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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
CTNNB1 CTNNB
Sequence Length (AA) Molecular Weight (Da)
781 85497
Protein Name
Catenin beta-1
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MATQADLMEL DMAMEPDRKA AVSHWQQQSY LDSGIHSGAT TTAPSLSGKG
60 70 80 90 100
NPEEEDVDTS QVLYEWEQGF SQSFTQEQVA DIDGQYAMTR AQRVRAAMFP
110 120 130 140 150
ETLDEGMQIP STQFDAAHPT NVQRLAEPSQ MLKHAVVNLI NYQDDAELAT
160 170 180 190 200
RAIPELTKLL NDEDQVVVNK AAVMVHQLSK KEASRHAIMR SPQMVSAIVR
210 220 230 240 250
TMQNTNDVET ARCTAGTLHN LSHHREGLLA IFKSGGIPAL VKMLGSPVDS
260 270 280 290 300
VLFYAITTLH NLLLHQEGAK MAVRLAGGLQ KMVALLNKTN VKFLAITTDC
310 320 330 340 350
LQILAYGNQE SKLIILASGG PQALVNIMRT YTYEKLLWTT SRVLKVLSVC
360 370 380 390 400
SSNKPAIVEA GGMQALGLHL TDPSQRLVQN CLWTLRNLSD AATKQEGMEG
410 420 430 440 450
LLGTLVQLLG SDDINVVTCA AGILSNLTCN NYKNKMMVCQ VGGIEALVRT
460 470 480 490 500
VLRAGDREDI TEPAICALRH LTSRHQEAEM AQNAVRLHYG LPVVVKLLHP
510 520 530 540 550
PSHWPLIKAT VGLIRNLALC PANHAPLREQ GAIPRLVQLL VRAHQDTQRR
560 570 580 590 600
TSMGGTQQQF VEGVRMEEIV EGCTGALHIL ARDVHNRIVI RGLNTIPLFV
610 620 630 640 650
QLLYSPIENI QRVAAGVLCE LAQDKEAAEA IEAEGATAPL TELLHSRNEG
660 670 680 690 700
VATYAAAVLF RMSEDKPQDY KKRLSVELTS SLFRTEPMAW NETADLGLDI
710 720 730 740 750
GAQGEPLGYR QDDPSYRSFH SGGYGQDALG MDPMMEHEMG GHHPGADYPV
760 770 780 781
DGLPDLGHAQ DLMDGLPPGD SNQLAWFDTD L

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node
to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3265 Collapse assay details

Data source: Panorama

Official Gene Symbol
CTNNB1
Peptide Sequence
RTSMGGTQQQFVEGVR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
550
Peptide End
565
CPTAC ID
non-CPTAC-3265
Peptide Molecular Mass
1,779.8686
Species
Homo sapiens (Human)
Assay Type
Direct MRM or SRM
Matrix
Cell Line Pool: H2444, H2122, AsPC-1, BxPC-3
Submitting Laboratory
Moffitt Cancer Center
Submitting Lab PI
John Koomen

Publication

View Details (opens in a new window)

Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Thermo TSQ Quantiva
Internal Standard
light
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Dionex Ultimate 3000 RSLCnano System
Column Packing
C18, 2 uM, 100 A
Column Dimensions
75 uM x 25 cm
Flow Rate
300 nl/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-TiO2SRM


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 11.1 4.8 1.6 15.1 12.5 7.4 18.7 13.4 7.6 15 15 15
y6 (1+) 7.2 3.8 2.6 13.7 10.6 8.4 15.5 11.3 8.8 15 15 15
y5 (1+) 9.7 3.7 3.1 11.2 9.7 7.5 14.8 10.4 8.1 15 15 15
y4 (1+) 7 3 2.3 10 9.7 9.9 12.2 10.2 10.2 15 15 15
sum 4.6 3.1 1.3 10.3 10 8.2 11.3 10.5 8.3 15 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-5716 Collapse assay details

Data source: Panorama

Official Gene Symbol
CTNNB1
Peptide Modified Sequence
RTS[+79.966331]MGGTQQQFVEGVR
Modification Type
Phospho (ST)
Protein - Site of Modification
N/A
Peptide - Site of Modification
3
Peptide Start
550
Peptide End
565
CPTAC ID
non-CPTAC-5716
Peptide Molecular Mass
1,859.8349
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM or SRM
Enrichment Method
Titanium Dioxide
Matrix
Cell Lysate
Submitting Laboratory
Moffitt Cancer Center
Submitting Lab PI
John Koomen

Publication

View Details (opens in a new window)

Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Thermo Quantiva
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Dionex Ultimate 3000 RSLCnano
Column Packing
Pepmap 100
Column Dimensions
75um x 25cm
Flow Rate
300 nL/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-TiO2SRM


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 4.4 3.4 2.3 6.1 3.9 3.1 7.5 5.2 3.9 15 15 15
y4 (1+) 5.6 2.9 2.6 6 4 2.6 8.2 4.9 3.7 15 15 15
y6 (1+) 2.6 3.2 3.2 2.3 3.6 3.4 3.5 4.8 4.7 15 15 15
y7 (1+) 7 5.2 2.5 5.4 4.8 3.1 8.8 7.1 4 15 15 15
sum 2.7 2.1 2.1 2.4 2.7 2.4 3.6 3.4 3.2 15 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-3266 Collapse assay details

Data source: Panorama

Official Gene Symbol
CTNNB1
Peptide Sequence
TSMGGTQQQFVEGVR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
551
Peptide End
565
CPTAC ID
non-CPTAC-3266
Peptide Molecular Mass
1,623.7675
Species
Homo sapiens (Human)
Assay Type
Direct MRM or SRM
Matrix
Cell Line Pool: H2444, H2122, AsPC-1, BxPC-3
Submitting Laboratory
Moffitt Cancer Center
Submitting Lab PI
John Koomen

Publication

View Details (opens in a new window)

Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Thermo TSQ Quantiva
Internal Standard
light
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Dionex Ultimate 3000 RSLCnano System
Column Packing
C18, 2 uM, 100 A
Column Dimensions
75 uM x 25 cm
Flow Rate
300 nl/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-TiO2SRM


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (1+) 9.9 3.4 1.6 11.8 9.3 10 15.4 9.9 10.1 15 15 15
y7 (1+) 8.1 4.3 2.4 14.6 10.2 9.2 16.7 11.1 9.5 15 15 15
y6 (1+) 8.3 4.2 2.4 15.2 10.9 10.1 17.3 11.7 10.4 15 15 15
y4 (1+) 7.3 2.5 1.3 15.4 10.2 8.5 17 10.5 8.6 15 15 15
y3 (1+) 5.3 2.7 1.4 11.6 10.4 9.1 12.8 10.7 9.2 15 15 15
sum 1.9 1.8 0.9 12.2 10 9 12.3 10.2 9 15 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-5365 Collapse assay details

Data source: Panorama

Official Gene Symbol
CTNNB1
Peptide Sequence
TSMGGTQQQFVEGVR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
551
Peptide End
565
CPTAC ID
non-CPTAC-5365
Peptide Molecular Mass
1,623.7675
Species
Homo sapiens (Human)
Assay Type
Direct MRM or SRM
Matrix
Cell Lysate
Submitting Laboratory
Moffitt Cancer Center
Submitting Lab PI
John Koomen

Publication

View Details (opens in a new window)

Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
RSLCnano and Quantiva
Internal Standard
Stable Isotope Labeled Standard
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
RSLCnano
Column Packing
C18 PepMap 100
Column Dimensions
75 micron ID x 25 cm length
Flow Rate
300 nl/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-TiO2SRM


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y4 (1+) 8.1 3.3 2.8 8.7 4 2.9 11.9 5.2 4 15 15 15
y6 (1+) 6.8 2.8 3.1 9.8 3.1 4.5 11.9 4.2 5.5 15 15 15
y7 (1+) 8.9 3.8 3.4 9.7 5 3.3 13.2 6.3 4.7 15 15 15
y8 (1+) 12.2 4.4 3.4 13.3 3.4 4.8 18 5.6 5.9 15 15 15
y3 (1+) 4.9 2.3 1.6 7.4 3 2.6 8.9 3.8 3.1 15 15 15
sum 4.8 1.2 1.7 6.1 2.2 2.8 7.8 2.5 3.3 15 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-5715 Collapse assay details

Data source: Panorama

Official Gene Symbol
CTNNB1
Peptide Modified Sequence
TS[+79.966331]MGGTQQQFVEGVR
Modification Type
Phospho (ST)
Protein - Site of Modification
N/A
Peptide - Site of Modification
3
Peptide Start
551
Peptide End
565
CPTAC ID
non-CPTAC-5715
Peptide Molecular Mass
1,703.7338
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM or SRM
Enrichment Method
Titanium Dioxide
Matrix
Cell Lysate
Submitting Laboratory
Moffitt Cancer Center
Submitting Lab PI
John Koomen

Publication

View Details (opens in a new window)

Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Thermo Quantiva
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Dionex Ultimate 3000 RSLCnano
Column Packing
Pepmap 100
Column Dimensions
75um x 25cm
Flow Rate
300 nL/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-TiO2SRM


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 10.1 4.8 3.3 9.7 5.3 4.5 14 7.2 5.6 15 15 15
y4 (1+) 8.5 4.8 2.6 8.9 6.6 5.4 12.3 8.2 6 15 15 15
y5 (1+) 7.5 5.7 1.9 6.9 7.4 3.4 10.2 9.3 3.9 15 15 15
y8 (1+) 11.2 4.3 3.1 10.7 4.8 3.9 15.5 6.4 5 15 15 15
sum 5.7 2.6 1.6 6.7 3.2 4 8.8 4.1 4.3 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-1745 Collapse assay details

Data source: Panorama

Official Gene Symbol
CTNNB1
Peptide Modified Sequence
S[+80.0]PQMVSAIVR
Modification Type
Phospho (ST)
Protein - Site of Modification
191
Peptide - Site of Modification
1
Peptide Start
191
Peptide End
200
CPTAC ID
CPTAC-1745
Peptide Molecular Mass
1,166.5519
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM or SRM
Enrichment Method
Titanium Dioxide
Matrix
Ovarian cancer cell lysate
Submitting Laboratory
Pacific Northwest National Laboratory
Submitting Lab PI
Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Waters nanoACQUITY (Part Number 176016000)
Column Packing
Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions
100 um x 100 mm
Flow Rate
0.5 uL/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-TiO2SRM


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (1+) 23.6 5.4 7.2 26.3 6.2 12.1 35.3 8.2 14.1 15 15 15
y6 (1+) 23.1 9.1 7 21.6 10.9 9.9 31.6 14.2 12.1 15 15 15
y7 (1+) 17.9 6.8 3.9 18.7 6.9 5.2 25.9 9.7 6.5 15 15 15
sum 14.5 4 3.1 15.8 5.1 4.9 21.4 6.5 5.8 15 15 15


Additional Resources and Comments