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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

DOCK7

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
DOCK7 KIAA1771
Sequence Length (AA) Molecular Weight (Da)
2140 242561
Protein Name
Dedicator of cytokinesis protein 7
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MAERRAFAQK ISRTVAAEVR KQISGQYSGS PQLLKNLNIV GNISHHTTVP
60 70 80 90 100
LTEAVDPVDL EDYLITHPLA VDSGPLRDLI EFPPDDIEVV YSPRDCRTLV
110 120 130 140 150
SAVPEESEMD PHVRDCIRSY TEDWAIVIRK YHKLGTGFNP NTLDKQKERQ
160 170 180 190 200
KGLPKQVFES DEAPDGNSYQ DDQDDLKRRS MSIDDTPRGS WACSIFDLKN
210 220 230 240 250
SLPDALLPNL LDRTPNEEID RQNDDQRKSN RHKELFALHP SPDEEEPIER
260 270 280 290 300
LSVPDIPKEH FGQRLLVKCL SLKFEIEIEP IFASLALYDV KEKKKISENF
310 320 330 340 350
YFDLNSEQMK GLLRPHVPPA AITTLARSAI FSITYPSQDV FLVIKLEKVL
360 370 380 390 400
QQGDIGECAE PYMIFKEADA TKNKEKLEKL KSQADQFCQR LGKYRMPFAW
410 420 430 440 450
TAIHLMNIVS SAGSLERDST EVEISTGERK GSWSERRNSS IVGRRSLERT
460 470 480 490 500
TSGDDACNLT SFRPATLTVT NFFKQEGDRL SDEDLYKFLA DMRRPSSVLR
510 520 530 540 550
RLRPITAQLK IDISPAPENP HYCLTPELLQ VKLYPDSRVR PTREILEFPA
560 570 580 590 600
RDVYVPNTTY RNLLYIYPQS LNFANRQGSA RNITVKVQFM YGEDPSNAMP
610 620 630 640 650
VIFGKSSCSE FSKEAYTAVV YHNRSPDFHE EIKVKLPATL TDHHHLLFTF
660 670 680 690 700
YHVSCQQKQN TPLETPVGYT WIPMLQNGRL KTGQFCLPVS LEKPPQAYSV
710 720 730 740 750
LSPEVPLPGM KWVDNHKGVF NVEVVAVSSI HTQDPYLDKF FALVNALDEH
760 770 780 790 800
LFPVRIGDMR IMENNLENEL KSSISALNSS QLEPVVRFLH LLLDKLILLV
810 820 830 840 850
IRPPVIAGQI VNLGQASFEA MASIINRLHK NLEGNHDQHG RNSLLASYIH
860 870 880 890 900
YVFRLPNTYP NSSSPGPGGL GGSVHYATMA RSAVRPASLN LNRSRSLSNS
910 920 930 940 950
NPDISGTPTS PDDEVRSIIG SKGLDRSNSW VNTGGPKAAP WGSNPSPSAE
960 970 980 990 1000
STQAMDRSCN RMSSHTETSS FLQTLTGRLP TKKLFHEELA LQWVVCSGSV
1010 1020 1030 1040 1050
RESALQQAWF FFELMVKSMV HHLYFNDKLE APRKSRFPER FMDDIAALVS
1060 1070 1080 1090 1100
TIASDIVSRF QKDTEMVERL NTSLAFFLND LLSVMDRGFV FSLIKSCYKQ
1110 1120 1130 1140 1150
VSSKLYSLPN PSVLVSLRLD FLRIICSHEH YVTLNLPCSL LTPPASPSPS
1160 1170 1180 1190 1200
VSSATSQSSG FSTNVQDQKI ANMFELSVPF RQQHYLAGLV LTELAVILDP
1210 1220 1230 1240 1250
DAEGLFGLHK KVINMVHNLL SSHDSDPRYS DPQIKARVAM LYLPLIGIIM
1260 1270 1280 1290 1300
ETVPQLYDFT ETHNQRGRPI CIATDDYESE SGSMISQTVA MAIAGTSVPQ
1310 1320 1330 1340 1350
LTRPGSFLLT STSGRQHTTF SAESSRSLLI CLLWVLKNAD ETVLQKWFTD
1360 1370 1380 1390 1400
LSVLQLNRLL DLLYLCVSCF EYKGKKVFER MNSLTFKKSK DMRAKLEEAI
1410 1420 1430 1440 1450
LGSIGARQEM VRRSRGQLGT YTIASPPERS PSGSAFGSQE NLRWRKDMTH
1460 1470 1480 1490 1500
WRQNTEKLDK SRAEIEHEAL IDGNLATEAN LIILDTLEIV VQTVSVTESK
1510 1520 1530 1540 1550
ESILGGVLKV LLHSMACNQS AVYLQHCFAT QRALVSKFPE LLFEEETEQC
1560 1570 1580 1590 1600
ADLCLRLLRH CSSSIGTIRS HASASLYLLM RQNFEIGNNF ARVKMQVTMS
1610 1620 1630 1640 1650
LSSLVGTSQN FNEEFLRRSL KTILTYAEED LELRETTFPD QVQDLVFNLH
1660 1670 1680 1690 1700
MILSDTVKMK EHQEDPEMLI DLMYRIAKGY QTSPDLRLTW LQNMAGKHSE
1710 1720 1730 1740 1750
RSNHAEAAQC LVHSAALVAE YLSMLEDRKY LPVGCVTFQN ISSNVLEESA
1760 1770 1780 1790 1800
VSDDVVSPDE EGICSGKYFT ESGLVGLLEQ AAASFSMAGM YEAVNEVYKV
1810 1820 1830 1840 1850
LIPIHEANRD AKKLSTIHGK LQEAFSKIVH QSTGWERMFG TYFRVGFYGT
1860 1870 1880 1890 1900
KFGDLDEQEF VYKEPAITKL AEISHRLEGF YGERFGEDVV EVIKDSNPVD
1910 1920 1930 1940 1950
KCKLDPNKAY IQITYVEPYF DTYEMKDRIT YFDKNYNLRR FMYCTPFTLD
1960 1970 1980 1990 2000
GRAHGELHEQ FKRKTILTTS HAFPYIKTRV NVTHKEEIIL TPIEVAIEDM
2010 2020 2030 2040 2050
QKKTQELAFA THQDPADPKM LQMVLQGSVG TTVNQGPLEV AQVFLSEIPS
2060 2070 2080 2090 2100
DPKLFRHHNK LRLCFKDFTK RCEDALRKNK SLIGPDQKEY QRELERNYHR
2110 2120 2130 2140
LKEALQPLIN RKIPQLYKAV LPVTCHRDSF SRMSLRKMDL

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt


Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1732 Collapse assay details

Data source: Panorama

Official Gene Symbol
DOCK7
Peptide Modified Sequence
AVLPVT[+80.0]C[+57.0]HR
Modification Type
Phospho (ST), Carbamidomethyl (C)
Protein - Site of Modification
2124, 2125
Peptide - Site of Modification
6, 7
Peptide Start
2119
Peptide End
2127
CPTAC ID
CPTAC-1732
Peptide Molecular Mass
1,131.5260
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM or SRM
Enrichment Method
Titanium Dioxide
Matrix
Ovarian cancer cell lysate
Submitting Laboratory
Pacific Northwest National Laboratory
Submitting Lab PI
Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Waters nanoACQUITY (Part Number 176016000)
Column Packing
Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions
100 um x 100 mm
Flow Rate
0.5 uL/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-TiO2SRM

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (2+) 7.7 5.8 4.4 12.3 4.9 6 14.5 7.6 7.4 15 15 15
y7 (2+) 19.6 5.8 6.6 22.3 5.9 7.8 29.7 8.3 10.2 15 15 15
y6 (1+) 6.9 4.6 5.1 10.4 4.2 7.3 12.5 6.2 8.9 15 15 15
sum 7.7 3.3 3.2 11.8 3.4 4.5 14.1 4.7 5.5 15 15 15

Additional Resources and Comments

Resources
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Assay Details for CPTAC-1729 Collapse assay details

Data source: Panorama

Official Gene Symbol
DOCK7
Peptide Modified Sequence
LGTGFNPNT[+80.0]LDK
Modification Type
Phospho (ST)
Protein - Site of Modification
142
Peptide - Site of Modification
9
Peptide Start
134
Peptide End
145
CPTAC ID
CPTAC-1729
Peptide Molecular Mass
1,355.6122
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM or SRM
Enrichment Method
Titanium Dioxide
Matrix
Ovarian cancer cell lysate
Submitting Laboratory
Pacific Northwest National Laboratory
Submitting Lab PI
Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Waters nanoACQUITY (Part Number 176016000)
Column Packing
Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions
100 um x 100 mm
Flow Rate
0.5 uL/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-TiO2SRM

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 9.3 5.1 4.6 11.8 5.6 5.6 15 7.6 7.2 15 15 15
y7 (1+) 24.3 7.4 5.4 23 7.1 5.3 33.5 10.3 7.6 15 15 15
y8 (1+) 22.2 10.3 10.2 27.7 9.7 11.1 35.5 14.1 15.1 15 15 15
sum 7.7 4.1 3.6 9.8 4.3 4.6 12.5 5.9 5.8 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments

Assay Details for CPTAC-1730 Collapse assay details

Data source: Panorama

Official Gene Symbol
DOCK7
Peptide Modified Sequence
MNS[+80.0]LTFK
Modification Type
Phospho (ST)
Protein - Site of Modification
1383
Peptide - Site of Modification
3
Peptide Start
1381
Peptide End
1387
CPTAC ID
CPTAC-1730
Peptide Molecular Mass
919.3875
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM or SRM
Enrichment Method
Titanium Dioxide
Matrix
Ovarian cancer cell lysate
Submitting Laboratory
Pacific Northwest National Laboratory
Submitting Lab PI
Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Waters nanoACQUITY (Part Number 176016000)
Column Packing
Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions
100 um x 100 mm
Flow Rate
0.5 uL/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-TiO2SRM

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y2 (1+) 34 18.9 12.3 29.7 22.7 11.5 45.1 29.5 16.8 15 15 15
y5 (1+) 32.9 26.8 16.1 32.6 27.8 17.7 46.3 38.6 23.9 15 15 15
y6 (1+) 22.8 17.2 11.4 24.7 17.1 13.3 33.6 24.3 17.5 15 15 15
sum 15 12.2 8.3 15.8 13 8.7 21.8 17.8 12 15 15 15

Additional Resources and Comments

Resources
Download SOPs
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Comments

Assay Details for CPTAC-1731 Collapse assay details

Data source: Panorama

Official Gene Symbol
DOCK7
Peptide Modified Sequence
SPS[+80.0]GSAFGSQENLR
Modification Type
Phospho (ST)
Protein - Site of Modification
1432
Peptide - Site of Modification
3
Peptide Start
1430
Peptide End
1443
CPTAC ID
CPTAC-1731
Peptide Molecular Mass
1,515.6355
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM or SRM
Enrichment Method
Titanium Dioxide
Matrix
Ovarian cancer cell lysate
Submitting Laboratory
Pacific Northwest National Laboratory
Submitting Lab PI
Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Waters nanoACQUITY (Part Number 176016000)
Column Packing
Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions
100 um x 100 mm
Flow Rate
0.5 uL/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-TiO2SRM

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 13 4.6 4.5 15.2 5 6 20 6.8 7.5 15 15 15
y8 (1+) 15.3 3.8 3.3 12.7 4.3 7.2 19.9 5.7 7.9 15 15 15
y9 (1+) 8.2 5.1 7.4 9.7 8.4 6.2 12.7 9.8 9.7 15 15 15
sum 8.6 3.5 2.1 9.4 4.5 5.2 12.7 5.7 5.6 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments