PAK2, CPTAC-1726 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
PAK2	N/A

Sequence Length (AA)	Molecular Weight (Da)
524	58043

Protein Name
Serine/threonine-protein kinase PAK 2

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MSDNGELEDK	PPAPPVRMSS	TIFSTGGKDP	LSANHSLKPL	PSVPEEKKPR
60	70	80	90	100
HKIISIFSGT	EKGSKKKEKE	RPEISPPSDF	EHTIHVGFDA	VTGEFTGMPE
110	120	130	140	150
QWARLLQTSN	ITKLEQKKNP	QAVLDVLKFY	DSNTVKQKYL	SFTPPEKDGF
160	170	180	190	200
PSGTPALNAK	GTEAPAVVTE	EEDDDEETAP	PVIAPRPDHT	KSIYTRSVID
210	220	230	240	250
PVPAPVGDSH	VDGAAKSLDK	QKKKTKMTDE	EIMEKLRTIV	SIGDPKKKYT
260	270	280	290	300
RYEKIGQGAS	GTVFTATDVA	LGQEVAIKQI	NLQKQPKKEL	IINEILVMKE
310	320	330	340	350
LKNPNIVNFL	DSYLVGDELF	VVMEYLAGGS	LTDVVTETCM	DEAQIAAVCR
360	370	380	390	400
ECLQALEFLH	ANQVIHRDIK	SDNVLLGMEG	SVKLTDFGFC	AQITPEQSKR
410	420	430	440	450
STMVGTPYWM	APEVVTRKAY	GPKVDIWSLG	IMAIEMVEGE	PPYLNENPLR
460	470	480	490	500
ALYLIATNGT	PELQNPEKLS	PIFRDFLNRC	LEMDVEKRGS	AKELLQHPFL
510	520	524
KLAKPLSSLT	PLIMAAKEAM	KSNR

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt

Assay Details for CPTAC-1628 Collapse assay details

Data source: Panorama

Official Gene Symbol: PAK2
Peptide Sequence: SVIDPVPAPVGDSHVDGAAK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 197
Peptide End: 216

CPTAC ID: CPTAC-1628
Peptide Molecular Mass: 1,929.9796
Species: Homo sapiens (Human)
Assay Type: Direct MRM or SRM
Matrix: Ovarian cancer tumor tissue lysate
Submitting Laboratory: Pacific Northwest National Laboratory
Submitting Lab PI: Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: TSQ Vantage
Internal Standard: peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Waters nanoACQUITY (Part Number 176016000)
Column Packing: Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions: 100 um x 100 mm
Flow Rate: 0.5 uL/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-TiO2SRM

CPTAC-1724: STRN. DVDELPSLQPSVGSPS[+80.0]RPSSSR	CPTAC-1725: STRN. DVDELPSLQPSVGSPSRPSSS[+80.0]R	CPTAC-1726: PAK2. YLSFT[+80.0]PPEK
CPTAC-1727: SRF. ALIQTC[+57.0]LNSPDS[+80.0]PPR	CPTAC-1728: STAT5A. AVDGY[+80.0]VKPQIK	CPTAC-1729: DOCK7. LGTGFNPNT[+80.0]LDK
CPTAC-1730: DOCK7. MNS[+80.0]LTFK	CPTAC-1731: DOCK7. SPS[+80.0]GSAFGSQENLR	CPTAC-1732: DOCK7. AVLPVT[+80.0]C[+57.0]HR
CPTAC-1733: USP8. C[+57.0]PT[+80.0]PEIQK	CPTAC-1734: USP8. SYSS[+80.0]PDITQAIQEEEK	CPTAC-1735: ARHGEF6. KPS[+80.0]EEEYVIR
CPTAC-1736: ARHGEF6. DS[+80.0]IPQVLLPEEEK	CPTAC-1737: STRN. S[+80.0]VIDTSTIVR	CPTAC-1738: PLCG1. GSDAS[+80.0]GQLFHGR
CPTAC-1739: NF2. LT[+80.0]LQSAK	CPTAC-1740: PIK3R1. LY[+80.0]EEYTR	CPTAC-1741: CABLES1. QS[+80.0]TGAVSLK
CPTAC-1742: CABLES1. NTIDSTSS[+80.0]FSQFR	CPTAC-1743: PRKCD. SDSASS[+80.0]EPVGIYQGFEK	CPTAC-1744: NOC2L. SPDKPGGS[+80.0]PSASR
CPTAC-1745: CTNNB1. S[+80.0]PQMVSAIVR	CPTAC-1746: STAT1. TELIS[+80.0]VSEVHPSR	CPTAC-1747: RALBP1. TPS[+80.0]SEEISPTK
CPTAC-1748: RALBP1. TPSSEEIS[+80.0]PTK	CPTAC-1749: NFATC4. T[+80.0]SSEQAVALPR	CPTAC-1750: CTNNA1. T[+80.0]SVQTEDDQLIAGQSAR
CPTAC-1751: STAT3. YC[+57.0]RPESQEHPEADPGSAAPY[+80.0]LK

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y12 (2+)	4.4	1.5	1.1	13.2	5	5.2	13.9	5.2	5.3	15	15	15
y14 (2+)	8.4	2.7	1.8	17.9	5	6.5	19.8	5.7	6.7	15	15	15
y12 (1+)	10.8	2.4	2.6	11.9	5.1	6.2	16.1	5.6	6.7	15	15	15
sum	3.9	1.7	0.8	13.2	4.9	5.5	13.8	5.2	5.6	15	15	15

Additional Resources and Comments

Resources

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View Peptide in Panorama

View Protein in Panorama

Comments

Assay Details for CPTAC-1726 Collapse assay details

Data source: Panorama

Official Gene Symbol: PAK2
Peptide Modified Sequence: YLSFT[+80.0]PPEK
Modification Type: Phospho (ST)
Protein - Site of Modification: 143
Peptide - Site of Modification: 5
Peptide Start: 139
Peptide End: 147

CPTAC ID: CPTAC-1726
Peptide Molecular Mass: 1,160.5155
Species: Homo sapiens (Human)
Assay Type: Enrichment MRM or SRM
Enrichment Method: Titanium Dioxide
Matrix: Ovarian cancer cell lysate
Submitting Laboratory: Pacific Northwest National Laboratory
Submitting Lab PI: Tao Liu