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Overview Data source: UniProt

Official Gene Symbol Other Aliases
ARHGEF6 COOL2, KIAA0006, PIXA
Sequence Length (AA) Molecular Weight (Da)
776 87499
Protein Name
Rho guanine nucleotide exchange factor 6
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MNPEEQIVTW LISLGVLESP KKTICDPEEF LKSSLKNGVV LCKLINRLMP
60 70 80 90 100
GSVEKFCLDP QTEADCINNI NDFLKGCATL QVEIFDPDDL YSGVNFSKVL
110 120 130 140 150
STLLAVNKAT EDQLSERPCG RSSSLSAANT SQTNPQGAVS STVSGLQRQS
160 170 180 190 200
KTVEMTENGS HQLIVKARFN FKQTNEDELS VCKGDIIYVT RVEEGGWWEG
210 220 230 240 250
TLNGRTGWFP SNYVREIKSS ERPLSPKAVK GFETAPLTKN YYTVVLQNIL
260 270 280 290 300
DTEKEYAKEL QSLLVTYLRP LQSNNNLSTV EVTSLLGNFE EVCTFQQTLC
310 320 330 340 350
QALEECSKFP ENQHKVGGCL LSLMPHFKSM YLAYCANHPS AVNVLTQHSD
360 370 380 390 400
ELEQFMENQG ASSPGILILT TNLSKPFMRL EKYVTLLQEL ERHMEDTHPD
410 420 430 440 450
HQDILKAIVA FKTLMGQCQD LRKRKQLELQ ILSEPIQAWE GEDIKNLGNV
460 470 480 490 500
IFMSQVMVQY GACEEKEERY LMLFSNVLIM LSASPRMSGF IYQGKIPIAG
510 520 530 540 550
TVVTRLDEIE GNDCTFEITG NTVERIVVHC NNNQDFQEWL EQLNRLIRGP
560 570 580 590 600
ASCSSLSKTS SSSCSAHSSF SSTGQPRGPL EPPQIIKPWS LSCLRPAPPL
610 620 630 640 650
RPSAALGYKE RMSYILKESS KSPKTMKKFL HKRKTERKPS EEEYVIRKST
660 670 680 690 700
AALEEDAQIL KVIEAYCTSA NFQQGHGSST RKDSIPQVLL PEEEKLIIEE
710 720 730 740 750
TRSNGQTIME EKSLVDTVYA LKDEVRELKQ ENKRMKQCLE EELKSRRDLE
760 770 776
KLVRRLLKQT DECIRGESSS KTSILP

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1736 Collapse assay details

Data source: Panorama

Official Gene Symbol
ARHGEF6
Peptide Modified Sequence
DS[+80.0]IPQVLLPEEEK
Modification Type
Phospho (ST)
Protein - Site of Modification
684
Peptide - Site of Modification
2
Peptide Start
683
Peptide End
695
CPTAC ID
CPTAC-1736
Peptide Molecular Mass
1,575.7433
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM or SRM
Enrichment Method
Titanium Dioxide
Matrix
Ovarian cancer cell lysate
Submitting Laboratory
Pacific Northwest National Laboratory
Submitting Lab PI
Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Waters nanoACQUITY (Part Number 176016000)
Column Packing
Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions
100 um x 100 mm
Flow Rate
0.5 uL/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-TiO2SRM


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y10 (2+) 3.1 1.1 1 3.1 2.8 1.6 4.4 3 1.9 15 15 15
y5 (1+) 5.6 1.6 1 6.6 2.9 2 8.7 3.3 2.2 15 15 15
y6 (1+) 5.3 2 1.7 7.7 2.7 1.8 9.3 3.4 2.5 15 15 15
sum 2.4 1 0.4 3.8 2.6 1.3 4.5 2.8 1.4 15 15 15

Additional Resources and Comments


Assay Details for CPTAC-1735 Collapse assay details

Data source: Panorama

Official Gene Symbol
ARHGEF6
Peptide Modified Sequence
KPS[+80.0]EEEYVIR
Modification Type
Phospho (ST)
Protein - Site of Modification
640
Peptide - Site of Modification
3
Peptide Start
638
Peptide End
647
CPTAC ID
CPTAC-1735
Peptide Molecular Mass
1,328.6013
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM or SRM
Enrichment Method
Titanium Dioxide
Matrix
Ovarian cancer cell lysate
Submitting Laboratory
Pacific Northwest National Laboratory
Submitting Lab PI
Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Waters nanoACQUITY (Part Number 176016000)
Column Packing
Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions
100 um x 100 mm
Flow Rate
0.5 uL/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-TiO2SRM


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 15 9.9 6.7 11.4 13.3 6 18.8 16.6 9 15 15 15
y8-98 (1+) 6.7 5.6 3.9 9.1 7.7 6 11.3 9.5 7.2 15 15 15
NA (NA+) 8.8 3.9 5.2 10.3 6.3 7.7 13.5 7.4 9.3 15 15 15
sum 6.9 3.7 3.7 8.3 6.1 6.2 10.8 7.1 7.2 15 15 15

Additional Resources and Comments