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Overview Data source: UniProt

Official Gene Symbol Other Aliases
SNCG BCSG1, PERSYN, PRSN
Sequence Length (AA) Molecular Weight (Da)
127 13331
Protein Name
Gamma-synuclein
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MDVFKKGFSI AKEGVVGAVE KTKQGVTEAA EKTKEGVMYV GAKTKENVVQ
60 70 80 90 100
SVTSVAEKTK EQANAVSEAV VSSVNTVATK TVEEAENIAV TSGVVRKEDL
110 120 127
RPSAPQQEGE ASKEKEEVAE EAQSGGD

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node
to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1459 Collapse assay details

Data source: Panorama

Official Gene Symbol
SNCG
Peptide Sequence
EGVVGAVEK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
13
Peptide End
21
CPTAC ID
CPTAC-1459
Peptide Molecular Mass
886.4760
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
depleted digested plasma
Submitting Laboratory
Broad Institute
Submitting Lab PI
Steven A. Carr

Publication

View Details (opens in a new window)

Large-Scale Interlaboratory Study to Develop, Analytically Validate and Apply Highly Multiplexed, Quantitative Peptide Assays to Measure Cancer-Relevant Proteins in Plasma. Abbatiello SE, Schilling B, Mani DR, Zimmerman LJ, Hall SC, MacLean B, Albertolle M, Allen S, Burgess M, Cusack MP, Gosh M, Hedrick V, Held JM, Inerowicz HD, Jackson A, Keshishian H, Kinsinger CR, Lyssand J, Makowski L, Mesri M, Rodriguez H, Rudnick P, Sadowski P, Sedransk N, Shaddox K, Skates SJ, Kuhn E, Smith D, Whiteaker JR, Whitwell C, Zhang S, Borchers CH, Fisher SJ, Gibson BW, Liebler DC, MacCoss MJ, Neubert TA, Paulovich AG, Regnier FE, Tempst P, Carr SA. Mol Cell Proteomics. 2015 Sep;14(9):2357-74. doi: 10.1074/mcp.M114.047050. Epub 2015 Feb 18. PMID: 25693799


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Quantiva TSQ
Internal Standard
heavy stable isotope peptide (delta 8-10)
Peptide Standard Purity
>95%
Peptide Standard Label Type
15N
LC
Easy NanoLC1000
Column Packing
Reprosil C18, 1.9um, 200A
Column Dimensions
0.075 x 150 mm
Flow Rate
200 nL/min

Assay Multiplexing Expand assay panel

Broad Institute-Plasma-based Candidate Cancer Biomarkers

CPTAC-1372:
ALDOC.
ALQASALNAWR
CPTAC-1373:
ALDOC.
AEVNGLAAQGK
CPTAC-1374:
ALDOC.
ELSDIALR
CPTAC-1375:
ALDOC.
TPSALAILENANVLAR
CPTAC-1376:
ALDOC.
QVLFSADDR
CPTAC-1377:
ANXA1.
ALYEAGER
CPTAC-1378:
ANXA1.
GTDVNVFNTILTTR
CPTAC-1379:
ANXA1.
AAYLQETGKPLDETLK
CPTAC-1380:
ANXA1.
TPAQFDADELR
CPTAC-1381:
ANXA4.
VLVSLSAGGR
CPTAC-1382:
ANXA4.
GLGTDEDAIISVLAYR
CPTAC-1383:
ANXA4.
DEGNYLDDALVR
CPTAC-1384:
ANXA4.
GAGTDEGC[+57.0]LIEILASR
CPTAC-1385:
ANXA7.
LYQAGEGR
CPTAC-1386:
ANXA7.
SEIDLVQIK
CPTAC-1387:
ANXA7.
GAGTDDSTLVR
CPTAC-1388:
ANXA7.
EFSGYVESGLK
CPTAC-1389:
ANXA7.
GFGTDEQAIVDVVANR
CPTAC-1390:
CALR.
QIDNPDYK
CPTAC-1391:
CALR.
FYALSASFEPFSNK
CPTAC-1392:
CALR.
EQFLDGDGWTSR
CPTAC-1393:
CLIC1.
LHIVQVVC[+57.0]K
CPTAC-1394:
CLIC1.
YLSNAYAR
CPTAC-1395:
CLIC1.
IEEFLEAVLC[+57.0]PPR
CPTAC-1396:
CLIC1.
GVTFNVTTVDTK
CPTAC-1397:
CLIC1.
GFTIPEAFR
CPTAC-1398:
EZR.
IQVWHAEHR
CPTAC-1399:
EZR.
EDEVEEWQHR
CPTAC-1400:
EZR.
SGYLSSER
CPTAC-1401:
EZR.
IALLEEAR
CPTAC-1402:
EZR.
SQEQLAAELAEYTAK
CPTAC-1403:
FSCN1.
FLIVAHDDGR
CPTAC-1404:
FSCN1.
YLAPSGPSGTLK
CPTAC-1405:
FSCN1.
VTGTLDANR
CPTAC-1406:
FSCN1.
LSC[+57.0]FAQTVSPAEK
CPTAC-1407:
FTL.
LGGPEAGLGEYLFER
CPTAC-1408:
FTL.
KPAEDEWGK
CPTAC-1409:
FEN1.
SIEEIVR
CPTAC-1410:
FEN1.
QLQQAQAAGAEQEVEK
CPTAC-1411:
FEN1.
LIADVAPSAIR
CPTAC-1412:
GSTP1.
FQDGDLTLYQSNTILR
CPTAC-1413:
GSTP1.
EEVVTVETWQEGSLK
CPTAC-1414:
GSTP1.
PPYTVVYFPVR
CPTAC-1415:
GSTP1.
ASC[+57.0]LYGQLPK
CPTAC-1416:
GLO1.
FSLYFLAYEDK
CPTAC-1417:
GLO1.
SLDFYTR
CPTAC-1418:
GLO1.
IAWALSR
CPTAC-1419:
GLO1.
GFGHIGIAVPDVYSAC[+57.0]K
CPTAC-1420:
GLO1.
FEELGVK
CPTAC-1421:
GRB2.
FNSLNELVDYHR
CPTAC-1422:
GRB2.
FGNDVQHFK
CPTAC-1423:
GRB2.
NYVTPVNR
CPTAC-1424:
GRB2.
ATADDELSFK
CPTAC-1425:
GRB2.
ESESAPGDFSLSVK
CPTAC-1426:
HSPB1.
LFDQAFGLPR
CPTAC-1427:
HSPB1.
AQLGGPEAAK
CPTAC-1428:
HSPB1.
VSLDVNHFAPDELTVK
CPTAC-1429:
HSPB1.
DGVVEITGK
CPTAC-1430:
HSPB1.
QLSSGVSEIR
CPTAC-1431:
IL18.
EDELGDR
CPTAC-1432:
IL18.
SDIIFFQR
CPTAC-1433:
IL18.
TIFIISMYK
CPTAC-1434:
IL18.
ISTLSC[+57.0]ENK
CPTAC-1435:
IL18.
GMAVTISVK
CPTAC-1436:
LGALS1.
DGGAWGTEQR
CPTAC-1437:
LGALS1.
DSNNLC[+57.0]LHFNPR
CPTAC-1438:
LGALS1.
FNAHGDANTIVC[+57.0]NSK
CPTAC-1439:
LGALS1.
SFVLNLGK
CPTAC-1440:
LGALS1.
LPDGYEFK
CPTAC-1441:
PDLIM1.
C[+57.0]GTGIVGVFVK
CPTAC-1442:
PDLIM1.
VAASIGNAQK
CPTAC-1443:
PDLIM1.
VWSPLVTEEGK
CPTAC-1444:
PDLIM1.
DFEQPLAISR
CPTAC-1445:
PRDX2.
GLFIIDGK
CPTAC-1446:
PRDX2.
TDEGIAYR
CPTAC-1447:
PRDX2.
ATAVVDGAFK
CPTAC-1448:
PRDX4.
DYGVYLEDSGHTLR
CPTAC-1449:
PRDX4.
LVQAFQYTDK
CPTAC-1450:
PRDX4.
IPLLSDLTHQISK
CPTAC-1451:
PRDX4.
QITLNDLPVGR
CPTAC-1452:
S100A1.
ELLQTELSGFLDAQK
CPTAC-1453:
S100A2.
ELPSFVGEK
CPTAC-1454:
S100B.
AMVALIDVFHQYSGR
CPTAC-1455:
S100B.
ELINNELSHFLEEIK
CPTAC-1456:
RAD23B.
EQVIAALR
CPTAC-1457:
RAD23B.
IDIDPEETVK
CPTAC-1458:
RAD23B.
ILNDDTALK
CPTAC-1459:
SNCG.
EGVVGAVEK
CPTAC-1460:
SNCG.
ENVVQSVTSVAEK
CPTAC-1461:
SNCG.
TVEEAENIAVTSGVVR
CPTAC-1462:
TPM1.
LVIIESDLER
CPTAC-1463:
TPM1.
SIDDLEDELYAQK
CPTAC-1464:
TPM1.
QLEDELVSLQK
CPTAC-1465:
UBE2C.
YLQETYSK
CPTAC-1466:
UBE2C.
LSLEFPSGYPYNAPTVK
CPTAC-1467:
UBE2C.
DPAATSVAAAR
CPTAC-1468:
UBE2C.
GISAFPESDNLFK
CPTAC-1469:
UBE2I.
DDYPSSPPK
CPTAC-1470:
UBE2I.
DWRPAITIK
CPTAC-1471:
UBE2I.
DHPFGFVAVPTK

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (1+) 2.1 1.6 1.1 3.3 6.4 2.8 3.9 6.6 3 15 15 15
y6 (1+) 2.7 2.3 1.1 3.1 6.1 2.6 4.1 6.5 2.8 15 15 15
y4 (1+) 5.8 2 1.8 6.5 6.3 3.3 8.7 6.6 3.8 15 15 15
sum 1.8 1.5 0.9 2.9 6.3 2.6 3.4 6.5 2.8 15 15 15

Additional Resources and Comments


Assay Details for CPTAC-1460 Collapse assay details

Data source: Panorama

Official Gene Symbol
SNCG
Peptide Sequence
ENVVQSVTSVAEK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
46
Peptide End
58
CPTAC ID
CPTAC-1460
Peptide Molecular Mass
1,388.7147
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
depleted digested plasma
Submitting Laboratory
Broad Institute
Submitting Lab PI
Steven A. Carr

Publication

View Details (opens in a new window)

Large-Scale Interlaboratory Study to Develop, Analytically Validate and Apply Highly Multiplexed, Quantitative Peptide Assays to Measure Cancer-Relevant Proteins in Plasma. Abbatiello SE, Schilling B, Mani DR, Zimmerman LJ, Hall SC, MacLean B, Albertolle M, Allen S, Burgess M, Cusack MP, Gosh M, Hedrick V, Held JM, Inerowicz HD, Jackson A, Keshishian H, Kinsinger CR, Lyssand J, Makowski L, Mesri M, Rodriguez H, Rudnick P, Sadowski P, Sedransk N, Shaddox K, Skates SJ, Kuhn E, Smith D, Whiteaker JR, Whitwell C, Zhang S, Borchers CH, Fisher SJ, Gibson BW, Liebler DC, MacCoss MJ, Neubert TA, Paulovich AG, Regnier FE, Tempst P, Carr SA. Mol Cell Proteomics. 2015 Sep;14(9):2357-74. doi: 10.1074/mcp.M114.047050. Epub 2015 Feb 18. PMID: 25693799


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Quantiva TSQ
Internal Standard
heavy stable isotope peptide (delta 8-10)
Peptide Standard Purity
>95%
Peptide Standard Label Type
15N
LC
Easy NanoLC1000
Column Packing
Reprosil C18, 1.9um, 200A
Column Dimensions
0.075 x 150 mm
Flow Rate
200 nL/min

Assay Multiplexing Expand assay panel

Broad Institute-Plasma-based Candidate Cancer Biomarkers

CPTAC-1372:
ALDOC.
ALQASALNAWR
CPTAC-1373:
ALDOC.
AEVNGLAAQGK
CPTAC-1374:
ALDOC.
ELSDIALR
CPTAC-1375:
ALDOC.
TPSALAILENANVLAR
CPTAC-1376:
ALDOC.
QVLFSADDR
CPTAC-1377:
ANXA1.
ALYEAGER
CPTAC-1378:
ANXA1.
GTDVNVFNTILTTR
CPTAC-1379:
ANXA1.
AAYLQETGKPLDETLK
CPTAC-1380:
ANXA1.
TPAQFDADELR
CPTAC-1381:
ANXA4.
VLVSLSAGGR
CPTAC-1382:
ANXA4.
GLGTDEDAIISVLAYR
CPTAC-1383:
ANXA4.
DEGNYLDDALVR
CPTAC-1384:
ANXA4.
GAGTDEGC[+57.0]LIEILASR
CPTAC-1385:
ANXA7.
LYQAGEGR
CPTAC-1386:
ANXA7.
SEIDLVQIK
CPTAC-1387:
ANXA7.
GAGTDDSTLVR
CPTAC-1388:
ANXA7.
EFSGYVESGLK
CPTAC-1389:
ANXA7.
GFGTDEQAIVDVVANR
CPTAC-1390:
CALR.
QIDNPDYK
CPTAC-1391:
CALR.
FYALSASFEPFSNK
CPTAC-1392:
CALR.
EQFLDGDGWTSR
CPTAC-1393:
CLIC1.
LHIVQVVC[+57.0]K
CPTAC-1394:
CLIC1.
YLSNAYAR
CPTAC-1395:
CLIC1.
IEEFLEAVLC[+57.0]PPR
CPTAC-1396:
CLIC1.
GVTFNVTTVDTK
CPTAC-1397:
CLIC1.
GFTIPEAFR
CPTAC-1398:
EZR.
IQVWHAEHR
CPTAC-1399:
EZR.
EDEVEEWQHR
CPTAC-1400:
EZR.
SGYLSSER
CPTAC-1401:
EZR.
IALLEEAR
CPTAC-1402:
EZR.
SQEQLAAELAEYTAK
CPTAC-1403:
FSCN1.
FLIVAHDDGR
CPTAC-1404:
FSCN1.
YLAPSGPSGTLK
CPTAC-1405:
FSCN1.
VTGTLDANR
CPTAC-1406:
FSCN1.
LSC[+57.0]FAQTVSPAEK
CPTAC-1407:
FTL.
LGGPEAGLGEYLFER
CPTAC-1408:
FTL.
KPAEDEWGK
CPTAC-1409:
FEN1.
SIEEIVR
CPTAC-1410:
FEN1.
QLQQAQAAGAEQEVEK
CPTAC-1411:
FEN1.
LIADVAPSAIR
CPTAC-1412:
GSTP1.
FQDGDLTLYQSNTILR
CPTAC-1413:
GSTP1.
EEVVTVETWQEGSLK
CPTAC-1414:
GSTP1.
PPYTVVYFPVR
CPTAC-1415:
GSTP1.
ASC[+57.0]LYGQLPK
CPTAC-1416:
GLO1.
FSLYFLAYEDK
CPTAC-1417:
GLO1.
SLDFYTR
CPTAC-1418:
GLO1.
IAWALSR
CPTAC-1419:
GLO1.
GFGHIGIAVPDVYSAC[+57.0]K
CPTAC-1420:
GLO1.
FEELGVK
CPTAC-1421:
GRB2.
FNSLNELVDYHR
CPTAC-1422:
GRB2.
FGNDVQHFK
CPTAC-1423:
GRB2.
NYVTPVNR
CPTAC-1424:
GRB2.
ATADDELSFK
CPTAC-1425:
GRB2.
ESESAPGDFSLSVK
CPTAC-1426:
HSPB1.
LFDQAFGLPR
CPTAC-1427:
HSPB1.
AQLGGPEAAK
CPTAC-1428:
HSPB1.
VSLDVNHFAPDELTVK
CPTAC-1429:
HSPB1.
DGVVEITGK
CPTAC-1430:
HSPB1.
QLSSGVSEIR
CPTAC-1431:
IL18.
EDELGDR
CPTAC-1432:
IL18.
SDIIFFQR
CPTAC-1433:
IL18.
TIFIISMYK
CPTAC-1434:
IL18.
ISTLSC[+57.0]ENK
CPTAC-1435:
IL18.
GMAVTISVK
CPTAC-1436:
LGALS1.
DGGAWGTEQR
CPTAC-1437:
LGALS1.
DSNNLC[+57.0]LHFNPR
CPTAC-1438:
LGALS1.
FNAHGDANTIVC[+57.0]NSK
CPTAC-1439:
LGALS1.
SFVLNLGK
CPTAC-1440:
LGALS1.
LPDGYEFK
CPTAC-1441:
PDLIM1.
C[+57.0]GTGIVGVFVK
CPTAC-1442:
PDLIM1.
VAASIGNAQK
CPTAC-1443:
PDLIM1.
VWSPLVTEEGK
CPTAC-1444:
PDLIM1.
DFEQPLAISR
CPTAC-1445:
PRDX2.
GLFIIDGK
CPTAC-1446:
PRDX2.
TDEGIAYR
CPTAC-1447:
PRDX2.
ATAVVDGAFK
CPTAC-1448:
PRDX4.
DYGVYLEDSGHTLR
CPTAC-1449:
PRDX4.
LVQAFQYTDK
CPTAC-1450:
PRDX4.
IPLLSDLTHQISK
CPTAC-1451:
PRDX4.
QITLNDLPVGR
CPTAC-1452:
S100A1.
ELLQTELSGFLDAQK
CPTAC-1453:
S100A2.
ELPSFVGEK
CPTAC-1454:
S100B.
AMVALIDVFHQYSGR
CPTAC-1455:
S100B.
ELINNELSHFLEEIK
CPTAC-1456:
RAD23B.
EQVIAALR
CPTAC-1457:
RAD23B.
IDIDPEETVK
CPTAC-1458:
RAD23B.
ILNDDTALK
CPTAC-1459:
SNCG.
EGVVGAVEK
CPTAC-1460:
SNCG.
ENVVQSVTSVAEK
CPTAC-1461:
SNCG.
TVEEAENIAVTSGVVR
CPTAC-1462:
TPM1.
LVIIESDLER
CPTAC-1463:
TPM1.
SIDDLEDELYAQK
CPTAC-1464:
TPM1.
QLEDELVSLQK
CPTAC-1465:
UBE2C.
YLQETYSK
CPTAC-1466:
UBE2C.
LSLEFPSGYPYNAPTVK
CPTAC-1467:
UBE2C.
DPAATSVAAAR
CPTAC-1468:
UBE2C.
GISAFPESDNLFK
CPTAC-1469:
UBE2I.
DDYPSSPPK
CPTAC-1470:
UBE2I.
DWRPAITIK
CPTAC-1471:
UBE2I.
DHPFGFVAVPTK

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y10 (1+) 6.9 2.8 2.2 8.7 6.1 4.3 11.1 6.7 4.8 15 15 15
y8 (1+) 3.8 2.5 2.1 5.5 5.4 3.1 6.7 6 3.7 15 15 15
y9 (1+) 4.8 2.3 2.1 6.2 6.3 3.2 7.8 6.7 3.8 15 15 15
sum 2.4 2 1.5 5.4 5.7 3 5.9 6 3.4 15 15 15

Additional Resources and Comments


Assay Details for CPTAC-1461 Collapse assay details

Data source: Panorama

Official Gene Symbol
SNCG
Peptide Sequence
TVEEAENIAVTSGVVR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
81
Peptide End
96
CPTAC ID
CPTAC-1461
Peptide Molecular Mass
1,672.8632
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
depleted digested plasma
Submitting Laboratory
Broad Institute
Submitting Lab PI
Steven A. Carr

Publication

View Details (opens in a new window)

Large-Scale Interlaboratory Study to Develop, Analytically Validate and Apply Highly Multiplexed, Quantitative Peptide Assays to Measure Cancer-Relevant Proteins in Plasma. Abbatiello SE, Schilling B, Mani DR, Zimmerman LJ, Hall SC, MacLean B, Albertolle M, Allen S, Burgess M, Cusack MP, Gosh M, Hedrick V, Held JM, Inerowicz HD, Jackson A, Keshishian H, Kinsinger CR, Lyssand J, Makowski L, Mesri M, Rodriguez H, Rudnick P, Sadowski P, Sedransk N, Shaddox K, Skates SJ, Kuhn E, Smith D, Whiteaker JR, Whitwell C, Zhang S, Borchers CH, Fisher SJ, Gibson BW, Liebler DC, MacCoss MJ, Neubert TA, Paulovich AG, Regnier FE, Tempst P, Carr SA. Mol Cell Proteomics. 2015 Sep;14(9):2357-74. doi: 10.1074/mcp.M114.047050. Epub 2015 Feb 18. PMID: 25693799


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Quantiva TSQ
Internal Standard
heavy stable isotope peptide (delta 8-10)
Peptide Standard Purity
>95%
Peptide Standard Label Type
15N
LC
Easy NanoLC1000
Column Packing
Reprosil C18, 1.9um, 200A
Column Dimensions
0.075 x 150 mm
Flow Rate
200 nL/min

Assay Multiplexing Expand assay panel

Broad Institute-Plasma-based Candidate Cancer Biomarkers

CPTAC-1372:
ALDOC.
ALQASALNAWR
CPTAC-1373:
ALDOC.
AEVNGLAAQGK
CPTAC-1374:
ALDOC.
ELSDIALR
CPTAC-1375:
ALDOC.
TPSALAILENANVLAR
CPTAC-1376:
ALDOC.
QVLFSADDR
CPTAC-1377:
ANXA1.
ALYEAGER
CPTAC-1378:
ANXA1.
GTDVNVFNTILTTR
CPTAC-1379:
ANXA1.
AAYLQETGKPLDETLK
CPTAC-1380:
ANXA1.
TPAQFDADELR
CPTAC-1381:
ANXA4.
VLVSLSAGGR
CPTAC-1382:
ANXA4.
GLGTDEDAIISVLAYR
CPTAC-1383:
ANXA4.
DEGNYLDDALVR
CPTAC-1384:
ANXA4.
GAGTDEGC[+57.0]LIEILASR
CPTAC-1385:
ANXA7.
LYQAGEGR
CPTAC-1386:
ANXA7.
SEIDLVQIK
CPTAC-1387:
ANXA7.
GAGTDDSTLVR
CPTAC-1388:
ANXA7.
EFSGYVESGLK
CPTAC-1389:
ANXA7.
GFGTDEQAIVDVVANR
CPTAC-1390:
CALR.
QIDNPDYK
CPTAC-1391:
CALR.
FYALSASFEPFSNK
CPTAC-1392:
CALR.
EQFLDGDGWTSR
CPTAC-1393:
CLIC1.
LHIVQVVC[+57.0]K
CPTAC-1394:
CLIC1.
YLSNAYAR
CPTAC-1395:
CLIC1.
IEEFLEAVLC[+57.0]PPR
CPTAC-1396:
CLIC1.
GVTFNVTTVDTK
CPTAC-1397:
CLIC1.
GFTIPEAFR
CPTAC-1398:
EZR.
IQVWHAEHR
CPTAC-1399:
EZR.
EDEVEEWQHR
CPTAC-1400:
EZR.
SGYLSSER
CPTAC-1401:
EZR.
IALLEEAR
CPTAC-1402:
EZR.
SQEQLAAELAEYTAK
CPTAC-1403:
FSCN1.
FLIVAHDDGR
CPTAC-1404:
FSCN1.
YLAPSGPSGTLK
CPTAC-1405:
FSCN1.
VTGTLDANR
CPTAC-1406:
FSCN1.
LSC[+57.0]FAQTVSPAEK
CPTAC-1407:
FTL.
LGGPEAGLGEYLFER
CPTAC-1408:
FTL.
KPAEDEWGK
CPTAC-1409:
FEN1.
SIEEIVR
CPTAC-1410:
FEN1.
QLQQAQAAGAEQEVEK
CPTAC-1411:
FEN1.
LIADVAPSAIR
CPTAC-1412:
GSTP1.
FQDGDLTLYQSNTILR
CPTAC-1413:
GSTP1.
EEVVTVETWQEGSLK
CPTAC-1414:
GSTP1.
PPYTVVYFPVR
CPTAC-1415:
GSTP1.
ASC[+57.0]LYGQLPK
CPTAC-1416:
GLO1.
FSLYFLAYEDK
CPTAC-1417:
GLO1.
SLDFYTR
CPTAC-1418:
GLO1.
IAWALSR
CPTAC-1419:
GLO1.
GFGHIGIAVPDVYSAC[+57.0]K
CPTAC-1420:
GLO1.
FEELGVK
CPTAC-1421:
GRB2.
FNSLNELVDYHR
CPTAC-1422:
GRB2.
FGNDVQHFK
CPTAC-1423:
GRB2.
NYVTPVNR
CPTAC-1424:
GRB2.
ATADDELSFK
CPTAC-1425:
GRB2.
ESESAPGDFSLSVK
CPTAC-1426:
HSPB1.
LFDQAFGLPR
CPTAC-1427:
HSPB1.
AQLGGPEAAK
CPTAC-1428:
HSPB1.
VSLDVNHFAPDELTVK
CPTAC-1429:
HSPB1.
DGVVEITGK
CPTAC-1430:
HSPB1.
QLSSGVSEIR
CPTAC-1431:
IL18.
EDELGDR
CPTAC-1432:
IL18.
SDIIFFQR
CPTAC-1433:
IL18.
TIFIISMYK
CPTAC-1434:
IL18.
ISTLSC[+57.0]ENK
CPTAC-1435:
IL18.
GMAVTISVK
CPTAC-1436:
LGALS1.
DGGAWGTEQR
CPTAC-1437:
LGALS1.
DSNNLC[+57.0]LHFNPR
CPTAC-1438:
LGALS1.
FNAHGDANTIVC[+57.0]NSK
CPTAC-1439:
LGALS1.
SFVLNLGK
CPTAC-1440:
LGALS1.
LPDGYEFK
CPTAC-1441:
PDLIM1.
C[+57.0]GTGIVGVFVK
CPTAC-1442:
PDLIM1.
VAASIGNAQK
CPTAC-1443:
PDLIM1.
VWSPLVTEEGK
CPTAC-1444:
PDLIM1.
DFEQPLAISR
CPTAC-1445:
PRDX2.
GLFIIDGK
CPTAC-1446:
PRDX2.
TDEGIAYR
CPTAC-1447:
PRDX2.
ATAVVDGAFK
CPTAC-1448:
PRDX4.
DYGVYLEDSGHTLR
CPTAC-1449:
PRDX4.
LVQAFQYTDK
CPTAC-1450:
PRDX4.
IPLLSDLTHQISK
CPTAC-1451:
PRDX4.
QITLNDLPVGR
CPTAC-1452:
S100A1.
ELLQTELSGFLDAQK
CPTAC-1453:
S100A2.
ELPSFVGEK
CPTAC-1454:
S100B.
AMVALIDVFHQYSGR
CPTAC-1455:
S100B.
ELINNELSHFLEEIK
CPTAC-1456:
RAD23B.
EQVIAALR
CPTAC-1457:
RAD23B.
IDIDPEETVK
CPTAC-1458:
RAD23B.
ILNDDTALK
CPTAC-1459:
SNCG.
EGVVGAVEK
CPTAC-1460:
SNCG.
ENVVQSVTSVAEK
CPTAC-1461:
SNCG.
TVEEAENIAVTSGVVR
CPTAC-1462:
TPM1.
LVIIESDLER
CPTAC-1463:
TPM1.
SIDDLEDELYAQK
CPTAC-1464:
TPM1.
QLEDELVSLQK
CPTAC-1465:
UBE2C.
YLQETYSK
CPTAC-1466:
UBE2C.
LSLEFPSGYPYNAPTVK
CPTAC-1467:
UBE2C.
DPAATSVAAAR
CPTAC-1468:
UBE2C.
GISAFPESDNLFK
CPTAC-1469:
UBE2I.
DDYPSSPPK
CPTAC-1470:
UBE2I.
DWRPAITIK
CPTAC-1471:
UBE2I.
DHPFGFVAVPTK

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 13.7 6.5 6.5 14.3 9.4 6 19.8 11.4 8.8 15 15 15
y5 (1+) 17.5 5.5 5.2 26.4 8.5 4.9 31.7 10.1 7.1 15 15 15
y7 (1+) 14 7.7 5.9 17.5 13.5 6.6 22.4 15.5 8.9 15 15 15
sum 9.2 4.1 4.2 11.2 8.5 4.7 14.5 9.4 6.3 15 15 15

Additional Resources and Comments


Assay Details for CPTAC-133 Collapse assay details

Data source: Panorama

Official Gene Symbol
SNCG
Peptide Sequence
TVEEAENIAVTSGVVR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
81
Peptide End
96
CPTAC ID
CPTAC-133
Peptide Molecular Mass
1,672.8632
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Assay Multiplexing Expand assay panel

Broad Institute-Plasma-based Candidate Cancer Biomarkers

CPTAC-1372:
ALDOC.
ALQASALNAWR
CPTAC-1373:
ALDOC.
AEVNGLAAQGK
CPTAC-1374:
ALDOC.
ELSDIALR
CPTAC-1375:
ALDOC.
TPSALAILENANVLAR
CPTAC-1376:
ALDOC.
QVLFSADDR
CPTAC-1377:
ANXA1.
ALYEAGER
CPTAC-1378:
ANXA1.
GTDVNVFNTILTTR
CPTAC-1379:
ANXA1.
AAYLQETGKPLDETLK
CPTAC-1380:
ANXA1.
TPAQFDADELR
CPTAC-1381:
ANXA4.
VLVSLSAGGR
CPTAC-1382:
ANXA4.
GLGTDEDAIISVLAYR
CPTAC-1383:
ANXA4.
DEGNYLDDALVR
CPTAC-1384:
ANXA4.
GAGTDEGC[+57.0]LIEILASR
CPTAC-1385:
ANXA7.
LYQAGEGR
CPTAC-1386:
ANXA7.
SEIDLVQIK
CPTAC-1387:
ANXA7.
GAGTDDSTLVR
CPTAC-1388:
ANXA7.
EFSGYVESGLK
CPTAC-1389:
ANXA7.
GFGTDEQAIVDVVANR
CPTAC-1390:
CALR.
QIDNPDYK
CPTAC-1391:
CALR.
FYALSASFEPFSNK
CPTAC-1392:
CALR.
EQFLDGDGWTSR
CPTAC-1393:
CLIC1.
LHIVQVVC[+57.0]K
CPTAC-1394:
CLIC1.
YLSNAYAR
CPTAC-1395:
CLIC1.
IEEFLEAVLC[+57.0]PPR
CPTAC-1396:
CLIC1.
GVTFNVTTVDTK
CPTAC-1397:
CLIC1.
GFTIPEAFR
CPTAC-1398:
EZR.
IQVWHAEHR
CPTAC-1399:
EZR.
EDEVEEWQHR
CPTAC-1400:
EZR.
SGYLSSER
CPTAC-1401:
EZR.
IALLEEAR
CPTAC-1402:
EZR.
SQEQLAAELAEYTAK
CPTAC-1403:
FSCN1.
FLIVAHDDGR
CPTAC-1404:
FSCN1.
YLAPSGPSGTLK
CPTAC-1405:
FSCN1.
VTGTLDANR
CPTAC-1406:
FSCN1.
LSC[+57.0]FAQTVSPAEK
CPTAC-1407:
FTL.
LGGPEAGLGEYLFER
CPTAC-1408:
FTL.
KPAEDEWGK
CPTAC-1409:
FEN1.
SIEEIVR
CPTAC-1410:
FEN1.
QLQQAQAAGAEQEVEK
CPTAC-1411:
FEN1.
LIADVAPSAIR
CPTAC-1412:
GSTP1.
FQDGDLTLYQSNTILR
CPTAC-1413:
GSTP1.
EEVVTVETWQEGSLK
CPTAC-1414:
GSTP1.
PPYTVVYFPVR
CPTAC-1415:
GSTP1.
ASC[+57.0]LYGQLPK
CPTAC-1416:
GLO1.
FSLYFLAYEDK
CPTAC-1417:
GLO1.
SLDFYTR
CPTAC-1418:
GLO1.
IAWALSR
CPTAC-1419:
GLO1.
GFGHIGIAVPDVYSAC[+57.0]K
CPTAC-1420:
GLO1.
FEELGVK
CPTAC-1421:
GRB2.
FNSLNELVDYHR
CPTAC-1422:
GRB2.
FGNDVQHFK
CPTAC-1423:
GRB2.
NYVTPVNR
CPTAC-1424:
GRB2.
ATADDELSFK
CPTAC-1425:
GRB2.
ESESAPGDFSLSVK
CPTAC-1426:
HSPB1.
LFDQAFGLPR
CPTAC-1427:
HSPB1.
AQLGGPEAAK
CPTAC-1428:
HSPB1.
VSLDVNHFAPDELTVK
CPTAC-1429:
HSPB1.
DGVVEITGK
CPTAC-1430:
HSPB1.
QLSSGVSEIR
CPTAC-1431:
IL18.
EDELGDR
CPTAC-1432:
IL18.
SDIIFFQR
CPTAC-1433:
IL18.
TIFIISMYK
CPTAC-1434:
IL18.
ISTLSC[+57.0]ENK
CPTAC-1435:
IL18.
GMAVTISVK
CPTAC-1436:
LGALS1.
DGGAWGTEQR
CPTAC-1437:
LGALS1.
DSNNLC[+57.0]LHFNPR
CPTAC-1438:
LGALS1.
FNAHGDANTIVC[+57.0]NSK
CPTAC-1439:
LGALS1.
SFVLNLGK
CPTAC-1440:
LGALS1.
LPDGYEFK
CPTAC-1441:
PDLIM1.
C[+57.0]GTGIVGVFVK
CPTAC-1442:
PDLIM1.
VAASIGNAQK
CPTAC-1443:
PDLIM1.
VWSPLVTEEGK
CPTAC-1444:
PDLIM1.
DFEQPLAISR
CPTAC-1445:
PRDX2.
GLFIIDGK
CPTAC-1446:
PRDX2.
TDEGIAYR
CPTAC-1447:
PRDX2.
ATAVVDGAFK
CPTAC-1448:
PRDX4.
DYGVYLEDSGHTLR
CPTAC-1449:
PRDX4.
LVQAFQYTDK
CPTAC-1450:
PRDX4.
IPLLSDLTHQISK
CPTAC-1451:
PRDX4.
QITLNDLPVGR
CPTAC-1452:
S100A1.
ELLQTELSGFLDAQK
CPTAC-1453:
S100A2.
ELPSFVGEK
CPTAC-1454:
S100B.
AMVALIDVFHQYSGR
CPTAC-1455:
S100B.
ELINNELSHFLEEIK
CPTAC-1456:
RAD23B.
EQVIAALR
CPTAC-1457:
RAD23B.
IDIDPEETVK
CPTAC-1458:
RAD23B.
ILNDDTALK
CPTAC-1459:
SNCG.
EGVVGAVEK
CPTAC-1460:
SNCG.
ENVVQSVTSVAEK
CPTAC-1461:
SNCG.
TVEEAENIAVTSGVVR
CPTAC-1462:
TPM1.
LVIIESDLER
CPTAC-1463:
TPM1.
SIDDLEDELYAQK
CPTAC-1464:
TPM1.
QLEDELVSLQK
CPTAC-1465:
UBE2C.
YLQETYSK
CPTAC-1466:
UBE2C.
LSLEFPSGYPYNAPTVK
CPTAC-1467:
UBE2C.
DPAATSVAAAR
CPTAC-1468:
UBE2C.
GISAFPESDNLFK
CPTAC-1469:
UBE2I.
DDYPSSPPK
CPTAC-1470:
UBE2I.
DWRPAITIK
CPTAC-1471:
UBE2I.
DHPFGFVAVPTK

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y10 (1+) 11.4 9.9 9 19.5 11.6 11 22.6 15.3 14.2 15 25 15
y8 (1+) 10 7 6.2 16.6 7.2 12.2 19.4 10 13.7 15 25 15
y6 (1+) 8.4 6.9 6.8 11 7.8 12.7 13.8 10.4 14.4 15 25 15
sum 6.9 6.7 4.8 13.7 7.3 11.2 15.3 9.9 12.2 15 25 15

Additional Resources and Comments


Assay Details for CPTAC-708 Collapse assay details

Data source: Panorama

Official Gene Symbol
SNCG
Peptide Sequence
TVEEAENIAVTSGVVR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
81
Peptide End
96
CPTAC ID
CPTAC-708
Peptide Molecular Mass
1,672.8632
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
N/A
Matrix
plasma
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Anti-peptide monoclonal antibodies generated for immuno-multiple reaction monitoring-mass spectrometry assays have a high probability of supporting Western blot and ELISA. Schoenherr RM, Saul RG, Whiteaker JR, Yan P, Whiteley GR, Paulovich AG. Mol Cell Proteomics. 2015 Feb;14(2):382-98. doi: 10.1074/mcp.O114.043133. Epub 2014 Dec 15. PMID: 25512614


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 Qtrap
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
15N
LC
Eksigent NanoLC 2D
Column Packing
ReproSil-Pur C18-AQ 3 um
Column Dimensions
100 mm x 75 um ID
Flow Rate
300 nL/min

Assay Multiplexing Expand assay panel

Broad Institute-Plasma-based Candidate Cancer Biomarkers

CPTAC-1372:
ALDOC.
ALQASALNAWR
CPTAC-1373:
ALDOC.
AEVNGLAAQGK
CPTAC-1374:
ALDOC.
ELSDIALR
CPTAC-1375:
ALDOC.
TPSALAILENANVLAR
CPTAC-1376:
ALDOC.
QVLFSADDR
CPTAC-1377:
ANXA1.
ALYEAGER
CPTAC-1378:
ANXA1.
GTDVNVFNTILTTR
CPTAC-1379:
ANXA1.
AAYLQETGKPLDETLK
CPTAC-1380:
ANXA1.
TPAQFDADELR
CPTAC-1381:
ANXA4.
VLVSLSAGGR
CPTAC-1382:
ANXA4.
GLGTDEDAIISVLAYR
CPTAC-1383:
ANXA4.
DEGNYLDDALVR
CPTAC-1384:
ANXA4.
GAGTDEGC[+57.0]LIEILASR
CPTAC-1385:
ANXA7.
LYQAGEGR
CPTAC-1386:
ANXA7.
SEIDLVQIK
CPTAC-1387:
ANXA7.
GAGTDDSTLVR
CPTAC-1388:
ANXA7.
EFSGYVESGLK
CPTAC-1389:
ANXA7.
GFGTDEQAIVDVVANR
CPTAC-1390:
CALR.
QIDNPDYK
CPTAC-1391:
CALR.
FYALSASFEPFSNK
CPTAC-1392:
CALR.
EQFLDGDGWTSR
CPTAC-1393:
CLIC1.
LHIVQVVC[+57.0]K
CPTAC-1394:
CLIC1.
YLSNAYAR
CPTAC-1395:
CLIC1.
IEEFLEAVLC[+57.0]PPR
CPTAC-1396:
CLIC1.
GVTFNVTTVDTK
CPTAC-1397:
CLIC1.
GFTIPEAFR
CPTAC-1398:
EZR.
IQVWHAEHR
CPTAC-1399:
EZR.
EDEVEEWQHR
CPTAC-1400:
EZR.
SGYLSSER
CPTAC-1401:
EZR.
IALLEEAR
CPTAC-1402:
EZR.
SQEQLAAELAEYTAK
CPTAC-1403:
FSCN1.
FLIVAHDDGR
CPTAC-1404:
FSCN1.
YLAPSGPSGTLK
CPTAC-1405:
FSCN1.
VTGTLDANR
CPTAC-1406:
FSCN1.
LSC[+57.0]FAQTVSPAEK
CPTAC-1407:
FTL.
LGGPEAGLGEYLFER
CPTAC-1408:
FTL.
KPAEDEWGK
CPTAC-1409:
FEN1.
SIEEIVR
CPTAC-1410:
FEN1.
QLQQAQAAGAEQEVEK
CPTAC-1411:
FEN1.
LIADVAPSAIR
CPTAC-1412:
GSTP1.
FQDGDLTLYQSNTILR
CPTAC-1413:
GSTP1.
EEVVTVETWQEGSLK
CPTAC-1414:
GSTP1.
PPYTVVYFPVR
CPTAC-1415:
GSTP1.
ASC[+57.0]LYGQLPK
CPTAC-1416:
GLO1.
FSLYFLAYEDK
CPTAC-1417:
GLO1.
SLDFYTR
CPTAC-1418:
GLO1.
IAWALSR
CPTAC-1419:
GLO1.
GFGHIGIAVPDVYSAC[+57.0]K
CPTAC-1420:
GLO1.
FEELGVK
CPTAC-1421:
GRB2.
FNSLNELVDYHR
CPTAC-1422:
GRB2.
FGNDVQHFK
CPTAC-1423:
GRB2.
NYVTPVNR
CPTAC-1424:
GRB2.
ATADDELSFK
CPTAC-1425:
GRB2.
ESESAPGDFSLSVK
CPTAC-1426:
HSPB1.
LFDQAFGLPR
CPTAC-1427:
HSPB1.
AQLGGPEAAK
CPTAC-1428:
HSPB1.
VSLDVNHFAPDELTVK
CPTAC-1429:
HSPB1.
DGVVEITGK
CPTAC-1430:
HSPB1.
QLSSGVSEIR
CPTAC-1431:
IL18.
EDELGDR
CPTAC-1432:
IL18.
SDIIFFQR
CPTAC-1433:
IL18.
TIFIISMYK
CPTAC-1434:
IL18.
ISTLSC[+57.0]ENK
CPTAC-1435:
IL18.
GMAVTISVK
CPTAC-1436:
LGALS1.
DGGAWGTEQR
CPTAC-1437:
LGALS1.
DSNNLC[+57.0]LHFNPR
CPTAC-1438:
LGALS1.
FNAHGDANTIVC[+57.0]NSK
CPTAC-1439:
LGALS1.
SFVLNLGK
CPTAC-1440:
LGALS1.
LPDGYEFK
CPTAC-1441:
PDLIM1.
C[+57.0]GTGIVGVFVK
CPTAC-1442:
PDLIM1.
VAASIGNAQK
CPTAC-1443:
PDLIM1.
VWSPLVTEEGK
CPTAC-1444:
PDLIM1.
DFEQPLAISR
CPTAC-1445:
PRDX2.
GLFIIDGK
CPTAC-1446:
PRDX2.
TDEGIAYR
CPTAC-1447:
PRDX2.
ATAVVDGAFK
CPTAC-1448:
PRDX4.
DYGVYLEDSGHTLR
CPTAC-1449:
PRDX4.
LVQAFQYTDK
CPTAC-1450:
PRDX4.
IPLLSDLTHQISK
CPTAC-1451:
PRDX4.
QITLNDLPVGR
CPTAC-1452:
S100A1.
ELLQTELSGFLDAQK
CPTAC-1453:
S100A2.
ELPSFVGEK
CPTAC-1454:
S100B.
AMVALIDVFHQYSGR
CPTAC-1455:
S100B.
ELINNELSHFLEEIK
CPTAC-1456:
RAD23B.
EQVIAALR
CPTAC-1457:
RAD23B.
IDIDPEETVK
CPTAC-1458:
RAD23B.
ILNDDTALK
CPTAC-1459:
SNCG.
EGVVGAVEK
CPTAC-1460:
SNCG.
ENVVQSVTSVAEK
CPTAC-1461:
SNCG.
TVEEAENIAVTSGVVR
CPTAC-1462:
TPM1.
LVIIESDLER
CPTAC-1463:
TPM1.
SIDDLEDELYAQK
CPTAC-1464:
TPM1.
QLEDELVSLQK
CPTAC-1465:
UBE2C.
YLQETYSK
CPTAC-1466:
UBE2C.
LSLEFPSGYPYNAPTVK
CPTAC-1467:
UBE2C.
DPAATSVAAAR
CPTAC-1468:
UBE2C.
GISAFPESDNLFK
CPTAC-1469:
UBE2I.
DDYPSSPPK
CPTAC-1470:
UBE2I.
DWRPAITIK
CPTAC-1471:
UBE2I.
DHPFGFVAVPTK

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 5.2 3.3 1.6 7.4 3.3 5.4 9 4.7 5.6 15 15 15
y7 (1+) 6.7 3.9 2.5 8.9 4.3 5.7 11.1 5.8 6.2 15 15 15
y8 (1+) 4.8 2.4 4.7 8.6 3.3 7.9 9.8 4.1 9.2 15 15 15
sum 3.3 2.5 2.8 7 3 6.1 7.7 3.9 6.7 15 15 15

Additional Resources and Comments