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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

POLR1E

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
POLR1E PAF53, PRAF1
Sequence Length (AA) Molecular Weight (Da)
481 53962
Protein Name
DNA-directed RNA polymerase I subunit RPA49
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MYQASAVSLL PRDIPSCHSP SPGFSHLPTS SSQLAPDLLQ FPLGQDPSFL
60 70 80 90 100
AIPILTLPPS DSLVPPYIVW YIVWPSALIS FLGCTLTVQF SNGKLQSPGN
110 120 130 140 150
MRFTLYENKD STNPRKRNQR ILAAETDRLS YVGNNFGTGA LKCNTLCRHF
160 170 180 190 200
VGILNKTSGQ MEVYDAELFN MQPLFSDVSV ESELALESQT KTYREKMDSC
210 220 230 240 250
IEAFGTTKQK RALNTRRMNR VGNESLNRAV AKAAETIIDT KGVTALVSDA
260 270 280 290 300
IHNDLQDDSL YLPPCYDDAA KPEDVYKFED LLSPAEYEAL QSPSEAFRNV
310 320 330 340 350
TSEEILKMIE ENSHCTFVIE ALKSLPSDVE SRDRQARCIW FLDTLIKFRA
360 370 380 390 400
HRVVKRKSAL GPGVPHIINT KLLKHFTCLT YNNGRLRNLI SDSMKAKITA
410 420 430 440 450
YVIILALHIH DFQIDLTVLQ RDLKLSEKRM MEIAKAMRLK ISKRRVSVAA
460 470 480 481
GSEEDHKLGT LSLPLPPAQT SDRLAKRRKI T

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1634 Collapse assay details

Data source: Panorama

Official Gene Symbol
POLR1E
Peptide Sequence
HFVGILNK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
149
Peptide End
156
CPTAC ID
CPTAC-1634
Peptide Molecular Mass
926.5338
Species
Homo sapiens (Human)
Assay Type
Direct MRM or SRM
Matrix
Ovarian cancer tumor tissue lysate
Submitting Laboratory
Pacific Northwest National Laboratory
Submitting Lab PI
Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Waters nanoACQUITY (Part Number 176016000)
Column Packing
Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions
100 um x 100 mm
Flow Rate
0.5 uL/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-DirectSRM3

CPTAC-1593:
ABI2.
ALFDSYTNLER 		CPTAC-1594:
ALDH2.
VAEQTPLTALYVANLIK 		CPTAC-1595:
ANP32B.
ELVLDNC[+57.0]K
CPTAC-1596:
AP1B1.
LLSTDPVAAK 		CPTAC-1597:
ARPC2.
VFMQEFK 		CPTAC-1598:
BRD2.
LMFSNC[+57.0]YK
CPTAC-1599:
CDH1.
GLDARPEVTR 		CPTAC-1600:
CBX3.
DSDEADLVLAK 		CPTAC-1601:
CDC42.
NVFDEAILAALEPPEPK
CPTAC-1602:
CDK9.
IGQGTFGEVFK 		CPTAC-1603:
C20orf27.
LLSVVPVPEGYSVK 		CPTAC-1604:
DOCK2.
DILLPVITK
CPTAC-1605:
EHD2.
LEGHGLPANLPR 		CPTAC-1606:
EML1.
VQMQEDDIQLLK 		CPTAC-1607:
ENY2.
ALVPDSVK
CPTAC-1608:
FIGNL1.
GILLFGPPGTGK 		CPTAC-1609:
GRB2.
ATADDELSFK 		CPTAC-1610:
GTPBP1.
VGDNDFLEVR
CPTAC-1611:
C15orf38-AP3S2.
TGASWTDNIMAQK 		CPTAC-1612:
HM13.
SFEAPIK 		CPTAC-1613:
EPHX1.
DVELLYPVK
CPTAC-1614:
ITGA1.
FGTAIAAVK 		CPTAC-1615:
PRKCA.
GTEELYAIK 		CPTAC-1616:
LNPK.
IVEYLVGDGPQNR
CPTAC-1617:
LRP1.
AALSGANVLTLIEK 		CPTAC-1618:
MCM2.
GLALALFGGEPK 		CPTAC-1619:
MTA1.
YQADITDLLK
CPTAC-1620:
MYO1G.
TLVTLEQSR 		CPTAC-1621:
NEK7.
VQIFDLMDAK 		CPTAC-1622:
NF1.
LVFIDC[+57.0]PGK
CPTAC-1623:
FAM129A.
VLTSEDEYNLLSDR 		CPTAC-1624:
NMRAL1.
LGFPGAR 		CPTAC-1625:
OSBPL10.
LLEHGEEYVFTLPSAYAR
CPTAC-1626:
PIK3R1.
TATGYGFAEPYNLYSSLK 		CPTAC-1627:
PACSIN2.
AIYHDLEQSIR 		CPTAC-1628:
PAK2.
SVIDPVPAPVGDSHVDGAAK
CPTAC-1629:
PELP1.
TGSAVAPVHPPNR 		CPTAC-1630:
PDGFRB.
LLGEVGTLQFAELHR 		CPTAC-1631:
PIK3CA.
LINLTDILK
CPTAC-1632:
ARL6IP5.
AWDDFFPGSDR 		CPTAC-1633:
RNF121.
GWC[+57.0]IVGK 		CPTAC-1634:
POLR1E.
HFVGILNK
CPTAC-1635:
PDCD11.
VTPNEGLTVSFPFGK 		CPTAC-1636:
SMARCA5.
TEQEEDEELLTESSK 		CPTAC-1637:
SSBP1.
SGDSEVYQLGDVSQK
CPTAC-1638:
STAT5B.
ATQLLEGLVQELQK 		CPTAC-1639:
STAT6.
SVSWSQFNK 		CPTAC-1640:
STRN.
SELTDSASVLDNFK
CPTAC-1641:
SVIL.
YGSFEEAEASYPILNR 		CPTAC-1642:
VARS.
NVIHPFLSR 		CPTAC-1643:
CCT6A.
GIDPFSLDALSK
CPTAC-1644:
TGM2.
SEGTYC[+57.0]C[+57.0]GPVPVR 		CPTAC-1645:
TIA1.
AAFAPFGR 		CPTAC-1646:
TMEM43.
LEDPHVDIIR
CPTAC-1647:
TRA2A.
YGPLSGVNVVYDQR

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b6 (1+) 9.6 5.9 4.3 15.5 9.1 5.9 18.2 10.8 7.3 15 15 15
b5 (1+) 8.9 6.8 5.9 16.4 12.8 12.4 18.7 14.5 13.7 15 15 15
y7 (1+) 6.2 2.6 4.1 10.7 4.2 5.3 12.4 4.9 6.7 15 15 15
sum 5.2 2.2 2.9 11.3 5.7 6.2 12.4 6.1 6.8 15 15 15

Additional Resources and Comments

Resources
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View Peptide in Panorama
View Protein in Panorama
Comments