NF2, CPTAC-1739 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
NF2	SCH

Sequence Length (AA)	Molecular Weight (Da)
595	69690

Protein Name
Merlin

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MAGAIASRMS	FSSLKRKQPK	TFTVRIVTMD	AEMEFNCEMK	WKGKDLFDLV
60	70	80	90	100
CRTLGLRETW	FFGLQYTIKD	TVAWLKMDKK	VLDHDVSKEE	PVTFHFLAKF
110	120	130	140	150
YPENAEEELV	QEITQHLFFL	QVKKQILDEK	IYCPPEASVL	LASYAVQAKY
160	170	180	190	200
GDYDPSVHKR	GFLAQEELLP	KRVINLYQMT	PEMWEERITA	WYAEHRGRAR
210	220	230	240	250
DEAEMEYLKI	AQDLEMYGVN	YFAIRNKKGT	ELLLGVDALG	LHIYDPENRL
260	270	280	290	300
TPKISFPWNE	IRNISYSDKE	FTIKPLDKKI	DVFKFNSSKL	RVNKLILQLC
310	320	330	340	350
IGNHDLFMRR	RKADSLEVQQ	MKAQAREEKA	RKQMERQRLA	REKQMREEAE
360	370	380	390	400
RTRDELERRL	LQMKEEATMA	NEALMRSEET	ADLLAEKAQI	TEEEAKLLAQ
410	420	430	440	450
KAAEAEQEMQ	RIKATAIRTE	EEKRLMEQKV	LEAEVLALKM	AEESERRAKE
460	470	480	490	500
ADQLKQDLQE	AREAERRAKQ	KLLEIATKPT	YPPMNPIPAP	LPPDIPSFNL
510	520	530	540	550
IGDSLSFDFK	DTDMKRLSME	IEKEKVEYME	KSKHLQEQLN	ELKTEIEALK
560	570	580	590	595
LKERETALDI	LHNENSDRGG	SSKHNTIKKL	TLQSAKSRVA	FFEEL

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt

Assay Details for CPTAC-1739 Collapse assay details

Data source: Panorama

Official Gene Symbol: NF2
Peptide Modified Sequence: LT[+80.0]LQSAK
Modification Type: Phospho (ST)
Protein - Site of Modification: 581
Peptide - Site of Modification: 2
Peptide Start: 580
Peptide End: 586

CPTAC ID: CPTAC-1739
Peptide Molecular Mass: 839.4154
Species: Homo sapiens (Human)
Assay Type: Enrichment MRM or SRM
Enrichment Method: Titanium Dioxide
Matrix: Ovarian cancer cell lysate
Submitting Laboratory: Pacific Northwest National Laboratory
Submitting Lab PI: Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: TSQ Vantage
Internal Standard: peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Waters nanoACQUITY (Part Number 176016000)
Column Packing: Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions: 100 um x 100 mm
Flow Rate: 0.5 uL/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-TiO2SRM

CPTAC-1724: STRN. DVDELPSLQPSVGSPS[+80.0]RPSSSR	CPTAC-1725: STRN. DVDELPSLQPSVGSPSRPSSS[+80.0]R	CPTAC-1726: PAK2. YLSFT[+80.0]PPEK
CPTAC-1727: SRF. ALIQTC[+57.0]LNSPDS[+80.0]PPR	CPTAC-1728: STAT5A. AVDGY[+80.0]VKPQIK	CPTAC-1729: DOCK7. LGTGFNPNT[+80.0]LDK
CPTAC-1730: DOCK7. MNS[+80.0]LTFK	CPTAC-1731: DOCK7. SPS[+80.0]GSAFGSQENLR	CPTAC-1732: DOCK7. AVLPVT[+80.0]C[+57.0]HR
CPTAC-1733: USP8. C[+57.0]PT[+80.0]PEIQK	CPTAC-1734: USP8. SYSS[+80.0]PDITQAIQEEEK	CPTAC-1735: ARHGEF6. KPS[+80.0]EEEYVIR
CPTAC-1736: ARHGEF6. DS[+80.0]IPQVLLPEEEK	CPTAC-1737: STRN. S[+80.0]VIDTSTIVR	CPTAC-1738: PLCG1. GSDAS[+80.0]GQLFHGR
CPTAC-1739: NF2. LT[+80.0]LQSAK	CPTAC-1740: PIK3R1. LY[+80.0]EEYTR	CPTAC-1741: CABLES1. QS[+80.0]TGAVSLK
CPTAC-1742: CABLES1. NTIDSTSS[+80.0]FSQFR	CPTAC-1743: PRKCD. SDSASS[+80.0]EPVGIYQGFEK	CPTAC-1744: NOC2L. SPDKPGGS[+80.0]PSASR
CPTAC-1745: CTNNB1. S[+80.0]PQMVSAIVR	CPTAC-1746: STAT1. TELIS[+80.0]VSEVHPSR	CPTAC-1747: RALBP1. TPS[+80.0]SEEISPTK
CPTAC-1748: RALBP1. TPSSEEIS[+80.0]PTK	CPTAC-1749: NFATC4. T[+80.0]SSEQAVALPR	CPTAC-1750: CTNNA1. T[+80.0]SVQTEDDQLIAGQSAR
CPTAC-1751: STAT3. YC[+57.0]RPESQEHPEADPGSAAPY[+80.0]LK

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y4 (1+)	15.4	7	12.6	13.7	9	13.5	20.6	11.4	18.5	15	15	15
y5 (1+)	4.1	5.5	6.2	7.6	6.9	7.7	8.6	8.8	9.9	15	15	15
y6 (1+)	25.3	7.7	11.7	36.6	10.9	14.7	44.5	13.3	18.8	15	15	15
sum	5.9	3	6.2	8.1	3.5	6.6	10	4.6	9.1	15	15	15

NF2

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-1739 Collapse assay details

Assay Parameters Collapse assay parameters

Assay Multiplexing Expand assay panel

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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NF2

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-1739 Collapse assay details

Assay Parameters Collapse assay parameters

Assay Multiplexing Expand assay panel

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments