USP8, CPTAC-1733 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
USP8	KIAA0055, UBPY

Sequence Length (AA)	Molecular Weight (Da)
1118	127523

Protein Name
Ubiquitin carboxyl-terminal hydrolase 8

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MPAVASVPKE	LYLSSSLKDL	NKKTEVKPEK	ISTKSYVHSA	LKIFKTAEEC
60	70	80	90	100
RLDRDEERAY	VLYMKYVTVY	NLIKKRPDFK	QQQDYFHSIL	GPGNIKKAVE
110	120	130	140	150
EAERLSESLK	LRYEEAEVRK	KLEEKDRQEE	AQRLQQKRQE	TGREDGGTLA
160	170	180	190	200
KGSLENVLDS	KDKTQKSNGE	KNEKCETKEK	GAITAKELYT	MMTDKNISLI
210	220	230	240	250
IMDARRMQDY	QDSCILHSLS	VPEEAISPGV	TASWIEAHLP	DDSKDTWKKR
260	270	280	290	300
GNVEYVVLLD	WFSSAKDLQI	GTTLRSLKDA	LFKWESKTVL	RNEPLVLEGG
310	320	330	340	350
YENWLLCYPQ	YTTNAKVTPP	PRRQNEEVSI	SLDFTYPSLE	ESIPSKPAAQ
360	370	380	390	400
TPPASIEVDE	NIELISGQNE	RMGPLNISTP	VEPVAASKSD	VSPIIQPVPS
410	420	430	440	450
IKNVPQIDRT	KKPAVKLPEE	HRIKSESTNH	EQQSPQSGKV	IPDRSTKPVV
460	470	480	490	500
FSPTLMLTDE	EKARIHAETA	LLMEKNKQEK	ELRERQQEEQ	KEKLRKEEQE
510	520	530	540	550
QKAKKKQEAE	ENEITEKQQK	AKEEMEKKES	EQAKKEDKET	SAKRGKEITG
560	570	580	590	600
VKRQSKSEHE	TSDAKKSVED	RGKRCPTPEI	QKKSTGDVPH	TSVTGDSGSG
610	620	630	640	650
KPFKIKGQPE	SGILRTGTFR	EDTDDTERNK	AQREPLTRAR	SEEMGRIVPG
660	670	680	690	700
LPSGWAKFLD	PITGTFRYYH	SPTNTVHMYP	PEMAPSSAPP	STPPTHKAKP
710	720	730	740	750
QIPAERDREP	SKLKRSYSSP	DITQAIQEEE	KRKPTVTPTV	NRENKPTCYP
760	770	780	790	800
KAEISRLSAS	QIRNLNPVFG	GSGPALTGLR	NLGNTCYMNS	ILQCLCNAPH
810	820	830	840	850
LADYFNRNCY	QDDINRSNLL	GHKGEVAEEF	GIIMKALWTG	QYRYISPKDF
860	870	880	890	900
KITIGKINDQ	FAGYSQQDSQ	ELLLFLMDGL	HEDLNKADNR	KRYKEENNDH
910	920	930	940	950
LDDFKAAEHA	WQKHKQLNES	IIVALFQGQF	KSTVQCLTCH	KKSRTFEAFM
960	970	980	990	1000
YLSLPLASTS	KCTLQDCLRL	FSKEEKLTDN	NRFYCSHCRA	RRDSLKKIEI
1010	1020	1030	1040	1050
WKLPPVLLVH	LKRFSYDGRW	KQKLQTSVDF	PLENLDLSQY	VIGPKNNLKK
1060	1070	1080	1090	1100
YNLFSVSNHY	GGLDGGHYTA	YCKNAARQRW	FKFDDHEVSD	ISVSSVKSSA
1110	1118
AYILFYTSLG	PRVTDVAT

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below

All other points link out to UniProt

Assay Details for CPTAC-1733 Collapse assay details

Data source: Panorama

Official Gene Symbol: USP8
Peptide Modified Sequence: C[+57.0]PT[+80.0]PEIQK
Modification Type: Carbamidomethyl (C), Phospho (ST)
Protein - Site of Modification: 575, 577
Peptide - Site of Modification: 1, 3
Peptide Start: 575
Peptide End: 582

CPTAC ID: CPTAC-1733
Peptide Molecular Mass: 1,051.4409
Species: Homo sapiens (Human)
Assay Type: Enrichment MRM or SRM
Enrichment Method: Titanium Dioxide
Matrix: Ovarian cancer cell lysate
Submitting Laboratory: Pacific Northwest National Laboratory
Submitting Lab PI: Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: TSQ Vantage
Internal Standard: peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Waters nanoACQUITY (Part Number 176016000)
Column Packing: Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions: 100 um x 100 mm
Flow Rate: 0.5 uL/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-TiO2SRM

CPTAC-1724: STRN. DVDELPSLQPSVGSPS[+80.0]RPSSSR	CPTAC-1725: STRN. DVDELPSLQPSVGSPSRPSSS[+80.0]R	CPTAC-1726: PAK2. YLSFT[+80.0]PPEK
CPTAC-1727: SRF. ALIQTC[+57.0]LNSPDS[+80.0]PPR	CPTAC-1728: STAT5A. AVDGY[+80.0]VKPQIK	CPTAC-1729: DOCK7. LGTGFNPNT[+80.0]LDK
CPTAC-1730: DOCK7. MNS[+80.0]LTFK	CPTAC-1731: DOCK7. SPS[+80.0]GSAFGSQENLR	CPTAC-1732: DOCK7. AVLPVT[+80.0]C[+57.0]HR
CPTAC-1733: USP8. C[+57.0]PT[+80.0]PEIQK	CPTAC-1734: USP8. SYSS[+80.0]PDITQAIQEEEK	CPTAC-1735: ARHGEF6. KPS[+80.0]EEEYVIR
CPTAC-1736: ARHGEF6. DS[+80.0]IPQVLLPEEEK	CPTAC-1737: STRN. S[+80.0]VIDTSTIVR	CPTAC-1738: PLCG1. GSDAS[+80.0]GQLFHGR
CPTAC-1739: NF2. LT[+80.0]LQSAK	CPTAC-1740: PIK3R1. LY[+80.0]EEYTR	CPTAC-1741: CABLES1. QS[+80.0]TGAVSLK
CPTAC-1742: CABLES1. NTIDSTSS[+80.0]FSQFR	CPTAC-1743: PRKCD. SDSASS[+80.0]EPVGIYQGFEK	CPTAC-1744: NOC2L. SPDKPGGS[+80.0]PSASR
CPTAC-1745: CTNNB1. S[+80.0]PQMVSAIVR	CPTAC-1746: STAT1. TELIS[+80.0]VSEVHPSR	CPTAC-1747: RALBP1. TPS[+80.0]SEEISPTK
CPTAC-1748: RALBP1. TPSSEEIS[+80.0]PTK	CPTAC-1749: NFATC4. T[+80.0]SSEQAVALPR	CPTAC-1750: CTNNA1. T[+80.0]SVQTEDDQLIAGQSAR
CPTAC-1751: STAT3. YC[+57.0]RPESQEHPEADPGSAAPY[+80.0]LK

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y7 (2+)	31.5	6.5	4.8	25.9	10.3	7.9	40.8	12.2	9.2	15	15	15
y3 (1+)	14.4	6.1	5.6	22.4	7.3	6	26.6	9.5	8.2	15	15	15
y5 (1+)	9.1	3	2.5	10.5	5.6	3.4	13.9	6.4	4.2	15	15	15
sum	10.8	2.3	1.3	11.3	4	3.2	15.6	4.6	3.5	15	15	15

Additional Resources and Comments

Resources

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View Peptide in Panorama

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Comments

Assay Details for CPTAC-1734 Collapse assay details

Data source: Panorama

Official Gene Symbol: USP8
Peptide Modified Sequence: SYSS[+80.0]PDITQAIQEEEK
Modification Type: Phospho (ST)
Protein - Site of Modification: 719
Peptide - Site of Modification: 4
Peptide Start: 716
Peptide End: 731

CPTAC ID: CPTAC-1734
Peptide Molecular Mass: 1,903.8088
Species: Homo sapiens (Human)
Assay Type: Enrichment MRM or SRM
Enrichment Method: Titanium Dioxide
Matrix: Ovarian cancer cell lysate
Submitting Laboratory: Pacific Northwest National Laboratory
Submitting Lab PI: Tao Liu