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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

EHD2

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
EHD2 PAST2
Sequence Length (AA) Molecular Weight (Da)
543 61161
Protein Name
EH domain-containing protein 2
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MFSWLKRGGA RGQQPEAIRT VTSALKELYR TKLLPLEEHY RFGAFHSPAL
60 70 80 90 100
EDADFDGKPM VLVAGQYSTG KTSFIQYLLE QEVPGSRVGP EPTTDCFVAV
110 120 130 140 150
MHGDTEGTVP GNALVVDPDK PFRKLNPFGN TFLNRFMCAQ LPNQVLESIS
160 170 180 190 200
IIDTPGILSG AKQRVSRGYD FPAVLRWFAE RVDLIILLFD AHKLEISDEF
210 220 230 240 250
SEAIGALRGH EDKIRVVLNK ADMVETQQLM RVYGALMWAL GKVVGTPEVL
260 270 280 290 300
RVYIGSFWSQ PLLVPDNRRL FELEEQDLFR DIQGLPRHAA LRKLNDLVKR
310 320 330 340 350
ARLVRVHAYI ISYLKKEMPS VFGKENKKKQ LILKLPVIFA KIQLEHHISP
360 370 380 390 400
GDFPDCQKMQ ELLMAHDFTK FHSLKPKLLE ALDEMLTHDI AKLMPLLRQE
410 420 430 440 450
ELESTEVGVQ GGAFEGTHMG PFVERGPDEA MEDGEEGSDD EAEWVVTKDK
460 470 480 490 500
SKYDEIFYNL APADGKLSGS KAKTWMVGTK LPNSVLGRIW KLSDVDRDGM
510 520 530 540 543
LDDEEFALAS HLIEAKLEGH GLPANLPRRL VPPSKRRHKG SAE

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1605 Collapse assay details

Data source: Panorama

Official Gene Symbol
EHD2
Peptide Sequence
LEGHGLPANLPR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
517
Peptide End
528
CPTAC ID
CPTAC-1605
Peptide Molecular Mass
1,272.6939
Species
Homo sapiens (Human)
Assay Type
Direct MRM or SRM
Matrix
Ovarian cancer tumor tissue lysate
Submitting Laboratory
Pacific Northwest National Laboratory
Submitting Lab PI
Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Waters nanoACQUITY (Part Number 176016000)
Column Packing
Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions
100 um x 100 mm
Flow Rate
0.5 uL/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-DirectSRM3

CPTAC-1593:
ABI2.
ALFDSYTNLER 		CPTAC-1594:
ALDH2.
VAEQTPLTALYVANLIK 		CPTAC-1595:
ANP32B.
ELVLDNC[+57.0]K
CPTAC-1596:
AP1B1.
LLSTDPVAAK 		CPTAC-1597:
ARPC2.
VFMQEFK 		CPTAC-1598:
BRD2.
LMFSNC[+57.0]YK
CPTAC-1599:
CDH1.
GLDARPEVTR 		CPTAC-1600:
CBX3.
DSDEADLVLAK 		CPTAC-1601:
CDC42.
NVFDEAILAALEPPEPK
CPTAC-1602:
CDK9.
IGQGTFGEVFK 		CPTAC-1603:
C20orf27.
LLSVVPVPEGYSVK 		CPTAC-1604:
DOCK2.
DILLPVITK
CPTAC-1605:
EHD2.
LEGHGLPANLPR 		CPTAC-1606:
EML1.
VQMQEDDIQLLK 		CPTAC-1607:
ENY2.
ALVPDSVK
CPTAC-1608:
FIGNL1.
GILLFGPPGTGK 		CPTAC-1609:
GRB2.
ATADDELSFK 		CPTAC-1610:
GTPBP1.
VGDNDFLEVR
CPTAC-1611:
C15orf38-AP3S2.
TGASWTDNIMAQK 		CPTAC-1612:
HM13.
SFEAPIK 		CPTAC-1613:
EPHX1.
DVELLYPVK
CPTAC-1614:
ITGA1.
FGTAIAAVK 		CPTAC-1615:
PRKCA.
GTEELYAIK 		CPTAC-1616:
LNPK.
IVEYLVGDGPQNR
CPTAC-1617:
LRP1.
AALSGANVLTLIEK 		CPTAC-1618:
MCM2.
GLALALFGGEPK 		CPTAC-1619:
MTA1.
YQADITDLLK
CPTAC-1620:
MYO1G.
TLVTLEQSR 		CPTAC-1621:
NEK7.
VQIFDLMDAK 		CPTAC-1622:
NF1.
LVFIDC[+57.0]PGK
CPTAC-1623:
FAM129A.
VLTSEDEYNLLSDR 		CPTAC-1624:
NMRAL1.
LGFPGAR 		CPTAC-1625:
OSBPL10.
LLEHGEEYVFTLPSAYAR
CPTAC-1626:
PIK3R1.
TATGYGFAEPYNLYSSLK 		CPTAC-1627:
PACSIN2.
AIYHDLEQSIR 		CPTAC-1628:
PAK2.
SVIDPVPAPVGDSHVDGAAK
CPTAC-1629:
PELP1.
TGSAVAPVHPPNR 		CPTAC-1630:
PDGFRB.
LLGEVGTLQFAELHR 		CPTAC-1631:
PIK3CA.
LINLTDILK
CPTAC-1632:
ARL6IP5.
AWDDFFPGSDR 		CPTAC-1633:
RNF121.
GWC[+57.0]IVGK 		CPTAC-1634:
POLR1E.
HFVGILNK
CPTAC-1635:
PDCD11.
VTPNEGLTVSFPFGK 		CPTAC-1636:
SMARCA5.
TEQEEDEELLTESSK 		CPTAC-1637:
SSBP1.
SGDSEVYQLGDVSQK
CPTAC-1638:
STAT5B.
ATQLLEGLVQELQK 		CPTAC-1639:
STAT6.
SVSWSQFNK 		CPTAC-1640:
STRN.
SELTDSASVLDNFK
CPTAC-1641:
SVIL.
YGSFEEAEASYPILNR 		CPTAC-1642:
VARS.
NVIHPFLSR 		CPTAC-1643:
CCT6A.
GIDPFSLDALSK
CPTAC-1644:
TGM2.
SEGTYC[+57.0]C[+57.0]GPVPVR 		CPTAC-1645:
TIA1.
AAFAPFGR 		CPTAC-1646:
TMEM43.
LEDPHVDIIR
CPTAC-1647:
TRA2A.
YGPLSGVNVVYDQR

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y4 (1+) 4.7 2.7 3.4 14.8 7 7.5 15.5 7.5 8.2 15 15 15
y5 (1+) 7.4 2.3 2.5 13.7 5.8 4.7 15.6 6.2 5.3 15 15 15
y6 (1+) 5 2.8 2.2 7.7 4 4.5 9.2 4.9 5 15 15 15
sum 3.5 1.7 2 10.9 5 5.1 11.4 5.3 5.5 15 15 15

Additional Resources and Comments

Resources
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View Peptide in Panorama
View Protein in Panorama
Comments