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Overview Data source: UniProt

Official Gene Symbol Other Aliases
SMARCA5 SNF2H, WCRF135
Sequence Length (AA) Molecular Weight (Da)
1052 121905
Protein Name
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 5
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MSSAAEPPPP PPPESAPSKP AASIASGGSN SSNKGGPEGV AAQAVASAAS
60 70 80 90 100
AGPADAEMEE IFDDASPGKQ KEIQEPDPTY EEKMQTDRAN RFEYLLKQTE
110 120 130 140 150
LFAHFIQPAA QKTPTSPLKM KPGRPRIKKD EKQNLLSVGD YRHRRTEQEE
160 170 180 190 200
DEELLTESSK ATNVCTRFED SPSYVKWGKL RDYQVRGLNW LISLYENGIN
210 220 230 240 250
GILADEMGLG KTLQTISLLG YMKHYRNIPG PHMVLVPKST LHNWMSEFKR
260 270 280 290 300
WVPTLRSVCL IGDKEQRAAF VRDVLLPGEW DVCVTSYEML IKEKSVFKKF
310 320 330 340 350
NWRYLVIDEA HRIKNEKSKL SEIVREFKTT NRLLLTGTPL QNNLHELWSL
360 370 380 390 400
LNFLLPDVFN SADDFDSWFD TNNCLGDQKL VERLHMVLRP FLLRRIKADV
410 420 430 440 450
EKSLPPKKEV KIYVGLSKMQ REWYTRILMK DIDILNSAGK MDKMRLLNIL
460 470 480 490 500
MQLRKCCNHP YLFDGAEPGP PYTTDMHLVT NSGKMVVLDK LLPKLKEQGS
510 520 530 540 550
RVLIFSQMTR VLDILEDYCM WRNYEYCRLD GQTPHDERQD SINAYNEPNS
560 570 580 590 600
TKFVFMLSTR AGGLGINLAT ADVVILYDSD WNPQVDLQAM DRAHRIGQTK
610 620 630 640 650
TVRVFRFITD NTVEERIVER AEMKLRLDSI VIQQGRLVDQ NLNKIGKDEM
660 670 680 690 700
LQMIRHGATH VFASKESEIT DEDIDGILER GAKKTAEMNE KLSKMGESSL
710 720 730 740 750
RNFTMDTESS VYNFEGEDYR EKQKIAFTEW IEPPKRERKA NYAVDAYFRE
760 770 780 790 800
ALRVSEPKAP KAPRPPKQPN VQDFQFFPPR LFELLEKEIL FYRKTIGYKV
810 820 830 840 850
PRNPELPNAA QAQKEEQLKI DEAESLNDEE LEEKEKLLTQ GFTNWNKRDF
860 870 880 890 900
NQFIKANEKW GRDDIENIAR EVEGKTPEEV IEYSAVFWER CNELQDIEKI
910 920 930 940 950
MAQIERGEAR IQRRISIKKA LDTKIGRYKA PFHQLRISYG TNKGKNYTEE
960 970 980 990 1000
EDRFLICMLH KLGFDKENVY DELRQCIRNS PQFRFDWFLK SRTAMELQRR
1010 1020 1030 1040 1050
CNTLITLIER ENMELEEKEK AEKKKRGPKP STQKRKMDGA PDGRGRKKKL
1052
KL

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1636 Collapse assay details

Data source: Panorama

Official Gene Symbol
SMARCA5
Peptide Sequence
TEQEEDEELLTESSK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
146
Peptide End
160
CPTAC ID
CPTAC-1636
Peptide Molecular Mass
1,765.7741
Species
Homo sapiens (Human)
Assay Type
Direct MRM or SRM
Matrix
Ovarian cancer tumor tissue lysate
Submitting Laboratory
Pacific Northwest National Laboratory
Submitting Lab PI
Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Waters nanoACQUITY (Part Number 176016000)
Column Packing
Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions
100 um x 100 mm
Flow Rate
0.5 uL/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-DirectSRM3

CPTAC-1593:
ABI2.
ALFDSYTNLER
CPTAC-1594:
ALDH2.
VAEQTPLTALYVANLIK
CPTAC-1595:
ANP32B.
ELVLDNC[+57.0]K
CPTAC-1596:
AP1B1.
LLSTDPVAAK
CPTAC-1597:
ARPC2.
VFMQEFK
CPTAC-1598:
BRD2.
LMFSNC[+57.0]YK
CPTAC-1599:
CDH1.
GLDARPEVTR
CPTAC-1600:
CBX3.
DSDEADLVLAK
CPTAC-1601:
CDC42.
NVFDEAILAALEPPEPK
CPTAC-1602:
CDK9.
IGQGTFGEVFK
CPTAC-1603:
C20orf27.
LLSVVPVPEGYSVK
CPTAC-1604:
DOCK2.
DILLPVITK
CPTAC-1605:
EHD2.
LEGHGLPANLPR
CPTAC-1606:
EML1.
VQMQEDDIQLLK
CPTAC-1607:
ENY2.
ALVPDSVK
CPTAC-1608:
FIGNL1.
GILLFGPPGTGK
CPTAC-1609:
GRB2.
ATADDELSFK
CPTAC-1610:
GTPBP1.
VGDNDFLEVR
CPTAC-1611:
C15orf38-AP3S2.
TGASWTDNIMAQK
CPTAC-1612:
HM13.
SFEAPIK
CPTAC-1613:
EPHX1.
DVELLYPVK
CPTAC-1614:
ITGA1.
FGTAIAAVK
CPTAC-1615:
PRKCA.
GTEELYAIK
CPTAC-1616:
LNPK.
IVEYLVGDGPQNR
CPTAC-1617:
LRP1.
AALSGANVLTLIEK
CPTAC-1618:
MCM2.
GLALALFGGEPK
CPTAC-1619:
MTA1.
YQADITDLLK
CPTAC-1620:
MYO1G.
TLVTLEQSR
CPTAC-1621:
NEK7.
VQIFDLMDAK
CPTAC-1622:
NF1.
LVFIDC[+57.0]PGK
CPTAC-1623:
FAM129A.
VLTSEDEYNLLSDR
CPTAC-1624:
NMRAL1.
LGFPGAR
CPTAC-1625:
OSBPL10.
LLEHGEEYVFTLPSAYAR
CPTAC-1626:
PIK3R1.
TATGYGFAEPYNLYSSLK
CPTAC-1627:
PACSIN2.
AIYHDLEQSIR
CPTAC-1628:
PAK2.
SVIDPVPAPVGDSHVDGAAK
CPTAC-1629:
PELP1.
TGSAVAPVHPPNR
CPTAC-1630:
PDGFRB.
LLGEVGTLQFAELHR
CPTAC-1631:
PIK3CA.
LINLTDILK
CPTAC-1632:
ARL6IP5.
AWDDFFPGSDR
CPTAC-1633:
RNF121.
GWC[+57.0]IVGK
CPTAC-1634:
POLR1E.
HFVGILNK
CPTAC-1635:
PDCD11.
VTPNEGLTVSFPFGK
CPTAC-1636:
SMARCA5.
TEQEEDEELLTESSK
CPTAC-1637:
SSBP1.
SGDSEVYQLGDVSQK
CPTAC-1638:
STAT5B.
ATQLLEGLVQELQK
CPTAC-1639:
STAT6.
SVSWSQFNK
CPTAC-1640:
STRN.
SELTDSASVLDNFK
CPTAC-1641:
SVIL.
YGSFEEAEASYPILNR
CPTAC-1642:
VARS.
NVIHPFLSR
CPTAC-1643:
CCT6A.
GIDPFSLDALSK
CPTAC-1644:
TGM2.
SEGTYC[+57.0]C[+57.0]GPVPVR
CPTAC-1645:
TIA1.
AAFAPFGR
CPTAC-1646:
TMEM43.
LEDPHVDIIR
CPTAC-1647:
TRA2A.
YGPLSGVNVVYDQR

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (1+) 10 5.5 7.5 19.9 5.8 12.9 22.3 8 14.9 15 15 15
y11 (1+) 27.1 12.5 10.1 25.9 20.3 12.3 37.5 23.8 15.9 15 15 15
y12 (1+) 23.1 10.1 11.3 32.2 12.6 19.2 39.6 16.1 22.3 15 15 15
sum 3.7 4.9 5.3 15.5 4.2 8 15.9 6.5 9.6 15 15 15

Additional Resources and Comments