SRF, CPTAC-1727 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
SRF	N/A

Sequence Length (AA)	Molecular Weight (Da)
508	51593

Protein Name
Serum response factor

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MLPTQAGAAA	ALGRGSALGG	SLNRTPTGRP	GGGGGTRGAN	GGRVPGNGAG
60	70	80	90	100
LGPGRLEREA	AAAAATTPAP	TAGALYSGSE	GDSESGEEEE	LGAERRGLKR
110	120	130	140	150
SLSEMEIGMV	VGGPEASAAA	TGGYGPVSGA	VSGAKPGKKT	RGRVKIKMEF
160	170	180	190	200
IDNKLRRYTT	FSKRKTGIMK	KAYELSTLTG	TQVLLLVASE	TGHVYTFATR
210	220	230	240	250
KLQPMITSET	GKALIQTCLN	SPDSPPRSDP	TTDQRMSATG	FEETDLTYQV
260	270	280	290	300
SESDSSGETK	DTLKPAFTVT	NLPGTTSTIQ	TAPSTSTTMQ	VSSGPSFPIT
310	320	330	340	350
NYLAPVSASV	SPSAVSSANG	TVLKSTGSGP	VSSGGLMQLP	TSFTLMPGGA
360	370	380	390	400
VAQQVPVQAI	QVHQAPQQAS	PSRDSSTDLT	QTSSSGTVTL	PATIMTSSVP
410	420	430	440	450
TTVGGHMMYP	SPHAVMYAPT	SGLGDGSLTV	LNAFSQAPST	MQVSHSQVQE
460	470	480	490	500
PGGVPQVFLT	ASSGTVQIPV	SAVQLHQMAV	IGQQAGSSSN	LTELQVVNLD
508
TAHSTKSE

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-1727 Collapse assay details

Data source: Panorama

Official Gene Symbol: SRF
Peptide Modified Sequence: ALIQTC[+57.0]LNSPDS[+80.0]PPR
Modification Type: Carbamidomethyl (C), Phospho (ST)
Protein - Site of Modification: 218, 224
Peptide - Site of Modification: 6, 12
Peptide Start: 213
Peptide End: 227

CPTAC ID: CPTAC-1727
Peptide Molecular Mass: 1,747.7964
Species: Homo sapiens (Human)
Assay Type: Enrichment MRM or SRM
Enrichment Method: Titanium Dioxide
Matrix: Ovarian cancer cell lysate
Submitting Laboratory: Pacific Northwest National Laboratory
Submitting Lab PI: Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: TSQ Vantage
Internal Standard: peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: Waters nanoACQUITY (Part Number 176016000)
Column Packing: Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions: 100 um x 100 mm
Flow Rate: 0.5 uL/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-TiO2SRM

CPTAC-1724: STRN. DVDELPSLQPSVGSPS[+80.0]RPSSSR	CPTAC-1725: STRN. DVDELPSLQPSVGSPSRPSSS[+80.0]R	CPTAC-1726: PAK2. YLSFT[+80.0]PPEK
CPTAC-1727: SRF. ALIQTC[+57.0]LNSPDS[+80.0]PPR	CPTAC-1728: STAT5A. AVDGY[+80.0]VKPQIK	CPTAC-1729: DOCK7. LGTGFNPNT[+80.0]LDK
CPTAC-1730: DOCK7. MNS[+80.0]LTFK	CPTAC-1731: DOCK7. SPS[+80.0]GSAFGSQENLR	CPTAC-1732: DOCK7. AVLPVT[+80.0]C[+57.0]HR
CPTAC-1733: USP8. C[+57.0]PT[+80.0]PEIQK	CPTAC-1734: USP8. SYSS[+80.0]PDITQAIQEEEK	CPTAC-1735: ARHGEF6. KPS[+80.0]EEEYVIR
CPTAC-1736: ARHGEF6. DS[+80.0]IPQVLLPEEEK	CPTAC-1737: STRN. S[+80.0]VIDTSTIVR	CPTAC-1738: PLCG1. GSDAS[+80.0]GQLFHGR
CPTAC-1739: NF2. LT[+80.0]LQSAK	CPTAC-1740: PIK3R1. LY[+80.0]EEYTR	CPTAC-1741: CABLES1. QS[+80.0]TGAVSLK
CPTAC-1742: CABLES1. NTIDSTSS[+80.0]FSQFR	CPTAC-1743: PRKCD. SDSASS[+80.0]EPVGIYQGFEK	CPTAC-1744: NOC2L. SPDKPGGS[+80.0]PSASR
CPTAC-1745: CTNNB1. S[+80.0]PQMVSAIVR	CPTAC-1746: STAT1. TELIS[+80.0]VSEVHPSR	CPTAC-1747: RALBP1. TPS[+80.0]SEEISPTK
CPTAC-1748: RALBP1. TPSSEEIS[+80.0]PTK	CPTAC-1749: NFATC4. T[+80.0]SSEQAVALPR	CPTAC-1750: CTNNA1. T[+80.0]SVQTEDDQLIAGQSAR
CPTAC-1751: STAT3. YC[+57.0]RPESQEHPEADPGSAAPY[+80.0]LK

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y3 (1+)	6.9	5.5	3.2	12.2	6.5	7.1	14	8.5	7.8	15	15	15
y6 (1+)	14.1	5	3.4	14.8	4.8	5.4	20.4	6.9	6.4	15	15	15
y7 (1+)	13.9	7.1	3.8	13.8	4.2	6.8	19.6	8.2	7.8	15	15	15
sum	6.9	4.3	1.9	9	4.7	5.7	11.3	6.4	6	15	15	15

Additional Resources and Comments

Resources

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View Peptide in Panorama

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Comments

Assay Details for CPTAC-1764 Collapse assay details

Data source: Panorama

Official Gene Symbol: SRF
Peptide Modified Sequence: ALIQTC[+57.0]LNSPDSPPR
Modification Type: Carbamidomethyl (C)
Protein - Site of Modification: 218
Peptide - Site of Modification: 6
Peptide Start: 213
Peptide End: 227

CPTAC ID: CPTAC-1764
Peptide Molecular Mass: 1,667.8301
Species: Homo sapiens (Human)
Assay Type: Fractionation MRM or SRM
Fractionation Approach: N/A
Matrix: Ovarian cancer tumor tissue lysate
Submitting Laboratory: Pacific Northwest National Laboratory
Submitting Lab PI: Tao Liu