PLCG1, CPTAC-1738 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

Please include the following statement when referencing the CPTAC Assay Portal

We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
PLCG1	PLC1

Sequence Length (AA)	Molecular Weight (Da)
1290	148532

Protein Name
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MAGAASPCAN	GCGPGAPSDA	EVLHLCRSLE	VGTVMTLFYS	KKSQRPERKT
60	70	80	90	100
FQVKLETRQI	TWSRGADKIE	GAIDIREIKE	IRPGKTSRDF	DRYQEDPAFR
110	120	130	140	150
PDQSHCFVIL	YGMEFRLKTL	SLQATSEDEV	NMWIKGLTWL	MEDTLQAPTP
160	170	180	190	200
LQIERWLRKQ	FYSVDRNRED	RISAKDLKNM	LSQVNYRVPN	MRFLRERLTD
210	220	230	240	250
LEQRSGDITY	GQFAQLYRSL	MYSAQKTMDL	PFLEASTLRA	GERPELCRVS
260	270	280	290	300
LPEFQQFLLD	YQGELWAVDR	LQVQEFMLSF	LRDPLREIEE	PYFFLDEFVT
310	320	330	340	350
FLFSKENSVW	NSQLDAVCPD	TMNNPLSHYW	ISSSHNTYLT	GDQFSSESSL
360	370	380	390	400
EAYARCLRMG	CRCIELDCWD	GPDGMPVIYH	GHTLTTKIKF	SDVLHTIKEH
410	420	430	440	450
AFVASEYPVI	LSIEDHCSIA	QQRNMAQYFK	KVLGDTLLTK	PVEISADGLP
460	470	480	490	500
SPNQLKRKIL	IKHKKLAEGS	AYEEVPTSMM	YSENDISNSI	KNGILYLEDP
510	520	530	540	550
VNHEWYPHYF	VLTSSKIYYS	EETSSDQGNE	DEEEPKEVSS	STELHSNEKW
560	570	580	590	600
FHGKLGAGRD	GRHIAERLLT	EYCIETGAPD	GSFLVRESET	FVGDYTLSFW
610	620	630	640	650
RNGKVQHCRI	HSRQDAGTPK	FFLTDNLVFD	SLYDLITHYQ	QVPLRCNEFE
660	670	680	690	700
MRLSEPVPQT	NAHESKEWYH	ASLTRAQAEH	MLMRVPRDGA	FLVRKRNEPN
710	720	730	740	750
SYAISFRAEG	KIKHCRVQQE	GQTVMLGNSE	FDSLVDLISY	YEKHPLYRKM
760	770	780	790	800
KLRYPINEEA	LEKIGTAEPD	YGALYEGRNP	GFYVEANPMP	TFKCAVKALF
810	820	830	840	850
DYKAQREDEL	TFIKSAIIQN	VEKQEGGWWR	GDYGGKKQLW	FPSNYVEEMV
860	870	880	890	900
NPVALEPERE	HLDENSPLGD	LLRGVLDVPA	CQIAIRPEGK	NNRLFVFSIS
910	920	930	940	950
MASVAHWSLD	VAADSQEELQ	DWVKKIREVA	QTADARLTEG	KIMERRKKIA
960	970	980	990	1000
LELSELVVYC	RPVPFDEEKI	GTERACYRDM	SSFPETKAEK	YVNKAKGKKF
1010	1020	1030	1040	1050
LQYNRLQLSR	IYPKGQRLDS	SNYDPLPMWI	CGSQLVALNF	QTPDKPMQMN
1060	1070	1080	1090	1100
QALFMTGRHC	GYVLQPSTMR	DEAFDPFDKS	SLRGLEPCAI	SIEVLGARHL
1110	1120	1130	1140	1150
PKNGRGIVCP	FVEIEVAGAE	YDSTKQKTEF	VVDNGLNPVW	PAKPFHFQIS
1160	1170	1180	1190	1200
NPEFAFLRFV	VYEEDMFSDQ	NFLAQATFPV	KGLKTGYRAV	PLKNNYSEDL
1210	1220	1230	1240	1250
ELASLLIKID	IFPAKENGDL	SPFSGTSLRE	RGSDASGQLF	HGRAREGSFE
1260	1270	1280	1290
SRYQQPFEDF	RISQEHLADH	FDSRERRAPR	RTRVNGDNRL

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below

All other points link out to UniProt

Assay Details for CPTAC-1738 Collapse assay details

Data source: Panorama

Official Gene Symbol: PLCG1
Peptide Modified Sequence: GSDAS[+80.0]GQLFHGR
Modification Type: Phospho (ST)
Protein - Site of Modification: 1236
Peptide - Site of Modification: 5
Peptide Start: 1232
Peptide End: 1243

CPTAC ID: CPTAC-1738
Peptide Molecular Mass: 1,310.5405
Species: Homo sapiens (Human)
Assay Type: Enrichment MRM or SRM
Enrichment Method: Titanium Dioxide
Matrix: Ovarian cancer cell lysate
Submitting Laboratory: Pacific Northwest National Laboratory
Submitting Lab PI: Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: TSQ Vantage
Internal Standard: peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: Waters nanoACQUITY (Part Number 176016000)
Column Packing: Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions: 100 um x 100 mm
Flow Rate: 0.5 uL/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-TiO2SRM

CPTAC-1724: STRN. DVDELPSLQPSVGSPS[+80.0]RPSSSR	CPTAC-1725: STRN. DVDELPSLQPSVGSPSRPSSS[+80.0]R	CPTAC-1726: PAK2. YLSFT[+80.0]PPEK
CPTAC-1727: SRF. ALIQTC[+57.0]LNSPDS[+80.0]PPR	CPTAC-1728: STAT5A. AVDGY[+80.0]VKPQIK	CPTAC-1729: DOCK7. LGTGFNPNT[+80.0]LDK
CPTAC-1730: DOCK7. MNS[+80.0]LTFK	CPTAC-1731: DOCK7. SPS[+80.0]GSAFGSQENLR	CPTAC-1732: DOCK7. AVLPVT[+80.0]C[+57.0]HR
CPTAC-1733: USP8. C[+57.0]PT[+80.0]PEIQK	CPTAC-1734: USP8. SYSS[+80.0]PDITQAIQEEEK	CPTAC-1735: ARHGEF6. KPS[+80.0]EEEYVIR
CPTAC-1736: ARHGEF6. DS[+80.0]IPQVLLPEEEK	CPTAC-1737: STRN. S[+80.0]VIDTSTIVR	CPTAC-1738: PLCG1. GSDAS[+80.0]GQLFHGR
CPTAC-1739: NF2. LT[+80.0]LQSAK	CPTAC-1740: PIK3R1. LY[+80.0]EEYTR	CPTAC-1741: CABLES1. QS[+80.0]TGAVSLK
CPTAC-1742: CABLES1. NTIDSTSS[+80.0]FSQFR	CPTAC-1743: PRKCD. SDSASS[+80.0]EPVGIYQGFEK	CPTAC-1744: NOC2L. SPDKPGGS[+80.0]PSASR
CPTAC-1745: CTNNB1. S[+80.0]PQMVSAIVR	CPTAC-1746: STAT1. TELIS[+80.0]VSEVHPSR	CPTAC-1747: RALBP1. TPS[+80.0]SEEISPTK
CPTAC-1748: RALBP1. TPSSEEIS[+80.0]PTK	CPTAC-1749: NFATC4. T[+80.0]SSEQAVALPR	CPTAC-1750: CTNNA1. T[+80.0]SVQTEDDQLIAGQSAR
CPTAC-1751: STAT3. YC[+57.0]RPESQEHPEADPGSAAPY[+80.0]LK

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y3 (1+)	9	6.6	7.5	12.4	7.9	6.3	15.3	10.3	9.8	15	15	15
y4 (1+)	19	7.9	7.9	16.9	9.5	8.6	25.4	12.4	11.7	15	15	15
y7 (1+)	12.9	8.7	4.9	19.7	9.1	5.9	23.5	12.6	7.7	15	15	15
sum	8.8	4.8	4.7	10.8	6.2	4.4	13.9	7.8	6.4	15	15	15

PLCG1

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-1738 Collapse assay details

Assay Parameters Collapse assay parameters

Assay Multiplexing Expand assay panel

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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PLCG1

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-1738 Collapse assay details

Assay Parameters Collapse assay parameters

Assay Multiplexing Expand assay panel

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments