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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

PDCD11

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
PDCD11 KIAA0185
Sequence Length (AA) Molecular Weight (Da)
1871 208701
Protein Name
Protein RRP5 homolog
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MANLEESFPR GGTRKIHKPE KAFQQSVEQD NLFDISTEEG STKRKKSQKG
60 70 80 90 100
PAKTKKLKIE KRESSKSARE KFEILSVESL CEGMRILGCV KEVNELELVI
110 120 130 140 150
SLPNGLQGFV QVTEICDAYT KKLNEQVTQE QPLKDLLHLP ELFSPGMLVR
160 170 180 190 200
CVVSSLGITD RGKKSVKLSL NPKNVNRVLS AEALKPGMLL TGTVSSLEDH
210 220 230 240 250
GYLVDIGVDG TRAFLPLLKA QEYIRQKNKG AKLKVGQYLN CIVEKVKGNG
260 270 280 290 300
GVVSLSVGHS EVSTAIATEQ QSWNLNNLLP GLVVKAQVQK VTPFGLTLNF
310 320 330 340 350
LTFFTGVVDF MHLDPKKAGT YFSNQAVRAC ILCVHPRTRV VHLSLRPIFL
360 370 380 390 400
QPGRPLTRLS CQNLGAVLDD VPVQGFFKKA GATFRLKDGV LAYARLSHLS
410 420 430 440 450
DSKNVFNPEA FKPGNTHKCR IIDYSQMDEL ALLSLRTSII EAQYLRYHDI
460 470 480 490 500
EPGAVVKGTV LTIKSYGMLV KVGEQMRGLV PPMHLADILM KNPEKKYHIG
510 520 530 540 550
DEVKCRVLLC DPEAKKLMMT LKKTLIESKL PVITCYADAK PGLQTHGFII
560 570 580 590 600
RVKDYGCIVK FYNNVQGLVP KHELSTEYIP DPERVFYTGQ VVKVVVLNCE
610 620 630 640 650
PSKERMLLSF KLSSDPEPKK EPAGHSQKKG KAINIGQLVD VKVLEKTKDG
660 670 680 690 700
LEVAVLPHNI RAFLPTSHLS DHVANGPLLH HWLQAGDILH RVLCLSQSEG
710 720 730 740 750
RVLLCRKPAL VSTVEGGQDP KNFSEIHPGM LLIGFVKSIK DYGVFIQFPS
760 770 780 790 800
GLSGLAPKAI MSDKFVTSTS DHFVEGQTVA AKVTNVDEEK QRMLLSLRLS
810 820 830 840 850
DCGLGDLAIT SLLLLNQCLE ELQGVRSLMS NRDSVLIQTL AEMTPGMFLD
860 870 880 890 900
LVVQEVLEDG SVVFSGGPVP DLVLKASRYH RAGQEVESGQ KKKVVILNVD
910 920 930 940 950
LLKLEVHVSL HQDLVNRKAR KLRKGSEHQA IVQHLEKSFA IASLVETGHL
960 970 980 990 1000
AAFSLTSHLN DTFRFDSEKL QVGQGVSLTL KTTEPGVTGL LLAVEGPAAK
1010 1020 1030 1040 1050
RTMRPTQKDS ETVDEDEEVD PALTVGTIKK HTLSIGDMVT GTVKSIKPTH
1060 1070 1080 1090 1100
VVVTLEDGII GCIHASHILD DVPEGTSPTT KLKVGKTVTA RVIGGRDMKT
1110 1120 1130 1140 1150
FKYLPISHPR FVRTIPELSV RPSELEDGHT ALNTHSVSPM EKIKQYQAGQ
1160 1170 1180 1190 1200
TVTCFLKKYN VVKKWLEVEI APDIRGRIPL LLTSLSFKVL KHPDKKFRVG
1210 1220 1230 1240 1250
QALRATVVGP DSSKTLLCLS LTGPHKLEEG EVAMGRVVKV TPNEGLTVSF
1260 1270 1280 1290 1300
PFGKIGTVSI FHMSDSYSET PLEDFVPQKV VRCYILSTAD NVLTLSLRSS
1310 1320 1330 1340 1350
RTNPETKSKV EDPEINSIQD IKEGQLLRGY VGSIQPHGVF FRLGPSVVGL
1360 1370 1380 1390 1400
ARYSHVSQHS PSKKALYNKH LPEGKLLTAR VLRLNHQKNL VELSFLPGDT
1410 1420 1430 1440 1450
GKPDVLSASL EGQLTKQEER KTEAEERDQK GEKKNQKRNE KKNQKGQEEV
1460 1470 1480 1490 1500
EMPSKEKQQP QKPQAQKRGG RECRESGSEQ ERVSKKPKKA GLSEEDDSLV
1510 1520 1530 1540 1550
DVYYREGKEE AEETNVLPKE KQTKPAEAPR LQLSSGFAWN VGLDSLTPAL
1560 1570 1580 1590 1600
PPLAESSDSE EDEKPHQATI KKSKKERELE KQKAEKELSR IEEALMDPGR
1610 1620 1630 1640 1650
QPESADDFDR LVLSSPNSSI LWLQYMAFHL QATEIEKARA VAERALKTIS
1660 1670 1680 1690 1700
FREEQEKLNV WVALLNLENM YGSQESLTKV FERAVQYNEP LKVFLHLADI
1710 1720 1730 1740 1750
YAKSEKFQEA GELYNRMLKR FRQEKAVWIK YGAFLLRRSQ AAASHRVLQR
1760 1770 1780 1790 1800
ALECLPSKEH VDVIAKFAQL EFQLGDAERA KAIFENTLST YPKRTDVWSV
1810 1820 1830 1840 1850
YIDMTIKHGS QKDVRDIFER VIHLSLAPKR MKFFFKRYLD YEKQHGTEKD
1860 1870 1871
VQAVKAKALE YVEAKSSVLE D

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1635 Collapse assay details

Data source: Panorama

Official Gene Symbol
PDCD11
Peptide Sequence
VTPNEGLTVSFPFGK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
1240
Peptide End
1254
CPTAC ID
CPTAC-1635
Peptide Molecular Mass
1,591.8246
Species
Homo sapiens (Human)
Assay Type
Direct MRM or SRM
Matrix
Ovarian cancer tumor tissue lysate
Submitting Laboratory
Pacific Northwest National Laboratory
Submitting Lab PI
Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Waters nanoACQUITY (Part Number 176016000)
Column Packing
Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions
100 um x 100 mm
Flow Rate
0.5 uL/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-DirectSRM3

CPTAC-1593:
ABI2.
ALFDSYTNLER 		CPTAC-1594:
ALDH2.
VAEQTPLTALYVANLIK 		CPTAC-1595:
ANP32B.
ELVLDNC[+57.0]K
CPTAC-1596:
AP1B1.
LLSTDPVAAK 		CPTAC-1597:
ARPC2.
VFMQEFK 		CPTAC-1598:
BRD2.
LMFSNC[+57.0]YK
CPTAC-1599:
CDH1.
GLDARPEVTR 		CPTAC-1600:
CBX3.
DSDEADLVLAK 		CPTAC-1601:
CDC42.
NVFDEAILAALEPPEPK
CPTAC-1602:
CDK9.
IGQGTFGEVFK 		CPTAC-1603:
C20orf27.
LLSVVPVPEGYSVK 		CPTAC-1604:
DOCK2.
DILLPVITK
CPTAC-1605:
EHD2.
LEGHGLPANLPR 		CPTAC-1606:
EML1.
VQMQEDDIQLLK 		CPTAC-1607:
ENY2.
ALVPDSVK
CPTAC-1608:
FIGNL1.
GILLFGPPGTGK 		CPTAC-1609:
GRB2.
ATADDELSFK 		CPTAC-1610:
GTPBP1.
VGDNDFLEVR
CPTAC-1611:
C15orf38-AP3S2.
TGASWTDNIMAQK 		CPTAC-1612:
HM13.
SFEAPIK 		CPTAC-1613:
EPHX1.
DVELLYPVK
CPTAC-1614:
ITGA1.
FGTAIAAVK 		CPTAC-1615:
PRKCA.
GTEELYAIK 		CPTAC-1616:
LNPK.
IVEYLVGDGPQNR
CPTAC-1617:
LRP1.
AALSGANVLTLIEK 		CPTAC-1618:
MCM2.
GLALALFGGEPK 		CPTAC-1619:
MTA1.
YQADITDLLK
CPTAC-1620:
MYO1G.
TLVTLEQSR 		CPTAC-1621:
NEK7.
VQIFDLMDAK 		CPTAC-1622:
NF1.
LVFIDC[+57.0]PGK
CPTAC-1623:
FAM129A.
VLTSEDEYNLLSDR 		CPTAC-1624:
NMRAL1.
LGFPGAR 		CPTAC-1625:
OSBPL10.
LLEHGEEYVFTLPSAYAR
CPTAC-1626:
PIK3R1.
TATGYGFAEPYNLYSSLK 		CPTAC-1627:
PACSIN2.
AIYHDLEQSIR 		CPTAC-1628:
PAK2.
SVIDPVPAPVGDSHVDGAAK
CPTAC-1629:
PELP1.
TGSAVAPVHPPNR 		CPTAC-1630:
PDGFRB.
LLGEVGTLQFAELHR 		CPTAC-1631:
PIK3CA.
LINLTDILK
CPTAC-1632:
ARL6IP5.
AWDDFFPGSDR 		CPTAC-1633:
RNF121.
GWC[+57.0]IVGK 		CPTAC-1634:
POLR1E.
HFVGILNK
CPTAC-1635:
PDCD11.
VTPNEGLTVSFPFGK 		CPTAC-1636:
SMARCA5.
TEQEEDEELLTESSK 		CPTAC-1637:
SSBP1.
SGDSEVYQLGDVSQK
CPTAC-1638:
STAT5B.
ATQLLEGLVQELQK 		CPTAC-1639:
STAT6.
SVSWSQFNK 		CPTAC-1640:
STRN.
SELTDSASVLDNFK
CPTAC-1641:
SVIL.
YGSFEEAEASYPILNR 		CPTAC-1642:
VARS.
NVIHPFLSR 		CPTAC-1643:
CCT6A.
GIDPFSLDALSK
CPTAC-1644:
TGM2.
SEGTYC[+57.0]C[+57.0]GPVPVR 		CPTAC-1645:
TIA1.
AAFAPFGR 		CPTAC-1646:
TMEM43.
LEDPHVDIIR
CPTAC-1647:
TRA2A.
YGPLSGVNVVYDQR

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y13 (2+) 5 2.3 3.7 15.5 5.7 6.3 16.3 6.1 7.3 15 15 15
y8 (1+) 18.4 3.3 4.4 26.8 9.1 5.4 32.5 9.7 7 15 15 15
y10 (1+) 17 5.9 7 22.6 7 7.6 28.3 9.2 10.3 15 15 15
sum 8.1 2.1 3.3 16.3 5.9 5.4 18.2 6.3 6.3 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
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