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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
STRN N/A
Sequence Length (AA) Molecular Weight (Da)
780 86132
Protein Name
Striatin
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MDEQAGPGVF FSNNHPGAGG AKGLGPLAEA AAAGDGAAAA GAARAQYSLP
60 70 80 90 100
GILHFLQHEW ARFEVERAQW EVERAELQAQ IAFLQGERKG QENLKKDLVR
110 120 130 140 150
RIKMLEYALK QERAKYHKLK YGTELNQGDM KPPSYDSDEG NETEVQPQQN
160 170 180 190 200
SQLMWKQGRQ LLRQYLQEVG YTDTILDVKS KRVRALLGFS SDVTDREDDK
210 220 230 240 250
NQDSVVNGTE AEVKETAMIA KSELTDSASV LDNFKFLESA AADFSDEDED
260 270 280 290 300
DDVDGREKSV IDTSTIVRKK ALPDSGEDRD TKEALKEFDF LVTSEEGDNE
310 320 330 340 350
SRSAGDGTDW EKEDQCLMPE AWNVDQGVIT KLKEQYKKER KGKKGVKRPN
360 370 380 390 400
RSKLQDMLAN LRDVDELPSL QPSVGSPSRP SSSRLPEHEI NRADEVEALT
410 420 430 440 450
FPPSSGKSFI MGADEALESE LGLGELAGLT VANEADSLTY DIANNKDALR
460 470 480 490 500
KTWNPKFTLR SHFDGIRALA FHPIEPVLIT ASEDHTLKMW NLQKTAPAKK
510 520 530 540 550
STSLDVEPIY TFRAHKGPVL CVVMSSNGEQ CYSGGTDGLI QGWNTTNPNI
560 570 580 590 600
DPYDSYDPSV LRGPLLGHTD AVWGLAYSAA HQRLLSCSAD GTLRLWNTTE
610 620 630 640 650
VAPALSVFND TKELGIPASV DLVSSDPSHM VASFSKGYTS IFNMETQQRI
660 670 680 690 700
LTLESNVDTT ANSSCQINRV ISHPTLPISI TAHEDRHIKF YDNNTGKLIH
710 720 730 740 750
SMVAHLEAVT SLAVDPNGLY LMSGSHDCSI RLWNLESKTC IQEFTAHRKK
760 770 780
FEESIHDVAF HPSKCYIASA GADALAKVFV

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1724 Collapse assay details

Data source: Panorama

Official Gene Symbol
STRN
Peptide Modified Sequence
DVDELPSLQPSVGSPS[+80.0]RPSSSR
Modification Type
Phospho (ST)
Protein - Site of Modification
378
Peptide - Site of Modification
16
Peptide Start
363
Peptide End
384
CPTAC ID
CPTAC-1724
Peptide Molecular Mass
2,376.0958
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM or SRM
Enrichment Method
Titanium Dioxide
Matrix
Ovarian cancer cell lysate
Submitting Laboratory
Pacific Northwest National Laboratory
Submitting Lab PI
Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Waters nanoACQUITY (Part Number 176016000)
Column Packing
Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions
100 um x 100 mm
Flow Rate
0.5 uL/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-TiO2SRM


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b4 (1+) 20.3 9.4 13.4 16.1 10.3 10.8 25.9 13.9 17.2 15 15 15
y16 (2+) 21.5 7.9 14 19.3 8.9 12.2 28.9 11.9 18.6 15 15 15
y5 (1+) 30.4 12.5 15.9 28.6 15.2 17.1 41.7 19.7 23.3 12 15 15
sum 14.6 5.6 8.2 12.9 5.5 7.3 19.5 7.8 11 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-1725 Collapse assay details

Data source: Panorama

Official Gene Symbol
STRN
Peptide Modified Sequence
DVDELPSLQPSVGSPSRPSSS[+80.0]R
Modification Type
Phospho (ST)
Protein - Site of Modification
383
Peptide - Site of Modification
21
Peptide Start
363
Peptide End
384
CPTAC ID
CPTAC-1725
Peptide Molecular Mass
2,376.0958
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM or SRM
Enrichment Method
Titanium Dioxide
Matrix
Ovarian cancer cell lysate
Submitting Laboratory
Pacific Northwest National Laboratory
Submitting Lab PI
Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Waters nanoACQUITY (Part Number 176016000)
Column Packing
Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions
100 um x 100 mm
Flow Rate
0.5 uL/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-TiO2SRM


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b4 (1+) 17.8 8.9 10 21.6 10.3 11.1 28 13.6 14.9 15 15 15
y16 (2+) 19.5 8.5 4.9 22.5 8.7 8.2 29.8 12.2 9.6 15 15 15
y17 (2+) 29.9 19.1 36.3 33.9 20.3 35.5 45.2 27.9 50.8 15 15 15
sum 11.5 6.5 4.9 16.3 7.6 6.7 19.9 10 8.3 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-1640 Collapse assay details

Data source: Panorama

Official Gene Symbol
STRN
Peptide Sequence
SELTDSASVLDNFK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
222
Peptide End
235
CPTAC ID
CPTAC-1640
Peptide Molecular Mass
1,524.7308
Species
Homo sapiens (Human)
Assay Type
Direct MRM or SRM
Matrix
Ovarian cancer tumor tissue lysate
Submitting Laboratory
Pacific Northwest National Laboratory
Submitting Lab PI
Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Waters nanoACQUITY (Part Number 176016000)
Column Packing
Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions
100 um x 100 mm
Flow Rate
0.5 uL/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-TiO2SRM


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 4.1 2.4 4.1 11.1 5.8 5.4 11.8 6.3 6.8 15 15 15
y10 (1+) 4.4 5.3 4.7 12.1 8.1 7.7 12.9 9.7 9 15 15 15
y11 (1+) 3.7 2.8 8.9 10.7 8.9 8.2 11.3 9.3 12.1 15 15 15
sum 2.8 1.8 4.4 11 7 5.8 11.4 7.2 7.3 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-1737 Collapse assay details

Data source: Panorama

Official Gene Symbol
STRN
Peptide Modified Sequence
S[+80.0]VIDTSTIVR
Modification Type
Phospho (ST)
Protein - Site of Modification
259
Peptide - Site of Modification
1
Peptide Start
259
Peptide End
268
CPTAC ID
CPTAC-1737
Peptide Molecular Mass
1,169.5693
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM or SRM
Enrichment Method
Titanium Dioxide
Matrix
Ovarian cancer cell lysate
Submitting Laboratory
Pacific Northwest National Laboratory
Submitting Lab PI
Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Waters nanoACQUITY (Part Number 176016000)
Column Packing
Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions
100 um x 100 mm
Flow Rate
0.5 uL/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-TiO2SRM


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (1+) 29.6 7.8 9.6 25.2 8.1 8.1 38.9 11.2 12.6 15 15 15
y6 (1+) 25 5.5 5.7 16.7 4.8 5.6 30.1 7.3 8 15 15 15
y7 (1+) 11.2 4 3.7 9.5 4.2 4.5 14.7 5.8 5.8 15 15 15
sum 11.2 3.3 3 9.6 3.4 3.6 14.8 4.7 4.7 15 15 15


Additional Resources and Comments