Show Nav →

Overview Data source: UniProt

Official Gene Symbol Other Aliases
EHD2 PAST2
Sequence Length (AA) Molecular Weight (Da)
543 61161
Protein Name
EH domain-containing protein 2
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MFSWLKRGGA RGQQPEAIRT VTSALKELYR TKLLPLEEHY RFGAFHSPAL
60 70 80 90 100
EDADFDGKPM VLVAGQYSTG KTSFIQYLLE QEVPGSRVGP EPTTDCFVAV
110 120 130 140 150
MHGDTEGTVP GNALVVDPDK PFRKLNPFGN TFLNRFMCAQ LPNQVLESIS
160 170 180 190 200
IIDTPGILSG AKQRVSRGYD FPAVLRWFAE RVDLIILLFD AHKLEISDEF
210 220 230 240 250
SEAIGALRGH EDKIRVVLNK ADMVETQQLM RVYGALMWAL GKVVGTPEVL
260 270 280 290 300
RVYIGSFWSQ PLLVPDNRRL FELEEQDLFR DIQGLPRHAA LRKLNDLVKR
310 320 330 340 350
ARLVRVHAYI ISYLKKEMPS VFGKENKKKQ LILKLPVIFA KIQLEHHISP
360 370 380 390 400
GDFPDCQKMQ ELLMAHDFTK FHSLKPKLLE ALDEMLTHDI AKLMPLLRQE
410 420 430 440 450
ELESTEVGVQ GGAFEGTHMG PFVERGPDEA MEDGEEGSDD EAEWVVTKDK
460 470 480 490 500
SKYDEIFYNL APADGKLSGS KAKTWMVGTK LPNSVLGRIW KLSDVDRDGM
510 520 530 540 543
LDDEEFALAS HLIEAKLEGH GLPANLPRRL VPPSKRRHKG SAE

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node
to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

loading

Loader

Assay Details for CPTAC-1605 Collapse assay details

Data source: Panorama

Official Gene Symbol
EHD2
Peptide Sequence
LEGHGLPANLPR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
517
Peptide End
528
CPTAC ID
CPTAC-1605
Peptide Molecular Mass
1,272.6939
Species
Homo sapiens (Human)
Assay Type
Direct MRM or SRM
Matrix
Ovarian cancer tumor tissue lysate
Submitting Laboratory
Pacific Northwest National Laboratory
Submitting Lab PI
Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Waters nanoACQUITY (Part Number 176016000)
Column Packing
Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions
100 um x 100 mm
Flow Rate
0.5 uL/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-DirectSRM3

CPTAC-1593:
ABI2.
ALFDSYTNLER
CPTAC-1594:
ALDH2.
VAEQTPLTALYVANLIK
CPTAC-1595:
ANP32B.
ELVLDNC[+57.0]K
CPTAC-1596:
AP1B1.
LLSTDPVAAK
CPTAC-1597:
ARPC2.
VFMQEFK
CPTAC-1598:
BRD2.
LMFSNC[+57.0]YK
CPTAC-1599:
CDH1.
GLDARPEVTR
CPTAC-1600:
CBX3.
DSDEADLVLAK
CPTAC-1601:
CDC42.
NVFDEAILAALEPPEPK
CPTAC-1602:
CDK9.
IGQGTFGEVFK
CPTAC-1603:
C20orf27.
LLSVVPVPEGYSVK
CPTAC-1604:
DOCK2.
DILLPVITK
CPTAC-1605:
EHD2.
LEGHGLPANLPR
CPTAC-1606:
EML1.
VQMQEDDIQLLK
CPTAC-1607:
ENY2.
ALVPDSVK
CPTAC-1608:
FIGNL1.
GILLFGPPGTGK
CPTAC-1609:
GRB2.
ATADDELSFK
CPTAC-1610:
GTPBP1.
VGDNDFLEVR
CPTAC-1611:
C15orf38-AP3S2.
TGASWTDNIMAQK
CPTAC-1612:
HM13.
SFEAPIK
CPTAC-1613:
EPHX1.
DVELLYPVK
CPTAC-1614:
ITGA1.
FGTAIAAVK
CPTAC-1615:
PRKCA.
GTEELYAIK
CPTAC-1616:
LNPK.
IVEYLVGDGPQNR
CPTAC-1617:
LRP1.
AALSGANVLTLIEK
CPTAC-1618:
MCM2.
GLALALFGGEPK
CPTAC-1619:
MTA1.
YQADITDLLK
CPTAC-1620:
MYO1G.
TLVTLEQSR
CPTAC-1621:
NEK7.
VQIFDLMDAK
CPTAC-1622:
NF1.
LVFIDC[+57.0]PGK
CPTAC-1623:
FAM129A.
VLTSEDEYNLLSDR
CPTAC-1624:
NMRAL1.
LGFPGAR
CPTAC-1625:
OSBPL10.
LLEHGEEYVFTLPSAYAR
CPTAC-1626:
PIK3R1.
TATGYGFAEPYNLYSSLK
CPTAC-1627:
PACSIN2.
AIYHDLEQSIR
CPTAC-1628:
PAK2.
SVIDPVPAPVGDSHVDGAAK
CPTAC-1629:
PELP1.
TGSAVAPVHPPNR
CPTAC-1630:
PDGFRB.
LLGEVGTLQFAELHR
CPTAC-1631:
PIK3CA.
LINLTDILK
CPTAC-1632:
ARL6IP5.
AWDDFFPGSDR
CPTAC-1633:
RNF121.
GWC[+57.0]IVGK
CPTAC-1634:
POLR1E.
HFVGILNK
CPTAC-1635:
PDCD11.
VTPNEGLTVSFPFGK
CPTAC-1636:
SMARCA5.
TEQEEDEELLTESSK
CPTAC-1637:
SSBP1.
SGDSEVYQLGDVSQK
CPTAC-1638:
STAT5B.
ATQLLEGLVQELQK
CPTAC-1639:
STAT6.
SVSWSQFNK
CPTAC-1640:
STRN.
SELTDSASVLDNFK
CPTAC-1641:
SVIL.
YGSFEEAEASYPILNR
CPTAC-1642:
VARS.
NVIHPFLSR
CPTAC-1643:
CCT6A.
GIDPFSLDALSK
CPTAC-1644:
TGM2.
SEGTYC[+57.0]C[+57.0]GPVPVR
CPTAC-1645:
TIA1.
AAFAPFGR
CPTAC-1646:
TMEM43.
LEDPHVDIIR
CPTAC-1647:
TRA2A.
YGPLSGVNVVYDQR

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y4 (1+) 4.7 2.7 3.4 14.8 7 7.5 15.5 7.5 8.2 15 15 15
y5 (1+) 7.4 2.3 2.5 13.7 5.8 4.7 15.6 6.2 5.3 15 15 15
y6 (1+) 5 2.8 2.2 7.7 4 4.5 9.2 4.9 5 15 15 15
sum 3.5 1.7 2 10.9 5 5.1 11.4 5.3 5.5 15 15 15

Additional Resources and Comments