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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
PDLIM1 CLIM1, CLP36
Sequence Length (AA) Molecular Weight (Da)
329 36072
Protein Name
PDZ and LIM domain protein 1
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MTTQQIDLQG PGPWGFRLVG GKDFEQPLAI SRVTPGSKAA LANLCIGDVI
60 70 80 90 100
TAIDGENTSN MTHLEAQNRI KGCTDNLTLT VARSEHKVWS PLVTEEGKRH
110 120 130 140 150
PYKMNLASEP QEVLHIGSAH NRSAMPFTAS PASSTTARVI TNQYNNPAGL
160 170 180 190 200
YSSENISNFN NALESKTAAS GVEANSRPLD HAQPPSSLVI DKESEVYKML
210 220 230 240 250
QEKQELNEPP KQSTSFLVLQ EILESEEKGD PNKPSGFRSV KAPVTKVAAS
260 270 280 290 300
IGNAQKLPMC DKCGTGIVGV FVKLRDRHRH PECYVCTDCG TNLKQKGHFF
310 320 329
VEDQIYCEKH ARERVTPPEG YEVVTVFPK

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node
to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1441 Collapse assay details

Data source: Panorama

Official Gene Symbol
PDLIM1
Peptide Modified Sequence
C[+57.0]GTGIVGVFVK
Modification Type
Carbamidomethyl Cysteine
Protein - Site of Modification
263
Peptide - Site of Modification
1
Peptide Start
263
Peptide End
273
CPTAC ID
CPTAC-1441
Peptide Molecular Mass
1,135.6060
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
depleted digested plasma
Submitting Laboratory
Broad Institute
Submitting Lab PI
Steven A. Carr

Publication

View Details (opens in a new window)

Large-Scale Interlaboratory Study to Develop, Analytically Validate and Apply Highly Multiplexed, Quantitative Peptide Assays to Measure Cancer-Relevant Proteins in Plasma. Abbatiello SE, Schilling B, Mani DR, Zimmerman LJ, Hall SC, MacLean B, Albertolle M, Allen S, Burgess M, Cusack MP, Gosh M, Hedrick V, Held JM, Inerowicz HD, Jackson A, Keshishian H, Kinsinger CR, Lyssand J, Makowski L, Mesri M, Rodriguez H, Rudnick P, Sadowski P, Sedransk N, Shaddox K, Skates SJ, Kuhn E, Smith D, Whiteaker JR, Whitwell C, Zhang S, Borchers CH, Fisher SJ, Gibson BW, Liebler DC, MacCoss MJ, Neubert TA, Paulovich AG, Regnier FE, Tempst P, Carr SA. Mol Cell Proteomics. 2015 Sep;14(9):2357-74. doi: 10.1074/mcp.M114.047050. Epub 2015 Feb 18. PMID: 25693799


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Quantiva TSQ
Internal Standard
heavy stable isotope peptide (delta 8-10)
Peptide Standard Purity
>95%
Peptide Standard Label Type
15N
LC
Easy NanoLC1000
Column Packing
Reprosil C18, 1.9um, 200A
Column Dimensions
0.075 x 150 mm
Flow Rate
200 nL/min

Assay Multiplexing Expand assay panel

Broad Institute-Plasma-based Candidate Cancer Biomarkers

CPTAC-1372:
ALDOC.
ALQASALNAWR
CPTAC-1373:
ALDOC.
AEVNGLAAQGK
CPTAC-1374:
ALDOC.
ELSDIALR
CPTAC-1375:
ALDOC.
TPSALAILENANVLAR
CPTAC-1376:
ALDOC.
QVLFSADDR
CPTAC-1377:
ANXA1.
ALYEAGER
CPTAC-1378:
ANXA1.
GTDVNVFNTILTTR
CPTAC-1379:
ANXA1.
AAYLQETGKPLDETLK
CPTAC-1380:
ANXA1.
TPAQFDADELR
CPTAC-1381:
ANXA4.
VLVSLSAGGR
CPTAC-1382:
ANXA4.
GLGTDEDAIISVLAYR
CPTAC-1383:
ANXA4.
DEGNYLDDALVR
CPTAC-1384:
ANXA4.
GAGTDEGC[+57.0]LIEILASR
CPTAC-1385:
ANXA7.
LYQAGEGR
CPTAC-1386:
ANXA7.
SEIDLVQIK
CPTAC-1387:
ANXA7.
GAGTDDSTLVR
CPTAC-1388:
ANXA7.
EFSGYVESGLK
CPTAC-1389:
ANXA7.
GFGTDEQAIVDVVANR
CPTAC-1390:
CALR.
QIDNPDYK
CPTAC-1391:
CALR.
FYALSASFEPFSNK
CPTAC-1392:
CALR.
EQFLDGDGWTSR
CPTAC-1393:
CLIC1.
LHIVQVVC[+57.0]K
CPTAC-1394:
CLIC1.
YLSNAYAR
CPTAC-1395:
CLIC1.
IEEFLEAVLC[+57.0]PPR
CPTAC-1396:
CLIC1.
GVTFNVTTVDTK
CPTAC-1397:
CLIC1.
GFTIPEAFR
CPTAC-1398:
EZR.
IQVWHAEHR
CPTAC-1399:
EZR.
EDEVEEWQHR
CPTAC-1400:
EZR.
SGYLSSER
CPTAC-1401:
EZR.
IALLEEAR
CPTAC-1402:
EZR.
SQEQLAAELAEYTAK
CPTAC-1403:
FSCN1.
FLIVAHDDGR
CPTAC-1404:
FSCN1.
YLAPSGPSGTLK
CPTAC-1405:
FSCN1.
VTGTLDANR
CPTAC-1406:
FSCN1.
LSC[+57.0]FAQTVSPAEK
CPTAC-1407:
FTL.
LGGPEAGLGEYLFER
CPTAC-1408:
FTL.
KPAEDEWGK
CPTAC-1409:
FEN1.
SIEEIVR
CPTAC-1410:
FEN1.
QLQQAQAAGAEQEVEK
CPTAC-1411:
FEN1.
LIADVAPSAIR
CPTAC-1412:
GSTP1.
FQDGDLTLYQSNTILR
CPTAC-1413:
GSTP1.
EEVVTVETWQEGSLK
CPTAC-1414:
GSTP1.
PPYTVVYFPVR
CPTAC-1415:
GSTP1.
ASC[+57.0]LYGQLPK
CPTAC-1416:
GLO1.
FSLYFLAYEDK
CPTAC-1417:
GLO1.
SLDFYTR
CPTAC-1418:
GLO1.
IAWALSR
CPTAC-1419:
GLO1.
GFGHIGIAVPDVYSAC[+57.0]K
CPTAC-1420:
GLO1.
FEELGVK
CPTAC-1421:
GRB2.
FNSLNELVDYHR
CPTAC-1422:
GRB2.
FGNDVQHFK
CPTAC-1423:
GRB2.
NYVTPVNR
CPTAC-1424:
GRB2.
ATADDELSFK
CPTAC-1425:
GRB2.
ESESAPGDFSLSVK
CPTAC-1426:
HSPB1.
LFDQAFGLPR
CPTAC-1427:
HSPB1.
AQLGGPEAAK
CPTAC-1428:
HSPB1.
VSLDVNHFAPDELTVK
CPTAC-1429:
HSPB1.
DGVVEITGK
CPTAC-1430:
HSPB1.
QLSSGVSEIR
CPTAC-1431:
IL18.
EDELGDR
CPTAC-1432:
IL18.
SDIIFFQR
CPTAC-1433:
IL18.
TIFIISMYK
CPTAC-1434:
IL18.
ISTLSC[+57.0]ENK
CPTAC-1435:
IL18.
GMAVTISVK
CPTAC-1436:
LGALS1.
DGGAWGTEQR
CPTAC-1437:
LGALS1.
DSNNLC[+57.0]LHFNPR
CPTAC-1438:
LGALS1.
FNAHGDANTIVC[+57.0]NSK
CPTAC-1439:
LGALS1.
SFVLNLGK
CPTAC-1440:
LGALS1.
LPDGYEFK
CPTAC-1441:
PDLIM1.
C[+57.0]GTGIVGVFVK
CPTAC-1442:
PDLIM1.
VAASIGNAQK
CPTAC-1443:
PDLIM1.
VWSPLVTEEGK
CPTAC-1444:
PDLIM1.
DFEQPLAISR
CPTAC-1445:
PRDX2.
GLFIIDGK
CPTAC-1446:
PRDX2.
TDEGIAYR
CPTAC-1447:
PRDX2.
ATAVVDGAFK
CPTAC-1448:
PRDX4.
DYGVYLEDSGHTLR
CPTAC-1449:
PRDX4.
LVQAFQYTDK
CPTAC-1450:
PRDX4.
IPLLSDLTHQISK
CPTAC-1451:
PRDX4.
QITLNDLPVGR
CPTAC-1452:
S100A1.
ELLQTELSGFLDAQK
CPTAC-1453:
S100A2.
ELPSFVGEK
CPTAC-1454:
S100B.
AMVALIDVFHQYSGR
CPTAC-1455:
S100B.
ELINNELSHFLEEIK
CPTAC-1456:
RAD23B.
EQVIAALR
CPTAC-1457:
RAD23B.
IDIDPEETVK
CPTAC-1458:
RAD23B.
ILNDDTALK
CPTAC-1459:
SNCG.
EGVVGAVEK
CPTAC-1460:
SNCG.
ENVVQSVTSVAEK
CPTAC-1461:
SNCG.
TVEEAENIAVTSGVVR
CPTAC-1462:
TPM1.
LVIIESDLER
CPTAC-1463:
TPM1.
SIDDLEDELYAQK
CPTAC-1464:
TPM1.
QLEDELVSLQK
CPTAC-1465:
UBE2C.
YLQETYSK
CPTAC-1466:
UBE2C.
LSLEFPSGYPYNAPTVK
CPTAC-1467:
UBE2C.
DPAATSVAAAR
CPTAC-1468:
UBE2C.
GISAFPESDNLFK
CPTAC-1469:
UBE2I.
DDYPSSPPK
CPTAC-1470:
UBE2I.
DWRPAITIK
CPTAC-1471:
UBE2I.
DHPFGFVAVPTK

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (1+) 34.5 15 10.4 41.4 19.8 10.2 53.9 24.8 14.6 15 15 15
y10 (2+) 60.9 8.4 7.9 63.4 10.5 12.3 87.9 13.4 14.6 15 15 15
y8 (1+) 5.6 2.5 3 8.2 5.9 4.7 9.9 6.4 5.6 15 15 15
sum 5 2.6 2.8 7.8 5.8 4.4 9.3 6.4 5.2 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-1444 Collapse assay details

Data source: Panorama

Official Gene Symbol
PDLIM1
Peptide Sequence
DFEQPLAISR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
23
Peptide End
32
CPTAC ID
CPTAC-1444
Peptide Molecular Mass
1,174.5982
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
depleted digested plasma
Submitting Laboratory
Broad Institute
Submitting Lab PI
Steven A. Carr

Publication

View Details (opens in a new window)

Large-Scale Interlaboratory Study to Develop, Analytically Validate and Apply Highly Multiplexed, Quantitative Peptide Assays to Measure Cancer-Relevant Proteins in Plasma. Abbatiello SE, Schilling B, Mani DR, Zimmerman LJ, Hall SC, MacLean B, Albertolle M, Allen S, Burgess M, Cusack MP, Gosh M, Hedrick V, Held JM, Inerowicz HD, Jackson A, Keshishian H, Kinsinger CR, Lyssand J, Makowski L, Mesri M, Rodriguez H, Rudnick P, Sadowski P, Sedransk N, Shaddox K, Skates SJ, Kuhn E, Smith D, Whiteaker JR, Whitwell C, Zhang S, Borchers CH, Fisher SJ, Gibson BW, Liebler DC, MacCoss MJ, Neubert TA, Paulovich AG, Regnier FE, Tempst P, Carr SA. Mol Cell Proteomics. 2015 Sep;14(9):2357-74. doi: 10.1074/mcp.M114.047050. Epub 2015 Feb 18. PMID: 25693799


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Quantiva TSQ
Internal Standard
heavy stable isotope peptide (delta 8-10)
Peptide Standard Purity
>95%
Peptide Standard Label Type
15N
LC
Easy NanoLC1000
Column Packing
Reprosil C18, 1.9um, 200A
Column Dimensions
0.075 x 150 mm
Flow Rate
200 nL/min

Assay Multiplexing Expand assay panel

Broad Institute-Plasma-based Candidate Cancer Biomarkers

CPTAC-1372:
ALDOC.
ALQASALNAWR
CPTAC-1373:
ALDOC.
AEVNGLAAQGK
CPTAC-1374:
ALDOC.
ELSDIALR
CPTAC-1375:
ALDOC.
TPSALAILENANVLAR
CPTAC-1376:
ALDOC.
QVLFSADDR
CPTAC-1377:
ANXA1.
ALYEAGER
CPTAC-1378:
ANXA1.
GTDVNVFNTILTTR
CPTAC-1379:
ANXA1.
AAYLQETGKPLDETLK
CPTAC-1380:
ANXA1.
TPAQFDADELR
CPTAC-1381:
ANXA4.
VLVSLSAGGR
CPTAC-1382:
ANXA4.
GLGTDEDAIISVLAYR
CPTAC-1383:
ANXA4.
DEGNYLDDALVR
CPTAC-1384:
ANXA4.
GAGTDEGC[+57.0]LIEILASR
CPTAC-1385:
ANXA7.
LYQAGEGR
CPTAC-1386:
ANXA7.
SEIDLVQIK
CPTAC-1387:
ANXA7.
GAGTDDSTLVR
CPTAC-1388:
ANXA7.
EFSGYVESGLK
CPTAC-1389:
ANXA7.
GFGTDEQAIVDVVANR
CPTAC-1390:
CALR.
QIDNPDYK
CPTAC-1391:
CALR.
FYALSASFEPFSNK
CPTAC-1392:
CALR.
EQFLDGDGWTSR
CPTAC-1393:
CLIC1.
LHIVQVVC[+57.0]K
CPTAC-1394:
CLIC1.
YLSNAYAR
CPTAC-1395:
CLIC1.
IEEFLEAVLC[+57.0]PPR
CPTAC-1396:
CLIC1.
GVTFNVTTVDTK
CPTAC-1397:
CLIC1.
GFTIPEAFR
CPTAC-1398:
EZR.
IQVWHAEHR
CPTAC-1399:
EZR.
EDEVEEWQHR
CPTAC-1400:
EZR.
SGYLSSER
CPTAC-1401:
EZR.
IALLEEAR
CPTAC-1402:
EZR.
SQEQLAAELAEYTAK
CPTAC-1403:
FSCN1.
FLIVAHDDGR
CPTAC-1404:
FSCN1.
YLAPSGPSGTLK
CPTAC-1405:
FSCN1.
VTGTLDANR
CPTAC-1406:
FSCN1.
LSC[+57.0]FAQTVSPAEK
CPTAC-1407:
FTL.
LGGPEAGLGEYLFER
CPTAC-1408:
FTL.
KPAEDEWGK
CPTAC-1409:
FEN1.
SIEEIVR
CPTAC-1410:
FEN1.
QLQQAQAAGAEQEVEK
CPTAC-1411:
FEN1.
LIADVAPSAIR
CPTAC-1412:
GSTP1.
FQDGDLTLYQSNTILR
CPTAC-1413:
GSTP1.
EEVVTVETWQEGSLK
CPTAC-1414:
GSTP1.
PPYTVVYFPVR
CPTAC-1415:
GSTP1.
ASC[+57.0]LYGQLPK
CPTAC-1416:
GLO1.
FSLYFLAYEDK
CPTAC-1417:
GLO1.
SLDFYTR
CPTAC-1418:
GLO1.
IAWALSR
CPTAC-1419:
GLO1.
GFGHIGIAVPDVYSAC[+57.0]K
CPTAC-1420:
GLO1.
FEELGVK
CPTAC-1421:
GRB2.
FNSLNELVDYHR
CPTAC-1422:
GRB2.
FGNDVQHFK
CPTAC-1423:
GRB2.
NYVTPVNR
CPTAC-1424:
GRB2.
ATADDELSFK
CPTAC-1425:
GRB2.
ESESAPGDFSLSVK
CPTAC-1426:
HSPB1.
LFDQAFGLPR
CPTAC-1427:
HSPB1.
AQLGGPEAAK
CPTAC-1428:
HSPB1.
VSLDVNHFAPDELTVK
CPTAC-1429:
HSPB1.
DGVVEITGK
CPTAC-1430:
HSPB1.
QLSSGVSEIR
CPTAC-1431:
IL18.
EDELGDR
CPTAC-1432:
IL18.
SDIIFFQR
CPTAC-1433:
IL18.
TIFIISMYK
CPTAC-1434:
IL18.
ISTLSC[+57.0]ENK
CPTAC-1435:
IL18.
GMAVTISVK
CPTAC-1436:
LGALS1.
DGGAWGTEQR
CPTAC-1437:
LGALS1.
DSNNLC[+57.0]LHFNPR
CPTAC-1438:
LGALS1.
FNAHGDANTIVC[+57.0]NSK
CPTAC-1439:
LGALS1.
SFVLNLGK
CPTAC-1440:
LGALS1.
LPDGYEFK
CPTAC-1441:
PDLIM1.
C[+57.0]GTGIVGVFVK
CPTAC-1442:
PDLIM1.
VAASIGNAQK
CPTAC-1443:
PDLIM1.
VWSPLVTEEGK
CPTAC-1444:
PDLIM1.
DFEQPLAISR
CPTAC-1445:
PRDX2.
GLFIIDGK
CPTAC-1446:
PRDX2.
TDEGIAYR
CPTAC-1447:
PRDX2.
ATAVVDGAFK
CPTAC-1448:
PRDX4.
DYGVYLEDSGHTLR
CPTAC-1449:
PRDX4.
LVQAFQYTDK
CPTAC-1450:
PRDX4.
IPLLSDLTHQISK
CPTAC-1451:
PRDX4.
QITLNDLPVGR
CPTAC-1452:
S100A1.
ELLQTELSGFLDAQK
CPTAC-1453:
S100A2.
ELPSFVGEK
CPTAC-1454:
S100B.
AMVALIDVFHQYSGR
CPTAC-1455:
S100B.
ELINNELSHFLEEIK
CPTAC-1456:
RAD23B.
EQVIAALR
CPTAC-1457:
RAD23B.
IDIDPEETVK
CPTAC-1458:
RAD23B.
ILNDDTALK
CPTAC-1459:
SNCG.
EGVVGAVEK
CPTAC-1460:
SNCG.
ENVVQSVTSVAEK
CPTAC-1461:
SNCG.
TVEEAENIAVTSGVVR
CPTAC-1462:
TPM1.
LVIIESDLER
CPTAC-1463:
TPM1.
SIDDLEDELYAQK
CPTAC-1464:
TPM1.
QLEDELVSLQK
CPTAC-1465:
UBE2C.
YLQETYSK
CPTAC-1466:
UBE2C.
LSLEFPSGYPYNAPTVK
CPTAC-1467:
UBE2C.
DPAATSVAAAR
CPTAC-1468:
UBE2C.
GISAFPESDNLFK
CPTAC-1469:
UBE2I.
DDYPSSPPK
CPTAC-1470:
UBE2I.
DWRPAITIK
CPTAC-1471:
UBE2I.
DHPFGFVAVPTK

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 4.8 2.5 3.2 7.2 6.6 3.8 8.7 7.1 5 15 15 15
y4 (1+) 4.8 1.9 1.7 9.2 6.1 3.5 10.4 6.4 3.9 15 15 15
y6 (1+) 2.7 1.7 2.1 6.4 6.1 3.5 6.9 6.3 4.1 15 15 15
sum 2.1 1.5 1.9 6.4 6.1 3.4 6.7 6.3 3.9 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-1442 Collapse assay details

Data source: Panorama

Official Gene Symbol
PDLIM1
Peptide Sequence
VAASIGNAQK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
247
Peptide End
256
CPTAC ID
CPTAC-1442
Peptide Molecular Mass
957.5243
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
depleted digested plasma
Submitting Laboratory
Broad Institute
Submitting Lab PI
Steven A. Carr

Publication

View Details (opens in a new window)

Large-Scale Interlaboratory Study to Develop, Analytically Validate and Apply Highly Multiplexed, Quantitative Peptide Assays to Measure Cancer-Relevant Proteins in Plasma. Abbatiello SE, Schilling B, Mani DR, Zimmerman LJ, Hall SC, MacLean B, Albertolle M, Allen S, Burgess M, Cusack MP, Gosh M, Hedrick V, Held JM, Inerowicz HD, Jackson A, Keshishian H, Kinsinger CR, Lyssand J, Makowski L, Mesri M, Rodriguez H, Rudnick P, Sadowski P, Sedransk N, Shaddox K, Skates SJ, Kuhn E, Smith D, Whiteaker JR, Whitwell C, Zhang S, Borchers CH, Fisher SJ, Gibson BW, Liebler DC, MacCoss MJ, Neubert TA, Paulovich AG, Regnier FE, Tempst P, Carr SA. Mol Cell Proteomics. 2015 Sep;14(9):2357-74. doi: 10.1074/mcp.M114.047050. Epub 2015 Feb 18. PMID: 25693799


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Quantiva TSQ
Internal Standard
heavy stable isotope peptide (delta 8-10)
Peptide Standard Purity
>95%
Peptide Standard Label Type
15N
LC
Easy NanoLC1000
Column Packing
Reprosil C18, 1.9um, 200A
Column Dimensions
0.075 x 150 mm
Flow Rate
200 nL/min

Assay Multiplexing Expand assay panel

Broad Institute-Plasma-based Candidate Cancer Biomarkers

CPTAC-1372:
ALDOC.
ALQASALNAWR
CPTAC-1373:
ALDOC.
AEVNGLAAQGK
CPTAC-1374:
ALDOC.
ELSDIALR
CPTAC-1375:
ALDOC.
TPSALAILENANVLAR
CPTAC-1376:
ALDOC.
QVLFSADDR
CPTAC-1377:
ANXA1.
ALYEAGER
CPTAC-1378:
ANXA1.
GTDVNVFNTILTTR
CPTAC-1379:
ANXA1.
AAYLQETGKPLDETLK
CPTAC-1380:
ANXA1.
TPAQFDADELR
CPTAC-1381:
ANXA4.
VLVSLSAGGR
CPTAC-1382:
ANXA4.
GLGTDEDAIISVLAYR
CPTAC-1383:
ANXA4.
DEGNYLDDALVR
CPTAC-1384:
ANXA4.
GAGTDEGC[+57.0]LIEILASR
CPTAC-1385:
ANXA7.
LYQAGEGR
CPTAC-1386:
ANXA7.
SEIDLVQIK
CPTAC-1387:
ANXA7.
GAGTDDSTLVR
CPTAC-1388:
ANXA7.
EFSGYVESGLK
CPTAC-1389:
ANXA7.
GFGTDEQAIVDVVANR
CPTAC-1390:
CALR.
QIDNPDYK
CPTAC-1391:
CALR.
FYALSASFEPFSNK
CPTAC-1392:
CALR.
EQFLDGDGWTSR
CPTAC-1393:
CLIC1.
LHIVQVVC[+57.0]K
CPTAC-1394:
CLIC1.
YLSNAYAR
CPTAC-1395:
CLIC1.
IEEFLEAVLC[+57.0]PPR
CPTAC-1396:
CLIC1.
GVTFNVTTVDTK
CPTAC-1397:
CLIC1.
GFTIPEAFR
CPTAC-1398:
EZR.
IQVWHAEHR
CPTAC-1399:
EZR.
EDEVEEWQHR
CPTAC-1400:
EZR.
SGYLSSER
CPTAC-1401:
EZR.
IALLEEAR
CPTAC-1402:
EZR.
SQEQLAAELAEYTAK
CPTAC-1403:
FSCN1.
FLIVAHDDGR
CPTAC-1404:
FSCN1.
YLAPSGPSGTLK
CPTAC-1405:
FSCN1.
VTGTLDANR
CPTAC-1406:
FSCN1.
LSC[+57.0]FAQTVSPAEK
CPTAC-1407:
FTL.
LGGPEAGLGEYLFER
CPTAC-1408:
FTL.
KPAEDEWGK
CPTAC-1409:
FEN1.
SIEEIVR
CPTAC-1410:
FEN1.
QLQQAQAAGAEQEVEK
CPTAC-1411:
FEN1.
LIADVAPSAIR
CPTAC-1412:
GSTP1.
FQDGDLTLYQSNTILR
CPTAC-1413:
GSTP1.
EEVVTVETWQEGSLK
CPTAC-1414:
GSTP1.
PPYTVVYFPVR
CPTAC-1415:
GSTP1.
ASC[+57.0]LYGQLPK
CPTAC-1416:
GLO1.
FSLYFLAYEDK
CPTAC-1417:
GLO1.
SLDFYTR
CPTAC-1418:
GLO1.
IAWALSR
CPTAC-1419:
GLO1.
GFGHIGIAVPDVYSAC[+57.0]K
CPTAC-1420:
GLO1.
FEELGVK
CPTAC-1421:
GRB2.
FNSLNELVDYHR
CPTAC-1422:
GRB2.
FGNDVQHFK
CPTAC-1423:
GRB2.
NYVTPVNR
CPTAC-1424:
GRB2.
ATADDELSFK
CPTAC-1425:
GRB2.
ESESAPGDFSLSVK
CPTAC-1426:
HSPB1.
LFDQAFGLPR
CPTAC-1427:
HSPB1.
AQLGGPEAAK
CPTAC-1428:
HSPB1.
VSLDVNHFAPDELTVK
CPTAC-1429:
HSPB1.
DGVVEITGK
CPTAC-1430:
HSPB1.
QLSSGVSEIR
CPTAC-1431:
IL18.
EDELGDR
CPTAC-1432:
IL18.
SDIIFFQR
CPTAC-1433:
IL18.
TIFIISMYK
CPTAC-1434:
IL18.
ISTLSC[+57.0]ENK
CPTAC-1435:
IL18.
GMAVTISVK
CPTAC-1436:
LGALS1.
DGGAWGTEQR
CPTAC-1437:
LGALS1.
DSNNLC[+57.0]LHFNPR
CPTAC-1438:
LGALS1.
FNAHGDANTIVC[+57.0]NSK
CPTAC-1439:
LGALS1.
SFVLNLGK
CPTAC-1440:
LGALS1.
LPDGYEFK
CPTAC-1441:
PDLIM1.
C[+57.0]GTGIVGVFVK
CPTAC-1442:
PDLIM1.
VAASIGNAQK
CPTAC-1443:
PDLIM1.
VWSPLVTEEGK
CPTAC-1444:
PDLIM1.
DFEQPLAISR
CPTAC-1445:
PRDX2.
GLFIIDGK
CPTAC-1446:
PRDX2.
TDEGIAYR
CPTAC-1447:
PRDX2.
ATAVVDGAFK
CPTAC-1448:
PRDX4.
DYGVYLEDSGHTLR
CPTAC-1449:
PRDX4.
LVQAFQYTDK
CPTAC-1450:
PRDX4.
IPLLSDLTHQISK
CPTAC-1451:
PRDX4.
QITLNDLPVGR
CPTAC-1452:
S100A1.
ELLQTELSGFLDAQK
CPTAC-1453:
S100A2.
ELPSFVGEK
CPTAC-1454:
S100B.
AMVALIDVFHQYSGR
CPTAC-1455:
S100B.
ELINNELSHFLEEIK
CPTAC-1456:
RAD23B.
EQVIAALR
CPTAC-1457:
RAD23B.
IDIDPEETVK
CPTAC-1458:
RAD23B.
ILNDDTALK
CPTAC-1459:
SNCG.
EGVVGAVEK
CPTAC-1460:
SNCG.
ENVVQSVTSVAEK
CPTAC-1461:
SNCG.
TVEEAENIAVTSGVVR
CPTAC-1462:
TPM1.
LVIIESDLER
CPTAC-1463:
TPM1.
SIDDLEDELYAQK
CPTAC-1464:
TPM1.
QLEDELVSLQK
CPTAC-1465:
UBE2C.
YLQETYSK
CPTAC-1466:
UBE2C.
LSLEFPSGYPYNAPTVK
CPTAC-1467:
UBE2C.
DPAATSVAAAR
CPTAC-1468:
UBE2C.
GISAFPESDNLFK
CPTAC-1469:
UBE2I.
DDYPSSPPK
CPTAC-1470:
UBE2I.
DWRPAITIK
CPTAC-1471:
UBE2I.
DHPFGFVAVPTK

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (1+) 2.5 2 2.5 3.4 7 3.9 4.2 7.3 4.6 15 15 15
y8 (1+) 4.3 2.8 2.7 5 7.3 4 6.6 7.8 4.8 15 15 15
y7 (1+) 6.6 2.8 2.4 7 7.5 3.3 9.6 8 4.1 15 15 15
sum 2.2 2.2 2.3 3.7 7.1 3.7 4.3 7.4 4.4 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-1443 Collapse assay details

Data source: Panorama

Official Gene Symbol
PDLIM1
Peptide Sequence
VWSPLVTEEGK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
88
Peptide End
98
CPTAC ID
CPTAC-1443
Peptide Molecular Mass
1,243.6449
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
depleted digested plasma
Submitting Laboratory
Broad Institute
Submitting Lab PI
Steven A. Carr

Publication

View Details (opens in a new window)

Large-Scale Interlaboratory Study to Develop, Analytically Validate and Apply Highly Multiplexed, Quantitative Peptide Assays to Measure Cancer-Relevant Proteins in Plasma. Abbatiello SE, Schilling B, Mani DR, Zimmerman LJ, Hall SC, MacLean B, Albertolle M, Allen S, Burgess M, Cusack MP, Gosh M, Hedrick V, Held JM, Inerowicz HD, Jackson A, Keshishian H, Kinsinger CR, Lyssand J, Makowski L, Mesri M, Rodriguez H, Rudnick P, Sadowski P, Sedransk N, Shaddox K, Skates SJ, Kuhn E, Smith D, Whiteaker JR, Whitwell C, Zhang S, Borchers CH, Fisher SJ, Gibson BW, Liebler DC, MacCoss MJ, Neubert TA, Paulovich AG, Regnier FE, Tempst P, Carr SA. Mol Cell Proteomics. 2015 Sep;14(9):2357-74. doi: 10.1074/mcp.M114.047050. Epub 2015 Feb 18. PMID: 25693799


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Quantiva TSQ
Internal Standard
heavy stable isotope peptide (delta 8-10)
Peptide Standard Purity
>95%
Peptide Standard Label Type
15N
LC
Easy NanoLC1000
Column Packing
Reprosil C18, 1.9um, 200A
Column Dimensions
0.075 x 150 mm
Flow Rate
200 nL/min

Assay Multiplexing Expand assay panel

Broad Institute-Plasma-based Candidate Cancer Biomarkers

CPTAC-1372:
ALDOC.
ALQASALNAWR
CPTAC-1373:
ALDOC.
AEVNGLAAQGK
CPTAC-1374:
ALDOC.
ELSDIALR
CPTAC-1375:
ALDOC.
TPSALAILENANVLAR
CPTAC-1376:
ALDOC.
QVLFSADDR
CPTAC-1377:
ANXA1.
ALYEAGER
CPTAC-1378:
ANXA1.
GTDVNVFNTILTTR
CPTAC-1379:
ANXA1.
AAYLQETGKPLDETLK
CPTAC-1380:
ANXA1.
TPAQFDADELR
CPTAC-1381:
ANXA4.
VLVSLSAGGR
CPTAC-1382:
ANXA4.
GLGTDEDAIISVLAYR
CPTAC-1383:
ANXA4.
DEGNYLDDALVR
CPTAC-1384:
ANXA4.
GAGTDEGC[+57.0]LIEILASR
CPTAC-1385:
ANXA7.
LYQAGEGR
CPTAC-1386:
ANXA7.
SEIDLVQIK
CPTAC-1387:
ANXA7.
GAGTDDSTLVR
CPTAC-1388:
ANXA7.
EFSGYVESGLK
CPTAC-1389:
ANXA7.
GFGTDEQAIVDVVANR
CPTAC-1390:
CALR.
QIDNPDYK
CPTAC-1391:
CALR.
FYALSASFEPFSNK
CPTAC-1392:
CALR.
EQFLDGDGWTSR
CPTAC-1393:
CLIC1.
LHIVQVVC[+57.0]K
CPTAC-1394:
CLIC1.
YLSNAYAR
CPTAC-1395:
CLIC1.
IEEFLEAVLC[+57.0]PPR
CPTAC-1396:
CLIC1.
GVTFNVTTVDTK
CPTAC-1397:
CLIC1.
GFTIPEAFR
CPTAC-1398:
EZR.
IQVWHAEHR
CPTAC-1399:
EZR.
EDEVEEWQHR
CPTAC-1400:
EZR.
SGYLSSER
CPTAC-1401:
EZR.
IALLEEAR
CPTAC-1402:
EZR.
SQEQLAAELAEYTAK
CPTAC-1403:
FSCN1.
FLIVAHDDGR
CPTAC-1404:
FSCN1.
YLAPSGPSGTLK
CPTAC-1405:
FSCN1.
VTGTLDANR
CPTAC-1406:
FSCN1.
LSC[+57.0]FAQTVSPAEK
CPTAC-1407:
FTL.
LGGPEAGLGEYLFER
CPTAC-1408:
FTL.
KPAEDEWGK
CPTAC-1409:
FEN1.
SIEEIVR
CPTAC-1410:
FEN1.
QLQQAQAAGAEQEVEK
CPTAC-1411:
FEN1.
LIADVAPSAIR
CPTAC-1412:
GSTP1.
FQDGDLTLYQSNTILR
CPTAC-1413:
GSTP1.
EEVVTVETWQEGSLK
CPTAC-1414:
GSTP1.
PPYTVVYFPVR
CPTAC-1415:
GSTP1.
ASC[+57.0]LYGQLPK
CPTAC-1416:
GLO1.
FSLYFLAYEDK
CPTAC-1417:
GLO1.
SLDFYTR
CPTAC-1418:
GLO1.
IAWALSR
CPTAC-1419:
GLO1.
GFGHIGIAVPDVYSAC[+57.0]K
CPTAC-1420:
GLO1.
FEELGVK
CPTAC-1421:
GRB2.
FNSLNELVDYHR
CPTAC-1422:
GRB2.
FGNDVQHFK
CPTAC-1423:
GRB2.
NYVTPVNR
CPTAC-1424:
GRB2.
ATADDELSFK
CPTAC-1425:
GRB2.
ESESAPGDFSLSVK
CPTAC-1426:
HSPB1.
LFDQAFGLPR
CPTAC-1427:
HSPB1.
AQLGGPEAAK
CPTAC-1428:
HSPB1.
VSLDVNHFAPDELTVK
CPTAC-1429:
HSPB1.
DGVVEITGK
CPTAC-1430:
HSPB1.
QLSSGVSEIR
CPTAC-1431:
IL18.
EDELGDR
CPTAC-1432:
IL18.
SDIIFFQR
CPTAC-1433:
IL18.
TIFIISMYK
CPTAC-1434:
IL18.
ISTLSC[+57.0]ENK
CPTAC-1435:
IL18.
GMAVTISVK
CPTAC-1436:
LGALS1.
DGGAWGTEQR
CPTAC-1437:
LGALS1.
DSNNLC[+57.0]LHFNPR
CPTAC-1438:
LGALS1.
FNAHGDANTIVC[+57.0]NSK
CPTAC-1439:
LGALS1.
SFVLNLGK
CPTAC-1440:
LGALS1.
LPDGYEFK
CPTAC-1441:
PDLIM1.
C[+57.0]GTGIVGVFVK
CPTAC-1442:
PDLIM1.
VAASIGNAQK
CPTAC-1443:
PDLIM1.
VWSPLVTEEGK
CPTAC-1444:
PDLIM1.
DFEQPLAISR
CPTAC-1445:
PRDX2.
GLFIIDGK
CPTAC-1446:
PRDX2.
TDEGIAYR
CPTAC-1447:
PRDX2.
ATAVVDGAFK
CPTAC-1448:
PRDX4.
DYGVYLEDSGHTLR
CPTAC-1449:
PRDX4.
LVQAFQYTDK
CPTAC-1450:
PRDX4.
IPLLSDLTHQISK
CPTAC-1451:
PRDX4.
QITLNDLPVGR
CPTAC-1452:
S100A1.
ELLQTELSGFLDAQK
CPTAC-1453:
S100A2.
ELPSFVGEK
CPTAC-1454:
S100B.
AMVALIDVFHQYSGR
CPTAC-1455:
S100B.
ELINNELSHFLEEIK
CPTAC-1456:
RAD23B.
EQVIAALR
CPTAC-1457:
RAD23B.
IDIDPEETVK
CPTAC-1458:
RAD23B.
ILNDDTALK
CPTAC-1459:
SNCG.
EGVVGAVEK
CPTAC-1460:
SNCG.
ENVVQSVTSVAEK
CPTAC-1461:
SNCG.
TVEEAENIAVTSGVVR
CPTAC-1462:
TPM1.
LVIIESDLER
CPTAC-1463:
TPM1.
SIDDLEDELYAQK
CPTAC-1464:
TPM1.
QLEDELVSLQK
CPTAC-1465:
UBE2C.
YLQETYSK
CPTAC-1466:
UBE2C.
LSLEFPSGYPYNAPTVK
CPTAC-1467:
UBE2C.
DPAATSVAAAR
CPTAC-1468:
UBE2C.
GISAFPESDNLFK
CPTAC-1469:
UBE2I.
DDYPSSPPK
CPTAC-1470:
UBE2I.
DWRPAITIK
CPTAC-1471:
UBE2I.
DHPFGFVAVPTK

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (1+) 4.4 2.2 2.5 6.9 5.9 5.1 8.2 6.3 5.7 15 15 15
y8 (1+) 2.9 1.4 2.1 6.1 6.8 4.3 6.8 6.9 4.8 15 15 15
y9 (1+) 3 1.8 1.6 5.2 6.3 4.1 6 6.6 4.4 15 15 15
sum 1.8 1.2 1.8 5.2 6.3 4.3 5.5 6.4 4.7 15 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-3992 Collapse assay details

Data source: Panorama

Official Gene Symbol
Pdlim1
Peptide Sequence
DFEQPLAISR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
23
Peptide End
32
CPTAC ID
non-CPTAC-3992
Peptide Molecular Mass
1,174.5982
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Assay Multiplexing Expand assay panel

Broad Institute-Plasma-based Candidate Cancer Biomarkers

CPTAC-1372:
ALDOC.
ALQASALNAWR
CPTAC-1373:
ALDOC.
AEVNGLAAQGK
CPTAC-1374:
ALDOC.
ELSDIALR
CPTAC-1375:
ALDOC.
TPSALAILENANVLAR
CPTAC-1376:
ALDOC.
QVLFSADDR
CPTAC-1377:
ANXA1.
ALYEAGER
CPTAC-1378:
ANXA1.
GTDVNVFNTILTTR
CPTAC-1379:
ANXA1.
AAYLQETGKPLDETLK
CPTAC-1380:
ANXA1.
TPAQFDADELR
CPTAC-1381:
ANXA4.
VLVSLSAGGR
CPTAC-1382:
ANXA4.
GLGTDEDAIISVLAYR
CPTAC-1383:
ANXA4.
DEGNYLDDALVR
CPTAC-1384:
ANXA4.
GAGTDEGC[+57.0]LIEILASR
CPTAC-1385:
ANXA7.
LYQAGEGR
CPTAC-1386:
ANXA7.
SEIDLVQIK
CPTAC-1387:
ANXA7.
GAGTDDSTLVR
CPTAC-1388:
ANXA7.
EFSGYVESGLK
CPTAC-1389:
ANXA7.
GFGTDEQAIVDVVANR
CPTAC-1390:
CALR.
QIDNPDYK
CPTAC-1391:
CALR.
FYALSASFEPFSNK
CPTAC-1392:
CALR.
EQFLDGDGWTSR
CPTAC-1393:
CLIC1.
LHIVQVVC[+57.0]K
CPTAC-1394:
CLIC1.
YLSNAYAR
CPTAC-1395:
CLIC1.
IEEFLEAVLC[+57.0]PPR
CPTAC-1396:
CLIC1.
GVTFNVTTVDTK
CPTAC-1397:
CLIC1.
GFTIPEAFR
CPTAC-1398:
EZR.
IQVWHAEHR
CPTAC-1399:
EZR.
EDEVEEWQHR
CPTAC-1400:
EZR.
SGYLSSER
CPTAC-1401:
EZR.
IALLEEAR
CPTAC-1402:
EZR.
SQEQLAAELAEYTAK
CPTAC-1403:
FSCN1.
FLIVAHDDGR
CPTAC-1404:
FSCN1.
YLAPSGPSGTLK
CPTAC-1405:
FSCN1.
VTGTLDANR
CPTAC-1406:
FSCN1.
LSC[+57.0]FAQTVSPAEK
CPTAC-1407:
FTL.
LGGPEAGLGEYLFER
CPTAC-1408:
FTL.
KPAEDEWGK
CPTAC-1409:
FEN1.
SIEEIVR
CPTAC-1410:
FEN1.
QLQQAQAAGAEQEVEK
CPTAC-1411:
FEN1.
LIADVAPSAIR
CPTAC-1412:
GSTP1.
FQDGDLTLYQSNTILR
CPTAC-1413:
GSTP1.
EEVVTVETWQEGSLK
CPTAC-1414:
GSTP1.
PPYTVVYFPVR
CPTAC-1415:
GSTP1.
ASC[+57.0]LYGQLPK
CPTAC-1416:
GLO1.
FSLYFLAYEDK
CPTAC-1417:
GLO1.
SLDFYTR
CPTAC-1418:
GLO1.
IAWALSR
CPTAC-1419:
GLO1.
GFGHIGIAVPDVYSAC[+57.0]K
CPTAC-1420:
GLO1.
FEELGVK
CPTAC-1421:
GRB2.
FNSLNELVDYHR
CPTAC-1422:
GRB2.
FGNDVQHFK
CPTAC-1423:
GRB2.
NYVTPVNR
CPTAC-1424:
GRB2.
ATADDELSFK
CPTAC-1425:
GRB2.
ESESAPGDFSLSVK
CPTAC-1426:
HSPB1.
LFDQAFGLPR
CPTAC-1427:
HSPB1.
AQLGGPEAAK
CPTAC-1428:
HSPB1.
VSLDVNHFAPDELTVK
CPTAC-1429:
HSPB1.
DGVVEITGK
CPTAC-1430:
HSPB1.
QLSSGVSEIR
CPTAC-1431:
IL18.
EDELGDR
CPTAC-1432:
IL18.
SDIIFFQR
CPTAC-1433:
IL18.
TIFIISMYK
CPTAC-1434:
IL18.
ISTLSC[+57.0]ENK
CPTAC-1435:
IL18.
GMAVTISVK
CPTAC-1436:
LGALS1.
DGGAWGTEQR
CPTAC-1437:
LGALS1.
DSNNLC[+57.0]LHFNPR
CPTAC-1438:
LGALS1.
FNAHGDANTIVC[+57.0]NSK
CPTAC-1439:
LGALS1.
SFVLNLGK
CPTAC-1440:
LGALS1.
LPDGYEFK
CPTAC-1441:
PDLIM1.
C[+57.0]GTGIVGVFVK
CPTAC-1442:
PDLIM1.
VAASIGNAQK
CPTAC-1443:
PDLIM1.
VWSPLVTEEGK
CPTAC-1444:
PDLIM1.
DFEQPLAISR
CPTAC-1445:
PRDX2.
GLFIIDGK
CPTAC-1446:
PRDX2.
TDEGIAYR
CPTAC-1447:
PRDX2.
ATAVVDGAFK
CPTAC-1448:
PRDX4.
DYGVYLEDSGHTLR
CPTAC-1449:
PRDX4.
LVQAFQYTDK
CPTAC-1450:
PRDX4.
IPLLSDLTHQISK
CPTAC-1451:
PRDX4.
QITLNDLPVGR
CPTAC-1452:
S100A1.
ELLQTELSGFLDAQK
CPTAC-1453:
S100A2.
ELPSFVGEK
CPTAC-1454:
S100B.
AMVALIDVFHQYSGR
CPTAC-1455:
S100B.
ELINNELSHFLEEIK
CPTAC-1456:
RAD23B.
EQVIAALR
CPTAC-1457:
RAD23B.
IDIDPEETVK
CPTAC-1458:
RAD23B.
ILNDDTALK
CPTAC-1459:
SNCG.
EGVVGAVEK
CPTAC-1460:
SNCG.
ENVVQSVTSVAEK
CPTAC-1461:
SNCG.
TVEEAENIAVTSGVVR
CPTAC-1462:
TPM1.
LVIIESDLER
CPTAC-1463:
TPM1.
SIDDLEDELYAQK
CPTAC-1464:
TPM1.
QLEDELVSLQK
CPTAC-1465:
UBE2C.
YLQETYSK
CPTAC-1466:
UBE2C.
LSLEFPSGYPYNAPTVK
CPTAC-1467:
UBE2C.
DPAATSVAAAR
CPTAC-1468:
UBE2C.
GISAFPESDNLFK
CPTAC-1469:
UBE2I.
DDYPSSPPK
CPTAC-1470:
UBE2I.
DWRPAITIK
CPTAC-1471:
UBE2I.
DHPFGFVAVPTK

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b4 (1+) 6.5 3.1 2.2 7.5 4.8 6.5 9.9 5.7 6.9 15 15 15
b2 (1+) 9.6 3.6 2.9 10.6 4.1 5 14.3 5.5 5.8 15 15 15
y2 (1+) 5.9 2.5 3.8 8.4 5.2 4.4 10.3 5.8 5.8 15 15 15
y6 (1+) 6.6 2.7 3 12.2 3.6 4.1 13.9 4.5 5.1 15 15 15
y7 (1+) 10.7 4.2 2.9 14.4 4.2 3.5 17.9 5.9 4.5 15 15 15
sum 3.8 1.3 1.3 7.7 3 3.6 8.6 3.3 3.8 15 15 15


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