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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

TPM1

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
TPM1 C15orf13, TMSA
Sequence Length (AA) Molecular Weight (Da)
284 32709
Protein Name
Tropomyosin alpha-1 chain
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MDAIKKKMQM LKLDKENALD RAEQAEADKK AAEDRSKQLE DELVSLQKKL
60 70 80 90 100
KGTEDELDKY SEALKDAQEK LELAEKKATD AEADVASLNR RIQLVEEELD
110 120 130 140 150
RAQERLATAL QKLEEAEKAA DESERGMKVI ESRAQKDEEK MEIQEIQLKE
160 170 180 190 200
AKHIAEDADR KYEEVARKLV IIESDLERAE ERAELSEGKC AELEEELKTV
210 220 230 240 250
TNNLKSLEAQ AEKYSQKEDR YEEEIKVLSD KLKEAETRAE FAERSVTKLE
260 270 280 284
KSIDDLEDEL YAQKLKYKAI SEELDHALND MTSI

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node
to view detailed assay information below
All other points link out to UniProt


Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1462 Collapse assay details

Data source: Panorama

Official Gene Symbol
TPM1
Peptide Sequence
LVIIESDLER
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
169
Peptide End
178
CPTAC ID
CPTAC-1462
Peptide Molecular Mass
1,185.6605
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
depleted digested plasma
Submitting Laboratory
Broad Institute
Submitting Lab PI
Steven A. Carr

Publication

View Details (opens in a new window)

Large-Scale Interlaboratory Study to Develop, Analytically Validate and Apply Highly Multiplexed, Quantitative Peptide Assays to Measure Cancer-Relevant Proteins in Plasma. Abbatiello SE, Schilling B, Mani DR, Zimmerman LJ, Hall SC, MacLean B, Albertolle M, Allen S, Burgess M, Cusack MP, Gosh M, Hedrick V, Held JM, Inerowicz HD, Jackson A, Keshishian H, Kinsinger CR, Lyssand J, Makowski L, Mesri M, Rodriguez H, Rudnick P, Sadowski P, Sedransk N, Shaddox K, Skates SJ, Kuhn E, Smith D, Whiteaker JR, Whitwell C, Zhang S, Borchers CH, Fisher SJ, Gibson BW, Liebler DC, MacCoss MJ, Neubert TA, Paulovich AG, Regnier FE, Tempst P, Carr SA. Mol Cell Proteomics. 2015 Sep;14(9):2357-74. doi: 10.1074/mcp.M114.047050. Epub 2015 Feb 18. PMID: 25693799

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Quantiva TSQ
Internal Standard
heavy stable isotope peptide (delta 8-10)
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Easy NanoLC1000
Column Packing
Reprosil C18, 1.9um, 200A
Column Dimensions
0.075 x 150 mm
Flow Rate
200 nL/min

Assay Multiplexing Expand assay panel

Broad Institute-Plasma-based Candidate Cancer Biomarkers

CPTAC-1372:
ALDOC.
ALQASALNAWR 		CPTAC-1373:
ALDOC.
AEVNGLAAQGK 		CPTAC-1374:
ALDOC.
ELSDIALR
CPTAC-1375:
ALDOC.
TPSALAILENANVLAR 		CPTAC-1376:
ALDOC.
QVLFSADDR 		CPTAC-1377:
ANXA1.
ALYEAGER
CPTAC-1378:
ANXA1.
GTDVNVFNTILTTR 		CPTAC-1379:
ANXA1.
AAYLQETGKPLDETLK 		CPTAC-1380:
ANXA1.
TPAQFDADELR
CPTAC-1381:
ANXA4.
VLVSLSAGGR 		CPTAC-1382:
ANXA4.
GLGTDEDAIISVLAYR 		CPTAC-1383:
ANXA4.
DEGNYLDDALVR
CPTAC-1384:
ANXA4.
GAGTDEGC[+57.0]LIEILASR 		CPTAC-1385:
ANXA7.
LYQAGEGR 		CPTAC-1386:
ANXA7.
SEIDLVQIK
CPTAC-1387:
ANXA7.
GAGTDDSTLVR 		CPTAC-1388:
ANXA7.
EFSGYVESGLK 		CPTAC-1389:
ANXA7.
GFGTDEQAIVDVVANR
CPTAC-1390:
CALR.
QIDNPDYK 		CPTAC-1391:
CALR.
FYALSASFEPFSNK 		CPTAC-1392:
CALR.
EQFLDGDGWTSR
CPTAC-1393:
CLIC1.
LHIVQVVC[+57.0]K 		CPTAC-1394:
CLIC1.
YLSNAYAR 		CPTAC-1395:
CLIC1.
IEEFLEAVLC[+57.0]PPR
CPTAC-1396:
CLIC1.
GVTFNVTTVDTK 		CPTAC-1397:
CLIC1.
GFTIPEAFR 		CPTAC-1398:
EZR.
IQVWHAEHR
CPTAC-1399:
EZR.
EDEVEEWQHR 		CPTAC-1400:
EZR.
SGYLSSER 		CPTAC-1401:
EZR.
IALLEEAR
CPTAC-1402:
EZR.
SQEQLAAELAEYTAK 		CPTAC-1403:
FSCN1.
FLIVAHDDGR 		CPTAC-1404:
FSCN1.
YLAPSGPSGTLK
CPTAC-1405:
FSCN1.
VTGTLDANR 		CPTAC-1406:
FSCN1.
LSC[+57.0]FAQTVSPAEK 		CPTAC-1407:
FTL.
LGGPEAGLGEYLFER
CPTAC-1408:
FTL.
KPAEDEWGK 		CPTAC-1409:
FEN1.
SIEEIVR 		CPTAC-1410:
FEN1.
QLQQAQAAGAEQEVEK
CPTAC-1411:
FEN1.
LIADVAPSAIR 		CPTAC-1412:
GSTP1.
FQDGDLTLYQSNTILR 		CPTAC-1413:
GSTP1.
EEVVTVETWQEGSLK
CPTAC-1414:
GSTP1.
PPYTVVYFPVR 		CPTAC-1415:
GSTP1.
ASC[+57.0]LYGQLPK 		CPTAC-1416:
GLO1.
FSLYFLAYEDK
CPTAC-1417:
GLO1.
SLDFYTR 		CPTAC-1418:
GLO1.
IAWALSR 		CPTAC-1419:
GLO1.
GFGHIGIAVPDVYSAC[+57.0]K
CPTAC-1420:
GLO1.
FEELGVK 		CPTAC-1421:
GRB2.
FNSLNELVDYHR 		CPTAC-1422:
GRB2.
FGNDVQHFK
CPTAC-1423:
GRB2.
NYVTPVNR 		CPTAC-1424:
GRB2.
ATADDELSFK 		CPTAC-1425:
GRB2.
ESESAPGDFSLSVK
CPTAC-1426:
HSPB1.
LFDQAFGLPR 		CPTAC-1427:
HSPB1.
AQLGGPEAAK 		CPTAC-1428:
HSPB1.
VSLDVNHFAPDELTVK
CPTAC-1429:
HSPB1.
DGVVEITGK 		CPTAC-1430:
HSPB1.
QLSSGVSEIR 		CPTAC-1431:
IL18.
EDELGDR
CPTAC-1432:
IL18.
SDIIFFQR 		CPTAC-1433:
IL18.
TIFIISMYK 		CPTAC-1434:
IL18.
ISTLSC[+57.0]ENK
CPTAC-1435:
IL18.
GMAVTISVK 		CPTAC-1436:
LGALS1.
DGGAWGTEQR 		CPTAC-1437:
LGALS1.
DSNNLC[+57.0]LHFNPR
CPTAC-1438:
LGALS1.
FNAHGDANTIVC[+57.0]NSK 		CPTAC-1439:
LGALS1.
SFVLNLGK 		CPTAC-1440:
LGALS1.
LPDGYEFK
CPTAC-1441:
PDLIM1.
C[+57.0]GTGIVGVFVK 		CPTAC-1442:
PDLIM1.
VAASIGNAQK 		CPTAC-1443:
PDLIM1.
VWSPLVTEEGK
CPTAC-1444:
PDLIM1.
DFEQPLAISR 		CPTAC-1445:
PRDX2.
GLFIIDGK 		CPTAC-1446:
PRDX2.
TDEGIAYR
CPTAC-1447:
PRDX2.
ATAVVDGAFK 		CPTAC-1448:
PRDX4.
DYGVYLEDSGHTLR 		CPTAC-1449:
PRDX4.
LVQAFQYTDK
CPTAC-1450:
PRDX4.
IPLLSDLTHQISK 		CPTAC-1451:
PRDX4.
QITLNDLPVGR 		CPTAC-1452:
S100A1.
ELLQTELSGFLDAQK
CPTAC-1453:
S100A2.
ELPSFVGEK 		CPTAC-1454:
S100B.
AMVALIDVFHQYSGR 		CPTAC-1455:
S100B.
ELINNELSHFLEEIK
CPTAC-1456:
RAD23B.
EQVIAALR 		CPTAC-1457:
RAD23B.
IDIDPEETVK 		CPTAC-1458:
RAD23B.
ILNDDTALK
CPTAC-1459:
SNCG.
EGVVGAVEK 		CPTAC-1460:
SNCG.
ENVVQSVTSVAEK 		CPTAC-1461:
SNCG.
TVEEAENIAVTSGVVR
CPTAC-1462:
TPM1.
LVIIESDLER 		CPTAC-1463:
TPM1.
SIDDLEDELYAQK 		CPTAC-1464:
TPM1.
QLEDELVSLQK
CPTAC-1465:
UBE2C.
YLQETYSK 		CPTAC-1466:
UBE2C.
LSLEFPSGYPYNAPTVK 		CPTAC-1467:
UBE2C.
DPAATSVAAAR
CPTAC-1468:
UBE2C.
GISAFPESDNLFK 		CPTAC-1469:
UBE2I.
DDYPSSPPK 		CPTAC-1470:
UBE2I.
DWRPAITIK
CPTAC-1471:
UBE2I.
DHPFGFVAVPTK

Chromatograms

Data source: Panorama

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view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 4.3 3.6 6.9 11.7 6.1 6.4 12.5 7.1 9.4 15 15 15
y8 (1+) 3.6 3.8 5.5 14.7 5.5 5.8 15.1 6.7 8 15 15 15
y6 (1+) 4 3.9 7.4 13.4 7.2 7.4 14 8.2 10.5 15 15 15
sum 3 3.3 6.2 13 6.1 6.3 13.3 6.9 8.8 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments

Assay Details for CPTAC-1464 Collapse assay details

Data source: Panorama

Official Gene Symbol
TPM1
Peptide Sequence
QLEDELVSLQK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
38
Peptide End
48
CPTAC ID
CPTAC-1464
Peptide Molecular Mass
1,300.6874
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
depleted digested plasma
Submitting Laboratory
Broad Institute
Submitting Lab PI
Steven A. Carr

Publication

View Details (opens in a new window)

Large-Scale Interlaboratory Study to Develop, Analytically Validate and Apply Highly Multiplexed, Quantitative Peptide Assays to Measure Cancer-Relevant Proteins in Plasma. Abbatiello SE, Schilling B, Mani DR, Zimmerman LJ, Hall SC, MacLean B, Albertolle M, Allen S, Burgess M, Cusack MP, Gosh M, Hedrick V, Held JM, Inerowicz HD, Jackson A, Keshishian H, Kinsinger CR, Lyssand J, Makowski L, Mesri M, Rodriguez H, Rudnick P, Sadowski P, Sedransk N, Shaddox K, Skates SJ, Kuhn E, Smith D, Whiteaker JR, Whitwell C, Zhang S, Borchers CH, Fisher SJ, Gibson BW, Liebler DC, MacCoss MJ, Neubert TA, Paulovich AG, Regnier FE, Tempst P, Carr SA. Mol Cell Proteomics. 2015 Sep;14(9):2357-74. doi: 10.1074/mcp.M114.047050. Epub 2015 Feb 18. PMID: 25693799

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Quantiva TSQ
Internal Standard
heavy stable isotope peptide (delta 8-10)
Peptide Standard Purity
>95%
Peptide Standard Label Type
15N
LC
Easy NanoLC1000
Column Packing
Reprosil C18, 1.9um, 200A
Column Dimensions
0.075 x 150 mm
Flow Rate
200 nL/min

Assay Multiplexing Expand assay panel

Broad Institute-Plasma-based Candidate Cancer Biomarkers

CPTAC-1372:
ALDOC.
ALQASALNAWR 		CPTAC-1373:
ALDOC.
AEVNGLAAQGK 		CPTAC-1374:
ALDOC.
ELSDIALR
CPTAC-1375:
ALDOC.
TPSALAILENANVLAR 		CPTAC-1376:
ALDOC.
QVLFSADDR 		CPTAC-1377:
ANXA1.
ALYEAGER
CPTAC-1378:
ANXA1.
GTDVNVFNTILTTR 		CPTAC-1379:
ANXA1.
AAYLQETGKPLDETLK 		CPTAC-1380:
ANXA1.
TPAQFDADELR
CPTAC-1381:
ANXA4.
VLVSLSAGGR 		CPTAC-1382:
ANXA4.
GLGTDEDAIISVLAYR 		CPTAC-1383:
ANXA4.
DEGNYLDDALVR
CPTAC-1384:
ANXA4.
GAGTDEGC[+57.0]LIEILASR 		CPTAC-1385:
ANXA7.
LYQAGEGR 		CPTAC-1386:
ANXA7.
SEIDLVQIK
CPTAC-1387:
ANXA7.
GAGTDDSTLVR 		CPTAC-1388:
ANXA7.
EFSGYVESGLK 		CPTAC-1389:
ANXA7.
GFGTDEQAIVDVVANR
CPTAC-1390:
CALR.
QIDNPDYK 		CPTAC-1391:
CALR.
FYALSASFEPFSNK 		CPTAC-1392:
CALR.
EQFLDGDGWTSR
CPTAC-1393:
CLIC1.
LHIVQVVC[+57.0]K 		CPTAC-1394:
CLIC1.
YLSNAYAR 		CPTAC-1395:
CLIC1.
IEEFLEAVLC[+57.0]PPR
CPTAC-1396:
CLIC1.
GVTFNVTTVDTK 		CPTAC-1397:
CLIC1.
GFTIPEAFR 		CPTAC-1398:
EZR.
IQVWHAEHR
CPTAC-1399:
EZR.
EDEVEEWQHR 		CPTAC-1400:
EZR.
SGYLSSER 		CPTAC-1401:
EZR.
IALLEEAR
CPTAC-1402:
EZR.
SQEQLAAELAEYTAK 		CPTAC-1403:
FSCN1.
FLIVAHDDGR 		CPTAC-1404:
FSCN1.
YLAPSGPSGTLK
CPTAC-1405:
FSCN1.
VTGTLDANR 		CPTAC-1406:
FSCN1.
LSC[+57.0]FAQTVSPAEK 		CPTAC-1407:
FTL.
LGGPEAGLGEYLFER
CPTAC-1408:
FTL.
KPAEDEWGK 		CPTAC-1409:
FEN1.
SIEEIVR 		CPTAC-1410:
FEN1.
QLQQAQAAGAEQEVEK
CPTAC-1411:
FEN1.
LIADVAPSAIR 		CPTAC-1412:
GSTP1.
FQDGDLTLYQSNTILR 		CPTAC-1413:
GSTP1.
EEVVTVETWQEGSLK
CPTAC-1414:
GSTP1.
PPYTVVYFPVR 		CPTAC-1415:
GSTP1.
ASC[+57.0]LYGQLPK 		CPTAC-1416:
GLO1.
FSLYFLAYEDK
CPTAC-1417:
GLO1.
SLDFYTR 		CPTAC-1418:
GLO1.
IAWALSR 		CPTAC-1419:
GLO1.
GFGHIGIAVPDVYSAC[+57.0]K
CPTAC-1420:
GLO1.
FEELGVK 		CPTAC-1421:
GRB2.
FNSLNELVDYHR 		CPTAC-1422:
GRB2.
FGNDVQHFK
CPTAC-1423:
GRB2.
NYVTPVNR 		CPTAC-1424:
GRB2.
ATADDELSFK 		CPTAC-1425:
GRB2.
ESESAPGDFSLSVK
CPTAC-1426:
HSPB1.
LFDQAFGLPR 		CPTAC-1427:
HSPB1.
AQLGGPEAAK 		CPTAC-1428:
HSPB1.
VSLDVNHFAPDELTVK
CPTAC-1429:
HSPB1.
DGVVEITGK 		CPTAC-1430:
HSPB1.
QLSSGVSEIR 		CPTAC-1431:
IL18.
EDELGDR
CPTAC-1432:
IL18.
SDIIFFQR 		CPTAC-1433:
IL18.
TIFIISMYK 		CPTAC-1434:
IL18.
ISTLSC[+57.0]ENK
CPTAC-1435:
IL18.
GMAVTISVK 		CPTAC-1436:
LGALS1.
DGGAWGTEQR 		CPTAC-1437:
LGALS1.
DSNNLC[+57.0]LHFNPR
CPTAC-1438:
LGALS1.
FNAHGDANTIVC[+57.0]NSK 		CPTAC-1439:
LGALS1.
SFVLNLGK 		CPTAC-1440:
LGALS1.
LPDGYEFK
CPTAC-1441:
PDLIM1.
C[+57.0]GTGIVGVFVK 		CPTAC-1442:
PDLIM1.
VAASIGNAQK 		CPTAC-1443:
PDLIM1.
VWSPLVTEEGK
CPTAC-1444:
PDLIM1.
DFEQPLAISR 		CPTAC-1445:
PRDX2.
GLFIIDGK 		CPTAC-1446:
PRDX2.
TDEGIAYR
CPTAC-1447:
PRDX2.
ATAVVDGAFK 		CPTAC-1448:
PRDX4.
DYGVYLEDSGHTLR 		CPTAC-1449:
PRDX4.
LVQAFQYTDK
CPTAC-1450:
PRDX4.
IPLLSDLTHQISK 		CPTAC-1451:
PRDX4.
QITLNDLPVGR 		CPTAC-1452:
S100A1.
ELLQTELSGFLDAQK
CPTAC-1453:
S100A2.
ELPSFVGEK 		CPTAC-1454:
S100B.
AMVALIDVFHQYSGR 		CPTAC-1455:
S100B.
ELINNELSHFLEEIK
CPTAC-1456:
RAD23B.
EQVIAALR 		CPTAC-1457:
RAD23B.
IDIDPEETVK 		CPTAC-1458:
RAD23B.
ILNDDTALK
CPTAC-1459:
SNCG.
EGVVGAVEK 		CPTAC-1460:
SNCG.
ENVVQSVTSVAEK 		CPTAC-1461:
SNCG.
TVEEAENIAVTSGVVR
CPTAC-1462:
TPM1.
LVIIESDLER 		CPTAC-1463:
TPM1.
SIDDLEDELYAQK 		CPTAC-1464:
TPM1.
QLEDELVSLQK
CPTAC-1465:
UBE2C.
YLQETYSK 		CPTAC-1466:
UBE2C.
LSLEFPSGYPYNAPTVK 		CPTAC-1467:
UBE2C.
DPAATSVAAAR
CPTAC-1468:
UBE2C.
GISAFPESDNLFK 		CPTAC-1469:
UBE2I.
DDYPSSPPK 		CPTAC-1470:
UBE2I.
DWRPAITIK
CPTAC-1471:
UBE2I.
DHPFGFVAVPTK

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 6.3 3.3 2.4 17.1 7.6 2.9 18.2 8.3 3.8 15 15 15
y5 (1+) 5 2 1.9 12.7 6.5 4.4 13.6 6.8 4.8 15 15 15
y4 (1+) 3.4 1 1.7 11.8 5.4 2.7 12.3 5.5 3.2 15 15 15
sum 2.1 1.2 1.6 12.3 5.9 2.9 12.5 6 3.3 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments

Assay Details for CPTAC-1463 Collapse assay details

Data source: Panorama

Official Gene Symbol
TPM1
Peptide Sequence
SIDDLEDELYAQK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
252
Peptide End
264
CPTAC ID
CPTAC-1463
Peptide Molecular Mass
1,537.7148
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
depleted digested plasma
Submitting Laboratory
Broad Institute
Submitting Lab PI
Steven A. Carr

Publication

View Details (opens in a new window)

Large-Scale Interlaboratory Study to Develop, Analytically Validate and Apply Highly Multiplexed, Quantitative Peptide Assays to Measure Cancer-Relevant Proteins in Plasma. Abbatiello SE, Schilling B, Mani DR, Zimmerman LJ, Hall SC, MacLean B, Albertolle M, Allen S, Burgess M, Cusack MP, Gosh M, Hedrick V, Held JM, Inerowicz HD, Jackson A, Keshishian H, Kinsinger CR, Lyssand J, Makowski L, Mesri M, Rodriguez H, Rudnick P, Sadowski P, Sedransk N, Shaddox K, Skates SJ, Kuhn E, Smith D, Whiteaker JR, Whitwell C, Zhang S, Borchers CH, Fisher SJ, Gibson BW, Liebler DC, MacCoss MJ, Neubert TA, Paulovich AG, Regnier FE, Tempst P, Carr SA. Mol Cell Proteomics. 2015 Sep;14(9):2357-74. doi: 10.1074/mcp.M114.047050. Epub 2015 Feb 18. PMID: 25693799

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Quantiva TSQ
Internal Standard
heavy stable isotope peptide (delta 8-10)
Peptide Standard Purity
>95%
Peptide Standard Label Type
15N
LC
Easy NanoLC1000
Column Packing
Reprosil C18, 1.9um, 200A
Column Dimensions
0.075 x 150 mm
Flow Rate
200 nL/min

