RALBP1, CPTAC-1747 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
RALBP1	RLIP1, RLIP76

Sequence Length (AA)	Molecular Weight (Da)
655	76063

Protein Name
RalA-binding protein 1

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MTECFLPPTS	SPSEHRRVEH	GSGLTRTPSS	EEISPTKFPG	LYRTGEPSPP
60	70	80	90	100
HDILHEPPDV	VSDDEKDHGK	KKGKFKKKEK	RTEGYAAFQE	DSSGDEAESP
110	120	130	140	150
SKMKRSKGIH	VFKKPSFSKK	KEKDFKIKEK	PKEEKHKEEK	HKEEKHKEKK
160	170	180	190	200
SKDLTAADVV	KQWKEKKKKK	KPIQEPEVPQ	IDVPNLKPIF	GIPLADAVER
210	220	230	240	250
TMMYDGIRLP	AVFRECIDYV	EKYGMKCEGI	YRVSGIKSKV	DELKAAYDRE
260	270	280	290	300
ESTNLEDYEP	NTVASLLKQY	LRDLPENLLT	KELMPRFEEA	CGRTTETEKV
310	320	330	340	350
QEFQRLLKEL	PECNYLLISW	LIVHMDHVIA	KELETKMNIQ	NISIVLSPTV
360	370	380	390	400
QISNRVLYVF	FTHVQELFGN	VVLKQVMKPL	RWSNMATMPT	LPETQAGIKE
410	420	430	440	450
EIRRQEFLLN	CLHRDLQGGI	KDLSKEERLW	EVQRILTALK	RKLREAKRQE
460	470	480	490	500
CETKIAQEIA	SLSKEDVSKE	EMNENEEVIN	ILLAQENEIL	TEQEELLAME
510	520	530	540	550
QFLRRQIASE	KEEIERLRAE	IAEIQSRQQH	GRSETEEYSS	ESESESEDEE
560	570	580	590	600
ELQIILEDLQ	RQNEELEIKN	NHLNQAIHEE	REAIIELRVQ	LRLLQMQRAK
610	620	630	640	650
AEQQAQEDEE	PEWRGGAVQP	PRDGVLEPKA	AKEQPKAGKE	PAKPSPSRDR
655
KETSI

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-1747 Collapse assay details

Data source: Panorama

Official Gene Symbol: RALBP1
Peptide Modified Sequence: TPS[+80.0]SEEISPTK
Modification Type: Phospho (ST)
Protein - Site of Modification: 29
Peptide - Site of Modification: 3
Peptide Start: 27
Peptide End: 37

CPTAC ID: CPTAC-1747
Peptide Molecular Mass: 1,254.5381
Species: Homo sapiens (Human)
Assay Type: Enrichment MRM or SRM
Enrichment Method: Titanium Dioxide
Matrix: Ovarian cancer cell lysate
Submitting Laboratory: Pacific Northwest National Laboratory
Submitting Lab PI: Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: TSQ Vantage
Internal Standard: peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Waters nanoACQUITY (Part Number 176016000)
Column Packing: Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions: 100 um x 100 mm
Flow Rate: 0.5 uL/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-TiO2SRM

CPTAC-1724: STRN. DVDELPSLQPSVGSPS[+80.0]RPSSSR	CPTAC-1725: STRN. DVDELPSLQPSVGSPSRPSSS[+80.0]R	CPTAC-1726: PAK2. YLSFT[+80.0]PPEK
CPTAC-1727: SRF. ALIQTC[+57.0]LNSPDS[+80.0]PPR	CPTAC-1728: STAT5A. AVDGY[+80.0]VKPQIK	CPTAC-1729: DOCK7. LGTGFNPNT[+80.0]LDK
CPTAC-1730: DOCK7. MNS[+80.0]LTFK	CPTAC-1731: DOCK7. SPS[+80.0]GSAFGSQENLR	CPTAC-1732: DOCK7. AVLPVT[+80.0]C[+57.0]HR
CPTAC-1733: USP8. C[+57.0]PT[+80.0]PEIQK	CPTAC-1734: USP8. SYSS[+80.0]PDITQAIQEEEK	CPTAC-1735: ARHGEF6. KPS[+80.0]EEEYVIR
CPTAC-1736: ARHGEF6. DS[+80.0]IPQVLLPEEEK	CPTAC-1737: STRN. S[+80.0]VIDTSTIVR	CPTAC-1738: PLCG1. GSDAS[+80.0]GQLFHGR
CPTAC-1739: NF2. LT[+80.0]LQSAK	CPTAC-1740: PIK3R1. LY[+80.0]EEYTR	CPTAC-1741: CABLES1. QS[+80.0]TGAVSLK
CPTAC-1742: CABLES1. NTIDSTSS[+80.0]FSQFR	CPTAC-1743: PRKCD. SDSASS[+80.0]EPVGIYQGFEK	CPTAC-1744: NOC2L. SPDKPGGS[+80.0]PSASR
CPTAC-1745: CTNNB1. S[+80.0]PQMVSAIVR	CPTAC-1746: STAT1. TELIS[+80.0]VSEVHPSR	CPTAC-1747: RALBP1. TPS[+80.0]SEEISPTK
CPTAC-1748: RALBP1. TPSSEEIS[+80.0]PTK	CPTAC-1749: NFATC4. T[+80.0]SSEQAVALPR	CPTAC-1750: CTNNA1. T[+80.0]SVQTEDDQLIAGQSAR
CPTAC-1751: STAT3. YC[+57.0]RPESQEHPEADPGSAAPY[+80.0]LK

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y4 (1+)	9.5	5.5	8.9	11.9	5.8	8.1	15.2	8	12	15	15	15
y6 (1+)	19.5	8.3	9.3	16.9	8.5	9.7	25.8	11.9	13.4	15	15	15
y8 (1+)	16.7	13.9	16.1	23.2	14.8	20	28.6	20.3	25.7	15	15	15
sum	7.9	4.1	5.7	10.6	4.5	5.7	13.2	6.1	8.1	15	15	15

Additional Resources and Comments

Resources

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View Peptide in Panorama

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Comments

Assay Details for CPTAC-1748 Collapse assay details

Data source: Panorama

Official Gene Symbol: RALBP1
Peptide Modified Sequence: TPSSEEIS[+80.0]PTK
Modification Type: Phospho (ST)
Protein - Site of Modification: 34
Peptide - Site of Modification: 8
Peptide Start: 27
Peptide End: 37

CPTAC ID: CPTAC-1748
Peptide Molecular Mass: 1,254.5381
Species: Homo sapiens (Human)
Assay Type: Enrichment MRM or SRM
Enrichment Method: Titanium Dioxide
Matrix: Ovarian cancer cell lysate
Submitting Laboratory: Pacific Northwest National Laboratory
Submitting Lab PI: Tao Liu