Show Nav →
Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

SNCG

Previous Assay Next Assay
Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
SNCG BCSG1, PERSYN, PRSN
Sequence Length (AA) Molecular Weight (Da)
127 13331
Protein Name
Gamma-synuclein
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MDVFKKGFSI AKEGVVGAVE KTKQGVTEAA EKTKEGVMYV GAKTKENVVQ
60 70 80 90 100
SVTSVAEKTK EQANAVSEAV VSSVNTVATK TVEEAENIAV TSGVVRKEDL
110 120 127
RPSAPQQEGE ASKEKEEVAE EAQSGGD

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node
to view detailed assay information below
All other points link out to UniProt


Phosphorylation Acetylation Ubiquitylation Other

loading

Loader

Assay Details for CPTAC-1459 Collapse assay details

Data source: Panorama

Official Gene Symbol
SNCG
Peptide Sequence
EGVVGAVEK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
13
Peptide End
21
CPTAC ID
CPTAC-1459
Peptide Molecular Mass
886.4760
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
depleted digested plasma
Submitting Laboratory
Broad Institute
Submitting Lab PI
Steven A. Carr

Publication

View Details (opens in a new window)

Large-Scale Interlaboratory Study to Develop, Analytically Validate and Apply Highly Multiplexed, Quantitative Peptide Assays to Measure Cancer-Relevant Proteins in Plasma. Abbatiello SE, Schilling B, Mani DR, Zimmerman LJ, Hall SC, MacLean B, Albertolle M, Allen S, Burgess M, Cusack MP, Gosh M, Hedrick V, Held JM, Inerowicz HD, Jackson A, Keshishian H, Kinsinger CR, Lyssand J, Makowski L, Mesri M, Rodriguez H, Rudnick P, Sadowski P, Sedransk N, Shaddox K, Skates SJ, Kuhn E, Smith D, Whiteaker JR, Whitwell C, Zhang S, Borchers CH, Fisher SJ, Gibson BW, Liebler DC, MacCoss MJ, Neubert TA, Paulovich AG, Regnier FE, Tempst P, Carr SA. Mol Cell Proteomics. 2015 Sep;14(9):2357-74. doi: 10.1074/mcp.M114.047050. Epub 2015 Feb 18. PMID: 25693799

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Quantiva TSQ
Internal Standard
heavy stable isotope peptide (delta 8-10)
Peptide Standard Purity
>95%
Peptide Standard Label Type
15N
LC
Easy NanoLC1000
Column Packing
Reprosil C18, 1.9um, 200A
Column Dimensions
0.075 x 150 mm
Flow Rate
200 nL/min

Assay Multiplexing Expand assay panel

Broad Institute-Plasma-based Candidate Cancer Biomarkers

CPTAC-1372:
ALDOC.
ALQASALNAWR 		CPTAC-1373:
ALDOC.
AEVNGLAAQGK 		CPTAC-1374:
ALDOC.
ELSDIALR
CPTAC-1375:
ALDOC.
TPSALAILENANVLAR 		CPTAC-1376:
ALDOC.
QVLFSADDR 		CPTAC-1377:
ANXA1.
ALYEAGER
CPTAC-1378:
ANXA1.
GTDVNVFNTILTTR 		CPTAC-1379:
ANXA1.
AAYLQETGKPLDETLK 		CPTAC-1380:
ANXA1.
TPAQFDADELR
CPTAC-1381:
ANXA4.
VLVSLSAGGR 		CPTAC-1382:
ANXA4.
GLGTDEDAIISVLAYR 		CPTAC-1383:
ANXA4.
DEGNYLDDALVR
CPTAC-1384:
ANXA4.
GAGTDEGC[+57.0]LIEILASR 		CPTAC-1385:
ANXA7.
LYQAGEGR 		CPTAC-1386:
ANXA7.
SEIDLVQIK
CPTAC-1387:
ANXA7.
GAGTDDSTLVR 		CPTAC-1388:
ANXA7.
EFSGYVESGLK 		CPTAC-1389:
ANXA7.
GFGTDEQAIVDVVANR
CPTAC-1390:
CALR.
QIDNPDYK 		CPTAC-1391:
CALR.
FYALSASFEPFSNK 		CPTAC-1392:
CALR.
EQFLDGDGWTSR
CPTAC-1393:
CLIC1.
LHIVQVVC[+57.0]K 		CPTAC-1394:
CLIC1.
YLSNAYAR 		CPTAC-1395:
CLIC1.
IEEFLEAVLC[+57.0]PPR
CPTAC-1396:
CLIC1.
GVTFNVTTVDTK 		CPTAC-1397:
CLIC1.
GFTIPEAFR 		CPTAC-1398:
EZR.
IQVWHAEHR
CPTAC-1399:
EZR.
EDEVEEWQHR 		CPTAC-1400:
EZR.
SGYLSSER 		CPTAC-1401:
EZR.
IALLEEAR
CPTAC-1402:
EZR.
SQEQLAAELAEYTAK 		CPTAC-1403:
FSCN1.
FLIVAHDDGR 		CPTAC-1404:
FSCN1.
YLAPSGPSGTLK
CPTAC-1405:
FSCN1.
VTGTLDANR 		CPTAC-1406:
FSCN1.
LSC[+57.0]FAQTVSPAEK 		CPTAC-1407:
FTL.
LGGPEAGLGEYLFER
CPTAC-1408:
FTL.
KPAEDEWGK 		CPTAC-1409:
FEN1.
SIEEIVR 		CPTAC-1410:
FEN1.
QLQQAQAAGAEQEVEK
CPTAC-1411:
FEN1.
LIADVAPSAIR 		CPTAC-1412:
GSTP1.
FQDGDLTLYQSNTILR 		CPTAC-1413:
GSTP1.
EEVVTVETWQEGSLK
CPTAC-1414:
GSTP1.
PPYTVVYFPVR 		CPTAC-1415:
GSTP1.
ASC[+57.0]LYGQLPK 		CPTAC-1416:
GLO1.
FSLYFLAYEDK
CPTAC-1417:
GLO1.
SLDFYTR 		CPTAC-1418:
GLO1.
IAWALSR 		CPTAC-1419:
GLO1.
GFGHIGIAVPDVYSAC[+57.0]K
CPTAC-1420:
GLO1.
FEELGVK 		CPTAC-1421:
GRB2.
FNSLNELVDYHR 		CPTAC-1422:
GRB2.
FGNDVQHFK
CPTAC-1423:
GRB2.
NYVTPVNR 		CPTAC-1424:
GRB2.
ATADDELSFK 		CPTAC-1425:
GRB2.
ESESAPGDFSLSVK
CPTAC-1426:
HSPB1.
LFDQAFGLPR 		CPTAC-1427:
HSPB1.
AQLGGPEAAK 		CPTAC-1428:
HSPB1.
VSLDVNHFAPDELTVK
CPTAC-1429:
HSPB1.
DGVVEITGK 		CPTAC-1430:
HSPB1.
QLSSGVSEIR 		CPTAC-1431:
IL18.
EDELGDR
CPTAC-1432:
IL18.
SDIIFFQR 		CPTAC-1433:
IL18.
TIFIISMYK 		CPTAC-1434:
IL18.
ISTLSC[+57.0]ENK
CPTAC-1435:
IL18.
GMAVTISVK 		CPTAC-1436:
LGALS1.
DGGAWGTEQR 		CPTAC-1437:
LGALS1.
DSNNLC[+57.0]LHFNPR
CPTAC-1438:
LGALS1.
FNAHGDANTIVC[+57.0]NSK 		CPTAC-1439:
LGALS1.
SFVLNLGK 		CPTAC-1440:
LGALS1.
LPDGYEFK
CPTAC-1441:
PDLIM1.
C[+57.0]GTGIVGVFVK 		CPTAC-1442:
PDLIM1.
VAASIGNAQK 		CPTAC-1443:
PDLIM1.
VWSPLVTEEGK
CPTAC-1444:
PDLIM1.
DFEQPLAISR 		CPTAC-1445:
PRDX2.
GLFIIDGK 		CPTAC-1446:
PRDX2.
TDEGIAYR
CPTAC-1447:
PRDX2.
ATAVVDGAFK 		CPTAC-1448:
PRDX4.
DYGVYLEDSGHTLR 		CPTAC-1449:
PRDX4.
LVQAFQYTDK
CPTAC-1450:
PRDX4.
IPLLSDLTHQISK 		CPTAC-1451:
PRDX4.
QITLNDLPVGR 		CPTAC-1452:
S100A1.
ELLQTELSGFLDAQK
CPTAC-1453:
S100A2.
ELPSFVGEK 		CPTAC-1454:
S100B.
AMVALIDVFHQYSGR 		CPTAC-1455:
S100B.
ELINNELSHFLEEIK
CPTAC-1456:
RAD23B.
EQVIAALR 		CPTAC-1457:
RAD23B.
IDIDPEETVK 		CPTAC-1458:
RAD23B.
ILNDDTALK
CPTAC-1459:
SNCG.
EGVVGAVEK 		CPTAC-1460:
SNCG.
ENVVQSVTSVAEK 		CPTAC-1461:
SNCG.
TVEEAENIAVTSGVVR
CPTAC-1462:
TPM1.
LVIIESDLER 		CPTAC-1463:
TPM1.
SIDDLEDELYAQK 		CPTAC-1464:
TPM1.
QLEDELVSLQK
CPTAC-1465:
UBE2C.
YLQETYSK 		CPTAC-1466:
UBE2C.
LSLEFPSGYPYNAPTVK 		CPTAC-1467:
UBE2C.
DPAATSVAAAR
CPTAC-1468:
UBE2C.
GISAFPESDNLFK 		CPTAC-1469:
UBE2I.
DDYPSSPPK 		CPTAC-1470:
UBE2I.
DWRPAITIK
CPTAC-1471:
UBE2I.
DHPFGFVAVPTK

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (1+) 2.1 1.6 1.1 3.3 6.4 2.8 3.9 6.6 3 15 15 15
y6 (1+) 2.7 2.3 1.1 3.1 6.1 2.6 4.1 6.5 2.8 15 15 15
y4 (1+) 5.8 2 1.8 6.5 6.3 3.3 8.7 6.6 3.8 15 15 15
sum 1.8 1.5 0.9 2.9 6.3 2.6 3.4 6.5 2.8 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments

Assay Details for CPTAC-1460 Collapse assay details

Data source: Panorama

Official Gene Symbol
SNCG
Peptide Sequence
ENVVQSVTSVAEK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
46
Peptide End
58
CPTAC ID
CPTAC-1460
Peptide Molecular Mass
1,388.7147
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
depleted digested plasma
Submitting Laboratory
Broad Institute
Submitting Lab PI
Steven A. Carr

Publication

View Details (opens in a new window)

Large-Scale Interlaboratory Study to Develop, Analytically Validate and Apply Highly Multiplexed, Quantitative Peptide Assays to Measure Cancer-Relevant Proteins in Plasma. Abbatiello SE, Schilling B, Mani DR, Zimmerman LJ, Hall SC, MacLean B, Albertolle M, Allen S, Burgess M, Cusack MP, Gosh M, Hedrick V, Held JM, Inerowicz HD, Jackson A, Keshishian H, Kinsinger CR, Lyssand J, Makowski L, Mesri M, Rodriguez H, Rudnick P, Sadowski P, Sedransk N, Shaddox K, Skates SJ, Kuhn E, Smith D, Whiteaker JR, Whitwell C, Zhang S, Borchers CH, Fisher SJ, Gibson BW, Liebler DC, MacCoss MJ, Neubert TA, Paulovich AG, Regnier FE, Tempst P, Carr SA. Mol Cell Proteomics. 2015 Sep;14(9):2357-74. doi: 10.1074/mcp.M114.047050. Epub 2015 Feb 18. PMID: 25693799

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Quantiva TSQ
Internal Standard
heavy stable isotope peptide (delta 8-10)
Peptide Standard Purity
>95%
Peptide Standard Label Type
15N
LC
Easy NanoLC1000
Column Packing
Reprosil C18, 1.9um, 200A
Column Dimensions
0.075 x 150 mm
Flow Rate
200 nL/min

Assay Multiplexing Expand assay panel

Broad Institute-Plasma-based Candidate Cancer Biomarkers

CPTAC-1372:
ALDOC.
ALQASALNAWR 		CPTAC-1373:
ALDOC.
AEVNGLAAQGK 		CPTAC-1374:
ALDOC.
ELSDIALR
CPTAC-1375:
ALDOC.
TPSALAILENANVLAR 		CPTAC-1376:
ALDOC.
QVLFSADDR 		CPTAC-1377:
ANXA1.
ALYEAGER
CPTAC-1378:
ANXA1.
GTDVNVFNTILTTR 		CPTAC-1379:
ANXA1.
AAYLQETGKPLDETLK 		CPTAC-1380:
ANXA1.
TPAQFDADELR
CPTAC-1381:
ANXA4.
VLVSLSAGGR 		CPTAC-1382:
ANXA4.
GLGTDEDAIISVLAYR 		CPTAC-1383:
ANXA4.
DEGNYLDDALVR
CPTAC-1384:
ANXA4.
GAGTDEGC[+57.0]LIEILASR 		CPTAC-1385:
ANXA7.
LYQAGEGR 		CPTAC-1386:
ANXA7.
SEIDLVQIK
CPTAC-1387:
ANXA7.
GAGTDDSTLVR 		CPTAC-1388:
ANXA7.
EFSGYVESGLK 		CPTAC-1389:
ANXA7.
GFGTDEQAIVDVVANR
CPTAC-1390:
CALR.
QIDNPDYK 		CPTAC-1391:
CALR.
FYALSASFEPFSNK 		CPTAC-1392:
CALR.
EQFLDGDGWTSR
CPTAC-1393:
CLIC1.
LHIVQVVC[+57.0]K 		CPTAC-1394:
CLIC1.
YLSNAYAR 		CPTAC-1395:
CLIC1.
IEEFLEAVLC[+57.0]PPR
CPTAC-1396:
CLIC1.
GVTFNVTTVDTK 		CPTAC-1397:
CLIC1.
GFTIPEAFR 		CPTAC-1398:
EZR.
IQVWHAEHR
CPTAC-1399:
EZR.
EDEVEEWQHR 		CPTAC-1400:
EZR.
SGYLSSER 		CPTAC-1401:
EZR.
IALLEEAR
CPTAC-1402:
EZR.
SQEQLAAELAEYTAK 		CPTAC-1403:
FSCN1.
FLIVAHDDGR 		CPTAC-1404:
FSCN1.
YLAPSGPSGTLK
CPTAC-1405:
FSCN1.
VTGTLDANR 		CPTAC-1406:
FSCN1.
LSC[+57.0]FAQTVSPAEK 		CPTAC-1407:
FTL.
LGGPEAGLGEYLFER
CPTAC-1408:
FTL.
KPAEDEWGK 		CPTAC-1409:
FEN1.
SIEEIVR 		CPTAC-1410:
FEN1.
QLQQAQAAGAEQEVEK
CPTAC-1411:
FEN1.
LIADVAPSAIR 		CPTAC-1412:
GSTP1.
FQDGDLTLYQSNTILR 		CPTAC-1413:
GSTP1.
EEVVTVETWQEGSLK
CPTAC-1414:
GSTP1.
PPYTVVYFPVR 		CPTAC-1415:
GSTP1.
ASC[+57.0]LYGQLPK 		CPTAC-1416:
GLO1.
FSLYFLAYEDK
CPTAC-1417:
GLO1.
SLDFYTR 		CPTAC-1418:
GLO1.
IAWALSR 		CPTAC-1419:
GLO1.
GFGHIGIAVPDVYSAC[+57.0]K
CPTAC-1420:
GLO1.
FEELGVK 		CPTAC-1421:
GRB2.
FNSLNELVDYHR 		CPTAC-1422:
GRB2.
FGNDVQHFK
CPTAC-1423:
GRB2.
NYVTPVNR 		CPTAC-1424:
GRB2.
ATADDELSFK 		CPTAC-1425:
GRB2.
ESESAPGDFSLSVK
CPTAC-1426:
HSPB1.
LFDQAFGLPR 		CPTAC-1427:
HSPB1.
AQLGGPEAAK 		CPTAC-1428:
HSPB1.
VSLDVNHFAPDELTVK
CPTAC-1429:
HSPB1.
DGVVEITGK 		CPTAC-1430:
HSPB1.
QLSSGVSEIR 		CPTAC-1431:
IL18.
EDELGDR
CPTAC-1432:
IL18.
SDIIFFQR 		CPTAC-1433:
IL18.
TIFIISMYK 		CPTAC-1434:
IL18.
ISTLSC[+57.0]ENK
CPTAC-1435:
IL18.
GMAVTISVK 		CPTAC-1436:
LGALS1.
DGGAWGTEQR 		CPTAC-1437:
LGALS1.
DSNNLC[+57.0]LHFNPR
CPTAC-1438:
LGALS1.
FNAHGDANTIVC[+57.0]NSK 		CPTAC-1439:
LGALS1.
SFVLNLGK 		CPTAC-1440:
LGALS1.
LPDGYEFK
CPTAC-1441:
PDLIM1.
C[+57.0]GTGIVGVFVK 		CPTAC-1442:
PDLIM1.
VAASIGNAQK 		CPTAC-1443:
PDLIM1.
VWSPLVTEEGK
CPTAC-1444:
PDLIM1.
DFEQPLAISR 		CPTAC-1445:
PRDX2.
GLFIIDGK 		CPTAC-1446:
PRDX2.
TDEGIAYR
CPTAC-1447:
PRDX2.
ATAVVDGAFK 		CPTAC-1448:
PRDX4.
DYGVYLEDSGHTLR 		CPTAC-1449:
PRDX4.
LVQAFQYTDK
CPTAC-1450:
PRDX4.
IPLLSDLTHQISK 		CPTAC-1451:
PRDX4.
QITLNDLPVGR 		CPTAC-1452:
S100A1.
ELLQTELSGFLDAQK
CPTAC-1453:
S100A2.
ELPSFVGEK 		CPTAC-1454:
S100B.
AMVALIDVFHQYSGR 		CPTAC-1455:
S100B.
ELINNELSHFLEEIK
CPTAC-1456:
RAD23B.
EQVIAALR 		CPTAC-1457:
RAD23B.
IDIDPEETVK 		CPTAC-1458:
RAD23B.
ILNDDTALK
CPTAC-1459:
SNCG.
EGVVGAVEK 		CPTAC-1460:
SNCG.
ENVVQSVTSVAEK 		CPTAC-1461:
SNCG.
TVEEAENIAVTSGVVR
CPTAC-1462:
TPM1.
LVIIESDLER 		CPTAC-1463:
TPM1.
SIDDLEDELYAQK 		CPTAC-1464:
TPM1.
QLEDELVSLQK
CPTAC-1465:
UBE2C.
YLQETYSK 		CPTAC-1466:
UBE2C.
LSLEFPSGYPYNAPTVK 		CPTAC-1467:
UBE2C.
DPAATSVAAAR
CPTAC-1468:
UBE2C.
GISAFPESDNLFK 		CPTAC-1469:
UBE2I.
DDYPSSPPK 		CPTAC-1470:
UBE2I.
DWRPAITIK
CPTAC-1471:
UBE2I.
DHPFGFVAVPTK

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y10 (1+) 6.9 2.8 2.2 8.7 6.1 4.3 11.1 6.7 4.8 15 15 15
y8 (1+) 3.8 2.5 2.1 5.5 5.4 3.1 6.7 6 3.7 15 15 15
y9 (1+) 4.8 2.3 2.1 6.2 6.3 3.2 7.8 6.7 3.8 15 15 15
sum 2.4 2 1.5 5.4 5.7 3 5.9 6 3.4 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments

Assay Details for CPTAC-1461 Collapse assay details

Data source: Panorama

Official Gene Symbol
SNCG
Peptide Sequence
TVEEAENIAVTSGVVR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
81
Peptide End
96
CPTAC ID
CPTAC-1461
Peptide Molecular Mass
1,672.8632
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
depleted digested plasma
Submitting Laboratory
Broad Institute
Submitting Lab PI
Steven A. Carr

Publication

View Details (opens in a new window)

Large-Scale Interlaboratory Study to Develop, Analytically Validate and Apply Highly Multiplexed, Quantitative Peptide Assays to Measure Cancer-Relevant Proteins in Plasma. Abbatiello SE, Schilling B, Mani DR, Zimmerman LJ, Hall SC, MacLean B, Albertolle M, Allen S, Burgess M, Cusack MP, Gosh M, Hedrick V, Held JM, Inerowicz HD, Jackson A, Keshishian H, Kinsinger CR, Lyssand J, Makowski L, Mesri M, Rodriguez H, Rudnick P, Sadowski P, Sedransk N, Shaddox K, Skates SJ, Kuhn E, Smith D, Whiteaker JR, Whitwell C, Zhang S, Borchers CH, Fisher SJ, Gibson BW, Liebler DC, MacCoss MJ, Neubert TA, Paulovich AG, Regnier FE, Tempst P, Carr SA. Mol Cell Proteomics. 2015 Sep;14(9):2357-74. doi: 10.1074/mcp.M114.047050. Epub 2015 Feb 18. PMID: 25693799

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Quantiva TSQ
Internal Standard
heavy stable isotope peptide (delta 8-10)
Peptide Standard Purity
>95%
Peptide Standard Label Type
15N
LC
Easy NanoLC1000
Column Packing
Reprosil C18, 1.9um, 200A
Column Dimensions
0.075 x 150 mm
Flow Rate
200 nL/min

Assay Multiplexing Expand assay panel

Broad Institute-Plasma-based Candidate Cancer Biomarkers

CPTAC-1372:
ALDOC.
ALQASALNAWR 		CPTAC-1373:
ALDOC.
AEVNGLAAQGK 		CPTAC-1374:
ALDOC.
ELSDIALR
CPTAC-1375:
ALDOC.
TPSALAILENANVLAR 		CPTAC-1376:
ALDOC.
QVLFSADDR 		CPTAC-1377:
ANXA1.
ALYEAGER
CPTAC-1378:
ANXA1.
GTDVNVFNTILTTR 		CPTAC-1379:
ANXA1.
AAYLQETGKPLDETLK 		CPTAC-1380:
ANXA1.
TPAQFDADELR
CPTAC-1381:
ANXA4.
VLVSLSAGGR 		CPTAC-1382:
ANXA4.
GLGTDEDAIISVLAYR 		CPTAC-1383:
ANXA4.
DEGNYLDDALVR
CPTAC-1384:
ANXA4.
GAGTDEGC[+57.0]LIEILASR 		CPTAC-1385:
ANXA7.
LYQAGEGR 		CPTAC-1386:
ANXA7.
SEIDLVQIK
CPTAC-1387:
ANXA7.
GAGTDDSTLVR 		CPTAC-1388:
ANXA7.
EFSGYVESGLK 		CPTAC-1389:
ANXA7.
GFGTDEQAIVDVVANR
CPTAC-1390:
CALR.
QIDNPDYK 		CPTAC-1391:
CALR.
FYALSASFEPFSNK 		CPTAC-1392:
CALR.
EQFLDGDGWTSR
CPTAC-1393:
CLIC1.
LHIVQVVC[+57.0]K 		CPTAC-1394:
CLIC1.
YLSNAYAR 		CPTAC-1395:
CLIC1.
IEEFLEAVLC[+57.0]PPR
CPTAC-1396:
CLIC1.
GVTFNVTTVDTK 		CPTAC-1397:
CLIC1.
GFTIPEAFR 		CPTAC-1398:
EZR.
IQVWHAEHR
CPTAC-1399:
EZR.
EDEVEEWQHR 		CPTAC-1400:
EZR.
SGYLSSER 		CPTAC-1401:
EZR.
IALLEEAR
CPTAC-1402:
EZR.
SQEQLAAELAEYTAK 		CPTAC-1403:
FSCN1.
FLIVAHDDGR 		CPTAC-1404:
FSCN1.
YLAPSGPSGTLK
CPTAC-1405:
FSCN1.
VTGTLDANR 		CPTAC-1406:
FSCN1.
LSC[+57.0]FAQTVSPAEK 		CPTAC-1407:
FTL.
LGGPEAGLGEYLFER
CPTAC-1408:
FTL.
KPAEDEWGK 		CPTAC-1409:
FEN1.
SIEEIVR 		CPTAC-1410:
FEN1.
QLQQAQAAGAEQEVEK
CPTAC-1411:
FEN1.
LIADVAPSAIR 		CPTAC-1412:
GSTP1.
FQDGDLTLYQSNTILR 		CPTAC-1413:
GSTP1.
EEVVTVETWQEGSLK
CPTAC-1414:
GSTP1.
PPYTVVYFPVR 		CPTAC-1415:
GSTP1.
ASC[+57.0]LYGQLPK 		CPTAC-1416:
GLO1.
FSLYFLAYEDK
CPTAC-1417:
GLO1.
SLDFYTR 		CPTAC-1418:
GLO1.
IAWALSR 		CPTAC-1419:
GLO1.
GFGHIGIAVPDVYSAC[+57.0]K
CPTAC-1420:
GLO1.
FEELGVK 		CPTAC-1421:
GRB2.
FNSLNELVDYHR 		CPTAC-1422:
GRB2.
FGNDVQHFK
CPTAC-1423:
GRB2.
NYVTPVNR 		CPTAC-1424:
GRB2.
ATADDELSFK 		CPTAC-1425:
GRB2.
ESESAPGDFSLSVK
CPTAC-1426:
HSPB1.
LFDQAFGLPR 		CPTAC-1427:
HSPB1.
AQLGGPEAAK 		CPTAC-1428:
HSPB1.
VSLDVNHFAPDELTVK
CPTAC-1429:
HSPB1.
DGVVEITGK 		CPTAC-1430:
HSPB1.
QLSSGVSEIR 		CPTAC-1431:
IL18.
EDELGDR
CPTAC-1432:
IL18.
SDIIFFQR 		CPTAC-1433:
IL18.
TIFIISMYK 		CPTAC-1434:
IL18.
ISTLSC[+57.0]ENK
CPTAC-1435:
IL18.
GMAVTISVK 		CPTAC-1436:
LGALS1.
DGGAWGTEQR 		CPTAC-1437:
LGALS1.
DSNNLC[+57.0]LHFNPR
CPTAC-1438:
LGALS1.
FNAHGDANTIVC[+57.0]NSK 		CPTAC-1439:
LGALS1.
SFVLNLGK 		CPTAC-1440:
LGALS1.
LPDGYEFK
CPTAC-1441:
PDLIM1.
C[+57.0]GTGIVGVFVK 		CPTAC-1442:
PDLIM1.
VAASIGNAQK 		CPTAC-1443:
PDLIM1.
VWSPLVTEEGK
CPTAC-1444:
PDLIM1.
DFEQPLAISR 		CPTAC-1445:
PRDX2.
GLFIIDGK 		CPTAC-1446:
PRDX2.
TDEGIAYR
CPTAC-1447:
PRDX2.
ATAVVDGAFK 		CPTAC-1448:
PRDX4.
DYGVYLEDSGHTLR 		CPTAC-1449:
PRDX4.
LVQAFQYTDK
CPTAC-1450:
PRDX4.
IPLLSDLTHQISK 		CPTAC-1451:
PRDX4.
QITLNDLPVGR 		CPTAC-1452:
S100A1.
ELLQTELSGFLDAQK
CPTAC-1453:
S100A2.
ELPSFVGEK 		CPTAC-1454:
S100B.
AMVALIDVFHQYSGR 		CPTAC-1455:
S100B.
ELINNELSHFLEEIK
CPTAC-1456:
RAD23B.
EQVIAALR 		CPTAC-1457:
RAD23B.
IDIDPEETVK 		CPTAC-1458:
RAD23B.
ILNDDTALK
CPTAC-1459:
SNCG.
EGVVGAVEK 		CPTAC-1460:
SNCG.
ENVVQSVTSVAEK 		CPTAC-1461:
SNCG.
TVEEAENIAVTSGVVR
CPTAC-1462:
TPM1.
LVIIESDLER 		CPTAC-1463:
TPM1.
SIDDLEDELYAQK 		CPTAC-1464:
TPM1.
QLEDELVSLQK
CPTAC-1465:
UBE2C.
YLQETYSK 		CPTAC-1466:
UBE2C.
LSLEFPSGYPYNAPTVK 		CPTAC-1467:
UBE2C.
DPAATSVAAAR
CPTAC-1468:
UBE2C.
GISAFPESDNLFK 		CPTAC-1469:
UBE2I.
DDYPSSPPK 		CPTAC-1470:
UBE2I.
DWRPAITIK
CPTAC-1471:
UBE2I.
DHPFGFVAVPTK

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 13.7 6.5 6.5 14.3 9.4 6 19.8 11.4 8.8 15 15 15
y5 (1+) 17.5 5.5 5.2 26.4 8.5 4.9 31.7 10.1 7.1 15 15 15
y7 (1+) 14 7.7 5.9 17.5 13.5 6.6 22.4 15.5 8.9 15 15 15
sum 9.2 4.1 4.2 11.2 8.5 4.7 14.5 9.4 6.3 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments

Assay Details for CPTAC-133 Collapse assay details

Data source: Panorama

Official Gene Symbol
SNCG
Peptide Sequence
TVEEAENIAVTSGVVR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
81
Peptide End
96
CPTAC ID
CPTAC-133
Peptide Molecular Mass
1,672.8632
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Assay Multiplexing Expand assay panel

Broad Institute-Plasma-based Candidate Cancer Biomarkers

CPTAC-1372:
ALDOC.
ALQASALNAWR 		CPTAC-1373:
ALDOC.
AEVNGLAAQGK 		CPTAC-1374:
ALDOC.
ELSDIALR
CPTAC-1375:
ALDOC.
TPSALAILENANVLAR 		CPTAC-1376:
ALDOC.
QVLFSADDR 		CPTAC-1377:
ANXA1.
ALYEAGER
CPTAC-1378:
ANXA1.
GTDVNVFNTILTTR 		CPTAC-1379:
ANXA1.
AAYLQETGKPLDETLK 		CPTAC-1380:
ANXA1.
TPAQFDADELR
CPTAC-1381:
ANXA4.
VLVSLSAGGR 		CPTAC-1382:
ANXA4.
GLGTDEDAIISVLAYR 		CPTAC-1383:
ANXA4.
DEGNYLDDALVR
CPTAC-1384:
ANXA4.
GAGTDEGC[+57.0]LIEILASR 		CPTAC-1385:
ANXA7.
LYQAGEGR 		CPTAC-1386:
ANXA7.
SEIDLVQIK
CPTAC-1387:
ANXA7.
GAGTDDSTLVR 		CPTAC-1388:
ANXA7.
EFSGYVESGLK 		CPTAC-1389:
ANXA7.
GFGTDEQAIVDVVANR
CPTAC-1390:
CALR.
QIDNPDYK 		CPTAC-1391:
CALR.
FYALSASFEPFSNK 		CPTAC-1392:
CALR.
EQFLDGDGWTSR
CPTAC-1393:
CLIC1.
LHIVQVVC[+57.0]K 		CPTAC-1394:
CLIC1.
YLSNAYAR 		CPTAC-1395:
CLIC1.
IEEFLEAVLC[+57.0]PPR
CPTAC-1396:
CLIC1.
GVTFNVTTVDTK 		CPTAC-1397:
CLIC1.
GFTIPEAFR 		CPTAC-1398:
EZR.
IQVWHAEHR
CPTAC-1399:
EZR.
EDEVEEWQHR 		CPTAC-1400:
EZR.
SGYLSSER 		CPTAC-1401:
EZR.
IALLEEAR
CPTAC-1402:
EZR.
SQEQLAAELAEYTAK 		CPTAC-1403:
FSCN1.
FLIVAHDDGR 		CPTAC-1404:
FSCN1.
YLAPSGPSGTLK
CPTAC-1405:
FSCN1.
VTGTLDANR 		CPTAC-1406:
FSCN1.
LSC[+57.0]FAQTVSPAEK 		CPTAC-1407:
FTL.
LGGPEAGLGEYLFER
CPTAC-1408:
FTL.
KPAEDEWGK 		CPTAC-1409:
FEN1.
SIEEIVR 		CPTAC-1410:
FEN1.
QLQQAQAAGAEQEVEK
CPTAC-1411:
FEN1.
LIADVAPSAIR 		CPTAC-1412:
GSTP1.
FQDGDLTLYQSNTILR 		CPTAC-1413:
GSTP1.
EEVVTVETWQEGSLK
CPTAC-1414:
GSTP1.
PPYTVVYFPVR 		CPTAC-1415:
GSTP1.
ASC[+57.0]LYGQLPK 		CPTAC-1416:
GLO1.
FSLYFLAYEDK
CPTAC-1417:
GLO1.
SLDFYTR 		CPTAC-1418:
GLO1.
IAWALSR 		CPTAC-1419:
GLO1.
GFGHIGIAVPDVYSAC[+57.0]K
CPTAC-1420:
GLO1.
FEELGVK 		CPTAC-1421:
GRB2.
FNSLNELVDYHR 		CPTAC-1422:
GRB2.
FGNDVQHFK
CPTAC-1423:
GRB2.
NYVTPVNR 		CPTAC-1424:
GRB2.
ATADDELSFK 		CPTAC-1425:
GRB2.
ESESAPGDFSLSVK
CPTAC-1426:
HSPB1.
LFDQAFGLPR 		CPTAC-1427:
HSPB1.
AQLGGPEAAK 		CPTAC-1428:
HSPB1.
VSLDVNHFAPDELTVK
CPTAC-1429:
HSPB1.
DGVVEITGK 		CPTAC-1430:
HSPB1.
QLSSGVSEIR 		CPTAC-1431:
IL18.
EDELGDR
CPTAC-1432:
IL18.
SDIIFFQR 		CPTAC-1433:
IL18.
TIFIISMYK 		CPTAC-1434:
IL18.
ISTLSC[+57.0]ENK
CPTAC-1435:
IL18.
GMAVTISVK 		CPTAC-1436:
LGALS1.
DGGAWGTEQR 		CPTAC-1437:
LGALS1.
DSNNLC[+57.0]LHFNPR
CPTAC-1438:
LGALS1.
FNAHGDANTIVC[+57.0]NSK 		CPTAC-1439:
LGALS1.
SFVLNLGK 		CPTAC-1440:
LGALS1.
LPDGYEFK
CPTAC-1441:
PDLIM1.
C[+57.0]GTGIVGVFVK 		CPTAC-1442:
PDLIM1.
VAASIGNAQK 		CPTAC-1443:
PDLIM1.
VWSPLVTEEGK
CPTAC-1444:
PDLIM1.
DFEQPLAISR 		CPTAC-1445:
PRDX2.
GLFIIDGK 		CPTAC-1446:
PRDX2.
TDEGIAYR
CPTAC-1447:
PRDX2.
ATAVVDGAFK 		CPTAC-1448:
PRDX4.
DYGVYLEDSGHTLR 		CPTAC-1449:
PRDX4.
LVQAFQYTDK
CPTAC-1450:
PRDX4.
IPLLSDLTHQISK 		CPTAC-1451:
PRDX4.
QITLNDLPVGR 		CPTAC-1452:
S100A1.
ELLQTELSGFLDAQK
CPTAC-1453:
S100A2.
ELPSFVGEK 		CPTAC-1454:
S100B.
AMVALIDVFHQYSGR 		CPTAC-1455:
S100B.
ELINNELSHFLEEIK
CPTAC-1456:
RAD23B.
EQVIAALR 		CPTAC-1457:
RAD23B.
IDIDPEETVK 		CPTAC-1458:
RAD23B.
ILNDDTALK
CPTAC-1459:
SNCG.
EGVVGAVEK 		CPTAC-1460:
SNCG.
ENVVQSVTSVAEK 		CPTAC-1461:
SNCG.
TVEEAENIAVTSGVVR
CPTAC-1462:
TPM1.
LVIIESDLER 		CPTAC-1463:
TPM1.
SIDDLEDELYAQK 		CPTAC-1464:
TPM1.
QLEDELVSLQK
CPTAC-1465:
UBE2C.
YLQETYSK 		CPTAC-1466:
UBE2C.
LSLEFPSGYPYNAPTVK 		CPTAC-1467:
UBE2C.
DPAATSVAAAR
CPTAC-1468:
UBE2C.
GISAFPESDNLFK 		CPTAC-1469:
UBE2I.
DDYPSSPPK 		CPTAC-1470:
UBE2I.
DWRPAITIK
CPTAC-1471:
UBE2I.
DHPFGFVAVPTK

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y10 (1+) 11.4 9.9 9 19.5 11.6 11 22.6 15.3 14.2 15 25 15
y8 (1+) 10 7 6.2 16.6 7.2 12.2 19.4 10 13.7 15 25 15
y6 (1+) 8.4 6.9 6.8 11 7.8 12.7 13.8 10.4 14.4 15 25 15
sum 6.9 6.7 4.8 13.7 7.3 11.2 15.3 9.9 12.2 15 25 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments

Assay Details for CPTAC-708 Collapse assay details

Data source: Panorama

Official Gene Symbol
SNCG
Peptide Sequence
TVEEAENIAVTSGVVR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
81
Peptide End
96
CPTAC ID
CPTAC-708
Peptide Molecular Mass
1,672.8632
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
N/A
Matrix
plasma
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Anti-peptide monoclonal antibodies generated for immuno-multiple reaction monitoring-mass spectrometry assays have a high probability of supporting Western blot and ELISA. Schoenherr RM, Saul RG, Whiteaker JR, Yan P, Whiteley GR, Paulovich AG. Mol Cell Proteomics. 2015 Feb;14(2):382-98. doi: 10.1074/mcp.O114.043133. Epub 2014 Dec 15. PMID: 25512614

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 Qtrap
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
15N
LC
Eksigent NanoLC 2D
Column Packing
ReproSil-Pur C18-AQ 3 um
Column Dimensions
100 mm x 75 um ID
Flow Rate
300 nL/min

Assay Multiplexing Expand assay panel

Broad Institute-Plasma-based Candidate Cancer Biomarkers

CPTAC-1372:
ALDOC.
ALQASALNAWR 		CPTAC-1373:
ALDOC.
AEVNGLAAQGK 		CPTAC-1374:
ALDOC.
ELSDIALR
CPTAC-1375:
ALDOC.
TPSALAILENANVLAR 		CPTAC-1376:
ALDOC.
QVLFSADDR 		CPTAC-1377:
ANXA1.
ALYEAGER
CPTAC-1378:
ANXA1.
GTDVNVFNTILTTR 		CPTAC-1379:
ANXA1.
AAYLQETGKPLDETLK 		CPTAC-1380:
ANXA1.
TPAQFDADELR
CPTAC-1381:
ANXA4.
VLVSLSAGGR 		CPTAC-1382:
ANXA4.
GLGTDEDAIISVLAYR 		CPTAC-1383:
ANXA4.
DEGNYLDDALVR
CPTAC-1384:
ANXA4.
GAGTDEGC[+57.0]LIEILASR 		CPTAC-1385:
ANXA7.
LYQAGEGR 		CPTAC-1386:
ANXA7.
SEIDLVQIK
CPTAC-1387:
ANXA7.
GAGTDDSTLVR 		CPTAC-1388:
ANXA7.
EFSGYVESGLK 		CPTAC-1389:
ANXA7.
GFGTDEQAIVDVVANR
CPTAC-1390:
CALR.
QIDNPDYK 		CPTAC-1391:
CALR.
FYALSASFEPFSNK 		CPTAC-1392:
CALR.
EQFLDGDGWTSR
CPTAC-1393:
CLIC1.
LHIVQVVC[+57.0]K 		CPTAC-1394:
CLIC1.
YLSNAYAR 		CPTAC-1395:
CLIC1.
IEEFLEAVLC[+57.0]PPR
CPTAC-1396:
CLIC1.
GVTFNVTTVDTK 		CPTAC-1397:
CLIC1.
GFTIPEAFR 		CPTAC-1398:
EZR.
IQVWHAEHR
CPTAC-1399:
EZR.
EDEVEEWQHR 		CPTAC-1400:
EZR.
SGYLSSER 		CPTAC-1401:
EZR.
IALLEEAR
CPTAC-1402:
EZR.
SQEQLAAELAEYTAK 		CPTAC-1403:
FSCN1.
FLIVAHDDGR 		CPTAC-1404:
FSCN1.
YLAPSGPSGTLK
CPTAC-1405:
FSCN1.
VTGTLDANR 		CPTAC-1406:
FSCN1.
LSC[+57.0]FAQTVSPAEK 		CPTAC-1407:
FTL.
LGGPEAGLGEYLFER
CPTAC-1408:
FTL.
KPAEDEWGK 		CPTAC-1409:
FEN1.
SIEEIVR 		CPTAC-1410:
FEN1.
QLQQAQAAGAEQEVEK
CPTAC-1411:
FEN1.
LIADVAPSAIR 		CPTAC-1412:
GSTP1.
FQDGDLTLYQSNTILR 		CPTAC-1413:
GSTP1.
EEVVTVETWQEGSLK
CPTAC-1414:
GSTP1.
PPYTVVYFPVR 		CPTAC-1415:
GSTP1.
ASC[+57.0]LYGQLPK 		CPTAC-1416:
GLO1.
FSLYFLAYEDK
CPTAC-1417:
GLO1.
SLDFYTR 		CPTAC-1418:
GLO1.
IAWALSR 		CPTAC-1419:
GLO1.
GFGHIGIAVPDVYSAC[+57.0]K
CPTAC-1420:
GLO1.
FEELGVK 		CPTAC-1421:
GRB2.
FNSLNELVDYHR 		CPTAC-1422:
GRB2.
FGNDVQHFK
CPTAC-1423:
GRB2.
NYVTPVNR 		CPTAC-1424:
GRB2.
ATADDELSFK 		CPTAC-1425:
GRB2.
ESESAPGDFSLSVK
CPTAC-1426:
HSPB1.
LFDQAFGLPR 		CPTAC-1427:
HSPB1.
AQLGGPEAAK 		CPTAC-1428:
HSPB1.
VSLDVNHFAPDELTVK
CPTAC-1429:
HSPB1.
DGVVEITGK 		CPTAC-1430:
HSPB1.
QLSSGVSEIR 		CPTAC-1431:
IL18.
EDELGDR
CPTAC-1432:
IL18.
SDIIFFQR 		CPTAC-1433:
IL18.
TIFIISMYK 		CPTAC-1434:
IL18.
ISTLSC[+57.0]ENK
CPTAC-1435:
IL18.
GMAVTISVK 		CPTAC-1436:
LGALS1.
DGGAWGTEQR 		CPTAC-1437:
LGALS1.
DSNNLC[+57.0]LHFNPR
CPTAC-1438:
LGALS1.
FNAHGDANTIVC[+57.0]NSK 		CPTAC-1439:
LGALS1.
SFVLNLGK 		CPTAC-1440:
LGALS1.
LPDGYEFK
CPTAC-1441:
PDLIM1.
C[+57.0]GTGIVGVFVK 		CPTAC-1442:
PDLIM1.
VAASIGNAQK 		CPTAC-1443:
PDLIM1.
VWSPLVTEEGK
CPTAC-1444:
PDLIM1.
DFEQPLAISR 		CPTAC-1445:
PRDX2.
GLFIIDGK 		CPTAC-1446:
PRDX2.
TDEGIAYR
CPTAC-1447:
PRDX2.
ATAVVDGAFK 		CPTAC-1448:
PRDX4.
DYGVYLEDSGHTLR 		CPTAC-1449:
PRDX4.
LVQAFQYTDK
CPTAC-1450:
PRDX4.
IPLLSDLTHQISK 		CPTAC-1451:
PRDX4.
QITLNDLPVGR 		CPTAC-1452:
S100A1.
ELLQTELSGFLDAQK
CPTAC-1453:
S100A2.
ELPSFVGEK 		CPTAC-1454:
S100B.
AMVALIDVFHQYSGR 		CPTAC-1455:
S100B.
ELINNELSHFLEEIK
CPTAC-1456:
RAD23B.
EQVIAALR 		CPTAC-1457:
RAD23B.
IDIDPEETVK 		CPTAC-1458:
RAD23B.
ILNDDTALK
CPTAC-1459:
SNCG.
EGVVGAVEK 		CPTAC-1460:
SNCG.
ENVVQSVTSVAEK 		CPTAC-1461:
SNCG.
TVEEAENIAVTSGVVR
CPTAC-1462:
TPM1.
LVIIESDLER 		CPTAC-1463:
TPM1.
SIDDLEDELYAQK 		CPTAC-1464:
TPM1.
QLEDELVSLQK
CPTAC-1465:
UBE2C.
YLQETYSK 		CPTAC-1466:
UBE2C.
LSLEFPSGYPYNAPTVK 		CPTAC-1467:
UBE2C.
DPAATSVAAAR
CPTAC-1468:
UBE2C.
GISAFPESDNLFK 		CPTAC-1469:
UBE2I.
DDYPSSPPK 		CPTAC-1470:
UBE2I.
DWRPAITIK
CPTAC-1471:
UBE2I.
DHPFGFVAVPTK

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 5.2 3.3 1.6 7.4 3.3 5.4 9 4.7 5.6 15 15 15
y7 (1+) 6.7 3.9 2.5 8.9 4.3 5.7 11.1 5.8 6.2 15 15 15
y8 (1+) 4.8 2.4 4.7 8.6 3.3 7.9 9.8 4.1 9.2 15 15 15
sum 3.3 2.5 2.8 7 3 6.1 7.7 3.9 6.7 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
View in CPTC Antibody Portal
Comments