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Overview Data source: UniProt

Official Gene Symbol Other Aliases
USP8 KIAA0055, UBPY
Sequence Length (AA) Molecular Weight (Da)
1118 127523
Protein Name
Ubiquitin carboxyl-terminal hydrolase 8
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MPAVASVPKE LYLSSSLKDL NKKTEVKPEK ISTKSYVHSA LKIFKTAEEC
60 70 80 90 100
RLDRDEERAY VLYMKYVTVY NLIKKRPDFK QQQDYFHSIL GPGNIKKAVE
110 120 130 140 150
EAERLSESLK LRYEEAEVRK KLEEKDRQEE AQRLQQKRQE TGREDGGTLA
160 170 180 190 200
KGSLENVLDS KDKTQKSNGE KNEKCETKEK GAITAKELYT MMTDKNISLI
210 220 230 240 250
IMDARRMQDY QDSCILHSLS VPEEAISPGV TASWIEAHLP DDSKDTWKKR
260 270 280 290 300
GNVEYVVLLD WFSSAKDLQI GTTLRSLKDA LFKWESKTVL RNEPLVLEGG
310 320 330 340 350
YENWLLCYPQ YTTNAKVTPP PRRQNEEVSI SLDFTYPSLE ESIPSKPAAQ
360 370 380 390 400
TPPASIEVDE NIELISGQNE RMGPLNISTP VEPVAASKSD VSPIIQPVPS
410 420 430 440 450
IKNVPQIDRT KKPAVKLPEE HRIKSESTNH EQQSPQSGKV IPDRSTKPVV
460 470 480 490 500
FSPTLMLTDE EKARIHAETA LLMEKNKQEK ELRERQQEEQ KEKLRKEEQE
510 520 530 540 550
QKAKKKQEAE ENEITEKQQK AKEEMEKKES EQAKKEDKET SAKRGKEITG
560 570 580 590 600
VKRQSKSEHE TSDAKKSVED RGKRCPTPEI QKKSTGDVPH TSVTGDSGSG
610 620 630 640 650
KPFKIKGQPE SGILRTGTFR EDTDDTERNK AQREPLTRAR SEEMGRIVPG
660 670 680 690 700
LPSGWAKFLD PITGTFRYYH SPTNTVHMYP PEMAPSSAPP STPPTHKAKP
710 720 730 740 750
QIPAERDREP SKLKRSYSSP DITQAIQEEE KRKPTVTPTV NRENKPTCYP
760 770 780 790 800
KAEISRLSAS QIRNLNPVFG GSGPALTGLR NLGNTCYMNS ILQCLCNAPH
810 820 830 840 850
LADYFNRNCY QDDINRSNLL GHKGEVAEEF GIIMKALWTG QYRYISPKDF
860 870 880 890 900
KITIGKINDQ FAGYSQQDSQ ELLLFLMDGL HEDLNKADNR KRYKEENNDH
910 920 930 940 950
LDDFKAAEHA WQKHKQLNES IIVALFQGQF KSTVQCLTCH KKSRTFEAFM
960 970 980 990 1000
YLSLPLASTS KCTLQDCLRL FSKEEKLTDN NRFYCSHCRA RRDSLKKIEI
1010 1020 1030 1040 1050
WKLPPVLLVH LKRFSYDGRW KQKLQTSVDF PLENLDLSQY VIGPKNNLKK
1060 1070 1080 1090 1100
YNLFSVSNHY GGLDGGHYTA YCKNAARQRW FKFDDHEVSD ISVSSVKSSA
1110 1118
AYILFYTSLG PRVTDVAT

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node
to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1733 Collapse assay details

Data source: Panorama

Official Gene Symbol
USP8
Peptide Modified Sequence
C[+57.0]PT[+80.0]PEIQK
Modification Type
Carbamidomethyl (C), Phospho (ST)
Protein - Site of Modification
575, 577
Peptide - Site of Modification
1, 3
Peptide Start
575
Peptide End
582
CPTAC ID
CPTAC-1733
Peptide Molecular Mass
1,051.4409
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM or SRM
Enrichment Method
Titanium Dioxide
Matrix
Ovarian cancer cell lysate
Submitting Laboratory
Pacific Northwest National Laboratory
Submitting Lab PI
Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Waters nanoACQUITY (Part Number 176016000)
Column Packing
Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions
100 um x 100 mm
Flow Rate
0.5 uL/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-TiO2SRM


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (2+) 31.5 6.5 4.8 25.9 10.3 7.9 40.8 12.2 9.2 15 15 15
y3 (1+) 14.4 6.1 5.6 22.4 7.3 6 26.6 9.5 8.2 15 15 15
y5 (1+) 9.1 3 2.5 10.5 5.6 3.4 13.9 6.4 4.2 15 15 15
sum 10.8 2.3 1.3 11.3 4 3.2 15.6 4.6 3.5 15 15 15

Additional Resources and Comments


Assay Details for CPTAC-1734 Collapse assay details

Data source: Panorama

Official Gene Symbol
USP8
Peptide Modified Sequence
SYSS[+80.0]PDITQAIQEEEK
Modification Type
Phospho (ST)
Protein - Site of Modification
719
Peptide - Site of Modification
4
Peptide Start
716
Peptide End
731
CPTAC ID
CPTAC-1734
Peptide Molecular Mass
1,903.8088
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM or SRM
Enrichment Method
Titanium Dioxide
Matrix
Ovarian cancer cell lysate
Submitting Laboratory
Pacific Northwest National Laboratory
Submitting Lab PI
Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Waters nanoACQUITY (Part Number 176016000)
Column Packing
Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions
100 um x 100 mm
Flow Rate
0.5 uL/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-TiO2SRM


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (1+) 6.2 4.2 7 6.3 4.4 8.3 8.8 6.1 10.9 15 15 15
y6 (1+) 8.1 9.1 9.1 10.5 8.7 10.4 13.3 12.6 13.8 15 15 15
y7 (1+) 8.3 6.4 3.2 7 8.6 5.4 10.9 10.7 6.3 15 15 15
sum 4.6 3 4 5.3 4.2 5.5 7 5.2 6.8 15 15 15

Additional Resources and Comments