CABLES1, CPTAC-1741 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
CABLES1	CABLES

Sequence Length (AA)	Molecular Weight (Da)
633	67599

Protein Name
CDK5 and ABL1 enzyme substrate 1

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MAAAAAAATT	AACSSGSAGT	DAAGASGLQQ	PPPQPQPQPA	AAAPAQPPPE
60	70	80	90	100
PPRKPRMDPR	RRQAALSFLT	NISLDGRLPP	QDAEWGGGEE	GGAAKPGAGG
110	120	130	140	150
ACGARTRFSL	LAAAERGGCI	ALAAPGTPAA	GLAAGSGPCL	PQPSSLPPLI
160	170	180	190	200
PGGHATVSGP	GVARGFASPL	GAGRASGEQW	QPPRPAPLAA	CAQLQLLDGS
210	220	230	240	250
GAAGQEELEE	DDAFISVQVP	AAAFLGSGTP	GSGSGSRGRL	NSFTQGILPI
260	270	280	290	300
AFSRPTSQNY	CSLEQPGQGG	STSAFEQLQR	SRRRLISQRS	SLETLEDIEE
310	320	330	340	350
NAPLRRCRTL	SGSPRPKNFK	KIHFIKNMRQ	HDTRNGRIVL	ISGRRSFCSI
360	370	380	390	400
FSVLPYRDST	QVGDLKLDGG	RQSTGAVSLK	EIIGLEGVEL	GADGKTVSYT
410	420	430	440	450
QFLLPTNAFG	ARRNTIDSTS	SFSQFRNLSH	RSLSIGRASG	TQGSLDTGSD
460	470	480	490	500
LGDFMDYDPN	LLDDPQWPCG	KHKRVLIFPS	YMTTVIDYVK	PSDLKKDMNE
510	520	530	540	550
TFKEKFPHIK	LTLSKIRSLK	REMRKLAQED	CGLEEPTVAM	AFVYFEKLAL
560	570	580	590	600
KGKLNKQNRK	LCAGACVLLA	AKIGSDLKKH	EVKHLIDKLE	EKFRLNRREL
610	620	630	633
IAFEFPVLVA	LEFALHLPEH	EVMPHYRRLV	QSS

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-1742 Collapse assay details

Data source: Panorama

Official Gene Symbol: CABLES1
Peptide Modified Sequence: NTIDSTSS[+80.0]FSQFR
Modification Type: Phospho (ST)
Protein - Site of Modification: 421
Peptide - Site of Modification: 8
Peptide Start: 414
Peptide End: 426

CPTAC ID: CPTAC-1742
Peptide Molecular Mass: 1,568.6508
Species: Homo sapiens (Human)
Assay Type: Enrichment MRM or SRM
Enrichment Method: Titanium Dioxide
Matrix: Ovarian cancer cell lysate
Submitting Laboratory: Pacific Northwest National Laboratory
Submitting Lab PI: Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: TSQ Vantage
Internal Standard: peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: Waters nanoACQUITY (Part Number 176016000)
Column Packing: Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions: 100 um x 100 mm
Flow Rate: 0.5 uL/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-TiO2SRM

CPTAC-1724: STRN. DVDELPSLQPSVGSPS[+80.0]RPSSSR	CPTAC-1725: STRN. DVDELPSLQPSVGSPSRPSSS[+80.0]R	CPTAC-1726: PAK2. YLSFT[+80.0]PPEK
CPTAC-1727: SRF. ALIQTC[+57.0]LNSPDS[+80.0]PPR	CPTAC-1728: STAT5A. AVDGY[+80.0]VKPQIK	CPTAC-1729: DOCK7. LGTGFNPNT[+80.0]LDK
CPTAC-1730: DOCK7. MNS[+80.0]LTFK	CPTAC-1731: DOCK7. SPS[+80.0]GSAFGSQENLR	CPTAC-1732: DOCK7. AVLPVT[+80.0]C[+57.0]HR
CPTAC-1733: USP8. C[+57.0]PT[+80.0]PEIQK	CPTAC-1734: USP8. SYSS[+80.0]PDITQAIQEEEK	CPTAC-1735: ARHGEF6. KPS[+80.0]EEEYVIR
CPTAC-1736: ARHGEF6. DS[+80.0]IPQVLLPEEEK	CPTAC-1737: STRN. S[+80.0]VIDTSTIVR	CPTAC-1738: PLCG1. GSDAS[+80.0]GQLFHGR
CPTAC-1739: NF2. LT[+80.0]LQSAK	CPTAC-1740: PIK3R1. LY[+80.0]EEYTR	CPTAC-1741: CABLES1. QS[+80.0]TGAVSLK
CPTAC-1742: CABLES1. NTIDSTSS[+80.0]FSQFR	CPTAC-1743: PRKCD. SDSASS[+80.0]EPVGIYQGFEK	CPTAC-1744: NOC2L. SPDKPGGS[+80.0]PSASR
CPTAC-1745: CTNNB1. S[+80.0]PQMVSAIVR	CPTAC-1746: STAT1. TELIS[+80.0]VSEVHPSR	CPTAC-1747: RALBP1. TPS[+80.0]SEEISPTK
CPTAC-1748: RALBP1. TPSSEEIS[+80.0]PTK	CPTAC-1749: NFATC4. T[+80.0]SSEQAVALPR	CPTAC-1750: CTNNA1. T[+80.0]SVQTEDDQLIAGQSAR
CPTAC-1751: STAT3. YC[+57.0]RPESQEHPEADPGSAAPY[+80.0]LK

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y2 (1+)	7	4.5	4.8	12.3	4.6	10	14.2	6.4	11.1	15	15	15
y4 (1+)	18.5	5.5	5.3	19.1	7	6.2	26.6	8.9	8.2	15	15	15
y9 (1+)	28.9	14.5	4.8	31	18.4	11	42.4	23.4	12	15	15	15
sum	11.1	3	3.9	11.1	4.6	6.6	15.7	5.5	7.7	15	15	15

Additional Resources and Comments

Resources

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View Peptide in Panorama

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Comments

Assay Details for CPTAC-1741 Collapse assay details

Data source: Panorama

Official Gene Symbol: CABLES1
Peptide Modified Sequence: QS[+80.0]TGAVSLK
Modification Type: Phospho (ST)
Protein - Site of Modification: 373
Peptide - Site of Modification: 2
Peptide Start: 372
Peptide End: 380

CPTAC ID: CPTAC-1741
Peptide Molecular Mass: 969.4532
Species: Homo sapiens (Human)
Assay Type: Enrichment MRM or SRM
Enrichment Method: Titanium Dioxide
Matrix: Ovarian cancer cell lysate
Submitting Laboratory: Pacific Northwest National Laboratory
Submitting Lab PI: Tao Liu