Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
ANXA7 ANX7, SNX
Sequence Length (AA) Molecular Weight (Da)
488 52739
Protein Name
Annexin A7
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MSYPGYPPTG YPPFPGYPPA GQESSFPPSG QYPYPSGFPP MGGGAYPQVP
60 70 80 90 100
SSGYPGAGGY PAPGGYPAPG GYPGAPQPGG APSYPGVPPG QGFGVPPGGA
110 120 130 140 150
GFSGYPQPPS QSYGGGPAQV PLPGGFPGGQ MPSQYPGGQP TYPSQINTDS
160 170 180 190 200
FSSYPVFSPV SLDYSSEPAT VTQVTQGTIR PAANFDAIRD AEILRKAMKG
210 220 230 240 250
FGTDEQAIVD VVANRSNDQR QKIKAAFKTS YGKDLIKDLK SELSGNMEEL
260 270 280 290 300
ILALFMPPTY YDAWSLRKAM QGAGTQERVL IEILCTRTNQ EIREIVRCYQ
310 320 330 340 350
SEFGRDLEKD IRSDTSGHFE RLLVSMCQGN RDENQSINHQ MAQEDAQRLY
360 370 380 390 400
QAGEGRLGTD ESCFNMILAT RSFPQLRATM EAYSRMANRD LLSSVSREFS
410 420 430 440 450
GYVESGLKTI LQCALNRPAF FAERLYYAMK GAGTDDSTLV RIVVTRSEID
460 470 480 488
LVQIKQMFAQ MYQKTLGTMI AGDTSGDYRR LLLAIVGQ

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node
to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1388 Collapse assay details

Data source: Panorama

Official Gene Symbol
ANXA7
Peptide Sequence
EFSGYVESGLK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
398
Peptide End
408
CPTAC ID
CPTAC-1388
Peptide Molecular Mass
1,214.5819
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
depleted digested plasma
Submitting Laboratory
Broad Institute
Submitting Lab PI
Steven A. Carr

Publication

View Details (opens in a new window)

Large-Scale Interlaboratory Study to Develop, Analytically Validate and Apply Highly Multiplexed, Quantitative Peptide Assays to Measure Cancer-Relevant Proteins in Plasma. Abbatiello SE, Schilling B, Mani DR, Zimmerman LJ, Hall SC, MacLean B, Albertolle M, Allen S, Burgess M, Cusack MP, Gosh M, Hedrick V, Held JM, Inerowicz HD, Jackson A, Keshishian H, Kinsinger CR, Lyssand J, Makowski L, Mesri M, Rodriguez H, Rudnick P, Sadowski P, Sedransk N, Shaddox K, Skates SJ, Kuhn E, Smith D, Whiteaker JR, Whitwell C, Zhang S, Borchers CH, Fisher SJ, Gibson BW, Liebler DC, MacCoss MJ, Neubert TA, Paulovich AG, Regnier FE, Tempst P, Carr SA. Mol Cell Proteomics. 2015 Sep;14(9):2357-74. doi: 10.1074/mcp.M114.047050. Epub 2015 Feb 18. PMID: 25693799


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Quantiva TSQ
Internal Standard
heavy stable isotope peptide (delta 8-10)
Peptide Standard Purity
>95%
Peptide Standard Label Type
15N
LC
Easy NanoLC1000
Column Packing
Reprosil C18, 1.9um, 200A
Column Dimensions
0.075 x 150 mm
Flow Rate
200 nL/min

Assay Multiplexing Expand assay panel

Broad Institute-Plasma-based Candidate Cancer Biomarkers


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y9 (1+) 4.7 2.9 2.7 5.2 6.5 4.8 7 7.1 5.5 15 15 15
y6 (1+) 2.4 2.7 2 8.6 6.5 4.4 8.9 7 4.8 15 15 15
y5 (1+) 7.2 2 2.4 10.3 6.3 3.6 12.6 6.6 4.3 15 15 15
sum 2.7 2 1.9 7.5 6.3 4 8 6.6 4.4 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-1387 Collapse assay details

Data source: Panorama

Official Gene Symbol
ANXA7
Peptide Sequence
GAGTDDSTLVR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
431
Peptide End
441
CPTAC ID
CPTAC-1387
Peptide Molecular Mass
1,090.5255
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
depleted digested plasma
Submitting Laboratory
Broad Institute
Submitting Lab PI
Steven A. Carr

Publication

View Details (opens in a new window)

Large-Scale Interlaboratory Study to Develop, Analytically Validate and Apply Highly Multiplexed, Quantitative Peptide Assays to Measure Cancer-Relevant Proteins in Plasma. Abbatiello SE, Schilling B, Mani DR, Zimmerman LJ, Hall SC, MacLean B, Albertolle M, Allen S, Burgess M, Cusack MP, Gosh M, Hedrick V, Held JM, Inerowicz HD, Jackson A, Keshishian H, Kinsinger CR, Lyssand J, Makowski L, Mesri M, Rodriguez H, Rudnick P, Sadowski P, Sedransk N, Shaddox K, Skates SJ, Kuhn E, Smith D, Whiteaker JR, Whitwell C, Zhang S, Borchers CH, Fisher SJ, Gibson BW, Liebler DC, MacCoss MJ, Neubert TA, Paulovich AG, Regnier FE, Tempst P, Carr SA. Mol Cell Proteomics. 2015 Sep;14(9):2357-74. doi: 10.1074/mcp.M114.047050. Epub 2015 Feb 18. PMID: 25693799


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Quantiva TSQ
Internal Standard
heavy stable isotope peptide (delta 8-10)
Peptide Standard Purity
>95%
Peptide Standard Label Type
15N
LC
Easy NanoLC1000
Column Packing
Reprosil C18, 1.9um, 200A
Column Dimensions
0.075 x 150 mm
Flow Rate
200 nL/min

Assay Multiplexing Expand assay panel

Broad Institute-Plasma-based Candidate Cancer Biomarkers


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 3 3.1 1.7 3.1 5.9 2.7 4.3 6.7 3.2 15 15 15
y6 (1+) 3.8 2.7 2.7 4.5 5.7 3 5.9 6.3 4 15 15 15
y5 (1+) 2.5 3.2 2.5 3.7 5.9 4.1 4.5 6.7 4.8 15 15 15
sum 2.2 2.6 2.1 3.1 5.7 3.2 3.8 6.3 3.8 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-1389 Collapse assay details

Data source: Panorama

Official Gene Symbol
ANXA7
Peptide Sequence
GFGTDEQAIVDVVANR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
200
Peptide End
215
CPTAC ID
CPTAC-1389
Peptide Molecular Mass
1,689.8322
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
depleted digested plasma
Submitting Laboratory
Broad Institute
Submitting Lab PI
Steven A. Carr

Publication

View Details (opens in a new window)

Large-Scale Interlaboratory Study to Develop, Analytically Validate and Apply Highly Multiplexed, Quantitative Peptide Assays to Measure Cancer-Relevant Proteins in Plasma. Abbatiello SE, Schilling B, Mani DR, Zimmerman LJ, Hall SC, MacLean B, Albertolle M, Allen S, Burgess M, Cusack MP, Gosh M, Hedrick V, Held JM, Inerowicz HD, Jackson A, Keshishian H, Kinsinger CR, Lyssand J, Makowski L, Mesri M, Rodriguez H, Rudnick P, Sadowski P, Sedransk N, Shaddox K, Skates SJ, Kuhn E, Smith D, Whiteaker JR, Whitwell C, Zhang S, Borchers CH, Fisher SJ, Gibson BW, Liebler DC, MacCoss MJ, Neubert TA, Paulovich AG, Regnier FE, Tempst P, Carr SA. Mol Cell Proteomics. 2015 Sep;14(9):2357-74. doi: 10.1074/mcp.M114.047050. Epub 2015 Feb 18. PMID: 25693799


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Quantiva TSQ
Internal Standard
heavy stable isotope peptide (delta 8-10)
Peptide Standard Purity
>95%
Peptide Standard Label Type
15N
LC
Easy NanoLC1000
Column Packing
Reprosil C18, 1.9um, 200A
Column Dimensions
0.075 x 150 mm
Flow Rate
200 nL/min

Assay Multiplexing Expand assay panel

Broad Institute-Plasma-based Candidate Cancer Biomarkers


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y9 (1+) 6.1 5.2 5.3 14.1 9.6 5.2 15.4 10.9 7.4 15 15 15
y8 (1+) 9.4 8 7.7 13.7 10.5 9.9 16.6 13.2 12.5 15 15 15
y7 (1+) 7.7 5.7 2.8 17.2 9.6 3.6 18.8 11.2 4.6 15 15 15
sum 5 3.6 4.2 13.6 9 5.2 14.5 9.7 6.7 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-1385 Collapse assay details

Data source: Panorama

Official Gene Symbol
ANXA7
Peptide Sequence
LYQAGEGR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
349
Peptide End
356
CPTAC ID
CPTAC-1385
Peptide Molecular Mass
892.4403
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
depleted digested plasma
Submitting Laboratory
Broad Institute
Submitting Lab PI
Steven A. Carr

Publication

View Details (opens in a new window)

Large-Scale Interlaboratory Study to Develop, Analytically Validate and Apply Highly Multiplexed, Quantitative Peptide Assays to Measure Cancer-Relevant Proteins in Plasma. Abbatiello SE, Schilling B, Mani DR, Zimmerman LJ, Hall SC, MacLean B, Albertolle M, Allen S, Burgess M, Cusack MP, Gosh M, Hedrick V, Held JM, Inerowicz HD, Jackson A, Keshishian H, Kinsinger CR, Lyssand J, Makowski L, Mesri M, Rodriguez H, Rudnick P, Sadowski P, Sedransk N, Shaddox K, Skates SJ, Kuhn E, Smith D, Whiteaker JR, Whitwell C, Zhang S, Borchers CH, Fisher SJ, Gibson BW, Liebler DC, MacCoss MJ, Neubert TA, Paulovich AG, Regnier FE, Tempst P, Carr SA. Mol Cell Proteomics. 2015 Sep;14(9):2357-74. doi: 10.1074/mcp.M114.047050. Epub 2015 Feb 18. PMID: 25693799


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Quantiva TSQ
Internal Standard
heavy stable isotope peptide (delta 8-10)
Peptide Standard Purity
>95%
Peptide Standard Label Type
15N
LC
Easy NanoLC1000
Column Packing
Reprosil C18, 1.9um, 200A
Column Dimensions
0.075 x 150 mm
Flow Rate
200 nL/min

Assay Multiplexing Expand assay panel

Broad Institute-Plasma-based Candidate Cancer Biomarkers


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (1+) 2.2 2.3 3.3 4.9 7.4 4.5 5.4 7.7 5.6 15 15 15
y7 (1+) 6.7 3.8 3.6 7.9 8.1 4.4 10.4 8.9 5.7 15 15 15
y6 (1+) 3.8 2.6 2.4 4.8 8.2 3.8 6.1 8.6 4.5 15 15 15
sum 2.8 2.5 2.6 4.3 7.8 3.9 5.1 8.2 4.7 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-1386 Collapse assay details

Data source: Panorama

Official Gene Symbol
ANXA7
Peptide Sequence
SEIDLVQIK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
447
Peptide End
455
CPTAC ID
CPTAC-1386
Peptide Molecular Mass
1,043.5863
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
depleted digested plasma
Submitting Laboratory
Broad Institute
Submitting Lab PI
Steven A. Carr

Publication

View Details (opens in a new window)

Large-Scale Interlaboratory Study to Develop, Analytically Validate and Apply Highly Multiplexed, Quantitative Peptide Assays to Measure Cancer-Relevant Proteins in Plasma. Abbatiello SE, Schilling B, Mani DR, Zimmerman LJ, Hall SC, MacLean B, Albertolle M, Allen S, Burgess M, Cusack MP, Gosh M, Hedrick V, Held JM, Inerowicz HD, Jackson A, Keshishian H, Kinsinger CR, Lyssand J, Makowski L, Mesri M, Rodriguez H, Rudnick P, Sadowski P, Sedransk N, Shaddox K, Skates SJ, Kuhn E, Smith D, Whiteaker JR, Whitwell C, Zhang S, Borchers CH, Fisher SJ, Gibson BW, Liebler DC, MacCoss MJ, Neubert TA, Paulovich AG, Regnier FE, Tempst P, Carr SA. Mol Cell Proteomics. 2015 Sep;14(9):2357-74. doi: 10.1074/mcp.M114.047050. Epub 2015 Feb 18. PMID: 25693799


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Quantiva TSQ
Internal Standard
heavy stable isotope peptide (delta 8-10)
Peptide Standard Purity
>95%
Peptide Standard Label Type
15N
LC
Easy NanoLC1000
Column Packing
Reprosil C18, 1.9um, 200A
Column Dimensions
0.075 x 150 mm
Flow Rate
200 nL/min

Assay Multiplexing Expand assay panel

Broad Institute-Plasma-based Candidate Cancer Biomarkers


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 2.7 2.5 3.4 6.2 6.9 4.3 6.8 7.3 5.5 15 15 15
y6 (1+) 3.4 2 2.8 7.8 5.9 4.2 8.5 6.2 5 15 15 15
y5 (1+) 4.9 3.5 2.9 7.8 6.1 4 9.2 7 4.9 15 15 15
sum 2.4 2 2.6 6.7 5.9 3.9 7.1 6.2 4.7 15 15 15


Additional Resources and Comments


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