CTNNA1, CPTAC-1750 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
CTNNA1	N/A

Sequence Length (AA)	Molecular Weight (Da)
906	100071

Protein Name
Catenin alpha-1

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MTAVHAGNIN	FKWDPKSLEI	RTLAVERLLE	PLVTQVTTLV	NTNSKGPSNK
60	70	80	90	100
KRGRSKKAHV	LAASVEQATE	NFLEKGDKIA	KESQFLKEEL	VAAVEDVRKQ
110	120	130	140	150
GDLMKAAAGE	FADDPCSSVK	RGNMVRAARA	LLSAVTRLLI	LADMADVYKL
160	170	180	190	200
LVQLKVVEDG	ILKLRNAGNE	QDLGIQYKAL	KPEVDKLNIM	AAKRQQELKD
210	220	230	240	250
VGHRDQMAAA	RGILQKNVPI	LYTASQACLQ	HPDVAAYKAN	RDLIYKQLQQ
260	270	280	290	300
AVTGISNAAQ	ATASDDASQH	QGGGGGELAY	ALNNFDKQII	VDPLSFSEER
310	320	330	340	350
FRPSLEERLE	SIISGAALMA	DSSCTRDDRR	ERIVAECNAV	RQALQDLLSE
360	370	380	390	400
YMGNAGRKER	SDALNSAIDK	MTKKTRDLRR	QLRKAVMDHV	SDSFLETNVP
410	420	430	440	450
LLVLIEAAKN	GNEKEVKEYA	QVFREHANKL	IEVANLACSI	SNNEEGVKLV
460	470	480	490	500
RMSASQLEAL	CPQVINAALA	LAAKPQSKLA	QENMDLFKEQ	WEKQVRVLTD
510	520	530	540	550
AVDDITSIDD	FLAVSENHIL	EDVNKCVIAL	QEKDVDGLDR	TAGAIRGRAA
560	570	580	590	600
RVIHVVTSEM	DNYEPGVYTE	KVLEATKLLS	NTVMPRFTEQ	VEAAVEALSS
610	620	630	640	650
DPAQPMDENE	FIDASRLVYD	GIRDIRKAVL	MIRTPEELDD	SDFETEDFDV
660	670	680	690	700
RSRTSVQTED	DQLIAGQSAR	AIMAQLPQEQ	KAKIAEQVAS	FQEEKSKLDA
710	720	730	740	750
EVSKWDDSGN	DIIVLAKQMC	MIMMEMTDFT	RGKGPLKNTS	DVISAAKKIA
760	770	780	790	800
EAGSRMDKLG	RTIADHCPDS	ACKQDLLAYL	QRIALYCHQL	NICSKVKAEV
810	820	830	840	850
QNLGGELVVS	GVDSAMSLIQ	AAKNLMNAVV	QTVKASYVAS	TKYQKSQGMA
860	870	880	890	900
SLNLPAVSWK	MKAPEKKPLV	KREKQDETQT	KIKRASQKKH	VNPVQALSEF
906
KAMDSI

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt

Assay Details for CPTAC-1750 Collapse assay details

Data source: Panorama

Official Gene Symbol: CTNNA1
Peptide Modified Sequence: T[+80.0]SVQTEDDQLIAGQSAR
Modification Type: Phospho (ST)
Protein - Site of Modification: 654
Peptide - Site of Modification: 1
Peptide Start: 654
Peptide End: 670

CPTAC ID: CPTAC-1750
Peptide Molecular Mass: 1,897.8419
Species: Homo sapiens (Human)
Assay Type: Enrichment MRM or SRM
Enrichment Method: Titanium Dioxide
Matrix: Ovarian cancer cell lysate
Submitting Laboratory: Pacific Northwest National Laboratory
Submitting Lab PI: Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: TSQ Vantage
Internal Standard: peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: Waters nanoACQUITY (Part Number 176016000)
Column Packing: Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions: 100 um x 100 mm
Flow Rate: 0.5 uL/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-TiO2SRM

CPTAC-1724: STRN. DVDELPSLQPSVGSPS[+80.0]RPSSSR	CPTAC-1725: STRN. DVDELPSLQPSVGSPSRPSSS[+80.0]R	CPTAC-1726: PAK2. YLSFT[+80.0]PPEK
CPTAC-1727: SRF. ALIQTC[+57.0]LNSPDS[+80.0]PPR	CPTAC-1728: STAT5A. AVDGY[+80.0]VKPQIK	CPTAC-1729: DOCK7. LGTGFNPNT[+80.0]LDK
CPTAC-1730: DOCK7. MNS[+80.0]LTFK	CPTAC-1731: DOCK7. SPS[+80.0]GSAFGSQENLR	CPTAC-1732: DOCK7. AVLPVT[+80.0]C[+57.0]HR
CPTAC-1733: USP8. C[+57.0]PT[+80.0]PEIQK	CPTAC-1734: USP8. SYSS[+80.0]PDITQAIQEEEK	CPTAC-1735: ARHGEF6. KPS[+80.0]EEEYVIR
CPTAC-1736: ARHGEF6. DS[+80.0]IPQVLLPEEEK	CPTAC-1737: STRN. S[+80.0]VIDTSTIVR	CPTAC-1738: PLCG1. GSDAS[+80.0]GQLFHGR
CPTAC-1739: NF2. LT[+80.0]LQSAK	CPTAC-1740: PIK3R1. LY[+80.0]EEYTR	CPTAC-1741: CABLES1. QS[+80.0]TGAVSLK
CPTAC-1742: CABLES1. NTIDSTSS[+80.0]FSQFR	CPTAC-1743: PRKCD. SDSASS[+80.0]EPVGIYQGFEK	CPTAC-1744: NOC2L. SPDKPGGS[+80.0]PSASR
CPTAC-1745: CTNNB1. S[+80.0]PQMVSAIVR	CPTAC-1746: STAT1. TELIS[+80.0]VSEVHPSR	CPTAC-1747: RALBP1. TPS[+80.0]SEEISPTK
CPTAC-1748: RALBP1. TPSSEEIS[+80.0]PTK	CPTAC-1749: NFATC4. T[+80.0]SSEQAVALPR	CPTAC-1750: CTNNA1. T[+80.0]SVQTEDDQLIAGQSAR
CPTAC-1751: STAT3. YC[+57.0]RPESQEHPEADPGSAAPY[+80.0]LK

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y3 (1+)	12.7	12.2	7.3	13.4	12.2	11.7	18.5	17.3	13.8	15	15	15
y6 (1+)	10.5	6.4	5.3	8.8	7	6.1	13.7	9.5	8.1	15	15	15
y7 (1+)	11.2	7.9	10.8	13.2	8.1	12	17.3	11.3	16.1	15	15	15
sum	7	4.1	4.9	6.8	5.5	6.7	9.8	6.9	8.3	15	15	15

CTNNA1

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-1750 Collapse assay details

Assay Parameters Collapse assay parameters

Assay Multiplexing Expand assay panel

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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CTNNA1

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-1750 Collapse assay details

Assay Parameters Collapse assay parameters

Assay Multiplexing Expand assay panel

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments