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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
CDC42 N/A
Sequence Length (AA) Molecular Weight (Da)
191 21259
Protein Name
Cell division control protein 42 homolog
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MQTIKCVVVG DGAVGKTCLL ISYTTNKFPS EYVPTVFDNY AVTVMIGGEP
60 70 80 90 100
YTLGLFDTAG QEDYDRLRPL SYPQTDVFLV CFSVVSPSSF ENVKEKWVPE
110 120 130 140 150
ITHHCPKTPF LLVGTQIDLR DDPSTIEKLA KNKQKPITPE TAEKLARDLK
160 170 180 190 191
AVKYVECSAL TQKGLKNVFD EAILAALEPP EPKKSRRCVL L

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node
to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1601 Collapse assay details

Data source: Panorama

Official Gene Symbol
CDC42
Peptide Sequence
NVFDEAILAALEPPEPK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
167
Peptide End
183
CPTAC ID
CPTAC-1601
Peptide Molecular Mass
1,851.9618
Species
Homo sapiens (Human)
Assay Type
Direct MRM or SRM
Matrix
Ovarian cancer tumor tissue lysate
Submitting Laboratory
Pacific Northwest National Laboratory
Submitting Lab PI
Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Waters nanoACQUITY (Part Number 176016000)
Column Packing
Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions
100 um x 100 mm
Flow Rate
0.5 uL/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-DirectSRM3

CPTAC-1593:
ABI2.
ALFDSYTNLER
CPTAC-1594:
ALDH2.
VAEQTPLTALYVANLIK
CPTAC-1595:
ANP32B.
ELVLDNC[+57.0]K
CPTAC-1596:
AP1B1.
LLSTDPVAAK
CPTAC-1597:
ARPC2.
VFMQEFK
CPTAC-1598:
BRD2.
LMFSNC[+57.0]YK
CPTAC-1599:
CDH1.
GLDARPEVTR
CPTAC-1600:
CBX3.
DSDEADLVLAK
CPTAC-1601:
CDC42.
NVFDEAILAALEPPEPK
CPTAC-1602:
CDK9.
IGQGTFGEVFK
CPTAC-1603:
C20orf27.
LLSVVPVPEGYSVK
CPTAC-1604:
DOCK2.
DILLPVITK
CPTAC-1605:
EHD2.
LEGHGLPANLPR
CPTAC-1606:
EML1.
VQMQEDDIQLLK
CPTAC-1607:
ENY2.
ALVPDSVK
CPTAC-1608:
FIGNL1.
GILLFGPPGTGK
CPTAC-1609:
GRB2.
ATADDELSFK
CPTAC-1610:
GTPBP1.
VGDNDFLEVR
CPTAC-1611:
C15orf38-AP3S2.
TGASWTDNIMAQK
CPTAC-1612:
HM13.
SFEAPIK
CPTAC-1613:
EPHX1.
DVELLYPVK
CPTAC-1614:
ITGA1.
FGTAIAAVK
CPTAC-1615:
PRKCA.
GTEELYAIK
CPTAC-1616:
LNPK.
IVEYLVGDGPQNR
CPTAC-1617:
LRP1.
AALSGANVLTLIEK
CPTAC-1618:
MCM2.
GLALALFGGEPK
CPTAC-1619:
MTA1.
YQADITDLLK
CPTAC-1620:
MYO1G.
TLVTLEQSR
CPTAC-1621:
NEK7.
VQIFDLMDAK
CPTAC-1622:
NF1.
LVFIDC[+57.0]PGK
CPTAC-1623:
FAM129A.
VLTSEDEYNLLSDR
CPTAC-1624:
NMRAL1.
LGFPGAR
CPTAC-1625:
OSBPL10.
LLEHGEEYVFTLPSAYAR
CPTAC-1626:
PIK3R1.
TATGYGFAEPYNLYSSLK
CPTAC-1627:
PACSIN2.
AIYHDLEQSIR
CPTAC-1628:
PAK2.
SVIDPVPAPVGDSHVDGAAK
CPTAC-1629:
PELP1.
TGSAVAPVHPPNR
CPTAC-1630:
PDGFRB.
LLGEVGTLQFAELHR
CPTAC-1631:
PIK3CA.
LINLTDILK
CPTAC-1632:
ARL6IP5.
AWDDFFPGSDR
CPTAC-1633:
RNF121.
GWC[+57.0]IVGK
CPTAC-1634:
POLR1E.
HFVGILNK
CPTAC-1635:
PDCD11.
VTPNEGLTVSFPFGK
CPTAC-1636:
SMARCA5.
TEQEEDEELLTESSK
CPTAC-1637:
SSBP1.
SGDSEVYQLGDVSQK
CPTAC-1638:
STAT5B.
ATQLLEGLVQELQK
CPTAC-1639:
STAT6.
SVSWSQFNK
CPTAC-1640:
STRN.
SELTDSASVLDNFK
CPTAC-1641:
SVIL.
YGSFEEAEASYPILNR
CPTAC-1642:
VARS.
NVIHPFLSR
CPTAC-1643:
CCT6A.
GIDPFSLDALSK
CPTAC-1644:
TGM2.
SEGTYC[+57.0]C[+57.0]GPVPVR
CPTAC-1645:
TIA1.
AAFAPFGR
CPTAC-1646:
TMEM43.
LEDPHVDIIR
CPTAC-1647:
TRA2A.
YGPLSGVNVVYDQR

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (1+) 6.8 4.8 3.5 13 28.2 28.5 14.7 28.6 28.7 15 15 15
y9 (1+) 11.9 5.3 13.4 21.9 28.8 29.5 24.9 29.3 32.4 15 15 15
y10 (1+) 11 6.4 10.1 13.4 27.7 32.3 17.3 28.4 33.8 15 15 15
sum 4.8 4.3 3.3 9.1 27.9 28.2 10.3 28.2 28.4 15 15 15


Additional Resources and Comments