Qiao JW3, Gritsenko MA2, Moore RJ2, Levine DA4, Townsend R5, Erdmann-Gilmore P5, Snider JE5, Davies SR5, Ruggles KV6, Fenyo D6, Kitchens RT5, Li S5, Olvera N4, Dao F4, Rodriguez H7, Chan DW8, Liebler D9, White F10, Rodland KD2, Mills GB11, Smith RD2, Paulovich AG12, Ellis M5, Carr SA1


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Quantiva
Internal Standard
light stable isotope peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Easy NanoLC1000
Column Packing
Reprosil C18, 1.9um, 200A
Column Dimensions
0.075 x 150 mm
Flow Rate
200 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y16 (2+) 4.8 5.5 7.4 6.3 5.9 11.1 7.9 8.1 13.3 15 15 15
y5 (1+) 5.3 9.2 7 5.8 8.1 9 7.9 12.3 11.4 15 15 15
y7 (1+) 5.2 7.3 5.4 3.7 7.4 7.4 6.4 10.4 9.2 15 15 15
sum 4.3 6.3 5.8 4.3 6.2 8.3 6.1 8.8 10.1 15 15 15

Additional Resources and Comments


Assay Details for CPTAC-880 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAPK3
Peptide Modified Sequence
IADPEHDHTGFLTEY[+80.0]VATR
Modification Type
Phospho (Y)
Protein - Site of Modification
204
Peptide - Site of Modification
15
Peptide Start
190
Peptide End
208
CPTAC ID
CPTAC-880
Peptide Molecular Mass
2,250.9947
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 20.5 13.9 18.9 26 19.6 28.7 33.1 24 34.4 15 15 15
y4 (1+) 24.9 20.3 13.5 31.5 22.4 13.9 40.2 30.2 19.4 15 15 15
y5 (1+) 21.7 13.9 14.4 21.1 19.4 15.7 30.3 23.9 21.3 15 15 15
y6 (1+) 35.4 13.1 10 32.2 15 11.1 47.9 19.9 14.9 15 15 15
y7 (1+) 12.3 7 7 17.7 7.1 6 21.6 10 9.2 15 15 15
y8 (1+) 33.4 11.3 21.1 23.3 12.3 18.9 40.7 16.7 28.3 15 15 15
y9 (1+) 63.5 21.3 19.8 52.7 26 18.2 82.5 33.6 26.9 15 15 15
y10 (1+) 26.8 18.5 16 26.7 19.2 21.3 37.8 26.7 26.6 15 15 15
y11 (1+) 51.5 18.8 25.3 55.8 24.8 24.2 75.9 31.1 35 15 15 15
y12 (1+) 63.5 19.8 25.4 55.8 26.9 29.4 84.5 33.4 38.9 15 15 15
b13 (1+) 141.4 121.9 93.7 74 100.5 126.3 159.6 158 157.3 8 8 10
b12 (1+) 64.9 43.3 59.8 61.6 53.4 55.2 89.5 68.7 81.4 15 15 15
b11 (1+) 37 26.1 23.6 58 28.3 23.4 68.8 38.5 33.2 15 15 15
b10 (1+) 71.4 48 26.2 41.5 49.6 31 82.6 69 40.6 15 15 15
b9 (1+) 47.2 41.5 45.6 53.1 39.8 54.8 71 57.5 71.3 14 15 15
b8 (1+) 43.1 46.6 45.3 54.4 49.2 42.2 69.4 67.8 61.9 15 15 15
b7 (1+) 45.3 25.6 18.9 43.5 27.8 31.4 62.8 37.8 36.6 15 15 15
b6 (1+) 70.4 67.2 39.3 65.7 80.2 72.1 96.3 104.6 82.1 15 15 15
b5 (1+) 46.1 72.4 49.7 60.2 76.8 40.4 75.8 105.5 64 15 15 15
b4 (1+) 53.6 47.7 48.4 65 51 79.1 84.2 69.8 92.7 15 15 15
b3 (1+) 23.9 25.1 23 31.8 34.7 44.1 39.8 42.8 49.7 15 15 15
sum 11.5 5.5 4.7 15.1 5.7 5.6 19 7.9 7.3 15 15 15

Additional Resources and Comments


Assay Details for CPTAC-1050 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAPK3
Peptide Modified Sequence
IADPEHDHTGFLT[+80.0]EYVATR
Modification Type
Phospho (ST)
Protein - Site of Modification
202
Peptide - Site of Modification
13
Peptide Start
190
Peptide End
208
CPTAC ID
CPTAC-1050
Peptide Molecular Mass
2,250.9947
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (1+) 40.4 11.3 9.1 35.3 9.5 7 53.6 14.8 11.5 15 15 15
y4 (1+) 34.9 8.3 10.5 33.3 10.1 10.9 48.2 13.1 15.1 15 15 15
y3 (1+) 28.3 14.8 8 24.3 19 12.1 37.3 24.1 14.5 15 15 15
sum 16.5 4.9 5.2 14.4 6 7.2 21.9 7.7 8.9 15 15 15

Additional Resources and Comments


Assay Details for CPTAC-1051 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAPK3
Peptide Sequence
IADPEHDHTGFLTEYVATR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
190
Peptide End
208
CPTAC ID
CPTAC-1051
Peptide Molecular Mass
2,171.0283
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (1+) 16.4 10.4 11.5 18.3 10.9 11.9 24.6 15.1 16.5 15 15 15
y4 (1+) 17.4 7.1 10.7 18.1 6.7 9.9 25.1 9.8 14.6 15 15 15
y3 (1+) 12.9 7.4 7.1 12 7.9 7.1 17.6 10.8 10 15 15 15
sum 8.7 4.3 7 7.5 3.7 5.3 11.5 5.7 8.8 15 15 15

Additional Resources and Comments