ARHGEF6, CPTAC-1735 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
ARHGEF6	COOL2, KIAA0006, PIXA

Sequence Length (AA)	Molecular Weight (Da)
776	87499

Protein Name
Rho guanine nucleotide exchange factor 6

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MNPEEQIVTW	LISLGVLESP	KKTICDPEEF	LKSSLKNGVV	LCKLINRLMP
60	70	80	90	100
GSVEKFCLDP	QTEADCINNI	NDFLKGCATL	QVEIFDPDDL	YSGVNFSKVL
110	120	130	140	150
STLLAVNKAT	EDQLSERPCG	RSSSLSAANT	SQTNPQGAVS	STVSGLQRQS
160	170	180	190	200
KTVEMTENGS	HQLIVKARFN	FKQTNEDELS	VCKGDIIYVT	RVEEGGWWEG
210	220	230	240	250
TLNGRTGWFP	SNYVREIKSS	ERPLSPKAVK	GFETAPLTKN	YYTVVLQNIL
260	270	280	290	300
DTEKEYAKEL	QSLLVTYLRP	LQSNNNLSTV	EVTSLLGNFE	EVCTFQQTLC
310	320	330	340	350
QALEECSKFP	ENQHKVGGCL	LSLMPHFKSM	YLAYCANHPS	AVNVLTQHSD
360	370	380	390	400
ELEQFMENQG	ASSPGILILT	TNLSKPFMRL	EKYVTLLQEL	ERHMEDTHPD
410	420	430	440	450
HQDILKAIVA	FKTLMGQCQD	LRKRKQLELQ	ILSEPIQAWE	GEDIKNLGNV
460	470	480	490	500
IFMSQVMVQY	GACEEKEERY	LMLFSNVLIM	LSASPRMSGF	IYQGKIPIAG
510	520	530	540	550
TVVTRLDEIE	GNDCTFEITG	NTVERIVVHC	NNNQDFQEWL	EQLNRLIRGP
560	570	580	590	600
ASCSSLSKTS	SSSCSAHSSF	SSTGQPRGPL	EPPQIIKPWS	LSCLRPAPPL
610	620	630	640	650
RPSAALGYKE	RMSYILKESS	KSPKTMKKFL	HKRKTERKPS	EEEYVIRKST
660	670	680	690	700
AALEEDAQIL	KVIEAYCTSA	NFQQGHGSST	RKDSIPQVLL	PEEEKLIIEE
710	720	730	740	750
TRSNGQTIME	EKSLVDTVYA	LKDEVRELKQ	ENKRMKQCLE	EELKSRRDLE
760	770	776
KLVRRLLKQT	DECIRGESSS	KTSILP

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-1736 Collapse assay details

Data source: Panorama

Official Gene Symbol: ARHGEF6
Peptide Modified Sequence: DS[+80.0]IPQVLLPEEEK
Modification Type: Phospho (ST)
Protein - Site of Modification: 684
Peptide - Site of Modification: 2
Peptide Start: 683
Peptide End: 695

CPTAC ID: CPTAC-1736
Peptide Molecular Mass: 1,575.7433
Species: Homo sapiens (Human)
Assay Type: Enrichment MRM or SRM
Enrichment Method: Titanium Dioxide
Matrix: Ovarian cancer cell lysate
Submitting Laboratory: Pacific Northwest National Laboratory
Submitting Lab PI: Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: TSQ Vantage
Internal Standard: peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Waters nanoACQUITY (Part Number 176016000)
Column Packing: Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions: 100 um x 100 mm
Flow Rate: 0.5 uL/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-TiO2SRM

CPTAC-1724: STRN. DVDELPSLQPSVGSPS[+80.0]RPSSSR	CPTAC-1725: STRN. DVDELPSLQPSVGSPSRPSSS[+80.0]R	CPTAC-1726: PAK2. YLSFT[+80.0]PPEK
CPTAC-1727: SRF. ALIQTC[+57.0]LNSPDS[+80.0]PPR	CPTAC-1728: STAT5A. AVDGY[+80.0]VKPQIK	CPTAC-1729: DOCK7. LGTGFNPNT[+80.0]LDK
CPTAC-1730: DOCK7. MNS[+80.0]LTFK	CPTAC-1731: DOCK7. SPS[+80.0]GSAFGSQENLR	CPTAC-1732: DOCK7. AVLPVT[+80.0]C[+57.0]HR
CPTAC-1733: USP8. C[+57.0]PT[+80.0]PEIQK	CPTAC-1734: USP8. SYSS[+80.0]PDITQAIQEEEK	CPTAC-1735: ARHGEF6. KPS[+80.0]EEEYVIR
CPTAC-1736: ARHGEF6. DS[+80.0]IPQVLLPEEEK	CPTAC-1737: STRN. S[+80.0]VIDTSTIVR	CPTAC-1738: PLCG1. GSDAS[+80.0]GQLFHGR
CPTAC-1739: NF2. LT[+80.0]LQSAK	CPTAC-1740: PIK3R1. LY[+80.0]EEYTR	CPTAC-1741: CABLES1. QS[+80.0]TGAVSLK
CPTAC-1742: CABLES1. NTIDSTSS[+80.0]FSQFR	CPTAC-1743: PRKCD. SDSASS[+80.0]EPVGIYQGFEK	CPTAC-1744: NOC2L. SPDKPGGS[+80.0]PSASR
CPTAC-1745: CTNNB1. S[+80.0]PQMVSAIVR	CPTAC-1746: STAT1. TELIS[+80.0]VSEVHPSR	CPTAC-1747: RALBP1. TPS[+80.0]SEEISPTK
CPTAC-1748: RALBP1. TPSSEEIS[+80.0]PTK	CPTAC-1749: NFATC4. T[+80.0]SSEQAVALPR	CPTAC-1750: CTNNA1. T[+80.0]SVQTEDDQLIAGQSAR
CPTAC-1751: STAT3. YC[+57.0]RPESQEHPEADPGSAAPY[+80.0]LK

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y10 (2+)	3.1	1.1	1	3.1	2.8	1.6	4.4	3	1.9	15	15	15
y5 (1+)	5.6	1.6	1	6.6	2.9	2	8.7	3.3	2.2	15	15	15
y6 (1+)	5.3	2	1.7	7.7	2.7	1.8	9.3	3.4	2.5	15	15	15
sum	2.4	1	0.4	3.8	2.6	1.3	4.5	2.8	1.4	15	15	15

Additional Resources and Comments

Resources

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View Peptide in Panorama

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Comments

Assay Details for CPTAC-1735 Collapse assay details

Data source: Panorama

Official Gene Symbol: ARHGEF6
Peptide Modified Sequence: KPS[+80.0]EEEYVIR
Modification Type: Phospho (ST)
Protein - Site of Modification: 640
Peptide - Site of Modification: 3
Peptide Start: 638
Peptide End: 647

CPTAC ID: CPTAC-1735
Peptide Molecular Mass: 1,328.6013
Species: Homo sapiens (Human)
Assay Type: Enrichment MRM or SRM
Enrichment Method: Titanium Dioxide
Matrix: Ovarian cancer cell lysate
Submitting Laboratory: Pacific Northwest National Laboratory
Submitting Lab PI: Tao Liu