NOC2L, CPTAC-1744 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
NOC2L	NIR

Sequence Length (AA)	Molecular Weight (Da)
749	84919

Protein Name
Nucleolar complex protein 2 homolog

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MAAAGSRKRR	LAELTVDEFL	ASGFDSESES	ESENSPQAET	REAREAARSP
60	70	80	90	100
DKPGGSPSAS	RRKGRASEHK	DQLSRLKDRD	PEFYKFLQEN	DQSLLNFSDS
110	120	130	140	150
DSSEEEEGPF	HSLPDVLEEA	SEEEDGAEEG	EDGDRVPRGL	KGKKNSVPVT
160	170	180	190	200
VAMVERWKQA	AKQRLTPKLF	HEVVQAFRAA	VATTRGDQES	AEANKFQVTD
210	220	230	240	250
SAAFNALVTF	CIRDLIGCLQ	KLLFGKVAKD	SSRMLQPSSS	PLWGKLRVDI
260	270	280	290	300
KAYLGSAIQL	VSCLSETTVL	AAVLRHISVL	VPCFLTFPKQ	CRMLLKRMVI
310	320	330	340	350
VWSTGEESLR	VLAFLVLSRV	CRHKKDTFLG	PVLKQMYITY	VRNCKFTSPG
360	370	380	390	400
ALPFISFMQW	TLTELLALEP	GVAYQHAFLY	IRQLAIHLRN	AMTTRKKETY
410	420	430	440	450
QSVYNWQYVH	CLFLWCRVLS	TAGPSEALQP	LVYPLAQVII	GCIKLIPTAR
460	470	480	490	500
FYPLRMHCIR	ALTLLSGSSG	AFIPVLPFIL	EMFQQVDFNR	KPGRMSSKPI
510	520	530	540	550
NFSVILKLSN	VNLQEKAYRD	GLVEQLYDLT	LEYLHSQAHC	IGFPELVLPV
560	570	580	590	600
VLQLKSFLRE	CKVANYCRQV	QQLLGKVQEN	SAYICSRRQR	VSFGVSEQQA
610	620	630	640	650
VEAWEKLTRE	EGTPLTLYYS	HWRKLRDREI	QLEISGKERL	EDLNFPEIKR
660	670	680	690	700
RKMADRKDED	RKQFKDLFDL	NSSEEDDTEG	FSERGILRPL	STRHGVEDDE
710	720	730	740	749
EDEEEGEEDS	SNSEDGDPDA	EAGLAPGELQ	QLAQGPEDEL	EDLQLSEDD

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-1744 Collapse assay details

Data source: Panorama

Official Gene Symbol: NOC2L
Peptide Modified Sequence: SPDKPGGS[+80.0]PSASR
Modification Type: Phospho (ST)
Protein - Site of Modification: 56
Peptide - Site of Modification: 8
Peptide Start: 49
Peptide End: 61

CPTAC ID: CPTAC-1744
Peptide Molecular Mass: 1,321.5664
Species: Homo sapiens (Human)
Assay Type: Enrichment MRM or SRM
Enrichment Method: Titanium Dioxide
Matrix: Ovarian cancer cell lysate
Submitting Laboratory: Pacific Northwest National Laboratory
Submitting Lab PI: Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: TSQ Vantage
Internal Standard: peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: Waters nanoACQUITY (Part Number 176016000)
Column Packing: Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions: 100 um x 100 mm
Flow Rate: 0.5 uL/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-TiO2SRM

CPTAC-1724: STRN. DVDELPSLQPSVGSPS[+80.0]RPSSSR	CPTAC-1725: STRN. DVDELPSLQPSVGSPSRPSSS[+80.0]R	CPTAC-1726: PAK2. YLSFT[+80.0]PPEK
CPTAC-1727: SRF. ALIQTC[+57.0]LNSPDS[+80.0]PPR	CPTAC-1728: STAT5A. AVDGY[+80.0]VKPQIK	CPTAC-1729: DOCK7. LGTGFNPNT[+80.0]LDK
CPTAC-1730: DOCK7. MNS[+80.0]LTFK	CPTAC-1731: DOCK7. SPS[+80.0]GSAFGSQENLR	CPTAC-1732: DOCK7. AVLPVT[+80.0]C[+57.0]HR
CPTAC-1733: USP8. C[+57.0]PT[+80.0]PEIQK	CPTAC-1734: USP8. SYSS[+80.0]PDITQAIQEEEK	CPTAC-1735: ARHGEF6. KPS[+80.0]EEEYVIR
CPTAC-1736: ARHGEF6. DS[+80.0]IPQVLLPEEEK	CPTAC-1737: STRN. S[+80.0]VIDTSTIVR	CPTAC-1738: PLCG1. GSDAS[+80.0]GQLFHGR
CPTAC-1739: NF2. LT[+80.0]LQSAK	CPTAC-1740: PIK3R1. LY[+80.0]EEYTR	CPTAC-1741: CABLES1. QS[+80.0]TGAVSLK
CPTAC-1742: CABLES1. NTIDSTSS[+80.0]FSQFR	CPTAC-1743: PRKCD. SDSASS[+80.0]EPVGIYQGFEK	CPTAC-1744: NOC2L. SPDKPGGS[+80.0]PSASR
CPTAC-1745: CTNNB1. S[+80.0]PQMVSAIVR	CPTAC-1746: STAT1. TELIS[+80.0]VSEVHPSR	CPTAC-1747: RALBP1. TPS[+80.0]SEEISPTK
CPTAC-1748: RALBP1. TPSSEEIS[+80.0]PTK	CPTAC-1749: NFATC4. T[+80.0]SSEQAVALPR	CPTAC-1750: CTNNA1. T[+80.0]SVQTEDDQLIAGQSAR
CPTAC-1751: STAT3. YC[+57.0]RPESQEHPEADPGSAAPY[+80.0]LK

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y9-98 (1+)	12.4	6.5	8.1	18.9	8.8	10.5	22.6	10.9	13.3	15	15	15
y11 (2+)	49.1	17.3	13.2	41.1	18.8	16.8	64	25.5	21.4	15	15	15
y10 (2+)	21.2	21	12.7	23.2	21.5	14.5	31.4	30.1	19.3	15	15	15
sum	15.9	5	7.4	19.7	11	11.3	25.3	12.1	13.5	15	15	15

NOC2L

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-1744 Collapse assay details

Assay Parameters Collapse assay parameters

Assay Multiplexing Expand assay panel

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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NOC2L

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-1744 Collapse assay details

Assay Parameters Collapse assay parameters

Assay Multiplexing Expand assay panel

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments