Show Nav →

Overview Data source: UniProt

Official Gene Symbol Other Aliases
SRF N/A
Sequence Length (AA) Molecular Weight (Da)
508 51593
Protein Name
Serum response factor
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MLPTQAGAAA ALGRGSALGG SLNRTPTGRP GGGGGTRGAN GGRVPGNGAG
60 70 80 90 100
LGPGRLEREA AAAAATTPAP TAGALYSGSE GDSESGEEEE LGAERRGLKR
110 120 130 140 150
SLSEMEIGMV VGGPEASAAA TGGYGPVSGA VSGAKPGKKT RGRVKIKMEF
160 170 180 190 200
IDNKLRRYTT FSKRKTGIMK KAYELSTLTG TQVLLLVASE TGHVYTFATR
210 220 230 240 250
KLQPMITSET GKALIQTCLN SPDSPPRSDP TTDQRMSATG FEETDLTYQV
260 270 280 290 300
SESDSSGETK DTLKPAFTVT NLPGTTSTIQ TAPSTSTTMQ VSSGPSFPIT
310 320 330 340 350
NYLAPVSASV SPSAVSSANG TVLKSTGSGP VSSGGLMQLP TSFTLMPGGA
360 370 380 390 400
VAQQVPVQAI QVHQAPQQAS PSRDSSTDLT QTSSSGTVTL PATIMTSSVP
410 420 430 440 450
TTVGGHMMYP SPHAVMYAPT SGLGDGSLTV LNAFSQAPST MQVSHSQVQE
460 470 480 490 500
PGGVPQVFLT ASSGTVQIPV SAVQLHQMAV IGQQAGSSSN LTELQVVNLD
508
TAHSTKSE

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node
to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

loading

Loader

Assay Details for CPTAC-1727 Collapse assay details

Data source: Panorama

Official Gene Symbol
SRF
Peptide Modified Sequence
ALIQTC[+57.0]LNSPDS[+80.0]PPR
Modification Type
Carbamidomethyl (C), Phospho (ST)
Protein - Site of Modification
218, 224
Peptide - Site of Modification
6, 12
Peptide Start
213
Peptide End
227
CPTAC ID
CPTAC-1727
Peptide Molecular Mass
1,747.7964
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM or SRM
Enrichment Method
Titanium Dioxide
Matrix
Ovarian cancer cell lysate
Submitting Laboratory
Pacific Northwest National Laboratory
Submitting Lab PI
Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Waters nanoACQUITY (Part Number 176016000)
Column Packing
Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions
100 um x 100 mm
Flow Rate
0.5 uL/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-TiO2SRM


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 6.9 5.5 3.2 12.2 6.5 7.1 14 8.5 7.8 15 15 15
y6 (1+) 14.1 5 3.4 14.8 4.8 5.4 20.4 6.9 6.4 15 15 15
y7 (1+) 13.9 7.1 3.8 13.8 4.2 6.8 19.6 8.2 7.8 15 15 15
sum 6.9 4.3 1.9 9 4.7 5.7 11.3 6.4 6 15 15 15

Additional Resources and Comments


Assay Details for CPTAC-1764 Collapse assay details

Data source: Panorama

Official Gene Symbol
SRF
Peptide Modified Sequence
ALIQTC[+57.0]LNSPDSPPR
Modification Type
Carbamidomethyl (C)
Protein - Site of Modification
218
Peptide - Site of Modification
6
Peptide Start
213
Peptide End
227
CPTAC ID
CPTAC-1764
Peptide Molecular Mass
1,667.8301
Species
Homo sapiens (Human)
Assay Type
Fractionation MRM or SRM
Fractionation Approach
N/A
Matrix
Ovarian cancer tumor tissue lysate
Submitting Laboratory
Pacific Northwest National Laboratory
Submitting Lab PI
Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Waters nanoACQUITY (Part Number 176016000)
Column Packing
Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions
100 um x 100 mm
Flow Rate
0.4 (uL/min)

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-TiO2SRM


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y4 (1+) 4.4 3.7 3.9 5.8 5.4 5.6 7.3 6.5 6.8 15 15 15
y6 (1+) 3.8 3.8 4.1 5.1 3.6 3.6 6.4 5.2 5.5 15 15 15
y8 (1+) 2.5 3.6 6.7 6.4 3.5 7.3 6.9 5 9.9 15 15 15
sum 2.5 3.2 3.1 5 3.4 3.1 5.6 4.7 4.4 15 15 15

Additional Resources and Comments