Assay Multiplexing Expand assay panel

Broad Institute-Plasma-based Candidate Cancer Biomarkers

CPTAC-1372:
ALDOC.
ALQASALNAWR 		CPTAC-1373:
ALDOC.
AEVNGLAAQGK 		CPTAC-1374:
ALDOC.
ELSDIALR
CPTAC-1375:
ALDOC.
TPSALAILENANVLAR 		CPTAC-1376:
ALDOC.
QVLFSADDR 		CPTAC-1377:
ANXA1.
ALYEAGER
CPTAC-1378:
ANXA1.
GTDVNVFNTILTTR 		CPTAC-1379:
ANXA1.
AAYLQETGKPLDETLK 		CPTAC-1380:
ANXA1.
TPAQFDADELR
CPTAC-1381:
ANXA4.
VLVSLSAGGR 		CPTAC-1382:
ANXA4.
GLGTDEDAIISVLAYR 		CPTAC-1383:
ANXA4.
DEGNYLDDALVR
CPTAC-1384:
ANXA4.
GAGTDEGC[+57.0]LIEILASR 		CPTAC-1385:
ANXA7.
LYQAGEGR 		CPTAC-1386:
ANXA7.
SEIDLVQIK
CPTAC-1387:
ANXA7.
GAGTDDSTLVR 		CPTAC-1388:
ANXA7.
EFSGYVESGLK 		CPTAC-1389:
ANXA7.
GFGTDEQAIVDVVANR
CPTAC-1390:
CALR.
QIDNPDYK 		CPTAC-1391:
CALR.
FYALSASFEPFSNK 		CPTAC-1392:
CALR.
EQFLDGDGWTSR
CPTAC-1393:
CLIC1.
LHIVQVVC[+57.0]K 		CPTAC-1394:
CLIC1.
YLSNAYAR 		CPTAC-1395:
CLIC1.
IEEFLEAVLC[+57.0]PPR
CPTAC-1396:
CLIC1.
GVTFNVTTVDTK 		CPTAC-1397:
CLIC1.
GFTIPEAFR 		CPTAC-1398:
EZR.
IQVWHAEHR
CPTAC-1399:
EZR.
EDEVEEWQHR 		CPTAC-1400:
EZR.
SGYLSSER 		CPTAC-1401:
EZR.
IALLEEAR
CPTAC-1402:
EZR.
SQEQLAAELAEYTAK 		CPTAC-1403:
FSCN1.
FLIVAHDDGR 		CPTAC-1404:
FSCN1.
YLAPSGPSGTLK
CPTAC-1405:
FSCN1.
VTGTLDANR 		CPTAC-1406:
FSCN1.
LSC[+57.0]FAQTVSPAEK 		CPTAC-1407:
FTL.
LGGPEAGLGEYLFER
CPTAC-1408:
FTL.
KPAEDEWGK 		CPTAC-1409:
FEN1.
SIEEIVR 		CPTAC-1410:
FEN1.
QLQQAQAAGAEQEVEK
CPTAC-1411:
FEN1.
LIADVAPSAIR 		CPTAC-1412:
GSTP1.
FQDGDLTLYQSNTILR 		CPTAC-1413:
GSTP1.
EEVVTVETWQEGSLK
CPTAC-1414:
GSTP1.
PPYTVVYFPVR 		CPTAC-1415:
GSTP1.
ASC[+57.0]LYGQLPK 		CPTAC-1416:
GLO1.
FSLYFLAYEDK
CPTAC-1417:
GLO1.
SLDFYTR 		CPTAC-1418:
GLO1.
IAWALSR 		CPTAC-1419:
GLO1.
GFGHIGIAVPDVYSAC[+57.0]K
CPTAC-1420:
GLO1.
FEELGVK 		CPTAC-1421:
GRB2.
FNSLNELVDYHR 		CPTAC-1422:
GRB2.
FGNDVQHFK
CPTAC-1423:
GRB2.
NYVTPVNR 		CPTAC-1424:
GRB2.
ATADDELSFK 		CPTAC-1425:
GRB2.
ESESAPGDFSLSVK
CPTAC-1426:
HSPB1.
LFDQAFGLPR 		CPTAC-1427:
HSPB1.
AQLGGPEAAK 		CPTAC-1428:
HSPB1.
VSLDVNHFAPDELTVK
CPTAC-1429:
HSPB1.
DGVVEITGK 		CPTAC-1430:
HSPB1.
QLSSGVSEIR 		CPTAC-1431:
IL18.
EDELGDR
CPTAC-1432:
IL18.
SDIIFFQR 		CPTAC-1433:
IL18.
TIFIISMYK 		CPTAC-1434:
IL18.
ISTLSC[+57.0]ENK
CPTAC-1435:
IL18.
GMAVTISVK 		CPTAC-1436:
LGALS1.
DGGAWGTEQR 		CPTAC-1437:
LGALS1.
DSNNLC[+57.0]LHFNPR
CPTAC-1438:
LGALS1.
FNAHGDANTIVC[+57.0]NSK 		CPTAC-1439:
LGALS1.
SFVLNLGK 		CPTAC-1440:
LGALS1.
LPDGYEFK
CPTAC-1441:
PDLIM1.
C[+57.0]GTGIVGVFVK 		CPTAC-1442:
PDLIM1.
VAASIGNAQK 		CPTAC-1443:
PDLIM1.
VWSPLVTEEGK
CPTAC-1444:
PDLIM1.
DFEQPLAISR 		CPTAC-1445:
PRDX2.
GLFIIDGK 		CPTAC-1446:
PRDX2.
TDEGIAYR
CPTAC-1447:
PRDX2.
ATAVVDGAFK 		CPTAC-1448:
PRDX4.
DYGVYLEDSGHTLR 		CPTAC-1449:
PRDX4.
LVQAFQYTDK
CPTAC-1450:
PRDX4.
IPLLSDLTHQISK 		CPTAC-1451:
PRDX4.
QITLNDLPVGR 		CPTAC-1452:
S100A1.
ELLQTELSGFLDAQK
CPTAC-1453:
S100A2.
ELPSFVGEK 		CPTAC-1454:
S100B.
AMVALIDVFHQYSGR 		CPTAC-1455:
S100B.
ELINNELSHFLEEIK
CPTAC-1456:
RAD23B.
EQVIAALR 		CPTAC-1457:
RAD23B.
IDIDPEETVK 		CPTAC-1458:
RAD23B.
ILNDDTALK
CPTAC-1459:
SNCG.
EGVVGAVEK 		CPTAC-1460:
SNCG.
ENVVQSVTSVAEK 		CPTAC-1461:
SNCG.
TVEEAENIAVTSGVVR
CPTAC-1462:
TPM1.
LVIIESDLER 		CPTAC-1463:
TPM1.
SIDDLEDELYAQK 		CPTAC-1464:
TPM1.
QLEDELVSLQK
CPTAC-1465:
UBE2C.
YLQETYSK 		CPTAC-1466:
UBE2C.
LSLEFPSGYPYNAPTVK 		CPTAC-1467:
UBE2C.
DPAATSVAAAR
CPTAC-1468:
UBE2C.
GISAFPESDNLFK 		CPTAC-1469:
UBE2I.
DDYPSSPPK 		CPTAC-1470:
UBE2I.
DWRPAITIK
CPTAC-1471:
UBE2I.
DHPFGFVAVPTK

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y11 (1+) 8.4 3.5 1.7 18.9 7.4 3 20.7 8.2 3.4 15 15 15
y7 (1+) 7.6 1.6 2.4 19.7 6.9 3.3 21.1 7.1 4.1 15 15 15
y8 (1+) 6.6 1.8 1.9 18.9 7.8 3.4 20 8 3.9 15 15 15
sum 5.2 1.4 1.5 18.6 7.2 2.8 19.3 7.3 3.2 15 15 15

Additional Resources and Comments

Resources
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Comments

Assay Details for non-CPTAC-3624 Collapse assay details

Data source: Panorama

Official Gene Symbol
Tpm1
Peptide Sequence
LVIIESDLER
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
169
Peptide End
178
CPTAC ID
non-CPTAC-3624
Peptide Molecular Mass
1,185.6605
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Assay Multiplexing Expand assay panel

Broad Institute-Plasma-based Candidate Cancer Biomarkers

CPTAC-1372:
ALDOC.
ALQASALNAWR 		CPTAC-1373:
ALDOC.
AEVNGLAAQGK 		CPTAC-1374:
ALDOC.
ELSDIALR
CPTAC-1375:
ALDOC.
TPSALAILENANVLAR 		CPTAC-1376:
ALDOC.
QVLFSADDR 		CPTAC-1377:
ANXA1.
ALYEAGER
CPTAC-1378:
ANXA1.
GTDVNVFNTILTTR 		CPTAC-1379:
ANXA1.
AAYLQETGKPLDETLK 		CPTAC-1380:
ANXA1.
TPAQFDADELR
CPTAC-1381:
ANXA4.
VLVSLSAGGR 		CPTAC-1382:
ANXA4.
GLGTDEDAIISVLAYR 		CPTAC-1383:
ANXA4.
DEGNYLDDALVR
CPTAC-1384:
ANXA4.
GAGTDEGC[+57.0]LIEILASR 		CPTAC-1385:
ANXA7.
LYQAGEGR 		CPTAC-1386:
ANXA7.
SEIDLVQIK
CPTAC-1387:
ANXA7.
GAGTDDSTLVR 		CPTAC-1388:
ANXA7.
EFSGYVESGLK 		CPTAC-1389:
ANXA7.
GFGTDEQAIVDVVANR
CPTAC-1390:
CALR.
QIDNPDYK 		CPTAC-1391:
CALR.
FYALSASFEPFSNK 		CPTAC-1392:
CALR.
EQFLDGDGWTSR
CPTAC-1393:
CLIC1.
LHIVQVVC[+57.0]K 		CPTAC-1394:
CLIC1.
YLSNAYAR 		CPTAC-1395:
CLIC1.
IEEFLEAVLC[+57.0]PPR
CPTAC-1396:
CLIC1.
GVTFNVTTVDTK 		CPTAC-1397:
CLIC1.
GFTIPEAFR 		CPTAC-1398:
EZR.
IQVWHAEHR
CPTAC-1399:
EZR.
EDEVEEWQHR 		CPTAC-1400:
EZR.
SGYLSSER 		CPTAC-1401:
EZR.
IALLEEAR
CPTAC-1402:
EZR.
SQEQLAAELAEYTAK 		CPTAC-1403:
FSCN1.
FLIVAHDDGR 		CPTAC-1404:
FSCN1.
YLAPSGPSGTLK
CPTAC-1405:
FSCN1.
VTGTLDANR 		CPTAC-1406:
FSCN1.
LSC[+57.0]FAQTVSPAEK 		CPTAC-1407:
FTL.
LGGPEAGLGEYLFER
CPTAC-1408:
FTL.
KPAEDEWGK 		CPTAC-1409:
FEN1.
SIEEIVR 		CPTAC-1410:
FEN1.
QLQQAQAAGAEQEVEK
CPTAC-1411:
FEN1.
LIADVAPSAIR 		CPTAC-1412:
GSTP1.
FQDGDLTLYQSNTILR 		CPTAC-1413:
GSTP1.
EEVVTVETWQEGSLK
CPTAC-1414:
GSTP1.
PPYTVVYFPVR 		CPTAC-1415:
GSTP1.
ASC[+57.0]LYGQLPK 		CPTAC-1416:
GLO1.
FSLYFLAYEDK
CPTAC-1417:
GLO1.
SLDFYTR 		CPTAC-1418:
GLO1.
IAWALSR 		CPTAC-1419:
GLO1.
GFGHIGIAVPDVYSAC[+57.0]K
CPTAC-1420:
GLO1.
FEELGVK 		CPTAC-1421:
GRB2.
FNSLNELVDYHR 		CPTAC-1422:
GRB2.
FGNDVQHFK
CPTAC-1423:
GRB2.
NYVTPVNR 		CPTAC-1424:
GRB2.
ATADDELSFK 		CPTAC-1425:
GRB2.
ESESAPGDFSLSVK
CPTAC-1426:
HSPB1.
LFDQAFGLPR 		CPTAC-1427:
HSPB1.
AQLGGPEAAK 		CPTAC-1428:
HSPB1.
VSLDVNHFAPDELTVK
CPTAC-1429:
HSPB1.
DGVVEITGK 		CPTAC-1430:
HSPB1.
QLSSGVSEIR 		CPTAC-1431:
IL18.
EDELGDR
CPTAC-1432:
IL18.
SDIIFFQR 		CPTAC-1433:
IL18.
TIFIISMYK 		CPTAC-1434:
IL18.
ISTLSC[+57.0]ENK
CPTAC-1435:
IL18.
GMAVTISVK 		CPTAC-1436:
LGALS1.
DGGAWGTEQR 		CPTAC-1437:
LGALS1.
DSNNLC[+57.0]LHFNPR
CPTAC-1438:
LGALS1.
FNAHGDANTIVC[+57.0]NSK 		CPTAC-1439:
LGALS1.
SFVLNLGK 		CPTAC-1440:
LGALS1.
LPDGYEFK
CPTAC-1441:
PDLIM1.
C[+57.0]GTGIVGVFVK 		CPTAC-1442:
PDLIM1.
VAASIGNAQK 		CPTAC-1443:
PDLIM1.
VWSPLVTEEGK
CPTAC-1444:
PDLIM1.
DFEQPLAISR 		CPTAC-1445:
PRDX2.
GLFIIDGK 		CPTAC-1446:
PRDX2.
TDEGIAYR
CPTAC-1447:
PRDX2.
ATAVVDGAFK 		CPTAC-1448:
PRDX4.
DYGVYLEDSGHTLR 		CPTAC-1449:
PRDX4.
LVQAFQYTDK
CPTAC-1450:
PRDX4.
IPLLSDLTHQISK 		CPTAC-1451:
PRDX4.
QITLNDLPVGR 		CPTAC-1452:
S100A1.
ELLQTELSGFLDAQK
CPTAC-1453:
S100A2.
ELPSFVGEK 		CPTAC-1454:
S100B.
AMVALIDVFHQYSGR 		CPTAC-1455:
S100B.
ELINNELSHFLEEIK
CPTAC-1456:
RAD23B.
EQVIAALR 		CPTAC-1457:
RAD23B.
IDIDPEETVK 		CPTAC-1458:
RAD23B.
ILNDDTALK
CPTAC-1459:
SNCG.
EGVVGAVEK 		CPTAC-1460:
SNCG.
ENVVQSVTSVAEK 		CPTAC-1461:
SNCG.
TVEEAENIAVTSGVVR
CPTAC-1462:
TPM1.
LVIIESDLER 		CPTAC-1463:
TPM1.
SIDDLEDELYAQK 		CPTAC-1464:
TPM1.
QLEDELVSLQK
CPTAC-1465:
UBE2C.
YLQETYSK 		CPTAC-1466:
UBE2C.
LSLEFPSGYPYNAPTVK 		CPTAC-1467:
UBE2C.
DPAATSVAAAR
CPTAC-1468:
UBE2C.
GISAFPESDNLFK 		CPTAC-1469:
UBE2I.
DDYPSSPPK 		CPTAC-1470:
UBE2I.
DWRPAITIK
CPTAC-1471:
UBE2I.
DHPFGFVAVPTK

Chromatograms

Data source: Panorama

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view larger image

Response Curves

Data source: Panorama

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Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b2 (1+) 18.5 3.2 3 24.1 10.8 7.1 30.4 11.3 7.7 15 15 15
y8 (2+) 18.2 5.7 3.9 25.6 9.9 10 31.4 11.4 10.7 15 15 15
y6 (1+) 12.6 4.2 2.5 17 9 8.9 21.2 9.9 9.2 15 15 15
y7 (1+) 12.4 2.7 2.4 15.2 8.6 10.3 19.6 9 10.6 15 15 15
y8 (1+) 14.3 5.4 3.5 12.8 9.1 8.5 19.2 10.6 9.2 15 15 15
sum 9.8 1.7 1.8 16.3 9 8.3 19 9.2 8.5 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments