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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
PRKCD N/A
Sequence Length (AA) Molecular Weight (Da)
676 77505
Protein Name
Protein kinase C delta type
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MAPFLRIAFN SYELGSLQAE DEANQPFCAV KMKEALSTER GKTLVQKKPT
60 70 80 90 100
MYPEWKSTFD AHIYEGRVIQ IVLMRAAEEP VSEVTVGVSV LAERCKKNNG
110 120 130 140 150
KAEFWLDLQP QAKVLMSVQY FLEDVDCKQS MRSEDEAKFP TMNRRGAIKQ
160 170 180 190 200
AKIHYIKNHE FIATFFGQPT FCSVCKDFVW GLNKQGYKCR QCNAAIHKKC
210 220 230 240 250
IDKIIGRCTG TAANSRDTIF QKERFNIDMP HRFKVHNYMS PTFCDHCGSL
260 270 280 290 300
LWGLVKQGLK CEDCGMNVHH KCREKVANLC GINQKLLAEA LNQVTQRASR
310 320 330 340 350
RSDSASSEPV GIYQGFEKKT GVAGEDMQDN SGTYGKIWEG SSKCNINNFI
360 370 380 390 400
FHKVLGKGSF GKVLLGELKG RGEYFAIKAL KKDVVLIDDD VECTMVEKRV
410 420 430 440 450
LTLAAENPFL THLICTFQTK DHLFFVMEFL NGGDLMYHIQ DKGRFELYRA
460 470 480 490 500
TFYAAEIMCG LQFLHSKGII YRDLKLDNVL LDRDGHIKIA DFGMCKENIF
510 520 530 540 550
GESRASTFCG TPDYIAPEIL QGLKYTFSVD WWSFGVLLYE MLIGQSPFHG
560 570 580 590 600
DDEDELFESI RVDTPHYPRW ITKESKDILE KLFEREPTKR LGVTGNIKIH
610 620 630 640 650
PFFKTINWTL LEKRRLEPPF RPKVKSPRDY SNFDQEFLNE KARLSYSDKN
660 670 676
LIDSMDQSAF AGFSFVNPKF EHLLED

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1743 Collapse assay details

Data source: Panorama

Official Gene Symbol
PRKCD
Peptide Modified Sequence
SDSASS[+80.0]EPVGIYQGFEK
Modification Type
Phospho (ST)
Protein - Site of Modification
307
Peptide - Site of Modification
6
Peptide Start
302
Peptide End
318
CPTAC ID
CPTAC-1743
Peptide Molecular Mass
1,879.7877
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM or SRM
Enrichment Method
Titanium Dioxide
Matrix
Ovarian cancer cell lysate
Submitting Laboratory
Pacific Northwest National Laboratory
Submitting Lab PI
Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Waters nanoACQUITY (Part Number 176016000)
Column Packing
Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions
100 um x 100 mm
Flow Rate
0.5 uL/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-TiO2SRM


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y4 (1+) 13.4 7.6 7 17.2 10.8 10.5 21.8 13.2 12.6 15 15 15
y8 (1+) 38.8 27.1 15.1 30 33.3 18.3 49 42.9 23.7 15 15 15
y6 (1+) 8 7 6.2 11.4 9.5 6.6 13.9 11.8 9.1 15 15 15
sum 14 7.4 5.8 12.6 11.7 7.4 18.8 13.8 9.4 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-2854 Collapse assay details

Data source: Panorama

Official Gene Symbol
PRKCD
Peptide Sequence
AEFWLDLQPQAK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
102
Peptide End
113
CPTAC ID
CPTAC-2854
Peptide Molecular Mass
1,444.7351
Species
Homo Sapiens
Assay Type
Direct MRM or SRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
ThermoFisher, Q-Exactive
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Easy-nLC 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nL/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-TiO2SRM


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 4.9 5.7 4.5 5.3 7.6 6.4 7.2 9.5 7.8 15 15 15
y4 (1+) 4.2 4.6 3.6 4.7 6.5 5.5 6.3 8 6.6 15 15 15
y8 (1+) 4.4 6.2 4.2 5.7 8 6.9 7.2 10.1 8.1 15 15 15
sum 4.1 5.1 3.8 4.8 7 5.9 6.3 8.7 7 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-2853 Collapse assay details

Data source: Panorama

Official Gene Symbol
PRKCD
Peptide Sequence
DYSNFDQEFLNEK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
629
Peptide End
641
CPTAC ID
CPTAC-2853
Peptide Molecular Mass
1,647.7053
Species
Homo Sapiens
Assay Type
Direct MRM or SRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
ThermoFisher, Q-Exactive
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Easy-nLC 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nL/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-TiO2SRM


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (1+) 5.7 4.4 8 9.6 5.8 5.9 11.2 7.3 9.9 15 15 15
y3 (1+) 6.7 5 3.6 9.2 4.8 3.8 11.4 6.9 5.2 15 15 15
y4 (1+) 8.5 4.1 4.4 10.9 4.2 4.5 13.8 5.9 6.3 15 15 15
sum 5.2 4.4 5.1 7.8 4.6 4.5 9.4 6.4 6.8 15 15 15


Additional Resources and Comments