NFATC4, CPTAC-1749 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
NFATC4	NFAT3

Sequence Length (AA)	Molecular Weight (Da)
902	95449

Protein Name
Nuclear factor of activated T-cells, cytoplasmic 4

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MGAASCEDEE	LEFKLVFGEE	KEAPPLGAGG	LGEELDSEDA	PPCCRLALGE
60	70	80	90	100
PPPYGAAPIG	IPRPPPPRPG	MHSPPPRPAP	SPGTWESQPA	RSVRLGGPGG
110	120	130	140	150
GAGGAGGGRV	LECPSIRITS	ISPTPEPPAA	LEDNPDAWGD	GSPRDYPPPE
160	170	180	190	200
GFGGYREAGG	QGGGAFFSPS	PGSSSLSSWS	FFSDASDEAA	LYAACDEVES
210	220	230	240	250
ELNEAASRFG	LGSPLPSPRA	SPRPWTPEDP	WSLYGPSPGG	RGPEDSWLLL
260	270	280	290	300
SAPGPTPASP	RPASPCGKRR	YSSSGTPSSA	SPALSRRGSL	GEEGSEPPPP
310	320	330	340	350
PPLPLARDPG	SPGPFDYVGA	PPAESIPQKT	RRTSSEQAVA	LPRSEEPASC
360	370	380	390	400
NGKLPLGAEE	SVAPPGGSRK	EVAGMDYLAV	PSPLAWSKAR	IGGHSPIFRT
410	420	430	440	450
SALPPLDWPL	PSQYEQLELR	IEVQPRAHHR	AHYETEGSRG	AVKAAPGGHP
460	470	480	490	500
VVKLLGYSEK	PLTLQMFIGT	ADERNLRPHA	FYQVHRITGK	MVATASYEAV
510	520	530	540	550
VSGTKVLEMT	LLPENNMAAN	IDCAGILKLR	NSDIELRKGE	TDIGRKNTRV
560	570	580	590	600
RLVFRVHVPQ	GGGKVVSVQA	ASVPIECSQR	SAQELPQVEA	YSPSACSVRG
610	620	630	640	650
GEELVLTGSN	FLPDSKVVFI	ERGPDGKLQW	EEEATVNRLQ	SNEVTLTLTV
660	670	680	690	700
PEYSNKRVSR	PVQVYFYVSN	GRRKRSPTQS	FRFLPVICKE	EPLPDSSLRG
710	720	730	740	750
FPSASATPFG	TDMDFSPPRP	PYPSYPHEDP	ACETPYLSEG	FGYGMPPLYP
760	770	780	790	800
QTGPPPSYRP	GLRMFPETRG	TTGCAQPPAV	SFLPRPFPSD	PYGGRGSSFS
810	820	830	840	850
LGLPFSPPAP	FRPPPLPASP	PLEGPFPSQS	DVHPLPAEGY	NKVGPGYGPG
860	870	880	890	900
EGAPEQEKSR	GGYSSGFRDS	VPIQGITLEE	VSEIIGRDLS	GFPAPPGEEP
902
PA

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt

Assay Details for CPTAC-1749 Collapse assay details

Data source: Panorama

Official Gene Symbol: NFATC4
Peptide Modified Sequence: T[+80.0]SSEQAVALPR
Modification Type: Phospho (ST)
Protein - Site of Modification: 333
Peptide - Site of Modification: 1
Peptide Start: 333
Peptide End: 343

CPTAC ID: CPTAC-1749
Peptide Molecular Mass: 1,237.5704
Species: Homo sapiens (Human)
Assay Type: Enrichment MRM or SRM
Enrichment Method: Titanium Dioxide
Matrix: Ovarian cancer cell lysate
Submitting Laboratory: Pacific Northwest National Laboratory
Submitting Lab PI: Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: TSQ Vantage
Internal Standard: peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: Waters nanoACQUITY (Part Number 176016000)
Column Packing: Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions: 100 um x 100 mm
Flow Rate: 0.5 uL/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-TiO2SRM

CPTAC-1724: STRN. DVDELPSLQPSVGSPS[+80.0]RPSSSR	CPTAC-1725: STRN. DVDELPSLQPSVGSPSRPSSS[+80.0]R	CPTAC-1726: PAK2. YLSFT[+80.0]PPEK
CPTAC-1727: SRF. ALIQTC[+57.0]LNSPDS[+80.0]PPR	CPTAC-1728: STAT5A. AVDGY[+80.0]VKPQIK	CPTAC-1729: DOCK7. LGTGFNPNT[+80.0]LDK
CPTAC-1730: DOCK7. MNS[+80.0]LTFK	CPTAC-1731: DOCK7. SPS[+80.0]GSAFGSQENLR	CPTAC-1732: DOCK7. AVLPVT[+80.0]C[+57.0]HR
CPTAC-1733: USP8. C[+57.0]PT[+80.0]PEIQK	CPTAC-1734: USP8. SYSS[+80.0]PDITQAIQEEEK	CPTAC-1735: ARHGEF6. KPS[+80.0]EEEYVIR
CPTAC-1736: ARHGEF6. DS[+80.0]IPQVLLPEEEK	CPTAC-1737: STRN. S[+80.0]VIDTSTIVR	CPTAC-1738: PLCG1. GSDAS[+80.0]GQLFHGR
CPTAC-1739: NF2. LT[+80.0]LQSAK	CPTAC-1740: PIK3R1. LY[+80.0]EEYTR	CPTAC-1741: CABLES1. QS[+80.0]TGAVSLK
CPTAC-1742: CABLES1. NTIDSTSS[+80.0]FSQFR	CPTAC-1743: PRKCD. SDSASS[+80.0]EPVGIYQGFEK	CPTAC-1744: NOC2L. SPDKPGGS[+80.0]PSASR
CPTAC-1745: CTNNB1. S[+80.0]PQMVSAIVR	CPTAC-1746: STAT1. TELIS[+80.0]VSEVHPSR	CPTAC-1747: RALBP1. TPS[+80.0]SEEISPTK
CPTAC-1748: RALBP1. TPSSEEIS[+80.0]PTK	CPTAC-1749: NFATC4. T[+80.0]SSEQAVALPR	CPTAC-1750: CTNNA1. T[+80.0]SVQTEDDQLIAGQSAR
CPTAC-1751: STAT3. YC[+57.0]RPESQEHPEADPGSAAPY[+80.0]LK

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y3 (1+)	20	6	7.7	19.2	11.1	8.8	27.7	12.6	11.7	15	15	15
y4 (1+)	12.1	3.7	6.9	12.6	5.8	7.1	17.5	6.9	9.9	15	15	15
y5 (1+)	14	5.7	8.4	15.7	5.6	9.5	21	8	12.7	15	15	15
sum	5.3	2.3	4.1	7.5	5.3	4.8	9.2	5.8	6.3	15	15	15

NFATC4

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-1749 Collapse assay details

Assay Parameters Collapse assay parameters

Assay Multiplexing Expand assay panel

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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NFATC4

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-1749 Collapse assay details

Assay Parameters Collapse assay parameters

Assay Multiplexing Expand assay panel

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments