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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

TGM2

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
TGM2 N/A
Sequence Length (AA) Molecular Weight (Da)
687 77329
Protein Name
Protein-glutamine gamma-glutamyltransferase 2
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MAEELVLERC DLELETNGRD HHTADLCREK LVVRRGQPFW LTLHFEGRNY
60 70 80 90 100
EASVDSLTFS VVTGPAPSQE AGTKARFPLR DAVEEGDWTA TVVDQQDCTL
110 120 130 140 150
SLQLTTPANA PIGLYRLSLE ASTGYQGSSF VLGHFILLFN AWCPADAVYL
160 170 180 190 200
DSEEERQEYV LTQQGFIYQG SAKFIKNIPW NFGQFEDGIL DICLILLDVN
210 220 230 240 250
PKFLKNAGRD CSRRSSPVYV GRVVSGMVNC NDDQGVLLGR WDNNYGDGVS
260 270 280 290 300
PMSWIGSVDI LRRWKNHGCQ RVKYGQCWVF AAVACTVLRC LGIPTRVVTN
310 320 330 340 350
YNSAHDQNSN LLIEYFRNEF GEIQGDKSEM IWNFHCWVES WMTRPDLQPG
360 370 380 390 400
YEGWQALDPT PQEKSEGTYC CGPVPVRAIK EGDLSTKYDA PFVFAEVNAD
410 420 430 440 450
VVDWIQQDDG SVHKSINRSL IVGLKISTKS VGRDEREDIT HTYKYPEGSS
460 470 480 490 500
EEREAFTRAN HLNKLAEKEE TGMAMRIRVG QSMNMGSDFD VFAHITNNTA
510 520 530 540 550
EEYVCRLLLC ARTVSYNGIL GPECGTKYLL NLNLEPFSEK SVPLCILYEK
560 570 580 590 600
YRDCLTESNL IKVRALLVEP VINSYLLAER DLYLENPEIK IRILGEPKQK
610 620 630 640 650
RKLVAEVSLQ NPLPVALEGC TFTVEGAGLT EEQKTVEIPD PVEAGEEVKV
660 670 680 687
RMDLLPLHMG LHKLVVNFES DKLKAVKGFR NVIIGPA

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt


Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-281 Collapse assay details

Data source: Panorama

Official Gene Symbol
TGM2
Peptide Modified Sequence
SEGTYC[+57.0]C[+57.0]GPVPVR
Modification Type
Carbamidomethyl Cysteine, Carbamidomethyl Cysteine
Protein - Site of Modification
370, 371
Peptide - Site of Modification
6, 7
Peptide Start
365
Peptide End
377
CPTAC ID
CPTAC-281
Peptide Molecular Mass
1,480.6439
Species
Homo sapiens (Human)
Assay Type
Direct MRM or SRM
Matrix
cell line lysate pool
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-DirectSRM3

CPTAC-1593:
ABI2.
ALFDSYTNLER 		CPTAC-1594:
ALDH2.
VAEQTPLTALYVANLIK 		CPTAC-1595:
ANP32B.
ELVLDNC[+57.0]K
CPTAC-1596:
AP1B1.
LLSTDPVAAK 		CPTAC-1597:
ARPC2.
VFMQEFK 		CPTAC-1598:
BRD2.
LMFSNC[+57.0]YK
CPTAC-1599:
CDH1.
GLDARPEVTR 		CPTAC-1600:
CBX3.
DSDEADLVLAK 		CPTAC-1601:
CDC42.
NVFDEAILAALEPPEPK
CPTAC-1602:
CDK9.
IGQGTFGEVFK 		CPTAC-1603:
C20orf27.
LLSVVPVPEGYSVK 		CPTAC-1604:
DOCK2.
DILLPVITK
CPTAC-1605:
EHD2.
LEGHGLPANLPR 		CPTAC-1606:
EML1.
VQMQEDDIQLLK 		CPTAC-1607:
ENY2.
ALVPDSVK
CPTAC-1608:
FIGNL1.
GILLFGPPGTGK 		CPTAC-1609:
GRB2.
ATADDELSFK 		CPTAC-1610:
GTPBP1.
VGDNDFLEVR
CPTAC-1611:
C15orf38-AP3S2.
TGASWTDNIMAQK 		CPTAC-1612:
HM13.
SFEAPIK 		CPTAC-1613:
EPHX1.
DVELLYPVK
CPTAC-1614:
ITGA1.
FGTAIAAVK 		CPTAC-1615:
PRKCA.
GTEELYAIK 		CPTAC-1616:
LNPK.
IVEYLVGDGPQNR
CPTAC-1617:
LRP1.
AALSGANVLTLIEK 		CPTAC-1618:
MCM2.
GLALALFGGEPK 		CPTAC-1619:
MTA1.
YQADITDLLK
CPTAC-1620:
MYO1G.
TLVTLEQSR 		CPTAC-1621:
NEK7.
VQIFDLMDAK 		CPTAC-1622:
NF1.
LVFIDC[+57.0]PGK
CPTAC-1623:
FAM129A.
VLTSEDEYNLLSDR 		CPTAC-1624:
NMRAL1.
LGFPGAR 		CPTAC-1625:
OSBPL10.
LLEHGEEYVFTLPSAYAR
CPTAC-1626:
PIK3R1.
TATGYGFAEPYNLYSSLK 		CPTAC-1627:
PACSIN2.
AIYHDLEQSIR 		CPTAC-1628:
PAK2.
SVIDPVPAPVGDSHVDGAAK
CPTAC-1629:
PELP1.
TGSAVAPVHPPNR 		CPTAC-1630:
PDGFRB.
LLGEVGTLQFAELHR 		CPTAC-1631:
PIK3CA.
LINLTDILK
CPTAC-1632:
ARL6IP5.
AWDDFFPGSDR 		CPTAC-1633:
RNF121.
GWC[+57.0]IVGK 		CPTAC-1634:
POLR1E.
HFVGILNK
CPTAC-1635:
PDCD11.
VTPNEGLTVSFPFGK 		CPTAC-1636:
SMARCA5.
TEQEEDEELLTESSK 		CPTAC-1637:
SSBP1.
SGDSEVYQLGDVSQK
CPTAC-1638:
STAT5B.
ATQLLEGLVQELQK 		CPTAC-1639:
STAT6.
SVSWSQFNK 		CPTAC-1640:
STRN.
SELTDSASVLDNFK
CPTAC-1641:
SVIL.
YGSFEEAEASYPILNR 		CPTAC-1642:
VARS.
NVIHPFLSR 		CPTAC-1643:
CCT6A.
GIDPFSLDALSK
CPTAC-1644:
TGM2.
SEGTYC[+57.0]C[+57.0]GPVPVR 		CPTAC-1645:
TIA1.
AAFAPFGR 		CPTAC-1646:
TMEM43.
LEDPHVDIIR
CPTAC-1647:
TRA2A.
YGPLSGVNVVYDQR

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y9 (1+) 11.2 9.5 15.3 12.9 10.5 12.3 17.1 14.2 19.6 15 25 15
y8 (1+) 9.8 10.5 14.6 13.5 10.6 15 16.7 14.9 20.9 15 25 15
y7 (1+) 10.6 10 14.5 11.3 10.6 12.7 15.5 14.6 19.3 15 25 15
sum 9.3 8.7 13.8 12 9.5 12.8 15.2 12.9 18.8 15 25 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments

Assay Details for CPTAC-280 Collapse assay details

Data source: Panorama

Official Gene Symbol
TGM2
Peptide Sequence
VVTNYNSAHDQNSNLLIEYFR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
297
Peptide End
317
CPTAC ID
CPTAC-280
Peptide Molecular Mass
2,496.2034
Species
Homo sapiens (Human)
Assay Type
Direct MRM or SRM
Matrix
cell line lysate pool
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-DirectSRM3

CPTAC-1593:
ABI2.
ALFDSYTNLER 		CPTAC-1594:
ALDH2.
VAEQTPLTALYVANLIK 		CPTAC-1595:
ANP32B.
ELVLDNC[+57.0]K
CPTAC-1596:
AP1B1.
LLSTDPVAAK 		CPTAC-1597:
ARPC2.
VFMQEFK 		CPTAC-1598:
BRD2.
LMFSNC[+57.0]YK
CPTAC-1599:
CDH1.
GLDARPEVTR 		CPTAC-1600:
CBX3.
DSDEADLVLAK 		CPTAC-1601:
CDC42.
NVFDEAILAALEPPEPK
CPTAC-1602:
CDK9.
IGQGTFGEVFK 		CPTAC-1603:
C20orf27.
LLSVVPVPEGYSVK 		CPTAC-1604:
DOCK2.
DILLPVITK
CPTAC-1605:
EHD2.
LEGHGLPANLPR 		CPTAC-1606:
EML1.
VQMQEDDIQLLK 		CPTAC-1607:
ENY2.
ALVPDSVK
CPTAC-1608:
FIGNL1.
GILLFGPPGTGK 		CPTAC-1609:
GRB2.
ATADDELSFK 		CPTAC-1610:
GTPBP1.
VGDNDFLEVR
CPTAC-1611:
C15orf38-AP3S2.
TGASWTDNIMAQK 		CPTAC-1612:
HM13.
SFEAPIK 		CPTAC-1613:
EPHX1.
DVELLYPVK
CPTAC-1614:
ITGA1.
FGTAIAAVK 		CPTAC-1615:
PRKCA.
GTEELYAIK 		CPTAC-1616:
LNPK.
IVEYLVGDGPQNR
CPTAC-1617:
LRP1.
AALSGANVLTLIEK 		CPTAC-1618:
MCM2.
GLALALFGGEPK 		CPTAC-1619:
MTA1.
YQADITDLLK
CPTAC-1620:
MYO1G.
TLVTLEQSR 		CPTAC-1621:
NEK7.
VQIFDLMDAK 		CPTAC-1622:
NF1.
LVFIDC[+57.0]PGK
CPTAC-1623:
FAM129A.
VLTSEDEYNLLSDR 		CPTAC-1624:
NMRAL1.
LGFPGAR 		CPTAC-1625:
OSBPL10.
LLEHGEEYVFTLPSAYAR
CPTAC-1626:
PIK3R1.
TATGYGFAEPYNLYSSLK 		CPTAC-1627:
PACSIN2.
AIYHDLEQSIR 		CPTAC-1628:
PAK2.
SVIDPVPAPVGDSHVDGAAK
CPTAC-1629:
PELP1.
TGSAVAPVHPPNR 		CPTAC-1630:
PDGFRB.
LLGEVGTLQFAELHR 		CPTAC-1631:
PIK3CA.
LINLTDILK
CPTAC-1632:
ARL6IP5.
AWDDFFPGSDR 		CPTAC-1633:
RNF121.
GWC[+57.0]IVGK 		CPTAC-1634:
POLR1E.
HFVGILNK
CPTAC-1635:
PDCD11.
VTPNEGLTVSFPFGK 		CPTAC-1636:
SMARCA5.
TEQEEDEELLTESSK 		CPTAC-1637:
SSBP1.
SGDSEVYQLGDVSQK
CPTAC-1638:
STAT5B.
ATQLLEGLVQELQK 		CPTAC-1639:
STAT6.
SVSWSQFNK 		CPTAC-1640:
STRN.
SELTDSASVLDNFK
CPTAC-1641:
SVIL.
YGSFEEAEASYPILNR 		CPTAC-1642:
VARS.
NVIHPFLSR 		CPTAC-1643:
CCT6A.
GIDPFSLDALSK
CPTAC-1644:
TGM2.
SEGTYC[+57.0]C[+57.0]GPVPVR 		CPTAC-1645:
TIA1.
AAFAPFGR 		CPTAC-1646:
TMEM43.
LEDPHVDIIR
CPTAC-1647:
TRA2A.
YGPLSGVNVVYDQR

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 30.6 28.2 13.3 41.8 34.2 11.8 51.8 44.3 17.8 15 15 15
y18 (2+) 37 21.9 24 42.7 26.6 32.5 56.5 34.5 40.4 15 15 15
y17 (2+) 24.5 19.2 31.3 40.8 28.8 39.5 47.6 34.6 50.4 15 15 15
sum 15 9.4 10.1 28.2 18.4 12.6 31.9 20.7 16.1 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments

Assay Details for CPTAC-1644 Collapse assay details

Data source: Panorama

Official Gene Symbol
TGM2
Peptide Modified Sequence
SEGTYC[+57.0]C[+57.0]GPVPVR
Modification Type
Carbamidomethyl (C), Carbamidomethyl (C)
Protein - Site of Modification
370, 371
Peptide - Site of Modification
6, 7
Peptide Start
365
Peptide End
377
CPTAC ID
CPTAC-1644
Peptide Molecular Mass
1,480.6439
Species
Homo sapiens (Human)
Assay Type
Direct MRM or SRM
Matrix
Ovarian cancer tumor tissue lysate
Submitting Laboratory
Pacific Northwest National Laboratory
Submitting Lab PI
Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Waters nanoACQUITY (Part Number 176016000)
Column Packing
Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions
100 um x 100 mm
Flow Rate
0.5 uL/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-DirectSRM3

CPTAC-1593:
ABI2.
ALFDSYTNLER 		CPTAC-1594:
ALDH2.
VAEQTPLTALYVANLIK 		CPTAC-1595:
ANP32B.
ELVLDNC[+57.0]K
CPTAC-1596:
AP1B1.
LLSTDPVAAK 		CPTAC-1597:
ARPC2.
VFMQEFK 		CPTAC-1598:
BRD2.
LMFSNC[+57.0]YK
CPTAC-1599:
CDH1.
GLDARPEVTR 		CPTAC-1600:
CBX3.
DSDEADLVLAK 		CPTAC-1601:
CDC42.
NVFDEAILAALEPPEPK
CPTAC-1602:
CDK9.
IGQGTFGEVFK 		CPTAC-1603:
C20orf27.
LLSVVPVPEGYSVK 		CPTAC-1604:
DOCK2.
DILLPVITK
CPTAC-1605:
EHD2.
LEGHGLPANLPR 		CPTAC-1606:
EML1.
VQMQEDDIQLLK 		CPTAC-1607:
ENY2.
ALVPDSVK
CPTAC-1608:
FIGNL1.
GILLFGPPGTGK 		CPTAC-1609:
GRB2.
ATADDELSFK 		CPTAC-1610:
GTPBP1.
VGDNDFLEVR
CPTAC-1611:
C15orf38-AP3S2.
TGASWTDNIMAQK 		CPTAC-1612:
HM13.
SFEAPIK 		CPTAC-1613:
EPHX1.
DVELLYPVK
CPTAC-1614:
ITGA1.
FGTAIAAVK 		CPTAC-1615:
PRKCA.
GTEELYAIK 		CPTAC-1616:
LNPK.
IVEYLVGDGPQNR
CPTAC-1617:
LRP1.
AALSGANVLTLIEK 		CPTAC-1618:
MCM2.
GLALALFGGEPK 		CPTAC-1619:
MTA1.
YQADITDLLK
CPTAC-1620:
MYO1G.
TLVTLEQSR 		CPTAC-1621:
NEK7.
VQIFDLMDAK 		CPTAC-1622:
NF1.
LVFIDC[+57.0]PGK
CPTAC-1623:
FAM129A.
VLTSEDEYNLLSDR 		CPTAC-1624:
NMRAL1.
LGFPGAR 		CPTAC-1625:
OSBPL10.
LLEHGEEYVFTLPSAYAR
CPTAC-1626:
PIK3R1.
TATGYGFAEPYNLYSSLK 		CPTAC-1627:
PACSIN2.
AIYHDLEQSIR 		CPTAC-1628:
PAK2.
SVIDPVPAPVGDSHVDGAAK
CPTAC-1629:
PELP1.
TGSAVAPVHPPNR 		CPTAC-1630:
PDGFRB.
LLGEVGTLQFAELHR 		CPTAC-1631:
PIK3CA.
LINLTDILK
CPTAC-1632:
ARL6IP5.
AWDDFFPGSDR 		CPTAC-1633:
RNF121.
GWC[+57.0]IVGK 		CPTAC-1634:
POLR1E.
HFVGILNK
CPTAC-1635:
PDCD11.
VTPNEGLTVSFPFGK 		CPTAC-1636:
SMARCA5.
TEQEEDEELLTESSK 		CPTAC-1637:
SSBP1.
SGDSEVYQLGDVSQK
CPTAC-1638:
STAT5B.
ATQLLEGLVQELQK 		CPTAC-1639:
STAT6.
SVSWSQFNK 		CPTAC-1640:
STRN.
SELTDSASVLDNFK
CPTAC-1641:
SVIL.
YGSFEEAEASYPILNR 		CPTAC-1642:
VARS.
NVIHPFLSR 		CPTAC-1643:
CCT6A.
GIDPFSLDALSK
CPTAC-1644:
TGM2.
SEGTYC[+57.0]C[+57.0]GPVPVR 		CPTAC-1645:
TIA1.
AAFAPFGR 		CPTAC-1646:
TMEM43.
LEDPHVDIIR
CPTAC-1647:
TRA2A.
YGPLSGVNVVYDQR

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 6.5 2.2 2.7 11 2.9 7 12.8 3.6 7.5 15 15 15
y6 (1+) 13.6 5.9 4.5 14 4.4 5.8 19.5 7.4 7.3 15 15 15
y7 (1+) 17.2 3.2 3.8 22 3.8 7 27.9 5 8 15 15 15
sum 7.5 1.8 2.6 11.5 2.5 6.4 13.7 3.1 6.9 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments