| Official Gene Symbol | Other Aliases |
|---|---|
| FSCN1 | FAN1, HSN, SNL |
| Sequence Length (AA) | Molecular Weight (Da) |
|---|---|
| 493 | 54530 |
| Protein Name |
|---|
| Fascin |
| Sources | |
|---|---|
|
UniProt PhosphoSitePlus ® GeneCards |
Human Protein Atlas |
| 10 | 20 | 30 | 40 | 50 |
| MTANGTAEAV | QIQFGLINCG | NKYLTAEAFG | FKVNASASSL | KKKQIWTLEQ |
| 60 | 70 | 80 | 90 | 100 |
| PPDEAGSAAV | CLRSHLGRYL | AADKDGNVTC | EREVPGPDCR | FLIVAHDDGR |
| 110 | 120 | 130 | 140 | 150 |
| WSLQSEAHRR | YFGGTEDRLS | CFAQTVSPAE | KWSVHIAMHP | QVNIYSVTRK |
| 160 | 170 | 180 | 190 | 200 |
| RYAHLSARPA | DEIAVDRDVP | WGVDSLITLA | FQDQRYSVQT | ADHRFLRHDG |
| 210 | 220 | 230 | 240 | 250 |
| RLVARPEPAT | GYTLEFRSGK | VAFRDCEGRY | LAPSGPSGTL | KAGKATKVGK |
| 260 | 270 | 280 | 290 | 300 |
| DELFALEQSC | AQVVLQAANE | RNVSTRQGMD | LSANQDEETD | QETFQLEIDR |
| 310 | 320 | 330 | 340 | 350 |
| DTKKCAFRTH | TGKYWTLTAT | GGVQSTASSK | NASCYFDIEW | RDRRITLRAS |
| 360 | 370 | 380 | 390 | 400 |
| NGKFVTSKKN | GQLAASVETA | GDSELFLMKL | INRPIIVFRG | EHGFIGCRKV |
| 410 | 420 | 430 | 440 | 450 |
| TGTLDANRSS | YDVFQLEFND | GAYNIKDSTG | KYWTVGSDSA | VTSSGDTPVD |
| 460 | 470 | 480 | 490 | 493 |
| FFFEFCDYNK | VAIKVGGRYL | KGDHAGVLKA | SAETVDPASL | WEY |
Data source: UniProt
Uniprot Database Entry PhosphoSitePlus ®
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Data source: Panorama
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Large-Scale Interlaboratory Study to Develop, Analytically Validate and Apply Highly Multiplexed, Quantitative Peptide Assays to Measure Cancer-Relevant Proteins in Plasma. Abbatiello SE, Schilling B, Mani DR, Zimmerman LJ, Hall SC, MacLean B, Albertolle M, Allen S, Burgess M, Cusack MP, Gosh M, Hedrick V, Held JM, Inerowicz HD, Jackson A, Keshishian H, Kinsinger CR, Lyssand J, Makowski L, Mesri M, Rodriguez H, Rudnick P, Sadowski P, Sedransk N, Shaddox K, Skates SJ, Kuhn E, Smith D, Whiteaker JR, Whitwell C, Zhang S, Borchers CH, Fisher SJ, Gibson BW, Liebler DC, MacCoss MJ, Neubert TA, Paulovich AG, Regnier FE, Tempst P, Carr SA. Mol Cell Proteomics. 2015 Sep;14(9):2357-74. doi: 10.1074/mcp.M114.047050. Epub 2015 Feb 18. PMID: 25693799
Data source: Panorama
Broad Institute-Plasma-based Candidate Cancer Biomarkers
Data source: Panorama
Data source: Panorama
Retrieving Data
Data source: Panorama
| Average intra-assay CV (within day CV) |
Average inter-assay CV (between day CV) |
Total CV |
n= | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y8 (2+) | 3.3 | 1 | 1.5 | 3.8 | 5.6 | 2.9 | 5 | 5.7 | 3.3 | 15 | 15 | 15 |
| y7 (2+) | 3 | 2.5 | 3.2 | 5.5 | 6.2 | 4.4 | 6.3 | 6.7 | 5.4 | 15 | 15 | 15 |
| y5 (2+) | 4.5 | 2.4 | 2.7 | 5.1 | 6.1 | 3.7 | 6.8 | 6.6 | 4.6 | 15 | 15 | 15 |
| sum | 2.1 | 1 | 1.6 | 2.4 | 5.6 | 3.1 | 3.2 | 5.7 | 3.5 | 15 | 15 | 15 |
Data source: Panorama
View Details (opens in a new window)
Large-Scale Interlaboratory Study to Develop, Analytically Validate and Apply Highly Multiplexed, Quantitative Peptide Assays to Measure Cancer-Relevant Proteins in Plasma. Abbatiello SE, Schilling B, Mani DR, Zimmerman LJ, Hall SC, MacLean B, Albertolle M, Allen S, Burgess M, Cusack MP, Gosh M, Hedrick V, Held JM, Inerowicz HD, Jackson A, Keshishian H, Kinsinger CR, Lyssand J, Makowski L, Mesri M, Rodriguez H, Rudnick P, Sadowski P, Sedransk N, Shaddox K, Skates SJ, Kuhn E, Smith D, Whiteaker JR, Whitwell C, Zhang S, Borchers CH, Fisher SJ, Gibson BW, Liebler DC, MacCoss MJ, Neubert TA, Paulovich AG, Regnier FE, Tempst P, Carr SA. Mol Cell Proteomics. 2015 Sep;14(9):2357-74. doi: 10.1074/mcp.M114.047050. Epub 2015 Feb 18. PMID: 25693799
Data source: Panorama
Broad Institute-Plasma-based Candidate Cancer Biomarkers
Data source: Panorama
Data source: Panorama
Retrieving Data
Data source: Panorama
| Average intra-assay CV (within day CV) |
Average inter-assay CV (between day CV) |
Total CV |
n= | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y9 (1+) | 4.5 | 2.4 | 1.5 | 5.9 | 5.9 | 4 | 7.4 | 6.4 | 4.3 | 15 | 15 | 15 |
| y7 (1+) | 3.3 | 2.3 | 2.3 | 11.3 | 6.2 | 3.7 | 11.8 | 6.6 | 4.4 | 15 | 15 | 15 |
| y5 (1+) | 2.4 | 2.9 | 1.7 | 5.5 | 6.5 | 2.8 | 6 | 7.1 | 3.3 | 15 | 15 | 15 |
| sum | 1.9 | 2.4 | 1.1 | 6.6 | 6.2 | 2.5 | 6.9 | 6.6 | 2.7 | 15 | 15 | 15 |
Data source: Panorama
View Details (opens in a new window)
Large-Scale Interlaboratory Study to Develop, Analytically Validate and Apply Highly Multiplexed, Quantitative Peptide Assays to Measure Cancer-Relevant Proteins in Plasma. Abbatiello SE, Schilling B, Mani DR, Zimmerman LJ, Hall SC, MacLean B, Albertolle M, Allen S, Burgess M, Cusack MP, Gosh M, Hedrick V, Held JM, Inerowicz HD, Jackson A, Keshishian H, Kinsinger CR, Lyssand J, Makowski L, Mesri M, Rodriguez H, Rudnick P, Sadowski P, Sedransk N, Shaddox K, Skates SJ, Kuhn E, Smith D, Whiteaker JR, Whitwell C, Zhang S, Borchers CH, Fisher SJ, Gibson BW, Liebler DC, MacCoss MJ, Neubert TA, Paulovich AG, Regnier FE, Tempst P, Carr SA. Mol Cell Proteomics. 2015 Sep;14(9):2357-74. doi: 10.1074/mcp.M114.047050. Epub 2015 Feb 18. PMID: 25693799
Data source: Panorama
Broad Institute-Plasma-based Candidate Cancer Biomarkers
Data source: Panorama
Data source: Panorama
Retrieving Data
Data source: Panorama
| Average intra-assay CV (within day CV) |
Average inter-assay CV (between day CV) |
Total CV |
n= | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y7 (1+) | 3.4 | 3.5 | 2.2 | 5 | 5.4 | 4.3 | 6 | 6.4 | 4.8 | 15 | 15 | 15 |
| y5 (1+) | 5 | 3.6 | 2.6 | 6.9 | 6.6 | 4.2 | 8.5 | 7.5 | 4.9 | 15 | 15 | 15 |
| y4 (1+) | 5.1 | 1.4 | 2.4 | 6.2 | 5.7 | 3.4 | 8 | 5.9 | 4.2 | 15 | 15 | 15 |
| sum | 3.3 | 2.6 | 2 | 4.7 | 5.6 | 3.8 | 5.7 | 6.2 | 4.3 | 15 | 15 | 15 |
Data source: Panorama
View Details (opens in a new window)
Large-Scale Interlaboratory Study to Develop, Analytically Validate and Apply Highly Multiplexed, Quantitative Peptide Assays to Measure Cancer-Relevant Proteins in Plasma. Abbatiello SE, Schilling B, Mani DR, Zimmerman LJ, Hall SC, MacLean B, Albertolle M, Allen S, Burgess M, Cusack MP, Gosh M, Hedrick V, Held JM, Inerowicz HD, Jackson A, Keshishian H, Kinsinger CR, Lyssand J, Makowski L, Mesri M, Rodriguez H, Rudnick P, Sadowski P, Sedransk N, Shaddox K, Skates SJ, Kuhn E, Smith D, Whiteaker JR, Whitwell C, Zhang S, Borchers CH, Fisher SJ, Gibson BW, Liebler DC, MacCoss MJ, Neubert TA, Paulovich AG, Regnier FE, Tempst P, Carr SA. Mol Cell Proteomics. 2015 Sep;14(9):2357-74. doi: 10.1074/mcp.M114.047050. Epub 2015 Feb 18. PMID: 25693799
Data source: Panorama
Broad Institute-Plasma-based Candidate Cancer Biomarkers
Data source: Panorama
Data source: Panorama
Retrieving Data
Data source: Panorama
| Average intra-assay CV (within day CV) |
Average inter-assay CV (between day CV) |
Total CV |
n= | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y10 (1+) | 4 | 1.5 | 1.4 | 5.6 | 6.8 | 2.5 | 6.9 | 7 | 2.9 | 15 | 15 | 15 |
| y9 (1+) | 3.1 | 1.6 | 1.3 | 4.7 | 5.2 | 3 | 5.6 | 5.4 | 3.3 | 15 | 15 | 15 |
| y8 (1+) | 3.9 | 2.8 | 2.1 | 4.9 | 7.2 | 2.9 | 6.3 | 7.7 | 3.6 | 15 | 15 | 15 |
| sum | 2.3 | 1.5 | 1.2 | 4.3 | 5.8 | 2.7 | 4.9 | 6 | 3 | 15 | 15 | 15 |
Data source: Panorama
View Details (opens in a new window)
Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253
Response curves acquired at Broad Institute. Repeatability data acquired at Fred Hutchinson Cancer Research Center.
Response curves acquired at Broad Institute. Repeatability data acquired at Fred Hutchinson Cancer Research Center.
Data source: Panorama
Broad Institute-Plasma-based Candidate Cancer Biomarkers
Data source: Panorama
Data source: Panorama
Retrieving Data
Data source: Panorama
| Average intra-assay CV (within day CV) |
Average inter-assay CV (between day CV) |
Total CV |
n= | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y7 (1+) | 8.8 | 10.2 | 7 | 9.5 | 10.2 | 7.3 | 12.9 | 14.4 | 10.1 | 15 | 25 | 15 |
| y5 (1+) | 7.2 | 6.2 | 4.4 | 12.9 | 7.3 | 6 | 14.8 | 9.6 | 7.4 | 15 | 25 | 15 |
| y14 (2+) | 5.2 | 10.8 | 12.7 | 20.2 | 30.4 | 18.5 | 20.9 | 32.3 | 22.4 | 15 | 25 | 15 |
| sum | 4.4 | 7.5 | 7.2 | 12.5 | 13.8 | 9.6 | 13.3 | 15.7 | 12 | 15 | 25 | 15 |
Data source: Panorama
View Details (opens in a new window)
Anti-peptide monoclonal antibodies generated for immuno-multiple reaction monitoring-mass spectrometry assays have a high probability of supporting Western blot and ELISA. Schoenherr RM, Saul RG, Whiteaker JR, Yan P, Whiteley GR, Paulovich AG. Mol Cell Proteomics. 2015 Feb;14(2):382-98. doi: 10.1074/mcp.O114.043133. Epub 2014 Dec 15. PMID: 25512614
Data source: Panorama
Broad Institute-Plasma-based Candidate Cancer Biomarkers
Data source: Panorama
Data source: Panorama
Retrieving Data
Data source: Panorama
| Average intra-assay CV (within day CV) |
Average inter-assay CV (between day CV) |
Total CV |
n= | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y8 (1+) | 16.4 | 3.4 | 1.9 | 24.5 | 5.6 | 4.1 | 29.5 | 6.6 | 4.5 | 15 | 15 | 15 |
| y9 (1+) | 15 | 3 | 1.7 | 20.4 | 6.1 | 3.2 | 25.3 | 6.8 | 3.6 | 15 | 15 | 15 |
| y10 (1+) | 14.5 | 6 | 1.8 | 24.3 | 6.4 | 4.5 | 28.3 | 8.8 | 4.8 | 15 | 15 | 15 |
| sum | 12.4 | 3.2 | 1.1 | 18.5 | 5.4 | 3.5 | 22.3 | 6.3 | 3.7 | 15 | 15 | 15 |
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