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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

S100A1

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
S100A1 S100A
Sequence Length (AA) Molecular Weight (Da)
94 10546
Protein Name
Protein S100-A1
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MGSELETAME TLINVFHAHS GKEGDKYKLS KKELKELLQT ELSGFLDAQK
60 70 80 90 94
DVDAVDKVMK ELDENGDGEV DFQEYVVLVA ALTVACNNFF WENS

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt


Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1452 Collapse assay details

Data source: Panorama

Official Gene Symbol
S100A1
Peptide Sequence
ELLQTELSGFLDAQK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
36
Peptide End
50
CPTAC ID
CPTAC-1452
Peptide Molecular Mass
1,690.8778
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
depleted digested plasma
Submitting Laboratory
Broad Institute
Submitting Lab PI
Steven A. Carr

Publication

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Large-Scale Interlaboratory Study to Develop, Analytically Validate and Apply Highly Multiplexed, Quantitative Peptide Assays to Measure Cancer-Relevant Proteins in Plasma. Abbatiello SE, Schilling B, Mani DR, Zimmerman LJ, Hall SC, MacLean B, Albertolle M, Allen S, Burgess M, Cusack MP, Gosh M, Hedrick V, Held JM, Inerowicz HD, Jackson A, Keshishian H, Kinsinger CR, Lyssand J, Makowski L, Mesri M, Rodriguez H, Rudnick P, Sadowski P, Sedransk N, Shaddox K, Skates SJ, Kuhn E, Smith D, Whiteaker JR, Whitwell C, Zhang S, Borchers CH, Fisher SJ, Gibson BW, Liebler DC, MacCoss MJ, Neubert TA, Paulovich AG, Regnier FE, Tempst P, Carr SA. Mol Cell Proteomics. 2015 Sep;14(9):2357-74. doi: 10.1074/mcp.M114.047050. Epub 2015 Feb 18. PMID: 25693799

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Quantiva TSQ
Internal Standard
heavy stable isotope peptide (delta 8-10)
Peptide Standard Purity
>95%
Peptide Standard Label Type
15N
LC
Easy NanoLC1000
Column Packing
Reprosil C18, 1.9um, 200A
Column Dimensions
0.075 x 150 mm
Flow Rate
200 nL/min

Assay Multiplexing Expand assay panel

Broad Institute-Plasma-based Candidate Cancer Biomarkers

CPTAC-1372:
ALDOC.
ALQASALNAWR 		CPTAC-1373:
ALDOC.
AEVNGLAAQGK 		CPTAC-1374:
ALDOC.
ELSDIALR
CPTAC-1375:
ALDOC.
TPSALAILENANVLAR 		CPTAC-1376:
ALDOC.
QVLFSADDR 		CPTAC-1377:
ANXA1.
ALYEAGER
CPTAC-1378:
ANXA1.
GTDVNVFNTILTTR 		CPTAC-1379:
ANXA1.
AAYLQETGKPLDETLK 		CPTAC-1380:
ANXA1.
TPAQFDADELR
CPTAC-1381:
ANXA4.
VLVSLSAGGR 		CPTAC-1382:
ANXA4.
GLGTDEDAIISVLAYR 		CPTAC-1383:
ANXA4.
DEGNYLDDALVR
CPTAC-1384:
ANXA4.
GAGTDEGC[+57.0]LIEILASR 		CPTAC-1385:
ANXA7.
LYQAGEGR 		CPTAC-1386:
ANXA7.
SEIDLVQIK
CPTAC-1387:
ANXA7.
GAGTDDSTLVR 		CPTAC-1388:
ANXA7.
EFSGYVESGLK 		CPTAC-1389:
ANXA7.
GFGTDEQAIVDVVANR
CPTAC-1390:
CALR.
QIDNPDYK 		CPTAC-1391:
CALR.
FYALSASFEPFSNK 		CPTAC-1392:
CALR.
EQFLDGDGWTSR
CPTAC-1393:
CLIC1.
LHIVQVVC[+57.0]K 		CPTAC-1394:
CLIC1.
YLSNAYAR 		CPTAC-1395:
CLIC1.
IEEFLEAVLC[+57.0]PPR
CPTAC-1396:
CLIC1.
GVTFNVTTVDTK 		CPTAC-1397:
CLIC1.
GFTIPEAFR 		CPTAC-1398:
EZR.
IQVWHAEHR
CPTAC-1399:
EZR.
EDEVEEWQHR 		CPTAC-1400:
EZR.
SGYLSSER 		CPTAC-1401:
EZR.
IALLEEAR
CPTAC-1402:
EZR.
SQEQLAAELAEYTAK 		CPTAC-1403:
FSCN1.
FLIVAHDDGR 		CPTAC-1404:
FSCN1.
YLAPSGPSGTLK
CPTAC-1405:
FSCN1.
VTGTLDANR 		CPTAC-1406:
FSCN1.
LSC[+57.0]FAQTVSPAEK 		CPTAC-1407:
FTL.
LGGPEAGLGEYLFER
CPTAC-1408:
FTL.
KPAEDEWGK 		CPTAC-1409:
FEN1.
SIEEIVR 		CPTAC-1410:
FEN1.
QLQQAQAAGAEQEVEK
CPTAC-1411:
FEN1.
LIADVAPSAIR 		CPTAC-1412:
GSTP1.
FQDGDLTLYQSNTILR 		CPTAC-1413:
GSTP1.
EEVVTVETWQEGSLK
CPTAC-1414:
GSTP1.
PPYTVVYFPVR 		CPTAC-1415:
GSTP1.
ASC[+57.0]LYGQLPK 		CPTAC-1416:
GLO1.
FSLYFLAYEDK
CPTAC-1417:
GLO1.
SLDFYTR 		CPTAC-1418:
GLO1.
IAWALSR 		CPTAC-1419:
GLO1.
GFGHIGIAVPDVYSAC[+57.0]K
CPTAC-1420:
GLO1.
FEELGVK 		CPTAC-1421:
GRB2.
FNSLNELVDYHR 		CPTAC-1422:
GRB2.
FGNDVQHFK
CPTAC-1423:
GRB2.
NYVTPVNR 		CPTAC-1424:
GRB2.
ATADDELSFK 		CPTAC-1425:
GRB2.
ESESAPGDFSLSVK
CPTAC-1426:
HSPB1.
LFDQAFGLPR 		CPTAC-1427:
HSPB1.
AQLGGPEAAK 		CPTAC-1428:
HSPB1.
VSLDVNHFAPDELTVK
CPTAC-1429:
HSPB1.
DGVVEITGK 		CPTAC-1430:
HSPB1.
QLSSGVSEIR 		CPTAC-1431:
IL18.
EDELGDR
CPTAC-1432:
IL18.
SDIIFFQR 		CPTAC-1433:
IL18.
TIFIISMYK 		CPTAC-1434:
IL18.
ISTLSC[+57.0]ENK
CPTAC-1435:
IL18.
GMAVTISVK 		CPTAC-1436:
LGALS1.
DGGAWGTEQR 		CPTAC-1437:
LGALS1.
DSNNLC[+57.0]LHFNPR
CPTAC-1438:
LGALS1.
FNAHGDANTIVC[+57.0]NSK 		CPTAC-1439:
LGALS1.
SFVLNLGK 		CPTAC-1440:
LGALS1.
LPDGYEFK
CPTAC-1441:
PDLIM1.
C[+57.0]GTGIVGVFVK 		CPTAC-1442:
PDLIM1.
VAASIGNAQK 		CPTAC-1443:
PDLIM1.
VWSPLVTEEGK
CPTAC-1444:
PDLIM1.
DFEQPLAISR 		CPTAC-1445:
PRDX2.
GLFIIDGK 		CPTAC-1446:
PRDX2.
TDEGIAYR
CPTAC-1447:
PRDX2.
ATAVVDGAFK 		CPTAC-1448:
PRDX4.
DYGVYLEDSGHTLR 		CPTAC-1449:
PRDX4.
LVQAFQYTDK
CPTAC-1450:
PRDX4.
IPLLSDLTHQISK 		CPTAC-1451:
PRDX4.
QITLNDLPVGR 		CPTAC-1452:
S100A1.
ELLQTELSGFLDAQK
CPTAC-1453:
S100A2.
ELPSFVGEK 		CPTAC-1454:
S100B.
AMVALIDVFHQYSGR 		CPTAC-1455:
S100B.
ELINNELSHFLEEIK
CPTAC-1456:
RAD23B.
EQVIAALR 		CPTAC-1457:
RAD23B.
IDIDPEETVK 		CPTAC-1458:
RAD23B.
ILNDDTALK
CPTAC-1459:
SNCG.
EGVVGAVEK 		CPTAC-1460:
SNCG.
ENVVQSVTSVAEK 		CPTAC-1461:
SNCG.
TVEEAENIAVTSGVVR
CPTAC-1462:
TPM1.
LVIIESDLER 		CPTAC-1463:
TPM1.
SIDDLEDELYAQK 		CPTAC-1464:
TPM1.
QLEDELVSLQK
CPTAC-1465:
UBE2C.
YLQETYSK 		CPTAC-1466:
UBE2C.
LSLEFPSGYPYNAPTVK 		CPTAC-1467:
UBE2C.
DPAATSVAAAR
CPTAC-1468:
UBE2C.
GISAFPESDNLFK 		CPTAC-1469:
UBE2I.
DDYPSSPPK 		CPTAC-1470:
UBE2I.
DWRPAITIK
CPTAC-1471:
UBE2I.
DHPFGFVAVPTK

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y9 (1+) 11.4 7.2 5.5 21 11 8.2 23.9 13.1 9.9 15 15 15
y8 (1+) 7.1 7.7 5.2 18 9.2 5.5 19.3 12 7.6 15 15 15
y11 (1+) 5.8 6.2 6.7 16.7 9.9 8.3 17.7 11.7 10.7 15 15 15
sum 5.3 6.1 3.8 18 9.3 5.2 18.8 11.1 6.4 15 15 15

Additional Resources and Comments

Resources
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